LOCUS       sequence-360275-        6583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TALED_Left-ND1-1397C-AD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Cho SI, Lee S, Mok YG, Lim K, Lee J, Lee JM, Chung E, Kim JS
  TITLE     Targeted A-to-G base editing in human mitochondrial DNA with 
            programmable deaminases.
  JOURNAL   Cell. 2022 May 12;185(10):1764-1776.e12. doi: 
            10.1016/j.cell.2022.03.039. Epub 2022 Apr 25.
  PUBMED    35472302
REFERENCE   2  (bases 1 to 6583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03.039"; journalName: "Cell"; date: 
            "2022-05-12- 12"; volume: "185"; issue: "10"; pages: "1764-1776.e12"
COMMENT     Sequence Label: TALED_Left-ND1-1397C-AD
FEATURES             Location/Qualifiers
     source          1..6583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(101..120)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        292..671
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        672..875
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     826..846
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     920..939
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        920..938
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1035..1061
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1065..1091
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1095..1121
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3852..3869)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3858..4082
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    4313..4434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4350..4369)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4404..4423
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4483..4499)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4483..4499)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4496..4518)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4507..4523
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4531..4537,4538..4555,4556..4561))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4576..4597
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4714..4731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4885..5473)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4965..4984)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5644..6435,6436..6504))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6267..6286
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(6505..6583,1..26))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
       61 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
      121 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
      181 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
      241 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
      301 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      361 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      421 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      481 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      541 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      601 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      661 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      721 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      781 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      841 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg
      901 cttactggct tatcgaaatt aatacgactc actataggga gatggctctt agtcgagccg
      961 tctgtggtac ttcccgacag cttgctccag tcttggggta ccttggctcc cgacaaaagc
     1021 actctctccc tgattaccct tacgatgtac ctgactacgc cggctaccct tacgacgtac
     1081 cagattacgc tggatatcca tacgatgtcc ctgattatgc tgggatccga attcaagatc
     1141 tacgcacgct cggctacagc cagcagcaac aggagaagat caaaccgaag gttcgttcga
     1201 cagtggcgca gcaccacgag gcactggtcg gccatgggtt tacacacgcg cacatcgttg
     1261 cgctcagcca acacccggca gcgttaggga ccgtcgctgt caagtatcag gacatgatcg
     1321 cagcgttgcc agaggcgaca cacgaagcga tcgttggcgt cggcaaacag tggtccggcg
     1381 cacgcgctct ggaggccttg ctcacggtgg cgggagagtt gagaggtcca ccgttacagt
     1441 tggacacagg ccaacttctc aagattgcaa aacgtggcgg cgtgaccgca gtggaggcag
     1501 tgcatgcatg gcgcaatgca ctgacgggtg cccccctgaa cctcactcct gatcaagtcg
     1561 tcgctattgc atcacacgat ggtggaaagc aggctctcga gacagtccaa cggctgttgc
     1621 ctgttctgtg ccaagcacat ggattgaccc cagatcaagt tgtggccatc gcaagtaacg
     1681 gaggcgggaa gcaagcactt gaaaccgtcc aaagactgtt gcctgtcctt tgccaggatc
     1741 acggcctcac acccgcccag gttgttgcta ttgcaagcaa catcggaggg aaacaagctt
     1801 tggaaaccgt gcagcgcctt ttgccagttc tctgtcaaga tcacgggttg acaccagatc
     1861 aggtggtggc cattgcaagc aataatggtg gcaagcaagc cctcgaaacc gtccaaaggc
     1921 tccttcctgt cttgtgtcag gcacatggcc tcactccaga ccaggtggtg gcaatcgctt
     1981 ctcatgatgg aggaaaacaa gcacttgaga ctgttcagcg gcttttgcct gttctttgcc
     2041 aagaccatgg acttacccca gaccaggtcg ttgccatcgc ctcacatgac ggtggaaagc
     2101 aggccttgga aacagtgcaa cgcttgctcc ccgttctgtg ccaagctcat ggtttgacac
     2161 cagcacaggt tgtcgccatc gctagcaatg ggggtggcaa acaggcactt gaaacagtgc
     2221 aacggttgtt gcccgtcttg tgtcaggacc atgggcttac ccctgcacag gtcgtggcaa
     2281 ttgcctccaa catcgggggg aagcaagccc ttgaaactgt ccagcgcctg ttgccagttc
     2341 tttgtcaagc ccatggattg actcccgacc aggtggtcgc aattgcaagt aacaacggtg
     2401 ggaaacaggc tttggagact gtgcagagac ttcttcccgt gttgtgccaa gcacatggcc
     2461 tcaccccagc tcaggttgtt gctattgcat ctcacgacgg cggaaagcaa gctctcgaga
     2521 cagtccagag gttgcttcct gtcctttgcc aggaccacgg tcttacacct gaccaagttg
     2581 tcgccatcgc ctcccatgat ggtgggaaac aggccctgga aactgtgcag cgccttctgc
     2641 ctgtcctctg tcaggcccac ggattgacac ccgagcaggt ggtcgccatt gcttctaaca
     2701 acgggggcaa gcaggcactt gagactgtcc agaggctcct ccccgttctt tgtcaagcac
     2761 atggcctgac tccagcccaa gttgtcgcca ttgccagtaa tggtggtggt aagcaggccc
     2821 tggagactgt gcaaaggctt ctgccagttt tgtgccaagc tcatggtctg actccagccc
     2881 aagttgtcgc cattgccagt aatggtggtg gtaagcaggc cctggagact gtgcaaaggc
     2941 ttctgccagt tttgtgccaa gcacacggtc tgactccgga acaggtggtg gcgattgcaa
     3001 gcaacggcgg cggcaaacag gctctagaga gcattgttgc ccagctctcc agacctgatc
     3061 cggcgctagc cgcgttgaca aatgatcatc ttgttgctct tgcttgtctt ggcggacgac
     3121 ctgccctgga tgctgtgaaa aaggggttgc tagtcggcag tgccatacct gtgaagcggg
     3181 gagcaacagg ggagacaaag gtgttcacag gcaactctaa cagtccaaag agccccacca
     3241 aaggcgggtg ttcaggaagc gaaactcctg gtacctcaga gtccgctact cccgaatcct
     3301 ctgaggtgga gttttcccac gagtactgga tgagacatgc cctgaccctg gccaagaggg
     3361 cacgggatga gagggaggtg cctgtgggag ccgtgctggt gctgaacaat agagtgatcg
     3421 gcgagggctg gaacagagcc atcggcctgc acgacccaac agcccatgcc gaaattatgg
     3481 ccctgagaca gggcggcctg gtcatgcaga actacagact gattgacgcc accctgtacg
     3541 tgacattcga gccttgcgtg atgtgcgccg gcgccatgat ccactctagg atcggccgcg
     3601 tggtgtttgg cgtgaggaac tcaaaaagag gcgccgcagg ctccctgatg aacgtgctga
     3661 actaccccgg catgaatcac cgcgtcgaaa ttaccgaggg aatcctggca gatgaatgtg
     3721 ccgccctgct gtgcgatttc tatcggatgc ctagacaggt gttcaatgct cagaagaagg
     3781 cccagagctc catcaactag tcagggccct attctatagg tgtcacctaa atgctagagc
     3841 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3901 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     3961 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     4021 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     4081 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg ctgcagtgag
     4141 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     4201 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     4261 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     4321 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     4381 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     4441 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     4501 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     4561 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     4621 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     4681 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4741 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4801 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4861 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4921 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4981 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5041 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5101 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5161 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5221 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5281 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     5341 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5401 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5461 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5521 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5581 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5641 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5701 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5761 ccccagtgct gcaatgatac cgcgagatcc acgctcaccg gctccagatt tatcagcaat
     5821 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     5881 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     5941 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6001 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6061 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6121 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6181 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6241 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6301 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     6361 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     6421 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     6481 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     6541 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tag
//
