LOCUS       sequence-360276-        6300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TALED_Left-ND1-1397C-UGI in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Cho SI, Lee S, Mok YG, Lim K, Lee J, Lee JM, Chung E, Kim JS
  TITLE     Targeted A-to-G base editing in human mitochondrial DNA with 
            programmable deaminases.
  JOURNAL   Cell. 2022 May 12;185(10):1764-1776.e12. doi: 
            10.1016/j.cell.2022.03.039. Epub 2022 Apr 25.
  PUBMED    35472302
REFERENCE   2  (bases 1 to 6300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03.039"; journalName: "Cell"; date: 
            "2022-05-12- 12"; volume: "185"; issue: "10"; pages: "1764-1776.e12"
COMMENT     Sequence Label: TALED_Left-ND1-1397C-UGI
FEATURES             Location/Qualifiers
     source          1..6300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(99..118)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        290..669
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        670..873
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     824..844
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     918..937
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        918..936
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1033..1059
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1063..1089
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1093..1119
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3265..3516
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     promoter        complement(3529..3547)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3530..3547)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3567..3584)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3573..3797
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    4028..4149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4065..4084)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4119..4138
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4198..4214)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4198..4214)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4211..4233)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4222..4238
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4246..4252,4253..4270,4271..4276))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4291..4312
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4429..4446)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4600..5188)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4680..4699)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5359..6150,6151..6219))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5982..6001
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(6220..6300,1..24))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
       61 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
      121 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
      181 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
      241 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
      301 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      361 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      421 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      481 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      541 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      601 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      661 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      721 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      781 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      841 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      901 tactggctta tcgaaattaa tacgactcac tatagggaga tggctcttag tcgagccgtc
      961 tgtggtactt cccgacagct tgctccagtc ttggggtacc ttggctcccg acaaaagcac
     1021 tctctccctg attaccctta cgatgtacct gactacgccg gctaccctta cgacgtacca
     1081 gattacgctg gatatccata cgatgtccct gattatgctg ggatccgaat tcaagatcta
     1141 cgcacgctcg gctacagcca gcagcaacag gagaagatca aaccgaaggt tcgttcgaca
     1201 gtggcgcagc accacgaggc actggtcggc catgggttta cacacgcgca catcgttgcg
     1261 ctcagccaac acccggcagc gttagggacc gtcgctgtca agtatcagga catgatcgca
     1321 gcgttgccag aggcgacaca cgaagcgatc gttggcgtcg gcaaacagtg gtccggcgca
     1381 cgcgctctgg aggccttgct cacggtggcg ggagagttga gaggtccacc gttacagttg
     1441 gacacaggcc aacttctcaa gattgcaaaa cgtggcggcg tgaccgcagt ggaggcagtg
     1501 catgcatggc gcaatgcact gacgggtgcc cccctgaacc tcactcctga tcaagtcgtc
     1561 gctattgcat cacacgatgg tggaaagcag gctctcgaga cagtccaacg gctgttgcct
     1621 gttctgtgcc aagcacatgg attgacccca gatcaagttg tggccatcgc aagtaacgga
     1681 ggcgggaagc aagcacttga aaccgtccaa agactgttgc ctgtcctttg ccaggatcac
     1741 ggcctcacac ccgcccaggt tgttgctatt gcaagcaaca tcggagggaa acaagctttg
     1801 gaaaccgtgc agcgcctttt gccagttctc tgtcaagatc acgggttgac accagatcag
     1861 gtggtggcca ttgcaagcaa taatggtggc aagcaagccc tcgaaaccgt ccaaaggctc
     1921 cttcctgtct tgtgtcaggc acatggcctc actccagacc aggtggtggc aatcgcttct
     1981 catgatggag gaaaacaagc acttgagact gttcagcggc ttttgcctgt tctttgccaa
     2041 gaccatggac ttaccccaga ccaggtcgtt gccatcgcct cacatgacgg tggaaagcag
     2101 gccttggaaa cagtgcaacg cttgctcccc gttctgtgcc aagctcatgg tttgacacca
     2161 gcacaggttg tcgccatcgc tagcaatggg ggtggcaaac aggcacttga aacagtgcaa
     2221 cggttgttgc ccgtcttgtg tcaggaccat gggcttaccc ctgcacaggt cgtggcaatt
     2281 gcctccaaca tcggggggaa gcaagccctt gaaactgtcc agcgcctgtt gccagttctt
     2341 tgtcaagccc atggattgac tcccgaccag gtggtcgcaa ttgcaagtaa caacggtggg
     2401 aaacaggctt tggagactgt gcagagactt cttcccgtgt tgtgccaagc acatggcctc
     2461 accccagctc aggttgttgc tattgcatct cacgacggcg gaaagcaagc tctcgagaca
     2521 gtccagaggt tgcttcctgt cctttgccag gaccacggtc ttacacctga ccaagttgtc
     2581 gccatcgcct cccatgatgg tgggaaacag gccctggaaa ctgtgcagcg ccttctgcct
     2641 gtcctctgtc aggcccacgg attgacaccc gagcaggtgg tcgccattgc ttctaacaac
     2701 gggggcaagc aggcacttga gactgtccag aggctcctcc ccgttctttg tcaagcacat
     2761 ggcctgactc cagcccaagt tgtcgccatt gccagtaatg gtggtggtaa gcaggccctg
     2821 gagactgtgc aaaggcttct gccagttttg tgccaagctc atggtctgac tccagcccaa
     2881 gttgtcgcca ttgccagtaa tggtggtggt aagcaggccc tggagactgt gcaaaggctt
     2941 ctgccagttt tgtgccaagc acacggtctg actccggaac aggtggtggc gattgcaagc
     3001 aacggcggcg gcaaacaggc tctagagagc attgttgccc agctctccag acctgatccg
     3061 gcgctagccg cgttgacaaa tgatcatctt gttgctcttg cttgtcttgg cggacgacct
     3121 gccctggatg ctgtgaaaaa ggggttgggg ggctctggca gtgccatacc tgtgaagcgg
     3181 ggagcaacag gggagacaaa ggtgttcaca ggcaactcta acagtccaaa gagccccacc
     3241 aaaggcgggt gttccggtgg ttcaacaaat ctgtctgaca tcatagaaaa agaaactggc
     3301 aaacaactgg tgatacaaga aagtattctg atgttgcctg aggaagttga agaggtaata
     3361 ggcaacaagc ctgaaagcga tatactggta cataccgcat acgatgagtc aactgacgaa
     3421 aacgtgatgc tcttgacatc cgatgcccct gaatacaagc cctgggcact tgtaatccaa
     3481 gatagtaatg gggagaacaa aatcaaaatg ttgtagtcag ggccctattc tatagtgtca
     3541 cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     3601 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     3661 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     3721 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     3781 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     3841 ccacgctgca gtgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3901 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3961 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     4021 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4081 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4141 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     4201 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     4261 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     4321 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     4381 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4441 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4501 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4561 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4621 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4681 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4741 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4801 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4861 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4921 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4981 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5041 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5101 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5161 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5221 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5281 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5341 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5401 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5461 gggcttacca tctggcccca gtgctgcaat gataccgcga gatccacgct caccggctcc
     5521 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5581 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5641 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5701 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5761 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5821 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5881 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5941 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6001 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6061 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6121 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6181 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6241 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
//
