LOCUS       sequence-360279-        6840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TALED_Left-ND1-AD-E1347A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Cho SI, Lee S, Mok YG, Lim K, Lee J, Lee JM, Chung E, Kim JS
  TITLE     Targeted A-to-G base editing in human mitochondrial DNA with 
            programmable deaminases.
  JOURNAL   Cell. 2022 May 12;185(10):1764-1776.e12. doi: 
            10.1016/j.cell.2022.03.039. Epub 2022 Apr 25.
  PUBMED    35472302
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03.039"; journalName: "Cell"; date: 
            "2022-05-12- 12"; volume: "185"; issue: "10"; pages: "1764-1776.e12"
COMMENT     Sequence Label: TALED_Left-ND1-AD-E1347A
FEATURES             Location/Qualifiers
     source          1..6840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(88..107)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        279..658
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        659..862
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     813..833
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     907..926
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        907..925
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1022..1048
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1052..1078
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1082..1108
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(4058..4076)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4059..4076)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4096..4113)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4102..4326
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    4557..4678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4594..4613)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4648..4667
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4727..4743)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4727..4743)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4740..4762)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4751..4767
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4775..4781,4782..4799,4800..4805))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4820..4841
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4958..4975)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5129..5717)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5209..5228)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5888..6679,6680..6748))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6511..6530
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(6749..6840,1..13))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
       61 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
      121 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
      181 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
      241 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
      301 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      361 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      421 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      481 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      541 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      601 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      661 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      721 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      781 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      841 ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt actggcttat
      901 cgaaattaat acgactcact atagggagat ggctcttagt cgagccgtct gtggtacttc
      961 ccgacagctt gctccagtct tggggtacct tggctcccga caaaagcact ctctccctga
     1021 ttacccttac gatgtacctg actacgccgg ctacccttac gacgtaccag attacgctgg
     1081 atatccatac gatgtccctg attatgctgg gatccgaatt caagatctac gcacgctcgg
     1141 ctacagccag cagcaacagg agaagatcaa accgaaggtt cgttcgacag tggcgcagca
     1201 ccacgaggca ctggtcggcc atgggtttac acacgcgcac atcgttgcgc tcagccaaca
     1261 cccggcagcg ttagggaccg tcgctgtcaa gtatcaggac atgatcgcag cgttgccaga
     1321 ggcgacacac gaagcgatcg ttggcgtcgg caaacagtgg tccggcgcac gcgctctgga
     1381 ggccttgctc acggtggcgg gagagttgag aggtccaccg ttacagttgg acacaggcca
     1441 acttctcaag attgcaaaac gtggcggcgt gaccgcagtg gaggcagtgc atgcatggcg
     1501 caatgcactg acgggtgccc ccctgaacct cactcctgat caagtcgtcg ctattgcatc
     1561 acacgatggt ggaaagcagg ctctcgagac agtccaacgg ctgttgcctg ttctgtgcca
     1621 agcacatgga ttgaccccag atcaagttgt ggccatcgca agtaacggag gcgggaagca
     1681 agcacttgaa accgtccaaa gactgttgcc tgtcctttgc caggatcacg gcctcacacc
     1741 cgcccaggtt gttgctattg caagcaacat cggagggaaa caagctttgg aaaccgtgca
     1801 gcgccttttg ccagttctct gtcaagatca cgggttgaca ccagatcagg tggtggccat
     1861 tgcaagcaat aatggtggca agcaagccct cgaaaccgtc caaaggctcc ttcctgtctt
     1921 gtgtcaggca catggcctca ctccagacca ggtggtggca atcgcttctc atgatggagg
     1981 aaaacaagca cttgagactg ttcagcggct tttgcctgtt ctttgccaag accatggact
     2041 taccccagac caggtcgttg ccatcgcctc acatgacggt ggaaagcagg ccttggaaac
     2101 agtgcaacgc ttgctccccg ttctgtgcca agctcatggt ttgacaccag cacaggttgt
     2161 cgccatcgct agcaatgggg gtggcaaaca ggcacttgaa acagtgcaac ggttgttgcc
     2221 cgtcttgtgt caggaccatg ggcttacccc tgcacaggtc gtggcaattg cctccaacat
     2281 cggggggaag caagcccttg aaactgtcca gcgcctgttg ccagttcttt gtcaagccca
     2341 tggattgact cccgaccagg tggtcgcaat tgcaagtaac aacggtggga aacaggcttt
     2401 ggagactgtg cagagacttc ttcccgtgtt gtgccaagca catggcctca ccccagctca
     2461 ggttgttgct attgcatctc acgacggcgg aaagcaagct ctcgagacag tccagaggtt
     2521 gcttcctgtc ctttgccagg accacggtct tacacctgac caagttgtcg ccatcgcctc
     2581 ccatgatggt gggaaacagg ccctggaaac tgtgcagcgc cttctgcctg tcctctgtca
     2641 ggcccacgga ttgacacccg agcaggtggt cgccattgct tctaacaacg ggggcaagca
     2701 ggcacttgag actgtccaga ggctcctccc cgttctttgt caagcacatg gcctgactcc
     2761 agcccaagtt gtcgccattg ccagtaatgg tggtggtaag caggccctgg agactgtgca
     2821 aaggcttctg ccagttttgt gccaagctca tggtctgact ccagcccaag ttgtcgccat
     2881 tgccagtaat ggtggtggta agcaggccct ggagactgtg caaaggcttc tgccagtttt
     2941 gtgccaagca cacggtctga ctccggaaca ggtggtggcg attgcaagca acggcggcgg
     3001 caaacaggct ctagagagca ttgttgccca gctctccaga cctgatccgg cgctagccgc
     3061 gttgctagtc tcaggaagcg aaactcctgg tacctcagag tccgctactc ccgaatcctc
     3121 tgaggtggag ttttcccacg agtactggat gagacatgcc ctgaccctgg ccaagagggc
     3181 acgggatgag agggaggtgc ctgtgggagc cgtgctggtg ctgaacaata gagtgatcgg
     3241 cgagggctgg aacagagcca tcggcctgca cgacccaaca gcccatgccg aaattatggc
     3301 cctgagacag ggcggcctgg tcatgcagaa ctacagactg attgacgcca ccctgtacgt
     3361 gacattcgag ccttgcgtga tgtgcgccgg cgccatgatc cactctagga tcggccgcgt
     3421 ggtgtttggc gtgaggaact caaaaagagg cgccgcaggc tccctgatga acgtgctgaa
     3481 ctaccccggc atgaatcacc gcgtcgaaat taccgaggga atcctggcag atgaatgtgc
     3541 cgccctgctg tgcgatttct atcggatgcc tagacaggtg ttcaatgctc agaagaaggc
     3601 ccagagctcc atcaacctag tcggatccgg cagctacgcc ctgggtccgt atcagattag
     3661 cgccccgcag ctgccagctt acaatggtca gaccgtgggt accttctact atgtgaacga
     3721 cgcgggcggt ctggagagca aggtgtttag cagcggcggt ccaaccccgt acccaaacta
     3781 tgccaatgcc ggtcatgtgg ccggtcagag cgccctgttc atgcgtgata acggcatcag
     3841 cgagggtctg gtgttccaca acaacccgga aggcacctgc ggtttttgcg tgaacatgac
     3901 cgagaccctg ctgccggaaa acgcgaaaat gaccgtggtg ccgccggaag gtgccattcc
     3961 agtgaagcgc ggcgctaccg gtgaaaccaa agtgtttacc ggtaacagca acagcccgaa
     4021 gagcccgacc aaaggcggtt gctagtcagg gccctattct atagtgtcac ctaaatgcta
     4081 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4141 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4201 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4261 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     4321 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgctgcag
     4381 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     4441 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     4501 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     4561 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4621 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4681 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     4741 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     4801 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     4861 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     4921 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     4981 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5041 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5101 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5161 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5221 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5281 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5341 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5401 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5461 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5521 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     5581 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5641 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5701 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     5761 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     5821 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     5881 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5941 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6001 ctggccccag tgctgcaatg ataccgcgag atccacgctc accggctcca gatttatcag
     6061 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6121 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6181 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6241 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6301 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6361 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6421 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6481 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     6541 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6601 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6661 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6721 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6781 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
//
