LOCUS       sequence-360280-        6288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TALED_Left-ND1-E1347A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Cho SI, Lee S, Mok YG, Lim K, Lee J, Lee JM, Chung E, Kim JS
  TITLE     Targeted A-to-G base editing in human mitochondrial DNA with 
            programmable deaminases.
  JOURNAL   Cell. 2022 May 12;185(10):1764-1776.e12. doi: 
            10.1016/j.cell.2022.03.039. Epub 2022 Apr 25.
  PUBMED    35472302
REFERENCE   2  (bases 1 to 6288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03.039"; journalName: "Cell"; date: 
            "2022-05-12- 12"; volume: "185"; issue: "10"; pages: "1764-1776.e12"
COMMENT     Sequence Label: TALED_Left-ND1-E1347A
FEATURES             Location/Qualifiers
     source          1..6288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(529..1320,1321..1389))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1152..1171
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1390..1494)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1569..1588)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1760..2139
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2140..2343
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2294..2314
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2388..2407
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2388..2406
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2503..2529
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2533..2559
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2563..2589
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(4987..5005)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4988..5005)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5025..5042)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5031..5255
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    5486..5607
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5523..5542)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5577..5596
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5656..5672)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5656..5672)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5669..5691)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5680..5696
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5704..5710,5711..5728,5729..5734))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5749..5770
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5887..5904)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(6058..6288,1..358))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6138..6157)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
       61 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      121 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      181 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
      241 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
      301 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
      361 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
      421 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
      481 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
      541 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
      601 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
      661 gataccgcga gatccacgct caccggctcc agatttatca gcaataaacc agccagccgg
      721 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
      781 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
      841 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
      901 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
      961 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1021 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     1081 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     1141 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     1201 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     1261 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     1321 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     1381 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1441 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     1501 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     1561 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     1621 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     1681 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     1741 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     1801 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     1861 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     1921 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     1981 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2041 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2101 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2161 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     2221 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     2281 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     2341 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
     2401 tatagggaga tggctcttag tcgagccgtc tgtggtactt cccgacagct tgctccagtc
     2461 ttggggtacc ttggctcccg acaaaagcac tctctccctg attaccctta cgatgtacct
     2521 gactacgccg gctaccctta cgacgtacca gattacgctg gatatccata cgatgtccct
     2581 gattatgctg ggatccgaat tcaagatcta cgcacgctcg gctacagcca gcagcaacag
     2641 gagaagatca aaccgaaggt tcgttcgaca gtggcgcagc accacgaggc actggtcggc
     2701 catgggttta cacacgcgca catcgttgcg ctcagccaac acccggcagc gttagggacc
     2761 gtcgctgtca agtatcagga catgatcgca gcgttgccag aggcgacaca cgaagcgatc
     2821 gttggcgtcg gcaaacagtg gtccggcgca cgcgctctgg aggccttgct cacggtggcg
     2881 ggagagttga gaggtccacc gttacagttg gacacaggcc aacttctcaa gattgcaaaa
     2941 cgtggcggcg tgaccgcagt ggaggcagtg catgcatggc gcaatgcact gacgggtgcc
     3001 cccctgaacc tcactcctga tcaagtcgtc gctattgcat cacacgatgg tggaaagcag
     3061 gctctcgaga cagtccaacg gctgttgcct gttctgtgcc aagcacatgg attgacccca
     3121 gatcaagttg tggccatcgc aagtaacgga ggcgggaagc aagcacttga aaccgtccaa
     3181 agactgttgc ctgtcctttg ccaggatcac ggcctcacac ccgcccaggt tgttgctatt
     3241 gcaagcaaca tcggagggaa acaagctttg gaaaccgtgc agcgcctttt gccagttctc
     3301 tgtcaagatc acgggttgac accagatcag gtggtggcca ttgcaagcaa taatggtggc
     3361 aagcaagccc tcgaaaccgt ccaaaggctc cttcctgtct tgtgtcaggc acatggcctc
     3421 actccagacc aggtggtggc aatcgcttct catgatggag gaaaacaagc acttgagact
     3481 gttcagcggc ttttgcctgt tctttgccaa gaccatggac ttaccccaga ccaggtcgtt
     3541 gccatcgcct cacatgacgg tggaaagcag gccttggaaa cagtgcaacg cttgctcccc
     3601 gttctgtgcc aagctcatgg tttgacacca gcacaggttg tcgccatcgc tagcaatggg
     3661 ggtggcaaac aggcacttga aacagtgcaa cggttgttgc ccgtcttgtg tcaggaccat
     3721 gggcttaccc ctgcacaggt cgtggcaatt gcctccaaca tcggggggaa gcaagccctt
     3781 gaaactgtcc agcgcctgtt gccagttctt tgtcaagccc atggattgac tcccgaccag
     3841 gtggtcgcaa ttgcaagtaa caacggtggg aaacaggctt tggagactgt gcagagactt
     3901 cttcccgtgt tgtgccaagc acatggcctc accccagctc aggttgttgc tattgcatct
     3961 cacgacggcg gaaagcaagc tctcgagaca gtccagaggt tgcttcctgt cctttgccag
     4021 gaccacggtc ttacacctga ccaagttgtc gccatcgcct cccatgatgg tgggaaacag
     4081 gccctggaaa ctgtgcagcg ccttctgcct gtcctctgtc aggcccacgg attgacaccc
     4141 gagcaggtgg tcgccattgc ttctaacaac gggggcaagc aggcacttga gactgtccag
     4201 aggctcctcc ccgttctttg tcaagcacat ggcctgactc cagcccaagt tgtcgccatt
     4261 gccagtaatg gtggtggtaa gcaggccctg gagactgtgc aaaggcttct gccagttttg
     4321 tgccaagctc atggtctgac tccagcccaa gttgtcgcca ttgccagtaa tggtggtggt
     4381 aagcaggccc tggagactgt gcaaaggctt ctgccagttt tgtgccaagc acacggtctg
     4441 actccggaac aggtggtggc gattgcaagc aacggcggcg gcaaacaggc tctagagagc
     4501 attgttgccc agctctccag acctgatccg gcgctagccg cgttgctagt cggatccggc
     4561 agctacgccc tgggtccgta tcagattagc gccccgcagc tgccagctta caatggtcag
     4621 accgtgggta ccttctacta tgtgaacgac gcgggcggtc tggagagcaa ggtgtttagc
     4681 agcggcggtc caaccccgta cccaaactat gccaatgccg gtcatgtggc cggtcagagc
     4741 gccctgttca tgcgtgataa cggcatcagc gagggtctgg tgttccacaa caacccggaa
     4801 ggcacctgcg gtttttgcgt gaacatgacc gagaccctgc tgccggaaaa cgcgaaaatg
     4861 accgtggtgc cgccggaagg tgccattcca gtgaagcgcg gcgctaccgg tgaaaccaaa
     4921 gtgtttaccg gtaacagcaa cagcccgaag agcccgacca aaggcggttg ctagtcaggg
     4981 ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc
     5041 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     5101 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     5161 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     5221 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     5281 gggctctagg gggtatcccc acgctgcagt gagcgggact ctggggttcg aaatgaccga
     5341 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     5401 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     5461 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     5521 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5581 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     5641 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     5701 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     5761 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     5821 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     5881 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     5941 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6001 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6061 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6121 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6181 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6241 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggt
//
