LOCUS       sequence-360331-        4485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses codon-optimized full length SARS-CoV-2 Alpha Spike.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Benlarbi M, Laroche G, Fink C, Fu K, Mulloy RP, Phan A, Ariana A, 
            Stewart CM, Prevost J, Beaudoin-Bussieres G, Daniel R, Bo Y, El 
            Ferri O, Yockell-Lelievre J, Stanford WL, Giguere PM, Mubareka S, 
            Finzi A, Dekaban GA, Dikeakos JD, Cote M
  TITLE     Identification and differential usage of a host metalloproteinase 
            entry pathway by SARS-CoV-2 Delta and Omicron.
  JOURNAL   iScience. 2022 Nov 18;25(11):105316. doi: 
            10.1016/j.isci.2022.105316. Epub 2022 Oct 10.
  PUBMED    36254158
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-11-18"; volume: "25(11):105316. doi"; pages: " 
            10.1016/j.isci.2022.105316. Epub 2022 Oct 10"
COMMENT     Sequence Label: pCAGGS SARS-CoV-2 Alpha Spike
FEATURES             Location/Qualifiers
     source          1..4485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..29
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3885..3904)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3950..4005
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4004..4023)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4366..4382)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4366..4382)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4379..4401)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4390..4406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4414..4420,4421..4438,4439..4444))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4459..4480
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattcgcca
       61 ccatgttcgt cttcctcgtc ctcctccccc tcgtctcctc ccagtgcgtc aacctcacca
      121 cccgcaccca gctccccccc gcctacacca actccttcac ccgcggcgtc tactaccccg
      181 acaaggtctt ccgctcctcc gtcctccact ccacccagga cctcttcctc cccttcttct
      241 ccaacgtcac ctggttccac gccatctccg gcaccaacgg caccaagcgc ttcgacaacc
      301 ccgtcctccc cttcaacgac ggcgtctact tcgcctccac cgagaagtcc aacatcatcc
      361 gcggctggat cttcggcacc accctcgact ccaagaccca gtccctcctc atcgtcaaca
      421 acgccaccaa cgtcgtcatc aaggtctgcg agttccagtt ctgcaacgac cccttcctcg
      481 gcgtctacca caagaacaac aagtcctgga tggagtccga gttccgcgtc tactcctccg
      541 ccaacaactg caccttcgag tacgtctccc agcccttcct catggacctc gagggcaagc
      601 agggcaactt caagaacctc cgcgagttcg tcttcaagaa catcgacggc tacttcaaga
      661 tctactccaa gcacaccccc atcaacctcg tccgcgacct cccccagggc ttctccgccc
      721 tcgagcccct cgtcgacctc cccatcggca tcaacatcac ccgcttccag accctcctcg
      781 ccctccaccg ctcctacctc acccccggcg actcctcctc cggctggacc gccggcgccg
      841 ccgcctacta cgtcggctac ctccagcccc gcaccttcct cctcaagtac aacgagaacg
      901 gcaccatcac cgacgccgtc gactgcgccc tcgaccccct ctccgagacc aagtgcaccc
      961 tcaagtcctt caccgtcgag aagggcatct accagacctc caacttccgc gtccagccca
     1021 ccgagtccat cgtccgcttc cccaacatca ccaacctctg tcccttcggc gaggtcttca
     1081 acgccacccg cttcgcctcc gtctacgcct ggaaccgcaa gcgcatctcc aactgcgtcg
     1141 ccgactactc cgtcctctac aactccgcct ccttctccac cttcaagtgc tacggcgtct
     1201 cccccaccaa gctcaacgac ctctgcttca ccaacgtcta cgccgactcc ttcgtcatcc
     1261 gcggcgacga ggtccgccag atcgcccccg gccagaccgg caagatcgcc gactacaact
     1321 acaagctccc cgacgacttc accggctgcg tcatcgcctg gaactccaac aacctcgact
     1381 ccaaggtcgg cggcaactac aactacctct accgcctctt ccgcaagtcc aacctcaagc
     1441 ccttcgagcg cgacatctcc accgagatct accaggccgg ctccaccccc tgcaacggcg
     1501 tcgagggctt caactgctac ttccccctcc agtcctacgg cttccagccc acctacggcg
     1561 tcggctacca gccctaccgc gtcgtcgtcc tctccttcga gctcctccac gcccccgcca
     1621 ccgtctgcgg ccccaagaag tccaccaacc tcgtcaagaa caagtgcgtc aacttcaact
     1681 tcaacggcct caccggcacc ggcgtcctca ccgagtccaa caagaagttc ctccccttcc
     1741 agcagttcgg ccgcgacatc gacgacacca ccgacgccgt ccgcgacccc cagaccctcg
     1801 agatcctcga catcaccccc tgctccttcg gcggcgtctc cgtcatcacc cccggcacca
     1861 acacctccaa ccaggtcgcc gtcctctacc agggtgtcaa ctgcaccgag gtccccgtcg
     1921 ccatccacgc cgaccagctc acccccacct ggcgcgtcta ctccaccggc tccaacgtct
     1981 tccagacccg cgccggctgc ctcatcggcg ccgagcacgt caacaactcc tacgagtgcg
     2041 acatccccat cggcgccggc atctgcgcct cctaccagac ccagaccaac tcccaccgcc
     2101 gcgcccgctc cgtcgcctcc cagtccatca tcgcctacac catgtccctc ggcgccgaga
     2161 actccgtcgc ctactccaac aactccatcg ccatccccat caacttcacc atctccgtca
     2221 ccaccgagat cctccccgtc tccatgacca agacctccgt cgactgcacc atgtacatct
     2281 gcggcgactc caccgagtgc tccaacctcc tcctccagta cggctccttc tgcacccagc
     2341 tcaaccgcgc cctcaccggc atcgccgtcg agcaggacaa gaacacccag gaggtcttcg
     2401 cccaggtcaa gcagatctac aagacccccc ccatcaagga cttcggcggc ttcaacttct
     2461 cccagatcct ccccgacccc tccaagccct ccaagcgctc cttcatcgag gacctcctct
     2521 tcaacaaagt caccctcgcc gacgccggct tcatcaagca gtacggcgac tgcctcggcg
     2581 acatcgccgc ccgcgacctc atctgcgccc agaagttcaa cggcctcacc gtcctccccc
     2641 ccctcctcac cgacgagatg atcgcccagt acacctccgc cctcctcgcc ggcaccatca
     2701 cctccggctg gaccttcggc gccggcgccg ccctccagat ccccttcgcc atgcagatgg
     2761 cctaccgctt caacggcatc ggcgtcaccc agaacgtcct ctacgagaac cagaagctca
     2821 tcgccaacca gttcaactcc gccatcggca agatccagga ctccctctcc tccaccgcct
     2881 ccgccctcgg caagctccag gacgtcgtca accagaacgc ccaggccctc aacaccctcg
     2941 tcaagcagct ctcctccaac ttcggcgcca tctcctccgt cctcaacgac atcctcgccc
     3001 gcctcgacaa ggtcgaggcc gaggtccaga tcgaccgcct catcaccggc cgcctccagt
     3061 ccctccagac ctacgtcacc cagcagctca tccgcgccgc cgagatccgc gcctccgcca
     3121 acctcgccgc caccaagatg tccgagtgcg tcctcggcca gtccaagcgc gtcgacttct
     3181 gcggcaaggg ctaccacctc atgtccttcc cccagtccgc cccccacggc gtcgtcttcc
     3241 tccacgtcac ctacgtcccc gcccaggaga agaacttcac caccgccccc gccatctgcc
     3301 acgacggcaa ggcccacttc ccccgcgagg gcgtcttcgt ctccaacggc acccactggt
     3361 tcgtcaccca gcgcaacttc tacgagcccc agatcatcac cacccacaac accttcgtct
     3421 ccggcaactg cgacgtcgtc atcggcatcg tcaacaacac cgtctacgac cccctccagc
     3481 ccgagctcga ctccttcaag gaggagctcg acaagtactt caagaaccac acctcccccg
     3541 acgtcgacct cggcgacatc tccggcatca acgcctccgt cgtcaacatc cagaaggaga
     3601 tcgaccgcct caacgaggtc gccaagaacc tcaacgagtc cctcatcgac ctccaggagc
     3661 tcggcaagta cgagcagtac atcaagtggc cctggtacat ctggctcggc ttcatcgccg
     3721 gcctcatcgc catcgtcatg gtcaccatca tgctctgctg catgacctcc tgctgctcct
     3781 gcctcaaggg ctgctgctcc tgcggctcct gctgcaagtt cgacgaggac gactccgagc
     3841 ccgtcctcaa gggcgtcaag ctccactaca cctaagctag cagatctttt tccctctgcc
     3901 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
     3961 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
     4021 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
     4081 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa
     4141 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     4201 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
     4261 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
     4321 ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc
     4381 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4441 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgc
//
