LOCUS       sequence-360332-        4594 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses codon-optimized full length SARS-CoV-2 D614G Spike.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Benlarbi M, Laroche G, Fink C, Fu K, Mulloy RP, Phan A, Ariana A, 
            Stewart CM, Prevost J, Beaudoin-Bussieres G, Daniel R, Bo Y, El 
            Ferri O, Yockell-Lelievre J, Stanford WL, Giguere PM, Mubareka S, 
            Finzi A, Dekaban GA, Dikeakos JD, Cote M
  TITLE     Identification and differential usage of a host metalloproteinase 
            entry pathway by SARS-CoV-2 Delta and Omicron.
  JOURNAL   iScience. 2022 Nov 18;25(11):105316. doi: 
            10.1016/j.isci.2022.105316. Epub 2022 Oct 10.
  PUBMED    36254158
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-11-18"; volume: "25(11):105316. doi"; pages: " 
            10.1016/j.isci.2022.105316. Epub 2022 Oct 10"
COMMENT     Sequence Label: pCAGGS SARS-CoV-2 D614G Spike
FEATURES             Location/Qualifiers
     source          1..4594
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(3893..3912)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3958..4013
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4012..4031)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4374..4390)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4374..4390)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4387..4409)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4398..4414
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4422..4428,4429..4446,4447..4452))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4467..4488
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 agctcctggg cacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcgccac
       61 catgttcgtc ttcctcgtcc tcctccccct cgtctcctcc cagtgcgtca acctcaccac
      121 ccgcacccag ctcccccccg cctacaccaa ctccttcacc cgcggcgtct actaccccga
      181 caaggtcttc cgctcctccg tcctccactc cacccaggac ctcttcctcc ccttcttctc
      241 caacgtcacc tggttccacg ccatccacgt ctccggcacc aacggcacca agcgcttcga
      301 caaccccgtc ctccccttca acgacggcgt ctacttcgcc tccaccgaga agtccaacat
      361 catccgcggc tggatcttcg gcaccaccct cgactccaag acccagtccc tcctcatcgt
      421 caacaacgcc accaacgtcg tcatcaaggt ctgcgagttc cagttctgca acgacccctt
      481 cctcggcgtc tactaccaca agaacaacaa gtcctggatg gagtccgagt tccgcgtcta
      541 ctcctccgcc aacaactgca ccttcgagta cgtctcccag cccttcctca tggacctcga
      601 gggcaagcag ggcaacttca agaacctccg cgagttcgtc ttcaagaaca tcgacggcta
      661 cttcaagatc tactccaagc acacccccat caacctcgtc cgcgacctcc cccagggctt
      721 ctccgccctc gagcccctcg tcgacctccc catcggcatc aacatcaccc gcttccagac
      781 cctcctcgcc ctccaccgct cctacctcac ccccggcgac tcctcctccg gctggaccgc
      841 cggcgccgcc gcctactacg tcggctacct ccagccccgc accttcctcc tcaagtacaa
      901 cgagaacggc accatcaccg acgccgtcga ctgcgccctc gaccccctct ccgagaccaa
      961 gtgcaccctc aagtccttca ccgtcgagaa gggcatctac cagacctcca acttccgcgt
     1021 ccagcccacc gagtccatcg tccgcttccc caacatcacc aacctctgcc ccttcggcga
     1081 ggtcttcaac gccacccgct tcgcctccgt ctacgcctgg aaccgcaagc gcatctccaa
     1141 ctgcgtcgcc gactactccg tcctctacaa ctccgcctcc ttctccacct tcaagtgcta
     1201 cggcgtctcc cccaccaagc tcaacgacct ctgcttcacc aacgtctacg ccgactcctt
     1261 cgtcatccgc ggcgacgagg tccgccagat cgcccccggc cagaccggca agatcgccga
     1321 ctacaactac aagctccccg acgacttcac cggctgcgtc atcgcctgga actccaacaa
     1381 cctcgactcc aaggtcggcg gcaactacaa ctacctctac cgcctcttcc gcaagtccaa
     1441 cctcaagccc ttcgagcgcg acatctccac cgagatctac caggccggct ccaccccctg
     1501 caacggcgtc gagggcttca actgctactt ccccctccag tcctacggct tccagcccac
     1561 caacggcgtc ggctaccagc cctaccgcgt cgtcgtcctc tccttcgagc tcctccacgc
     1621 ccccgccacc gtctgcggcc ccaagaagtc caccaacctc gtcaagaaca agtgcgtcaa
     1681 cttcaacttc aacggcctca ccggcaccgg cgtcctcacc gagtccaaca agaagttcct
     1741 ccccttccag cagttcggcc gcgacatcgc cgacaccacc gacgccgtcc gcgaccccca
     1801 gaccctcgag atcctcgaca tcaccccctg ctccttcggc ggcgtctccg tcatcacccc
     1861 cggcaccaac acctccaacc aggtcgccgt cctctaccag ggtgtcaact gcaccgaggt
     1921 ccccgtcgcc atccacgccg accagctcac ccccacctgg cgcgtctact ccaccggctc
     1981 caacgtcttc cagacccgcg ccggctgcct catcggcgcc gagcacgtca acaactccta
     2041 cgagtgcgac atccccatcg gcgccggcat ctgcgcctcc taccagaccc agaccaactc
     2101 cccccgccgc gcccgctccg tcgcctccca gtccatcatc gcctacacca tgtccctcgg
     2161 cgccgagaac tccgtcgcct actccaacaa ctccatcgcc atccccacca acttcaccat
     2221 ctccgtcacc accgagatcc tccccgtctc catgaccaag acctccgtcg actgcaccat
     2281 gtacatctgc ggcgactcca ccgagtgctc caacctcctc ctccagtacg gctccttctg
     2341 cacccagctc aaccgcgccc tcaccggcat cgccgtcgag caggacaaga acacccagga
     2401 ggtcttcgcc caggtcaagc agatctacaa gacccccccc atcaaggact tcggcggctt
     2461 caacttctcc cagatcctcc ccgacccctc caagccctcc aagcgctcct tcatcgagga
     2521 cctcctcttc aacaaggtca ccctcgccga cgccggcttc atcaagcagt acggcgactg
     2581 cctcggcgac atcgccgccc gcgacctcat ctgcgcccag aagttcaacg gcctcaccgt
     2641 cctccccccc ctcctcaccg acgagatgat cgcccagtac acctccgccc tcctcgccgg
     2701 caccatcacc tccggctgga ccttcggcgc cggcgccgcc ctccagatcc ccttcgccat
     2761 gcagatggcc taccgcttca acggcatcgg cgtcacccag aacgtcctct acgagaacca
     2821 gaagctcatc gccaaccagt tcaactccgc catcggcaag atccaggact ccctctcctc
     2881 caccgcctcc gccctcggca agctccagga cgtcgtcaac cagaacgccc aggccctcaa
     2941 caccctcgtc aagcagctct cctccaactt cggcgccatc tcctccgtcc tcaacgacat
     3001 cctctcccgc ctcgacaagg tcgaggccga ggtccagatc gaccgcctca tcaccggccg
     3061 cctccagtcc ctccagacct acgtcaccca gcagctcatc cgcgccgccg agatccgcgc
     3121 ctccgccaac ctcgccgcca ccaagatgtc cgagtgcgtc ctcggccagt ccaagcgcgt
     3181 cgacttctgc ggcaagggct accacctcat gtccttcccc cagtccgccc cccacggcgt
     3241 cgtcttcctc cacgtcacct acgtccccgc ccaggagaag aacttcacca ccgcccccgc
     3301 catctgccac gacggcaagg cccacttccc ccgcgagggc gtcttcgtct ccaacggcac
     3361 ccactggttc gtcacccagc gcaacttcta cgagccccag atcatcacca ccgacaacac
     3421 cttcgtctcc ggcaactgcg acgtcgtcat cggcatcgtc aacaacaccg tctacgaccc
     3481 cctccagccc gagctcgact ccttcaagga ggagctcgac aagtacttca agaaccacac
     3541 ctcccccgac gtcgacctcg gcgacatctc cggcatcaac gcctccgtcg tcaacatcca
     3601 gaaggagatc gaccgcctca acgaggtcgc caagaacctc aacgagtccc tcatcgacct
     3661 ccaggagctc ggcaagtacg agcagtacat caagtggccc tggtacatct ggctcggctt
     3721 catcgccggc ctcatcgcca tcgtcatggt caccatcatg ctctgctgca tgacctcctg
     3781 ctgctcctgc ctcaagggct gctgctcctg cggctcctgc tgcaagttcg acgaggacga
     3841 ctccgagccc gtcctcaagg gcgtcaagct ccactacacc taagctagca gatctttttc
     3901 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
     3961 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     4021 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
     4081 caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt
     4141 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
     4201 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
     4261 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
     4321 ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag
     4381 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     4441 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     4501 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat cctcaattag
     4561 tcagcaccat agtcccgccc ctactccgcc atcc
//
