LOCUS       sequence-360345-        4747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant transient expression vector containing mCherry tag. Unique 
            nucleotide sequences Uc and Ud for cloning at N terminus of 
            mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Han J, Ma K, Li H, Su J, Zhou L, Tang J, Zhang S, Hou Y, Chen L, Liu
            YG, Zhu Q
  TITLE     All-in-one: A Robust Fluorescent Fusion Protein Vector Toolbox for 
            Protein Localization and BiFC Analyses in Plants.
  JOURNAL   Plant Biotechnol J. 2022 Feb 18. doi: 10.1111/pbi.13790.
  PUBMED    35179286
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2022 Feb 18. doi: 10.1111/pbi.13790."
COMMENT     Sequence Label: pUC19/N-mChe
FEATURES             Location/Qualifiers
     source          1..4747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        535..879
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     822..842
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    897..921
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             933..953
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1050..1760
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1195..1213)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1463..1482
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      1786..2038
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2095..2112)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2095..2111)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2104..2126)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2320..2339)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2439..2461
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2499..2517)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2585..2689
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2690..2758,2759..3550)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSV
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2908..2927)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3721..4309
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4210..4229
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4463..4480
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4597..4618
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4633..4638,4639..4656,4657..4663)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4671..4687
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4676..4698
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4695..4711
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4695..4711
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ggcgcgccat tactcgcatc cattctcagg ctgtctcgtc tcgtctcaga ttagcctttt
       61 caatttcaga aagaatgcta acccacagat ggttagagag gcttacgcag caggtctcat
      121 caagacgatc tacccgagca ataatctcca ggaaatcaaa taccttccca agaaggttaa
      181 agatgcagtc aaaagattca ggactaactg catcaagaac acagagaaag atatatttct
      241 caagatcaga agtactattc cagtatggac gattcaaggc ttgcttcaca aaccaaggca
      301 agtaatagag attggagtct ctaaaaaggt agttcccact gaatcaaagg ccatggagtc
      361 aaagattcaa atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca
      421 gagtctctta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac
      481 acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac
      541 ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca
      601 ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa
      661 aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
      721 cacgaggagc atcgtggaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
      781 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
      841 tcctctatat aaggaagttc atttcatttg gagagaacac gggggactct agatcaacaa
      901 gtttgtacaa aaaagcaggc tccgcggccg ccttgtttaa ctttaagaag gagcccttca
      961 ccagccaact ccctttacaa cctcactcaa gtccgttaga gcccgggctc gttcgctgcc
     1021 acctaagaat actctacggt cacataccta tggtgagcaa gggcgaggag gataacatgg
     1081 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     1141 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     1201 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     1261 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     1321 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     1381 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     1441 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     1501 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     1561 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     1621 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     1681 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     1741 tggacgagct gtacaagtaa taactcgaga agctcgaatt tccccgatcg ttcaaacatt
     1801 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     1861 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     1921 agatgggttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
     1981 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctaatgtt actagatcgg
     2041 gccaggatac atagattacc acaactccga gcccttccac cggcgcgcca attcactggc
     2101 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     2161 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     2221 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     2281 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     2341 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2401 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2461 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     2521 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     2581 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2641 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2701 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2761 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     2821 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     2881 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     2941 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3001 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3061 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3121 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3181 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     3241 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3301 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggtccttcc
     3361 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3421 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     3481 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3541 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3601 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3661 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3721 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3781 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3841 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3901 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3961 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4021 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4081 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4141 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4201 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4261 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgctagcaa
     4321 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4381 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4441 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     4501 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     4561 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     4621 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     4681 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg catgcctgca
     4741 ggtcccc
//
