LOCUS       sequence-360347-        4346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bimolecular fluorescence complementation (BiFC) vector for transient
            expression of C-truncated Venus in plants. Unique nucleotide 
            sequences Uc and Ud for cloning at N terminus of Vc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Han J, Ma K, Li H, Su J, Zhou L, Tang J, Zhang S, Hou Y, Chen L, Liu
            YG, Zhu Q
  TITLE     All-in-one: A Robust Fluorescent Fusion Protein Vector Toolbox for 
            Protein Localization and BiFC Analyses in Plants.
  JOURNAL   Plant Biotechnol J. 2022 Feb 18. doi: 10.1111/pbi.13790.
  PUBMED    35179286
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2022 Feb 18. doi: 10.1111/pbi.13790."
COMMENT     Sequence Label: pUC19/N-Vc
FEATURES             Location/Qualifiers
     source          1..4346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        536..880
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     823..843
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    898..922
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             934..954
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1057..1083
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1108..1359
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1296..1317
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      1385..1637
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1694..1711)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1694..1710)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1703..1725)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1919..1938)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2038..2060
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2098..2116)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2184..2288
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2289..2357,2358..3149)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSV
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2507..2526)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3320..3908
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3809..3828
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4062..4079
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4196..4217
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4232..4237,4238..4255,4256..4262)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4270..4286
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4275..4297
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4294..4310
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4294..4310
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ggcgcgccgc tgggagttcg tagacggaaa caaacgcaga atccaagcag attagccttt
       61 tcaatttcag aaagaatgct aacccacaga tggttagaga ggcttacgca gcaggtctca
      121 tcaagacgat ctacccgagc aataatctcc aggaaatcaa ataccttccc aagaaggtta
      181 aagatgcagt caaaagattc aggactaact gcatcaagaa cacagagaaa gatatatttc
      241 tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcac aaaccaaggc
      301 aagtaataga gattggagtc tctaaaaagg tagttcccac tgaatcaaag gccatggagt
      361 caaagattca aatagaggac ctaacagaac tcgccgtaaa gactggcgaa cagttcatac
      421 agagtctctt acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca
      481 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga
      541 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc
      601 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata
      661 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
      721 ccacgaggag catcgtggaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
      781 atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc
      841 ttcctctata taaggaagtt catttcattt ggagagaaca cgggggactc tagatcaaca
      901 agtttgtaca aaaaagcagg ctccgcggcc gccttgttta actttaagaa ggagcccttc
      961 accagccaac tccctttaca acctcactca agtccgttag agcccgggct cgttcgctgc
     1021 cacctaagaa tactctacgg tcacatacct atggagtacc catacgacgt accagattac
     1081 gctcatatgg ccatgatcaa gcttgccgac aagcagaaga acggcatcaa ggccaacttc
     1141 aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac
     1201 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
     1261 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1321 gccgccggga tcactctcgg catggacgag ctgtacaagt aactcgagaa gctcgaattt
     1381 ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     1441 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     1501 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     1561 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     1621 atctatgtta ctagatcggg gttccttatc atctggcgaa tcggacccac aagagcactg
     1681 ggcgcgccaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     1741 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     1801 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     1861 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     1921 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     1981 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     2041 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     2101 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     2161 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     2221 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     2281 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     2341 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     2401 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     2461 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     2521 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     2581 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     2641 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     2701 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     2761 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     2821 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     2881 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     2941 ttctgcgctc ggtccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     3001 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     3061 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     3121 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     3181 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     3241 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     3301 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3361 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3421 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3481 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3541 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3601 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3661 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3721 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3781 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3841 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3901 tatggaaaaa cgctagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3961 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     4021 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     4081 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     4141 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     4201 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     4261 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     4321 ccaagcttgc atgcctgcag gtcccc
//
