LOCUS       sequence-360349-        2133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            alpha-factor gene of Kazachstania africana (sender part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK010
FEATURES             Location/Qualifiers
     source          1..2133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(498..532)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(603..1262)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1177..1196
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1444..2032
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1933..1952
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atgaaattta ctggtgtttt gtctgctttg tctttgttat cttctgctgt
       61 tttagctgaa acagaatctc aatctactga agcaatttct gttccatctg aagctattat
      121 tggttattta gatttggatg gtgctaatga tgttgctatt attccattgg ttaatactac
      181 tgattctggt ttgttgtttg ttaatactac tatttatgaa caagcttcta atgaagataa
      241 tgtttcttta aaaaaaagag aagctgacgc tgatgcatgg cactggctat ctattgctcc
      301 aggccaacct atgtatatca agagagatgc aaatgccgat gcggatgcag aagcttggca
      361 ttggttatca atatcaccag gacaaccaat gtacatcaaa agagatgcta atgcagatgc
      421 tgatgctgaa gcttggcatt ggttgtccat tgctccaggt caaccaatgt atatttaagg
      481 atcctgagac cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
      541 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
      601 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
      661 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
      721 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
      781 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata
      841 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
      901 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
      961 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     1021 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
     1081 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     1141 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     1201 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     1261 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     1321 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat
     1381 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1441 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1501 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1561 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1621 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1681 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1741 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1801 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1861 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1921 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1981 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2041 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2101 tgcgttatcc cctgattctg tggataaccg tag
//
