LOCUS       sequence-360350-        9716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant binary vector with ccdB flanked by Ua and Uf unique nucleotide
            sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9716)
  AUTHORS   Han J, Ma K, Li H, Su J, Zhou L, Tang J, Zhang S, Hou Y, Chen L, Liu
            YG, Zhu Q
  TITLE     All-in-one: A Robust Fluorescent Fusion Protein Vector Toolbox for 
            Protein Localization and BiFC Analyses in Plants.
  JOURNAL   Plant Biotechnol J. 2022 Feb 18. doi: 10.1111/pbi.13790.
  PUBMED    35179286
REFERENCE   2  (bases 1 to 9716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2022 Feb 18. doi: 10.1111/pbi.13790."
COMMENT     Sequence Label: pYL1300UaUf
FEATURES             Location/Qualifiers
     source          1..9716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    143..164
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(179..184,185..202,203..209)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    217..233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        275..293
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     275..292
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             367..669
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     548..567
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(810..827)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(810..826)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(819..841)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    1029..1053
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2354..2983
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3412..4485
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4551..4745
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4981..5003)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5089..5229
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5144..5163
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5244..5261)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5415..6003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5495..5514)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6090..6884)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYCILLDELF"
     misc_feature    7309..7333
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    7411..7585
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7626..8651)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(8713..9389)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(8756..8776)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9580..9601
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9616..9621,9622..9639,9640..9646)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9654..9670
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9678..9694
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 attactcgca tccattctca ggctgtctcg tctcgtctcg gcgcgccgta gtgctcgaga
       61 gacctctgaa gtggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      121 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      181 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      241 caagaaacag ctatgaccat gattacgcca agctatttag gtgacactat agaatactca
      301 agctatgcat caagctcaat gggtctagtc tgtagatacc catcacactg gcgaccgctc
      361 gaacatcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
      421 gtacagagtg atattattga cacgccgggg cgacggatgg tgatccccct ggccagtgca
      481 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
      541 agctggcgca tgatgaccac cgatatggcc agtgtgcctg tctccgttat cggggaagaa
      601 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
      661 ggaatataaa tgtcaggcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg
      721 cggcgggtga gcgtgggtct cgcggtatca ttggcgcgcc gttccttatc atctggcgaa
      781 tcggacccac aagagcactg aagcttggca ctggccgtcg ttttacaacg tcgtgactgg
      841 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      901 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      961 gaatgctaga gcagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta
     1021 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     1081 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
     1141 caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt
     1201 gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc
     1261 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt
     1321 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc
     1381 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac
     1441 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc
     1501 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt
     1561 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc
     1621 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc
     1681 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt
     1741 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag
     1801 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac
     1861 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg
     1921 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc
     1981 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa
     2041 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag
     2101 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa
     2161 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg
     2221 ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt
     2281 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac
     2341 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac
     2401 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt
     2461 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct
     2521 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg
     2581 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa
     2641 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc
     2701 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc
     2761 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct
     2821 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg
     2881 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga
     2941 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag
     3001 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag
     3061 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg
     3121 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc
     3181 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag
     3241 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga
     3301 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt
     3361 tgcctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt
     3421 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag
     3481 ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa
     3541 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt
     3601 gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg
     3661 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg
     3721 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta
     3781 gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg
     3841 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc
     3901 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc
     3961 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc
     4021 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc
     4081 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc
     4141 gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg
     4201 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca
     4261 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt
     4321 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat
     4381 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg
     4441 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg
     4501 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc
     4561 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca
     4621 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc
     4681 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt
     4741 gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg
     4801 ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa
     4861 atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc
     4921 gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa
     4981 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     5041 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     5101 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
     5161 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     5221 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5281 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5341 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5401 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5461 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5521 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5581 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5641 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5701 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5761 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5821 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     5881 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5941 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6001 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6061 taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa aatacaatat
     6121 tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt
     6181 tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc
     6241 gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg
     6301 ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt
     6361 aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa
     6421 tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag
     6481 ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca
     6541 tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg
     6601 ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc
     6661 tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca
     6721 taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc
     6781 accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt
     6841 tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct
     6901 cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt
     6961 cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt
     7021 caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg
     7081 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
     7141 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
     7201 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag
     7261 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata
     7321 ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat
     7381 gtactgaatt aacgccgaat taattcgggg gatctggatt ttagtactgg attttggttt
     7441 taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt
     7501 tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct tatctgggaa
     7561 ctactcacac attattatgg agaaactcga gcttgtcgat cgacagatcc cggtcggcat
     7621 ctactctatt tctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
     7681 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
     7741 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
     7801 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac
     7861 gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
     7921 tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa
     7981 tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg
     8041 acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
     8101 caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca
     8161 gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
     8221 tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc
     8281 gcagcgatcg catccatagc ctccgcgacc ggttgtagaa cagcgggcag ttcggtttca
     8341 ggcaggtctt gcaacgtgac accctgtgaa cggcgggaga tgcaataggt caggctctcg
     8401 ctaaactccc caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga
     8461 taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca
     8521 cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg
     8581 tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca
     8641 ggctttttca tatctcattg cccatctgcg aaagctcgag agagatagat ttgtagagag
     8701 agactggtga tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg
     8761 gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca
     8821 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
     8881 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
     8941 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
     9001 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
     9061 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
     9121 gtcgtgctcc accatgttca catcaatcca cttgctttga agacgtggtt ggaacgtctt
     9181 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
     9241 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct
     9301 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc
     9361 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt
     9421 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc
     9481 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     9541 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     9601 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     9661 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattcg agctcc
//
