LOCUS       sequence-360356-        6779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human AKAP79 lacking the calmodulin binding site 
            (Delta-79-86).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Patel N, Stengel F, Aebersold R, Gold MG
  TITLE     Molecular basis of AKAP79 regulation by calmodulin.
  JOURNAL   Nat Commun. 2017 Nov 22;8(1):1681. doi: 10.1038/s41467-017-01715-w.
  PUBMED    29162807
REFERENCE   2  (bases 1 to 6779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-11-22"; volume: "8(1):1681. doi"; pages: " 
            10.1038/s41467-017-01715-w"
COMMENT     Sequence Label: pcDNA3_2FLAG-AKAP79-Delta-CaM
FEATURES             Location/Qualifiers
     source          1..6779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..41)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(195..783)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(275..294)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(954..1745,1746..1814))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1577..1596
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1815..1919)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1994..2013)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2185..2564
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2565..2768
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2719..2739
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2813..2832
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2813..2831
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2883..2930
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             4227..4268
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4278..4295
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4318..4335)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4324..4548
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4594..5022
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4681..4700)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4891..4912
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5031..5051)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5036..5365
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5216..5351
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5278..5297
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5432..6226
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5486..5505)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6096..6115
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6402..6523
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6439..6458)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6493..6512
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6572..6588)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6572..6588)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6585..6607)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6596..6612
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6620..6626,6627..6644,6645..6650))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6665..6686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
       61 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      121 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      181 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      241 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      301 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      361 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      421 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      481 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      541 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      601 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
      661 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
      721 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
      781 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
      841 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
      901 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
      961 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1021 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1081 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1141 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1201 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1261 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1321 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1381 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     1441 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1501 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1561 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     1621 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     1681 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     1741 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     1801 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     1861 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     1921 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     1981 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     2041 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     2101 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     2161 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     2221 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     2281 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2341 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2401 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2461 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2521 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2581 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2641 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2701 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2761 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
     2821 ctcactatag ggagacccaa gctggctagt taagcttggt accgagctcg gatcccacca
     2881 tggactataa ggatgacgat gacaaagact acaaagatga tgacgataaa atggaaacca
     2941 caatttcaga aattcatgta gaaaacaagg atgagaagag atcagcagaa ggtagtcctg
     3001 gggctgaaag gcagaaggaa aaggcatcca tgctttgctt caagagaaga aagaaagcag
     3061 ccaaagcact gaagcccaaa gctggctctg aagctgctga tgtggcaagg aagtgtccac
     3121 aagaagcagg agcttctgat cagccagagc ccacacgggg ggccacacgc aggaaaaggt
     3181 cagagtcttc aaagcagcaa aagccattgg agggtgaaat gcaacctgca ataaatgctg
     3241 aggatgctga tctttctaag aaaaaggcaa aatctagact taagattccc tgcataaaat
     3301 tcccaagagg gccaaaaagg agtaatcatt ccaaaattat agaagactca gactgcagca
     3361 tcaaagtcca ggaagaagct gaaattttgg atatacaaac acagacccca ttgaatgatc
     3421 aggcaacaaa ggctaagtca acccaggatc taagtgaagg catctcacag aaagatggtg
     3481 atgaggtctg tgaatcaaat gtgagcaata gcataacttc tggagagaaa gtgatttcag
     3541 tagaacttgg attagataat gggcattctg ctattcaaac gggaactcta atccttgaag
     3601 aaattgaaac gatcaaggaa aaacaagatg ttcaacccca gcaagcaagc ccacttgaaa
     3661 cttcagaaac agaccatcag cagccagtac tttctgatgt tcctccttta cctgcaattc
     3721 cagatcaaca aattgtggaa gaagccagta acagtaccct agaaagtgca ccaaatggaa
     3781 aagactatga aagtacagag attgtagctg aagaaactaa gccaaaagat actgaattga
     3841 gccaagaatc agattttaaa gaaaatggga tcactgaaga gaaatccaaa tcagaagaaa
     3901 gcaaaagaat ggagccaatt gctattatta ttacagacac tgaaatcagt gaatttgatg
     3961 ttacaaaatc taaaaatgtc cctaagcaat tcttaatttc agctgaaaat gagcaagtag
     4021 gggtttttgc taatgataat ggttttgagg atagaacttc agaacaatat gaaacactct
     4081 taattgaaac agcctcttct ctagtcaaga atgctattca gttgtcaata gaacagctgg
     4141 ttaatgaaat ggcctctgat gataataaaa taaacaatct tctacagtga ggcggccgct
     4201 cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg
     4261 attctacgcg taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct
     4321 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     4381 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     4441 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     4501 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     4561 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     4621 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4681 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4741 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4801 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4861 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4921 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     4981 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     5041 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5101 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5161 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     5221 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5281 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     5341 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     5401 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     5461 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     5521 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     5581 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     5641 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     5701 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     5761 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     5821 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     5881 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     5941 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     6001 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     6061 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     6121 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     6181 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     6241 ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     6301 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     6361 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     6421 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6481 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     6541 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6601 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6661 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6721 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttg
//
