LOCUS       sequence-360363-       11236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Replacement of endogenous rat PKA-RII-alpha subunits with wild-type 
            RII-alpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11236)
  AUTHORS   Church TW, Tewatia P, Hannan S, Antunes J, Eriksson O, Smart TG, 
            Hellgren Kotaleski J, Gold MG
  TITLE     AKAP79 enables calcineurin to directly suppress protein kinase A 
            activity.
  JOURNAL   Elife. 2021 Oct 6;10. pii: 68164. doi: 10.7554/eLife.68164.
  PUBMED    34612814
REFERENCE   2  (bases 1 to 11236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-10-6"; volume: "10. pii: 68164. doi"; pages: " 
            10.7554/eLife.68164"
COMMENT     Sequence Label: pFUGW-shRII-alpha-RII-alpha-WT-IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..11236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1262..1661)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     promoter        1690..1904
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     1825..1844
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_feature    2052..2169
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2467..2511)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2696..2929
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3422..3547
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3594..3774
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3792..3990)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3817..3837)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3992..4371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4543..4562
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4637..4741
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4742..4810,4811..5602)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4960..4979)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5773..6361
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6262..6281
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6515..6532
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6649..6670
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6685..6690,6691..6708,6709..6715)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6723..6739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6728..6750
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6747..6763
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6747..6763
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6812..6933
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6823..6842)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6877..6896
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7063..7437)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7456..7503)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7551..7880)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7565..7700
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7619..7638)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7865..7885
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7894..8322)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8004..8025)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8216..8235
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8368..8592
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8581..8598
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             8615..8795
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     9300..9316
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     9301..9317
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    9320..9908
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             9426..9437
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9835..9855
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(9935..10648,10649..10651,10652..10654))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(9938..10648,10649..10651,10652..10654))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9980..10001)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     10329..10348
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10588..10609
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(10655..10663,10664..10666,10667..11236,1..5)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10828..10847)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     11057..11074
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 aggggcggat ccgagctact gcccggggtc caatagatcc aagttggagc caaacatctt
       61 caccagctgt tcttcgtaat gtgagatgtt cctcttcatg atgtccatgc aggggcccag
      121 aagcctctca aatgcttgaa catccatgac taagcatttg acgtctccaa ccgcataagc
      181 agaagcagct cttggtttgt tggtcaccag ggcaagttct ccaaagtact gccccttatg
      241 gcagtgggca atctcaacct cctggttccc gccgttcttg ttcgttttag tcttgcttct
      301 aatcaagatg ctcacttctc cagactctat aatataaaag ctgtcggcct tttcaccctg
      361 agtgattatt cgctctccat ccttatagat cttttccccg atcacatcca caatcttcat
      421 tcgttctgac atctctagtg atttaaagag cggtacagac tcaataaacg attcgaacat
      481 cttcctcttc tttgcattgt tcttcactat gattctccta aaagtcaccc ggtccaatcc
      541 ccaaagggag ccgtctgagg tggccacaat ggtagcagct ctcggggtat tgtacatcag
      601 ggctagttct ccaaaactgc cacggttgtc atactgacca acagatcgtg tttgattatc
      661 ttttgttact aaaatgtcat aggttcccct ttctatgaca taaaagttgt ctccatcatc
      721 tccttggtca atgacatgct cgtcagtttt gactatcttt tcaaacatgg cgtccagaac
      781 ttgagaaagc tgttcctgat ccaggttttt gaaaagcaga atgtctttac aggcttcctg
      841 aagtctgcac ctctgctcgt cggttttggg gtgaaccacc cttggatcat tatcttcttc
      901 ttcatcaggg ttaaacgttt ctgcacagac tgatactcgt ctagtaaatt tgctgggaat
      961 cggaacttcc agatcctcat cgtccgagtc cgattcactc tccccgtcct cctcgatgac
     1021 ggggaccccg ctggactcct gcgcgtgaaa ggtcgtcggg ggggcgatga acgagtctga
     1081 ctcttgccgg cgggcctcgc gcaggcgtgt gaagtactcc accgcgaagt cgacgaggtc
     1141 gggcggctgc tgccgaagca cctccacggt gtagccctgc agcagctccg tgagccccgg
     1201 tgggatctgg atgtggctca tggtggaatt cgaagcttgc tagcccagca gcccgctact
     1261 caccaagtga cgatcacagc gatccacaaa caagaaccgc gacccaaatc ccggctgcga
     1321 cggaactagc tgtgccacac ccggcgcgtc cttatataat catcggcgtt caccgcccca
     1381 cggagatccc tccgcagaat cgccgagaag ggactacttt tcctcgcctg ttccgctctc
     1441 tggaaagaaa accagtgccc tagagtcacc caagtcccgt cctaaaatgt ccttctgctg
     1501 atactggggt tctaaggccg agtcttatga gcagcgggcc gctgtcctga gcgtccgggc
     1561 ggaaggatca ggacgctcgc tgcgcccttc gtctgacgtg gcagcgctcg ccgtgaggag
     1621 gggggcgccc gcgggaggcg ccaaaacccg gcgcggaggc cagatctgga gccgacacgg
     1681 gttaattaag aacgctgacg tcatcaaccc gctccaagga atcgcgggcc cagtgtcact
     1741 aggcgggaac acccagcgcg cgtgcgccct ggcaggaaga tggctgtgag ggacagggga
     1801 gtggcgccct gcaatatttg catgtcgcta tgtgttctgg gaaatcacca taaacgtgaa
     1861 atgtctttgg atttgggaat cttataagtt ctgtatgaga ccactctttc ccgccaggaa
     1921 tcagactcgt tcattcaaga gatgaacgag tctgattcct ggcttttttg tctagagatc
     1981 taagggcgaa ttctttcgga tcctttctta attaaccaaa ctggatctct gctgtccctg
     2041 taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg
     2101 tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt
     2161 cttttaaaat tgtggatgaa tactgccatt tgtctgcaga attggcgcac gcagtgccga
     2221 tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc
     2281 ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata
     2341 tccctgccta actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa
     2401 gcctcctact atcattatga ataattttat ataccacagc caatttgtta tgttaaacca
     2461 attccacaaa cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg
     2521 gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc
     2581 ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc
     2641 attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag
     2701 ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc
     2761 ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg
     2821 ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc
     2881 gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc
     2941 tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc
     3001 aatttttact actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc
     3061 tccaggtctg aagatcagcg gccgcttgct gtgcggtggt cttacttttg ttttgctctt
     3121 cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat
     3181 agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg
     3241 gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa
     3301 ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct
     3361 ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac
     3421 tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac
     3481 tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc
     3541 gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga
     3601 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
     3661 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
     3721 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca
     3781 ggcaaaacgc gctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
     3841 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac
     3901 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
     3961 gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga
     4021 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
     4081 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
     4141 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
     4201 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
     4261 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
     4321 ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt caacgcgtat
     4381 atctggcccg tacatcgcga agcagcgcaa aacgcctaac cctaagcaga ttcttcatgc
     4441 aattgtcggt caagccttgc cttgttgtag cttaaatttt gctcgcgcac tactcagcga
     4501 cctccaacac acaagcaggg agcagatact ggcttaacta tgcggcatca gagcagattg
     4561 tactgagagt gcaccatagg ggatcgggag atctcccgat ccgtcgacgt caggtggcac
     4621 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4681 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4741 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4801 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4861 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4921 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4981 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5041 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5101 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5161 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5221 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5281 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5341 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5401 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5461 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5521 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5581 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5641 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5701 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5761 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5821 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5881 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5941 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6001 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6061 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6121 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6181 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6241 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6301 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6361 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6421 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6481 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6541 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     6601 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     6661 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     6721 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     6781 ctagctagag gtcgacggta tacagacatg ataagataca ttgatgagtt tggacaaacc
     6841 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     6901 tttgtaacca ttataagctg caataaacaa gttggggtgg gcgaagaact ccagcatgag
     6961 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     7021 ttcatagaag gcggcggtgg aatcgaaatc tcgtagcacg tgtcagtcct gctcctcggc
     7081 cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg aactcccgcc cccacggctg
     7141 ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc
     7201 cgaccactcg gcgtacagct cgtccaggcc gcgcacccac acccaggcca gggtgttgtc
     7261 cggcaccacc tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc
     7321 ggcgaagtcg tcctccacga agtcccggga gaacccgagc cggtcggtcc agaactcgac
     7381 cgctccggcg acgtcgcgcg cggtgagcac cggaacggca ctggtcaact tggccatggt
     7441 ttagttcctc accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc
     7501 aacacgtgct gatcagatcc gaaaatggat atacaagctc ccgggagctt tttgcaaaag
     7561 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc agaggcggcc
     7621 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     7681 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     7741 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt
     7801 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     7861 ccacacccta actgacacac attccacaga attaattcgc gttaaatttt tgttaaatca
     7921 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     7981 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     8041 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     8101 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     8161 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     8221 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     8281 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtggggatac cccctagagc
     8341 cccagctggt tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc
     8401 tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat
     8461 gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag
     8521 tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac
     8581 tagaaggcac agtcgaggct gatcagcggg tttctgctag agattttcca cactgactaa
     8641 aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga
     8701 agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc
     8761 tcccaggctc agatctggtc taaccagaga gacccagtac agtccggatg cagctctcgg
     8821 gccatgtgat gaaatgctag gcggctgtca aacctccact ctaatacttc tctctccggg
     8881 tcatccatcc catgcaggct cacagggtgt aacaagcggg tgttctctcc ttcattggct
     8941 tcttctacct tctcttgctc aactggtact agcttgtagc accatccaaa ggtcagtgga
     9001 tatctgatcc ctggccctgg tgtgtagttc tgccaatcag ggaagtagcc ttgtgtgtgg
     9061 tagatccaca gatcaaggat atcttgtctt cgttgggagt gaattagccc ttccagtccc
     9121 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
     9181 ggtacctgag gtgtgactgg aaaacccacc tcctcctcct cttgtgcttc tagccaggca
     9241 caatcagcat tggtagctgc tgtattgcta cttgtgattg ctccatgttt ttctaggtct
     9301 cgaggtcgac ggtatcgatg cggggaggcg gcccaaaggg agatccgact cgtctgaggg
     9361 cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc
     9421 gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca
     9481 acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac ggaattgtca
     9541 gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc
     9601 gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg
     9661 ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca
     9721 acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg
     9781 aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc
     9841 gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga
     9901 ggttgattat cgataagctt gatatcgaat tgctttactt gtacagctcg tccatgccga
     9961 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg
    10021 ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc
    10081 cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga
    10141 tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat
    10201 agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
    10261 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
    10321 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc
    10381 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga
    10441 agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
    10501 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
    10561 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
    10621 ccccggtgaa cagctcctcg cccttgctca ccatggttgt ggccatatta tcatcgtgtt
    10681 tttcaaagga aaaccacgtc cccgtggttc ggggggccta gacgtttttt taacctcgac
    10741 taaacacatg taaagcatgt gtaccgaggc cccagatcag atcccataca atggggtacc
    10801 ttctgggcat ccttcagccc cttgttgaat acgcttgagg agagccattt gactctttcc
    10861 acaactatcc aactcacaac gtggcactgg ggttgtgccg cctttgcagg tgtatcttat
    10921 acacgtggct tttggccgca gaggcacctg tcgccaggtg gggggttccg ctgcctgcaa
    10981 agggtcgcta cagacgttgt ttgtcttcaa gaagcttcca gaggaactgc ttccttcacg
    11041 acattcaaca gaccttgcat tcctttggcg agaggggaaa gacccctagg aatgctcgtc
    11101 aagaagacag ggccaggttt ccgggccctc acattgccaa aagacggcaa tatggtggaa
    11161 aataacatat agacaaacgc acaccggcct tattccaagc ggcttcggcc agtaacgtta
    11221 gggggggggg agggag
//
