LOCUS       sequence-360367-       12112 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Replacement of endogenous rat PKA-RII-alpha subunits with the 
            RII-alpha S97E variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12112)
  AUTHORS   Church TW, Tewatia P, Hannan S, Antunes J, Eriksson O, Smart TG, 
            Hellgren Kotaleski J, Gold MG
  TITLE     AKAP79 enables calcineurin to directly suppress protein kinase A 
            activity.
  JOURNAL   Elife. 2021 Oct 6;10. pii: 68164. doi: 10.7554/eLife.68164.
  PUBMED    34612814
REFERENCE   2  (bases 1 to 12112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-10-6"; volume: "10. pii: 68164. doi"; pages: " 
            10.7554/eLife.68164"
COMMENT     Sequence Label: pFUGW-shRII-alpha-RII-alpha-S97E-IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..12112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(45..65)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        50..379
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      230..365
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     292..311
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        427..474
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             493..867
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    997..1118
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1034..1053)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1088..1107
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1167..1183)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1167..1183)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1180..1202)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1191..1207
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1215..1221,1222..1239,1240..1245))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1260..1281
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1398..1415)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1569..2157)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1649..1668)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2328..3119,3120..3188))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2951..2970
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3189..3293)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3368..3387)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3559..3938
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3940..4138
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4093..4113
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4156..4336
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4383..4508
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5001..5234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5419..5463
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5761..5878
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(6026..6240)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(6086..6105)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        6269..6668
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     misc_feature    join(7925..8499,8500..8502,8503..8511)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8092..8109)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8319..8338
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8512..8514,8515..8517,8518..9231)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8512..8514,8515..8517,8518..9228)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      9382..9391
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9388..9390,9391..9393,9394..10107)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9388..9390,9391..9393,9394..10104)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    10134..10722
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10187..10207)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10605..10616)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10725..10741)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10726..10742)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11247..11427
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11444..11461)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11450..11674
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(11720..12112,1..36)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11807..11826)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12017..12038
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
       61 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      121 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      181 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      241 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      301 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
      361 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
      421 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
      481 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
      541 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
      601 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
      661 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
      721 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
      781 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
      841 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
      901 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
      961 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     1021 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     1081 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     1141 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     1201 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     1261 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     1321 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     1381 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1441 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1501 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1561 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1621 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1681 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1741 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1801 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1861 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1921 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1981 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     2041 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2101 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2161 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2221 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2281 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2341 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2401 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2461 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2521 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2581 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2641 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2701 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2761 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2821 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2881 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2941 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     3001 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     3061 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     3121 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     3181 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3241 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3301 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     3361 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     3421 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     3481 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     3541 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     3601 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     3661 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     3721 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     3781 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     3841 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     3901 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     3961 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     4021 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     4081 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg
     4141 cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     4201 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     4261 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     4321 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     4381 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     4441 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     4501 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     4561 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     4621 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     4681 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     4741 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     4801 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     4861 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     4921 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     4981 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     5041 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     5101 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     5161 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     5221 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     5281 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     5341 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     5401 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     5461 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     5521 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     5581 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     5641 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     5701 gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa
     5761 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     5821 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     5881 ggtttattac agggacagca gagatccagt ttggttaatt aagaaaggat ccgaaagaat
     5941 tcgcccttag atctctagac aaaaaagcca ggaatcagac tcgttcatct cttgaatgaa
     6001 cgagtctgat tcctggcggg aaagagtggt ctcatacaga acttataaga ttcccaaatc
     6061 caaagacatt tcacgtttat ggtgatttcc cagaacacat agcgacatgc aaatattgca
     6121 gggcgccact cccctgtccc tcacagccat cttcctgcca gggcgcacgc gcgctgggtg
     6181 ttcccgccta gtgacactgg gcccgcgatt ccttggagcg ggttgatgac gtcagcgttc
     6241 ttaattaacc cgtgtcggct ccagatctgg cctccgcgcc gggttttggc gcctcccgcg
     6301 ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct
     6361 gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa
     6421 ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt
     6481 ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag
     6541 ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag
     6601 ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt
     6661 cacttggtga gtagcgggct gctgggctag caagcttcga attccaccat gagccacatc
     6721 cagatcccac cggggctcac ggagctgctg cagggctaca ccgtggaggt gcttcggcag
     6781 cagccgcccg acctcgtcga cttcgcggtg gagtacttca cacgcctgcg cgaggcccgc
     6841 cggcaagagt cagactcgtt catcgccccc ccgacgacct ttcacgcgca ggagtccagc
     6901 ggggtccccg tcatcgagga ggacggggag agtgaatcgg actcggacga tgaggatctg
     6961 gaagttccga ttcccagcaa atttactaga cgagtagaag tctgtgcaga aacgtttaac
     7021 cctgatgaag aagaagataa tgatccaagg gtggttcacc ccaaaaccga cgagcagagg
     7081 tgcagacttc aggaagcctg taaagacatt ctgcttttca aaaacctgga tcaggaacag
     7141 ctttctcaag ttctggacgc catgtttgaa aagatagtca aaactgacga gcatgtcatt
     7201 gaccaaggag atgatggaga caacttttat gtcatagaaa ggggaaccta tgacatttta
     7261 gtaacaaaag ataatcaaac acgatctgtt ggtcagtatg acaaccgtgg cagttttgga
     7321 gaactagccc tgatgtacaa taccccgaga gctgctacca ttgtggccac ctcagacggc
     7381 tccctttggg gattggaccg ggtgactttt aggagaatca tagtgaagaa caatgcaaag
     7441 aagaggaaga tgttcgaatc gtttattgag tctgtaccgc tctttaaatc actagagatg
     7501 tcagaacgaa tgaagattgt ggatgtgatc ggggaaaaga tctataagga tggagagcga
     7561 ataatcactc agggtgaaaa ggccgacagc ttttatatta tagagtctgg agaagtgagc
     7621 atcttgatta gaagcaagac taaaacgaac aagaacggcg ggaaccagga ggttgagatt
     7681 gcccactgcc ataaggggca gtactttgga gaacttgccc tggtgaccaa caaaccaaga
     7741 gctgcttctg cttatgcggt tggagacgtc aaatgcttag tcatggatgt tcaagcattt
     7801 gagaggcttc tgggcccctg catggacatc atgaagagga acatctcaca ttacgaagaa
     7861 cagctggtga agatgtttgg ctccaacttg gatctattgg accccgggca gtagctcgga
     7921 tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     7981 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     8041 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     8101 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     8161 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     8221 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     8281 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     8341 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     8401 ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     8461 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc
     8521 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     8581 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     8641 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     8701 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     8761 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     8821 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     8881 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     8941 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     9001 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     9061 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     9121 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     9181 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc
     9241 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     9301 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     9361 ttattgcagc ttataccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     9421 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     9481 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     9541 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     9601 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     9661 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     9721 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     9781 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     9841 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     9901 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     9961 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
    10021 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
    10081 ctcggcatgg acgagctgta caagtaaagc aattcgatat caagcttatc gataatcaac
    10141 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
    10201 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
    10261 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
    10321 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
    10381 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
    10441 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
    10501 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
    10561 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
    10621 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
    10681 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
    10741 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
    10801 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
    10861 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
    10921 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
    10981 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
    11041 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
    11101 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
    11161 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
    11221 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
    11281 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
    11341 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
    11401 tttagtcagt gtggaaaatc tctagcagaa acccgctgat cagcctcgac tgtgccttct
    11461 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
    11521 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
    11581 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
    11641 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
    11701 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
    11761 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
    11821 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
    11881 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
    11941 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
    12001 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    12061 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aa
//
