LOCUS       sequence-360388-        9681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant 
            bacterial expression and purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang 
            F
  TITLE     Multiplexed and portable nucleic acid detection platform with Cas13,
            Cas12a, and Csm6.
  JOURNAL   Science. 2018 Apr 27;360(6387):439-444. doi: 
            10.1126/science.aaq0179. Epub 2018 Feb 15.
  PUBMED    29449508
REFERENCE   2  (bases 1 to 9681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaq0179"; journalName: "Science"; date: 
            "2018-04-27- 27"; volume: "360"; issue: "6387"; pages: "439-444"
COMMENT     Sequence Label: pR0306 CcaCas13b His6-TwinStrep-SUMO
FEATURES             Location/Qualifiers
     source          1..9681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1060..2142)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     2104..2123
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(2143..2220)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     2426..2445
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2529..2547
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2549..2572
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2587..2609
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2628..2645
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2655..2672
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(2685..2708,2709..2744,2745..2768)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             2769..3062
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     primer_bind     complement(6710..6728)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6724..6771
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(6946..6951,6952..6968,6969..6974))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(7001..7019)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7087..7191
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7192..7260,7261..8052)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7410..7429)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8223..8811
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8712..8731
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8965..8982
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8997..9136
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9062..9081)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9222..9244
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(9238..9429)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa
       61 gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg
      121 ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt
      181 cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat
      241 gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt
      301 ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc
      361 gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga
      421 cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc
      481 catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccaat gatcgaagtt
      541 aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc
      601 ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata
      661 atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg
      721 gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg
      781 acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc
      841 gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt
      901 cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc
      961 gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt
     1021 gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg
     1081 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     1141 cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc
     1201 cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag
     1261 gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc
     1321 gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg
     1381 cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc
     1441 attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc
     1501 cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg
     1561 cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac
     1621 cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg
     1681 tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc
     1741 aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag
     1801 aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac
     1861 cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc
     1921 gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg
     1981 ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg
     2041 cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac
     2101 accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg
     2161 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc
     2221 cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
     2281 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
     2341 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     2401 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     2461 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
     2521 cgcgaaatta atacgactca ctataggtga attgtgagcg gataacaatt cccctctaga
     2581 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
     2641 atcacagcag cggcctggtg ccgcgcggca gccatatggc tagctggagc catccgcagt
     2701 ttgaaaaagg tggtggtagc ggtggtggtt caggtggtag tgcatggtca caccctcagt
     2761 ttgagaaaat gtcggactca gaagtcaatc aagaagctaa gccagaggtc aagccagaag
     2821 tcaagcctga gactcacatc aatttaaagg tgtccgatgg atcttcagag atcttcttca
     2881 agatcaaaaa gaccactcct ttaagaaggc tgatggaagc gttcgctaaa agacagggta
     2941 aggaaatgga ctccttaaga ttcttgtacg acggtattag aatccaagct gatcagaccc
     3001 ctgaagattt ggacatggag gataacgata ttattgaggc tcacagagaa cagattggtg
     3061 gatctatgaa gaacatccag cggctcggca agggcaacga gttcagcccc ttcaagaaag
     3121 aggacaagtt ctacttcggc ggcttcctga acctggccaa caacaacatc gaggatttct
     3181 tcaaagagat catcacccgc ttcggcatcg tgatcaccga cgagaacaag aagcccaaag
     3241 agacattcgg cgagaagatc ctgaacgaga tcttcaaaaa ggacatcagc atcgtggact
     3301 acgagaaatg ggtcaacatc ttcgccgact acttcccgtt caccaagtac ctgagcctgt
     3361 acctggaaga gatgcagttc aagaaccgcg tgatctgctt ccgggacgtg atgaaggaac
     3421 tgctgaaaac cgtggaagcc ctgcggaact tctacaccca ctacgaccac gagcctatca
     3481 agatcgagga ccgggtgttc tactttctgg acaaggtgct gctggacgtg tccctgaccg
     3541 tgaagaacaa gtacctcaag accgacaaga ccaaagagtt cctcaaccag cacatcggcg
     3601 aggaactgaa agagctgtgc aagcagcgga aggactacct cgtcggaaag ggcaagagaa
     3661 tcgacaaaga gagcgagatc attaacggca tctacaacaa cgccttcaag gacttcatct
     3721 gcaagcgcga gaagcaggac gacaaagaaa accacaacag cgtggaaaag atcctgtgca
     3781 acaaagagcc ccagaacaaa aagcagaaaa gcagcgccac cgtgtgggag ctgtgtagca
     3841 agagcagcag caagtacacc gaaaagagct tccccaaccg cgagaacgac aagcactgcc
     3901 tggaagtgcc catcagccag aaaggcatcg tgtttctgct gtcattcttt ctgaacaaag
     3961 gcgagatcta cgccctgacc agcaacatca agggcttcaa ggccaagatc accaaagagg
     4021 aacccgtcac ctacgacaag aacagcatcc ggtacatggc cacacaccgg atgttcagtt
     4081 ttctggccta caagggcctg aagcggaaga tcagaacctc cgagatcaac tacaacgagg
     4141 acggccaggc cagctccaca tacgagaaag aaaccctgat gctgcagatg ctggacgagc
     4201 tgaacaaggt gcccgacgtg gtgtaccaga acctgagcga ggacgtgcag aaaaccttca
     4261 tcgaggactg gaacgagtac ctgaaagaaa acaacggcga cgtgggcacc atggaagagg
     4321 aacaagtgat tcaccccgtg atccggaaga gatacgagga taagttcaac tacttcgcca
     4381 tccggtttct ggacgagttc gcccagtttc ctacactgcg gttccaggtc cacctgggca
     4441 actacctgtg cgacaagcgg accaagcaga tctgtgacac caccaccgag cgggaagtga
     4501 agaaaaagat caccgtgttc ggccggctga gcgagctgga aaacaagaag gccatcttcc
     4561 tcaacgagcg cgaggaaatc aaaggctggg aagtgttccc caatcctagc tacgacttcc
     4621 ccaaagaaaa catcagcgtg aactacaagg actttcccat cgtgggcagc atcctggacc
     4681 gggaaaaaca gcccgtgtcc aacaagatcg gcatcagagt gaagatcgcc gacgagctgc
     4741 agagagagat cgataaggcc atcaaagaga agaagctgcg gaaccccaag aaccggaagg
     4801 ccaaccagga tgagaagcag aaagagcggc tggtcaacga gatcgtgtcc accaacagca
     4861 acgagcaggg cgagcctgtg gtgtttatcg gacagcctac agcctacctg agcatgaacg
     4921 acatccacag cgtgctgtac gagttcctga tcaacaagat tagcggcgag gccctggaaa
     4981 caaagatcgt cgagaagatt gagacacaga tcaagcagat tatcggcaag gacgccacca
     5041 ccaagattct gaagccctac accaacgcca actccaacag catcaacagg gaaaagctgc
     5101 tgcgcgatct ggaacaagag caacagatcc tcaagacgct gctggaagaa caacagcagc
     5161 gcgaaaagga caagaaggat aagaagtcca agcggaagca cgagctgtac cccagcgaga
     5221 agggaaaagt ggctgtgtgg ctggccaacg acatcaagcg gttcatgccc aaggccttta
     5281 aagagcagtg gcggggctac caccacagcc tgctgcagaa gtacctggct tactacgagc
     5341 agagcaaaga agaactgaag aacctgctgc cgaaagaggt gttcaaacac tttccgttca
     5401 agctgaaggg ctactttcag cagcagtatc tgaaccagtt ctacacggac tacctgaaga
     5461 ggcggctgag ctacgtgaac gagctgctcc tgaatatcca gaacttcaag aacgataagg
     5521 acgccctgaa ggccaccgag aaagagtgct tcaagttctt ccgcaagcag aactacatca
     5581 tcaaccccat caacatccag atccagtcca tcctggtgta ccctatcttc ctgaagagag
     5641 gcttcctgga cgagaagccc accatgatcg acagagagaa gtttaaagag aacaaggaca
     5701 ccgagctggc cgactggttc atgcactaca agaattacaa agaggataac taccagaagt
     5761 tctacgccta tccgctggaa aaggtggaag agaaagagaa gttcaaacgc aacaagcaaa
     5821 tcaacaaaca gaagaagaac gacgtctaca ccctgatgat ggtcgagtac atcatccaga
     5881 agatctttgg cgacaagttc gtcgaagaga accctctggt gctgaaggga atcttccaga
     5941 gcaaggccga gcggcagcag aacaataccc atgccgccac aacacaagag cggaacctga
     6001 acggcatcct gaatcagccc aaggacatta agatccaggg caagatcaca gtgaagggcg
     6061 tgaagctgaa agacatcggc aatttccgga agtacgagat cgaccagcgc gtgaacacct
     6121 tcctggatta cgagccccgg aaagaatgga tggcctacct gcctaacgac tggaaagaga
     6181 aagaaaagca gggacagctg cctccgaaca acgtgatcga ccggcagatc agcaaatacg
     6241 agacagtgcg gagcaagatc ctgctgaagg atgtgcaaga gcttgagaag atcatcagcg
     6301 acgagatcaa agaggaacac cggcacgacc tgaagcaggg caagtactac aacttcaagt
     6361 actatatcct caacggcctg ctgcggcagc tgaagaacga gaacgtggaa aattacaagg
     6421 ttttcaagct caacacgaac cccgagaaag tgaacatcac ccagctgaag caagaggcca
     6481 cagacctcga acagaaagcc ttcgtgctga cctacatccg gaacaagttt gcccacaacc
     6541 agctgcctaa gaaagagttc tgggactact gccaagagaa gtacgggaag atcgaaaaag
     6601 agaaaaccta cgccgagtac ttcgccgaag tgttcaagcg ggaaaaagag gccctgatca
     6661 agtaaggccg cactcgaggc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     6721 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
     6781 ggaactatat ccggatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc
     6841 ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac
     6901 acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga
     6961 tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt
     7021 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     7081 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7141 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     7201 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     7261 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     7321 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     7381 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     7441 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     7501 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     7561 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     7621 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     7681 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     7741 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     7801 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     7861 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc acgcggtatc
     7921 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     7981 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8041 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8101 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     8161 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8221 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     8281 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     8341 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     8401 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     8461 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     8521 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8581 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     8641 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     8701 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     8761 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8821 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8881 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8941 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     9001 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg tgcactctca
     9061 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     9121 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     9181 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     9241 gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg
     9301 gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc
     9361 cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg
     9421 tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat
     9481 gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga
     9541 acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca
     9601 gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca
     9661 tcctgcgatg cagatccgga a
//
