LOCUS       sequence-360396-       12898 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAVS1 safe-harbor integration of dox-inducible NFIA-SOX9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12898)
  AUTHORS   Leng K, Rose IVL, Kim H, Xia W, Romero-Fernandez W, Rooney B, Koontz
            M, Li E, Ao Y, Wang S, Krawczyk M, Tcw J, Goate A, Zhang Y, Ullian 
            EM, Sofroniew MV, Fancy SPJ, Schrag MS, Lippmann ES, Kampmann M
  TITLE     CRISPRi screens in human iPSC-derived astrocytes elucidate 
            regulators of distinct inflammatory reactive states.
  JOURNAL   Nat Neurosci. 2022 Nov;25(11):1528-1542. doi: 
            10.1038/s41593-022-01180-9. Epub 2022 Oct 27.
  PUBMED    36303069
REFERENCE   2  (bases 1 to 12898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-022-01180-9"; journalName: "Nat Neurosci"; date: 
            "2022-11"; volume: "25"; issue: "11"; pages: "1528-1542"
COMMENT     Sequence Label: pKL100
FEATURES             Location/Qualifiers
     source          1..12898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(54..122,123..914)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(272..291)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1085..1673
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1574..1593
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    1721..2557
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    2590..2608
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2626..2644
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2662..2680
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2698..2716
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2734..2752
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2770..2788
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2806..2824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2869..2893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4512..4565
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    6112..6233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6149..6168)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6203..6222
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6282..6298)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6282..6298)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6295..6317)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6306..6322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6330..6336,6337..6354,6355..6360))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6375..6396
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6513..6530)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     6822..6841
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    6840..6895
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     6941..6960
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(7050..7796)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7828..7847)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          7855..8872
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(7909..7931)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(8874..9151)
                     /label=chicken beta-actin promoter
     enhancer        9153..9532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    9538..9762
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     9751..9768
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    9784..9817
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(10093..10112)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     10362..10380
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      10522..10531
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             10569..10837
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(10850..11061)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     polyA_signal    11097..11321
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(11342..11941)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11425..11445)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     11922..11941
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(11951..12004)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    12028..12053
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    12060..12093
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    12095..12898
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 aagggcgaat tcaaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
       61 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      121 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      181 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      241 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      301 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
      361 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
      421 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
      481 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
      541 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
      601 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
      661 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
      721 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
      781 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
      841 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
      901 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
      961 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1021 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1081 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1141 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1201 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     1261 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1321 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1381 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1441 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1501 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1561 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1621 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1681 gcaacgcggc ctttttacgg ttcctggcga attcgccctt agagcagagc caggaacccc
     1741 tgtagggaag gggcaggaga gccaggggca tgagatggtg gacgaggaag ggggacaggg
     1801 aagcctgagc gcctctcctg ggcttgccaa ggactcaaac ccagaagccc agagcagggc
     1861 cttagggaag cgggaccctg ctctgggcgg aggaatatgt cccagatagc actggggact
     1921 ctttaaggaa agaaggatgg agaaagagaa agggagtaga ggcggccacg acctggtgaa
     1981 cacctaggac gcaccattct cacaaaggga gttttccaca cggacacccc cctcctcacc
     2041 acagccctgc caggacgggg ctggctactg gccttatctc acaggtaaaa ctgacgcacg
     2101 gaggaacaat ataaattggg gactagaaag gtgaagagcc aaagttagaa ctcaggacca
     2161 acttattctg attttgtttt tccaaactgc ttctcctctt gggaagtgta aggaagctgc
     2221 agcaccagga tcagtgaaac gcaccagaca gccgcgtcag agcagctcag gttctgggag
     2281 agggtagcgc agggtggcca ctgagaaccg ggcaggtcac gcatcccccc cttccctccc
     2341 accccctgcc aagctctccc tcccaggatc ctctctggct ccatcgtaag caaaccttag
     2401 aggttctggc aaggagagag atggctccag gaaatggggg tgtgtcacca gataaggaat
     2461 ctgcctaaca ggaggtgggg gttagaccca atatcaggag actaggaagg aggaggccta
     2521 aggatggggc ttttctgtca ccaatcctgt ccctagtaaa gctgatatct ccaccccaca
     2581 gtggtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     2641 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     2701 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     2761 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     2821 gagaacgtat aagctttagg cgtgtacggt gggcgcctat aaaagcagag ctcgtttagt
     2881 gaaccgtcag atcgcctgga gcaattccac aacacttttg tcttatacca actttccgta
     2941 ccacttccta ccctcgtaaa cttaaggccg ccaccatgta ttctccgctc tgtctcaccc
     3001 aggatgaatt tcatcctttc atcgaagcac ttctgcccca cgtccgagcc tttgcctaca
     3061 catggttcaa cctgcaggcc cgaaaacgaa aatacttcaa aaaacatgaa aagcgtatgt
     3121 caaaagaaga agagagagcc gtgaaggatg aattgctaag tgaaaaacca gaggtcaagc
     3181 agaagtgggc atctcgactt ctggcaaagt tgcggaaaga tatccgaccc gaatatcgag
     3241 aggattttgt tcttacagtt acagggaaaa aacctccatg ttgtgttctt tccaacccag
     3301 accagaaagg caagatgcga agaattgact gcctccgcca ggcagataaa gtctggaggt
     3361 tggaccttgt tatggtgatt ttgtttaaag gtattccgct ggaaagtact gatggcgagc
     3421 gccttgtaaa gtccccacaa tgctctaatc cagggctctg tgtccaaccc catcacatag
     3481 gggtttctgt taaggaactc gatttatatt tggcatactt tgtgcatgca gcagattcaa
     3541 gtcaatctga aagtcccagc cagccaagtg acgctgacat taaggaccag ccagaaaatg
     3601 gacatttggg cttccaggac agttttgtca catcaggtgt ttttagtgtc actgagctag
     3661 taagagtgtc acagacacca atagctgcag gaactggccc aaatttttct ctctcagatt
     3721 tggaaagttc ttcatactac agcatgagtc caggagcaat gaggaggtct ttacccagca
     3781 catcctctac gagctccaca aagcgcctca agtctgtgga ggatgaaatg gacagtcctg
     3841 gtgaggagcc attttataca ggccaagggc gctccccagg aagtggcagt cagtcaagtg
     3901 gatggcatga agtggagcca ggaatgccat ctccaaccac actgaagaag tcggagaagt
     3961 ctggtttcag cagcccctcc ccttcacaga cctcctccct gggaacggcg ttcacacagc
     4021 atcaccgacc tgtcattaca ggacccagag caagtccgca tgcaacacca tcgactcttc
     4081 atttcccgac atcacccatt atccagcagc ctgggcctta cttctcacac ccagccatcc
     4141 gctatcaccc tcaggagacg ctgaaagaat ttgtccaact tgtctgccct gatgctggtc
     4201 agcaggctgg acaggtgggg ttcctcaatc ccaatgggag cagccaaggc aaggtgcaca
     4261 acccattcct tcccacccca atgttgccac cgccaccgcc accaccgatg gccaggcctg
     4321 tgcctctgcc ggtgccagac acaaagcctc caaccacgtc aacagaagga ggtgcagcct
     4381 cccccacgtc accaacctac tcgacaccca gcacctcccc cgcaaaccga ttcgtcagtg
     4441 ttggaccacg ggatccaagc tttgtaaata tccctcaaca gacacagtcc tggtacctgg
     4501 gaggtagcgg agagggcaga ggcagtctgc tgacatgcgg tgacgtggaa gagaatcccg
     4561 gccctatgaa tctcctggac cccttcatga agatgaccga cgagcaggag aagggcctgt
     4621 ccggcgcccc cagccccacc atgtccgagg actccgcggg ctcgccctgc ccgtcgggct
     4681 ccggctcgga caccgagaac acgcggcccc aggagaacac gttccccaag ggcgagcccg
     4741 atctgaagaa ggagagcgag gaggacaagt tccccgtgtg catccgcgag gcggtcagcc
     4801 aggtgctcaa aggctacgac tggacgctgg tgcccatgcc ggtgcgcgtc aacggctcca
     4861 gcaagaacaa gccgcacgtc aagcggccca tgaacgcctt catggtgtgg gcgcaggcgg
     4921 cgcgcaggaa gctcgcggac cagtacccgc acttgcacaa cgccgagctc agcaagacgc
     4981 tgggcaagct ctggagactt ctgaacgaga gcgagaagcg gcccttcgtg gaggaggcgg
     5041 agcggctgcg cgtgcagcac aagaaggacc acccggatta caagtaccag ccgcggcgga
     5101 ggaagtcggt gaagaacggg caggcggagg cagaggaggc cacggagcag acgcacatct
     5161 cccccaacgc catcttcaag gcgctgcagg ccgactcgcc acactcctcc tccggcatga
     5221 gcgaggtgca ctcccccggc gagcactcgg ggcaatccca gggcccaccg accccaccca
     5281 ccacccccaa aaccgacgtg cagccgggca aggctgacct gaagcgagag gggcgcccct
     5341 tgccagaggg gggcagacag ccccctatcg acttccgcga cgtggacatc ggcgagctga
     5401 gcagcgacgt catctccaac atcgagacct tcgatgtcaa cgagtttgac cagtacctgc
     5461 cgcccaacgg ccacccgggg gtgccggcca cgcacggcca ggtcacctac acgggcagct
     5521 acggcatcag cagcaccgcg gccaccccgg cgagcgcggg ccacgtgtgg atgtccaagc
     5581 agcaggcgcc gccgccaccc ccgcagcagc ccccacaggc cccgccggcc ccgcaggcgc
     5641 ccccgcagcc gcaggcggcg cccccacagc agccggcggc acccccgcag cagccacagg
     5701 cgcacacgct gaccacgctg agcagcgagc cgggccagtc ccagcgaacg cacatcaaga
     5761 cggagcagct gagccccagc cactacagcg agcagcagca gcactcgccc caacagatcg
     5821 cctacagccc cttcaacctc ccacactaca gcccctccta cccgcccatc acccgctcac
     5881 agtacgacta caccgaccac cagaactcca gctcctacta cagccacgcg gcaggccagg
     5941 gcaccggcct ctactccacc ttcacctaca tgaaccccgc tcagcgcccc atgtacaccc
     6001 ccatcgccga cacctctggg gtcccttcca tcccgcagac ccacagcccc cagcactggg
     6061 aacaacccgt ctacacacag ctcactcgac cttgaatcga tcgcccaccc caacttgttt
     6121 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6181 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6241 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6301 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6361 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6421 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6481 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6541 gttcggctgc ggcgagcggt atcagctcac tcaaaggacg gccgcacatg taaggaaaat
     6601 tttagggatg ttaaagaaaa aaataacaca aaacaaaata taaaaaaaat ctaacctcaa
     6661 gtcaaggctt ttctatggaa taaggaatgg acagcagggg gctgtttcat atactgatga
     6721 cctctttata gccaaccttt gttcatggca gccagcatat gggcatatgt tgccaaactc
     6781 taaaccaaat actcattctg atgttttaaa tgatttgccc tcccatatgt ccttccgagt
     6841 gagagacaca aaaaattcca acacactatt gcaatgaaaa taaatttcct ttattagcca
     6901 gaagtcagat gctcaagggg cttcatgatg tccccataat ttttggcaga gggaaaaaga
     6961 tctcagtggt atttgtgagc cagggcattg gccacaccag ccaccacctt ctgataggca
     7021 gcctgcacct gaggagtgaa ttcacgcgtt tagcctggca gcatatcgag atcgaagtca
     7081 tcgagagcgt cagcagggag catatccaga tcaaaatcat ccagggcatc ggcgggcagc
     7141 atgtccaggt cgaagtcgtc cagggcgtcg gtgggtccgc cgctctcgca cttcagctgc
     7201 ttctcgaggc cgcagatgat cagttccagg ccgaacagga aggcgggctc ggcgccctgt
     7261 ctgtcgaaca gctcgatggc ctgcttcagc agggggggca tgctgtcggt ggtgggtgtc
     7321 tctctttcct ctttggcgac ctggtgttcc tgttcttcca gcacgcagcc cagggtgaag
     7381 tggcccacgg cgctcagggc gtacagggcg ttttccaggc tgaagccctg ctggcacagg
     7441 aaggccagct ggttttccag tgtctcgtac tgcttctcgg tgggtctggt gcccaggtgc
     7501 actttggcgc cgtcccggtg gctcagcagg gcgcatctgt agctcttggc gttgttccgc
     7561 aggaagtcct gccagctctc gccttccaga gggcagctgt gggtgtggtg ccggtccagc
     7621 atctcgatgg gcagggcgtc cagcagggcc cgcttgttct tcacgtgcca gtacagggtg
     7681 ggctgttcca cgcccagttt ctgggccagc ttccgggtgg tcaggccctc gatgcccacg
     7741 ccgttcagca gttccagggc gctgttgatc actttgctct tgtccagccg gctcatgtcg
     7801 acattctttg ccaaaatgat gagacagcac aataaccagc acgttgccca ggagctgtag
     7861 gaaaaagaag aaggcatgaa catggttagc agaggctcta gagccgccgg tcacacgcca
     7921 gaagccgaac cccgccctgc cccgtccccc ccgaaggcag ccgtcccccc gcggacagcc
     7981 ccgaggctgg agagggagaa ggggacggcg gcgcggcgac gcacgaaggc cctccccgcc
     8041 catttccttc ctgccggcgc cgcaccgctt cgccccgcgc ccgctagagg gggtgcggcg
     8101 gcgcctccca gatttcggct ccgcacagat ttgggacaaa ggaagtccct gcgccctctc
     8161 gcacgattac cataaaaggc aatggctgcg gctcgccgcg cctcgacagc cgccggcgct
     8221 ccgggggccg ccgcgcccct cccccgagcc ctccccggcc cgaggcggcc ccgccccgcc
     8281 cggcaccccc acctgccgcc accccccgcc cggcacggcg agccccgcgc cacgccccgt
     8341 acggagcccc gcacccgaag ccgggccgtg ctcagcaact cggggagggg ggtgcagggg
     8401 gggttgcagc ccgaccgacg cgcccacacc ccctgctcac ccccccacgc acacaccccg
     8461 cacgcagcct ttgttcccct cgcagccccc cccgcaccgc ggggcaccgc ccccggccgc
     8521 gctcccctcg cgcacactgc ggagcgcaca aagccccgcg ccgcgcccgc agcgctcaca
     8581 gccgccgggc agcgcggagc cgcacgcggc gctccccacg cacacacaca cgcacgcacc
     8641 ccccgagccg ctccccccgc acaaagggcc ctcccggagc ccctcaaggc tttcacgcag
     8701 ccacagaaaa gaaacaagcc gtcattaaac caagcgctaa ttacagcccg gaggagaagg
     8761 gccgtcccgc ccgctcacct gtgggagtaa cgcggtcagt cagagccggg gcgggcggcg
     8821 cgaggcggcg gcggagcggg gcacggggcg aaggcagcgt cgcagcgact ccccgcccgc
     8881 cgcgcgcttc gctttttata gggccgccgc cgccgccgcc tcgccataaa aggaaacttt
     8941 cggagcgcgc cgctctgatt ggctgccgcc gcacctctcc gcctcgcccc gccccgcccc
     9001 tcgccccgcc ccgccccgcc tggcgcgcgc cccccccccc cccccgcccc catcgctgca
     9061 caaaataatt aaaaaataaa taaatacaaa attgggggtg gggagggggg ggagatgggg
     9121 agagtgaagc agaacgtggg gctcacctcg accatggtaa tagcgatgac taatacgtag
     9181 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
     9241 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
     9301 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
     9361 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
     9421 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
     9481 actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg tcgagaccca
     9541 tagagcccac cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt
     9601 cctgccccac cccacccccc agaatagaat gacacctact cagacaatgc gatgcaattt
     9661 cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag gaaggcacgg
     9721 gggaggggca aacaacagat ggctggcaac tagaaggcac agtcgaggct gatcagcggg
     9781 tttataactt cgtataatgt atgctatacg aagttatggt accttactcg tccatgccgc
     9841 cggtggagtg gcggccctcg gcgcgttcgt actgttccac gatggtgtag tcctcgttgt
     9901 gggaggtgat gtccaacttg atgttgacgt tgtaggcgcc gggcagctgc acgggcttct
     9961 tggccttgta ggtggtcttg acctcagcgt cgtagtggcc gccgtccttc agcttcagcc
    10021 tctgcttgat ctcgcccttc agggcgccgt cctcggggta catccgctcg gaggaggcct
    10081 cccagcccat ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg gtgccgcgca
    10141 gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc acggtcacca
    10201 cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct
    10261 tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg tacatgaact
    10321 gaggggacag gatgtcccag gcgaagggca gggggccacc cttggtcacc ttcagcttgg
    10381 cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg aactcgtggc
    10441 cgttcacgga gccctccatg tgcaccttga agcgcatgaa ctccttgatg atggccatgt
    10501 tatcctcctc gcccttgctc accatggtgg cgaccggtag cgctagcgta ggcgccggtc
    10561 acagcttgga tctgtaacgg cgcagaacag aaaacgaaac aaagacgtag agttgagcaa
    10621 gcagggtcag gcaaagcgtg gagagccggc tgagtctagg taggctccaa gggagcgccg
    10681 gacaaaggcc cggtctcgac ctgagcttta aacttaccta gacggcggac gcagttcagg
    10741 aggcaccaca ggcgggaggc ggcagaacgc gactcaaccg gcgtggatgg cggcctcagg
    10801 tagggcggcg ggcgcgtgaa ggagagatgc gagcccctcg aagcttcagc tgtgttctgg
    10861 cggcaaaccc gttgcgaaaa agaacgttca cggcgactac tgcacttata tacggttctc
    10921 ccccaccctc gggaaaaagg cggagccagt acacgacatc actttcccag tttaccccgc
    10981 gccaccttct ctaggcaccg gttcaattgc cgacccctcc ccccaacttc tcggggactg
    11041 tgggcgatgt gcgctctgcc cactgacggg caccggagcg atcgcagatc atgcatccat
    11101 agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc
    11161 ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc
    11221 ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg
    11281 ggaggggcaa acaacagatg gctggcaact agaaggcaca ggtttaaacg ggccctctag
    11341 atcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc acctcgacgt
    11401 cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc gcggaggtct
    11461 ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc acgacggcgc
    11521 tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg aaccacgcgg
    11581 gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg gccagccggg
    11641 aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc gcttcgacgc
    11701 tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac accttgccga
    11761 tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc tcgatgtggc
    11821 ggtccggatc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg gcgagggtgc
    11881 gtacggccct ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg tactcggtca
    11941 tctcgagcct agggccggga ttctcctcca cgtcaccgca tgttagaaga cttcctctgc
    12001 cctctccgct gccagatctc tcgaggccct gtgggaggaa gagaagaggt cagaagctta
    12061 taacttcgta taatgtatgc tatacgaagt tattgcccca ctgtggggtg gaggggacag
    12121 ataaaagtac ccagaaccag agccacatta accggccctg ggaatataag gtggtcccag
    12181 ctcggggaca caggatccct ggaggcagca aacatgctgt cctgaagtgg acataggggc
    12241 ccgggttgga ggaagaagac tagctgagct ctcggacccc tggaagatgc catgacaggg
    12301 ggctggaaga gctagcacag actagagagg taaggggggt aggggagctg cccaaatgaa
    12361 aggagtgaga ggtgacccga atccacagga gaacggggtg tccaggcaaa gaaagcaaga
    12421 ggatggagag gtggctaaag ccagggagac ggggtacttt ggggttgtcc agaaaaacgg
    12481 tgatgatgca ggcctacaag aaggggaggc gggacgcaag ggagacatcc gtcggagaag
    12541 gccatcctaa gaaacgagag atggcacagg ccccagaagg agaaggaaaa gggaacccag
    12601 cgagtgaaga cggcatgggg ttgggtgagg gaggagagat gcccggagag gacccagaca
    12661 cggggaggat ccgctcagag gacatcacgt ggtgcagcgc cgagaaggaa gtgctccgga
    12721 aagagcatcc ttgggcagca acacagcaga gagcaagggg aagagggagt ggaggaagac
    12781 ggaacctgaa ggaggcggca gggaaggatc tgggccagcc gtagaggtga cccaggccac
    12841 aagctgcaga cagaaagcgg cacaggccca ggggagagaa tgctggtcag agaaagca
//
