LOCUS       sequence-360426-       17993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for expression of PULSE system (under the control of 
            CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc
            and a plant selection cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17993)
  AUTHORS   Ochoa-Fernandez R, Abel NB, Wieland FG, Schlegel J, Koch LA, Miller 
            JB, Engesser R, Giuriani G, Brandl SM, Timmer J, Weber W, Ott T, 
            Simon R, Zurbriggen MD
  TITLE     Optogenetic control of gene expression in plants in the presence of 
            ambient white light.
  JOURNAL   Nat Methods. 2020 Jul;17(7):717-725. doi: 10.1038/s41592-020-0868-y.
            Epub 2020 Jun 29.
  PUBMED    32601426
REFERENCE   2  (bases 1 to 17993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0868-y"; journalName: "Nat Methods"; date: 
            "2020-07"; volume: "17"; issue: "7"; pages: "717-725"
COMMENT     Sequence Label: BM00654
FEATURES             Location/Qualifiers
     source          1..17993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     477..501
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(640..658)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2573..2747
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        2848..3193
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3234..3254
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      3909..4161
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        4265..4610
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             6763..6996
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             6997..7017
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7033..7285
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             8833..9768
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     terminator      9789..10041
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        10145..10490
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             11434..11454
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      11470..11722
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        11825..12004
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             12026..12820
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12080..12099)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12690..12709
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      12833..13085
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    13180..13204
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             13468..14097
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             14526..15599
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      15665..15859
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        16142..16246
                     /gene="bla"
                     /label=AmpR promoter
     CDS             16247..17041
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      17212..17800
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17701..17720
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    17923..17947
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagcttcga agtttaaacg attgaatata accgacgtga ctgttacatt tagggattga
       61 atataaccga cgtgactgtt acatttaggg attgaatata accgacgtga ctgttacatt
      121 tagggattga atataaccga cgtgactgtt acatttaggg ataaacgatt gaatataacc
      181 gacgtgactg ttacatttag ggattgaata taaccgacgt gactgttaca tttagggatt
      241 gaatataacc gacgtgactg ttacatttag ggattgaata taaccgacgt gactgttaca
      301 tttagggatg ctagcctcga gtaggtagcc tttagtccat gcgttatagg tagcctttag
      361 tccatgcgtt ataggtagcc tttagtccat gcgttatagg tagcctttag tccatgcgtt
      421 ataggtagcc tttagtccat gaagcttaga cactagagct agccctatat aagcagagct
      481 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      541 agacaccggg accgatccag cctaatgact agtatggaag atgccaaaaa cataaagaaa
      601 ggcccggcgc cattctatcc gctggaagat ggaaccgctg gagagcaact gcataaggct
      661 atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg
      721 gacatcactt acgctgaggt aagtttctgt gttccttatt ctctcaaaat cttcgatttt
      781 gttttcgttc gatcccaatt tcgtatatgt tctttggttt agattctgtt aatcttagat
      841 cgaagaggat tttctgggtt tgatcgttag atatcatctt aattctcgat tagggtttca
      901 tagatatcat ccgatttgtt caaataattt gagttttgtc gaataattac tcttcgactt
      961 gtgatttcta tctagatctg gtgttagttt ctagtttgtg cgatcgaatt tgtcgattaa
     1021 tccgagtttt tctgattaac agtacttcga aatgtccgtt cggttggcag aagctatgaa
     1081 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt
     1141 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat
     1201 ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt
     1261 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa
     1321 aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt
     1381 cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag
     1441 ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt
     1501 cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg
     1561 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg
     1621 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt
     1681 tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt
     1741 gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa
     1801 tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc
     1861 caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc
     1921 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt
     1981 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg
     2041 cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac
     2101 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga
     2161 ggacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca
     2221 ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg
     2281 tgtcgcaggt ctacccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga
     2341 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac
     2401 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg
     2461 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat
     2521 cgccgtgtaa cctgcagggc ttctctagct agagtcgatc gacaagctcg agtttctcca
     2581 taataatgtg tgagtagttc ccagataagg gaattagggt tcctataggg tttcgctcat
     2641 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     2701 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatcgct ctcgaggaat
     2761 tccacaggag gtcaacatgg tggagcacga cactctggtc tactccaaaa atgtcaaaga
     2821 tacagtctca gaagatcaaa gggctattga gacttttcaa caaaggataa tttcgggaaa
     2881 cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga
     2941 aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatctctc
     3001 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
     3061 ggttccaacc acgtctacaa agcaagtgga ttgatgtgac atctccactg acgtaaggga
     3121 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     3181 tttggagagg acacgcaatg atgcttgatc ttgaacttag acttggtttt gctccaaaga
     3241 agaagaggaa ggtgggagct ggagctgggg cagacgacac acgcgttgag gtgcaaccgc
     3301 cggcgcagtg ggtcctcgac ctgatcgagg ccagcccgat cgcatcggtc gtgtccgatc
     3361 cgcgtctcgc cgacaatccg ctgatcgcca tcaaccaggc cttcaccgac ctgaccggct
     3421 attccgaaga agaatgcgtc ggccgcaatt gccgattcct ggcaggttcc ggcaccgagc
     3481 cgtggctgac cgacaagatc cgccaaggcg tgcgcgagca caagccggtg ctggtcgaga
     3541 tcctgaacta caagaaggac ggcacgccgt tccgcaatgc cgtgctcgtt gcaccgatct
     3601 acgatgacga cgacgagctt ctctatttcc tcggcagcca ggtcgaagtc gacgacgacc
     3661 agcccaacat gggcatggcg cgccgcgaac gcgccgcgga aatgctcaag acgctgtcgc
     3721 cgcgccagct cgaggttacg acgctggtgg catcgggctt gcgcaacaag gaagtggcgg
     3781 cccggctcgg cctgtcggag aaaaccgtca agatgcaccg cgggctggtg atggaaaagc
     3841 tcaacctgaa gaccagtgcc gatctggtgc gcattgccgt cgaagccgga atctaagctt
     3901 gtcaagcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     3961 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     4021 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     4081 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     4141 tgtcatctat gttactagat cgacgctgca tgcgaattct ctgggaggtc aacatggtgg
     4201 agcacgacac tctggtctac tccaaaaatg tcaaagatac agtctcagaa gatcaaaggg
     4261 ctattgagac ttttcaacaa aggataattt cgggaaacct cctcggattc cattgcccag
     4321 ctatctgtca cttcatcgaa aggacagtag aaaaggaagg tggctcctac aaatgccatc
     4381 attgcgataa aggaaaggct atcattcaag atctctctgc cgacagtggt cccaaagatg
     4441 gacccccacc cacgaggagc atcgtggaaa aagaagaggt tccaaccacg tctacaaagc
     4501 aagtggattg atgtgacatc tccactgacg taagggatga cgcacaatcc cactatcctt
     4561 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca cgcaatgaca
     4621 acaatggttt ccggagtcgg gggtagtggc ggtggccgtg gcggtggccg tggcggagaa
     4681 gaagaaccgt cgtcaagtca cactcctaat aaccgaagag gaggagaaca agctcaatcg
     4741 tcgggaacga aatctctcag accaagaagc aacactgaat caatgagcaa agcaattcaa
     4801 cagtacaccg tcgacgcaag actccacgcc gttttcgaac aatccggcga atcagggaaa
     4861 tcattcgact actcacaatc actcaaaacg acgacgtacg gttcctctgt acctgagcaa
     4921 cagatcacag cttatctctc tcgaatccag cgaggtggtt acattcagcc tttcggatgt
     4981 atgatcgccg tcgatgaatc cagtttccgg atcatcggtt acagtgaaaa cgccagagaa
     5041 atgttaggga ttatgcctca atctgttcct actcttgaga aacctgagat tctagctatg
     5101 ggaactgatg tgagatcttt gttcacttct tcgagctcga ttctactcga gcgtgctttc
     5161 gttgctcgag agattacctt gttaaatccg gtttggatcc attccaagaa tactggtaaa
     5221 ccgttttacg ccattcttca taggattgat gttggtgttg ttattgattt agagccagct
     5281 agaactgaag atcctgcgct ttctattgct ggtgctgttc aatcgcagaa actcgcggtt
     5341 cgtgcgattt ctcagttaca ggctcttcct ggtggagata ttaagctttt gtgtgacact
     5401 gtcgtggaaa gtgtgaggga cttgactggt tatgatcgtg ttatggttta taagtttcat
     5461 gaagatgagc atggagaagt tgtagctgag agtaaacgag atgatttaga gccttatatt
     5521 ggactgcatt atcctgctac tgatattcct caagcgtcaa ggttcttgtt taagcagaac
     5581 cgtgtccgaa tgatagtaga ttgcaatgcc acacctgttc ttgtggtcca ggacgatagg
     5641 ctaactcagt ctatgtgctt ggttggttct actcttaggg ctcctcatgg ttgtcactct
     5701 cagtatatgg ctaacatggg atctattgcg tctttagcaa tggcggttat aatcaatgga
     5761 aatgaagatg atgggagcaa tgtagctagt ggaagaagct cgatgaggct ttggggtttg
     5821 gttgtttgcc atcacacttc ttctcgctgc ataccgtttc cgctaaggta tgcttgtgag
     5881 tttttgatgc aggctttcgg tttacagtta aacatggaat tgcagttagc tttgcaaatg
     5941 tcagagaaac gcgttttgag aacgcagaca ctgttatgtg atatgcttct gcgtgactcg
     6001 cctgctggaa ttgttacaca gagtcccagt atcatggact tagtgaaatg tgacggtgca
     6061 gcatttcttt accacgggaa gtattacccg ttgggtgttg ctcctagtga agttcagata
     6121 aaagatgttg tggagtggtt gcttgcgaat catgcggatt caaccggatt aagcactgat
     6181 agtttaggcg atgcggggta tcccggtgca gctgcgttag gggatgctgt gtgcggtatg
     6241 gcagttgcat atatcacaaa aagagacttt cttttttggt ttcgatctca cactgcgaaa
     6301 gaaatcaaat ggggaggcgc taagcatcat ccggaggata aagatgatgg gcaacgaatg
     6361 catcctcgtt cgtcctttca ggcttttctt gaagttgtta agagccggag tcagccatgg
     6421 gaaactgcgg aaatggatgc gattcactcg ctccagctta ttctgagaga ctcttttaaa
     6481 gaatctgagg cggctatgaa ctctaaagtt gtggatggtg tggttcagcc atgtagggat
     6541 atggcggggg aacaggggat tgatgagtta ggtgaattcg atagtgctgg tagtgctggt
     6601 agtgctggtt ccgcgtacag ccgcgcgcgt acgaaaaaca attacgggtc taccatcgag
     6661 ggcctgctcg atctcccgga cgacgacgcc cccgaagagg cggggctggc ggctccgcgc
     6721 ctgtcctttc tccccgcggg acacacgcgc agactgtcga cggccccccc gaccgatgtc
     6781 agcctggggg acgagctcca cttagacggc gaggacgtgg cgatggcgca tgccgacgcg
     6841 ctagacgatt tcgatctgga catgttgggg gacggggatt ccccgggtcc gggatttacc
     6901 ccccacgact ccgcccccta cggcgctctg gatatggccg acttcgagtt tgagcagatg
     6961 tttaccgatg cccttggaat tgacgagtac ggtgggccaa agaagaagag gaaggtgtag
     7021 gcttgtcaag cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     7081 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     7141 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     7201 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     7261 gcggtgtcat ctatgttact agatcgacgc tccatgggaa ttcgtaagga ggacgtcgtt
     7321 gtggttggtg ctttccttac attctgagcc tctttccttc taatccactc atctgcatct
     7381 tcttgtgtcc ttactaatac ctcattggtt ccaaattccc tccctttaag caccagctcg
     7441 tttctgttct tccacagcct cccaagtatc caagggacta aagcctccac attcttcaga
     7501 tcaggatatt cttgtttaag atgttgaact ctatggaggt ttgtatgaac tgatgatcta
     7561 ggaccggata agttcccttc ttcatagcga acttattcaa agaatgtttt gtgtatcatt
     7621 cttgttacat tgttattaat gaaaaaatat tattggtcat tggactgaac acgagtgtta
     7681 aatatggacc aggccccaaa taagatccat tgatatatga attaaataac aagaataaat
     7741 cgagtcacca aaccacttgc cttttttaac gagacttgtt caccaacttg atacaaaagt
     7801 cattatccta tgcaaatcaa taatcataca aaaatatcca ataacactaa aaaaattaaa
     7861 agaaatggat aatttcacaa tatgttatac gataaagaag ttacttttcc aagaaattca
     7921 ctgattttat aagcccactt gcattagata aatggcaaaa aaaaacaaaa aggaaaagaa
     7981 ataaagcacg aagaattcta gaaaatacga aatacgcttc aatgcagtgg gacccacggt
     8041 tcaattattg ccaattttca gctccaccgt atatttaaaa aataaaacga taatgctaaa
     8101 aaaatataaa tcgtaacgat cgttaaatct caacggctgg atcttatgac gaccgttaag
     8161 gaaattgtgg ttgtcggacg aagtccagta ataaacggcg tcaaagtggt tgcagccggc
     8221 acacacgagt cgtgtttatc aactcaaagc acaaatactt ttcctcaacc taaaaataag
     8281 gcaattagcc aaaaacaact ttgcgtgtaa acaacgctca atacacgtgt cattttatta
     8341 ttagctattg cttcaccgcc ttagctttct cgtgacctag tcgtcctcgt cttttcttct
     8401 tcttcttcta taaaacaata cccaaagagc tcttcttctt cacaattcag atttcaattt
     8461 ctcaaaatct taaaaacttt ctctcaattc tctctaccgt gatcaaggta aatttctgtg
     8521 ttccttattc tctcaaaatc ttcgattttg ttttcgttcg atcccaattt cgtatatgtt
     8581 ctttggttta gattctgtta atcttagatc gtagacgatt ttctgggttt gatcgttaga
     8641 tatcatctta attctcgatt agggtttcat agatatcatc cgatttgttc aaataatttg
     8701 agttttgtcg aataattact cttcgatttg tgatttctat ctagatctgg tgttagtttc
     8761 tagtttgtgc gatcgaattt gtcgattaat ctgagttttt ctgattaaca gatgcagatc
     8821 ttaatgtcgc gaatggcttc gaaagtttat gatccagaac aaaggaaacg gatgataact
     8881 ggtccgcagt ggtgggccag atgtaaacaa atgaatgttc ttgattcatt tattaattat
     8941 tatgattcag aaaaacatgc agaaaatgct gttatttttt tacatggtaa cgcggcctct
     9001 tcttatttat ggcgacatgt tgtgccacat attgagccag tagcgcggtg tattatacca
     9061 gaccttattg gtatgggcaa atcaggcaaa tctggtaatg gttcttatag gttacttgat
     9121 cattacaaat atcttactgc atggtttgaa cttcttaatt taccaaagaa gatcattttt
     9181 gtcggccatg attggggtgc ttgtttggca tttcattata gctatgagca tcaagataag
     9241 atcaaagcaa tagttcacgc tgaaagtgta gtagatgtga ttgaatcatg ggatgaatgg
     9301 cctgatattg aagaagatat tgcgttgatc aaatctgaag aaggagaaaa aatggttttg
     9361 gagaataact tcttcgtgga aaccatgttg ccatcaaaaa tcatgagaaa gttagaacca
     9421 gaagaatttg cagcatatct tgaaccattc aaagagaaag gtgaagttcg tcgtccaaca
     9481 ttatcatggc ctcgtgaaat cccgttagta aaaggtggta aacctgacgt tgtacaaatt
     9541 gttaggaatt ataatgctta tctacgtgca agtgatgatt taccaaaaat gtttattgaa
     9601 tcggacccag gattcttttc caatgctatt gttgaaggtg ccaagaagtt tcctaatact
     9661 gaatttgtca aagtaaaagg acttcatttt tcgcaagaag atgcacctga tgaaatggga
     9721 aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg aacaataggc gatcgcgctt
     9781 gtcaagcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     9841 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     9901 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     9961 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
    10021 tgtcatctat gttactagat cgacgctgag ctcgaattct agtggaggtc aacatggtgg
    10081 agcacgacac tctggtctac tccaaaaatg tcaaagatac agtctcagaa gatcaaaggg
    10141 ctattgagac ttttcaacaa aggataattt cgggaaacct cctcggattc cattgcccag
    10201 ctatctgtca cttcatcgaa aggacagtag aaaaggaagg tggctcctac aaatgccatc
    10261 attgcgataa aggaaaggct atcattcaag atctctctgc cgacagtggt cccaaagatg
    10321 gacccccacc cacgaggagc atcgtggaaa aagaagaggt tccaaccacg tctacaaagc
    10381 aagtggattg atgtgacatc tccactgacg taagggatga cgcacaatcc cactatcctt
    10441 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca cgcaatgaca
    10501 acaatgcccc gccccaagct caagtccgat gacgaggtac tcgaggccgc caccgtagtg
    10561 ctgaagcgtt gcggtcccat agagttcacg ctcagcggag tagcaaagga ggtggggctc
    10621 tcccgcgcag cgttaatcca gcgcttcacc aaccgcgata cgctgctggt gaggatgatg
    10681 gagcgcggcg tcgagcaggt gcggcattac ctgaatgcga taccgatagg cgcagggccg
    10741 caagggctct gggaattttt gcaggtgctc gttcggagca tgaacactcg caacgacttc
    10801 tcggtgaact atctcatctc ctggtacgag ctccaggtgc cggagctacg cacgcttgcg
    10861 atccagcgga accgcgcggt ggtggagggg atccgcaagc gactgccccc aggtgctcct
    10921 gcggcagctg agttgctcct gcactcggtc atcgctggcg cgacgatgca gtgggccgtc
    10981 gatccggatg gtgagctagc tgatcatgtg ctggctcaga tcgctgccat cctgtgttta
    11041 atgtttcccg aacacgacga tttccaactc ctccaggcac atgcgtccgc gtacagctta
    11101 attaacagtg ctggtagtgc tggtagtgct ggtatgatgt tcttaccaac cgattattgt
    11161 tgcaggttaa gcgatcaaga gtatatggag cttgtgtttg agaatggcca gattcttgca
    11221 aagggccaaa gatccaacgt ttctctgcat aatcaacgta ccaaatcgat catggatttg
    11281 tatgaggcag agtataacga ggatttcatg aagagtatca tccatggtgg tggtggtgcc
    11341 atcacaaatc tcggggacac gcaggttgtt ccacaaagtc atgttgctgc tgcccatgaa
    11401 acaaacatgt tggaaagcaa taaacatgtt gacccaaaga agaagaggaa ggtgtaggct
    11461 tgtcaagcag atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
    11521 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
    11581 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
    11641 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
    11701 gtgtcatcta tgttactaga tcgacgctaa gcttgaattc ttgcggagag cggagaatta
    11761 agggagtcac gttatgaccc ccgccgatga cgcgggacaa gccgttttac gtttggaact
    11821 gacagaaccg caacgttgaa ggagccactc agccgcgggt ttctggagtt taatgagcta
    11881 agcacatacg tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc actatcagct
    11941 agcaaatatt tcttgtcaaa aatgctccac tgacgttcca taaattcccc tcggtatcca
    12001 attagagtct catattcact ctcaaatgat tgaacaagat ggattgcacg caggttctcc
    12061 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
    12121 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
    12181 cctgtccggt gccctgaatg aactccaaga cgaggcagcg cggctatcgt ggctggccac
    12241 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
    12301 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
    12361 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
    12421 attcgaccac caagcgaaac atcgcatcga gcgaggacgt actcggatgg aagccggtct
    12481 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
    12541 caggctcaag gcgcggatgc ccgacggcga ggatctcgtc gtgacccagg gcgatgcctg
    12601 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
    12661 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
    12721 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
    12781 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcttgtcaag cagatcgttc
    12841 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
    12901 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
    12961 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
    13021 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
    13081 agatcgacgc tggatccgaa ttcggcaggg cccactgcat ccaccccagt acattaaaaa
    13141 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
    13201 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
    13261 aggcagccca tcagccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct
    13321 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg
    13381 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt
    13441 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc
    13501 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc
    13561 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg
    13621 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag
    13681 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct
    13741 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac
    13801 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga
    13861 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag
    13921 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc
    13981 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc
    14041 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt
    14101 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg
    14161 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg
    14221 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag
    14281 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca
    14341 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa
    14401 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
    14461 gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
    14521 tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
    14581 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
    14641 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
    14701 cgccggcagc cggtgcgtcg tcgattagga agccgcccaa gggcgacgag caaccagatt
    14761 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
    14821 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
    14881 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
    14941 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
    15001 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
    15061 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
    15121 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
    15181 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
    15241 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
    15301 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
    15361 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
    15421 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
    15481 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
    15541 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
    15601 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaagcttct
    15661 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
    15721 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
    15781 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
    15841 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
    15901 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
    15961 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
    16021 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cgagctccca tccccccagg
    16081 ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat ggcagcgctg gccaattcgt
    16141 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
    16201 acaataacct tgataaatgc ttcaataata ttgaaaaagg aagagtatgg ctaaaatgag
    16261 aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgcaaaag atacggaagg
    16321 aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat
    16381 gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct
    16441 atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg
    16501 gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga
    16561 acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat
    16621 cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta
    16681 cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagagg acactccatt
    16741 taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt
    16801 cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg
    16861 ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt
    16921 ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact
    16981 ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta
    17041 gctgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    17101 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    17161 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    17221 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    17281 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    17341 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    17401 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    17461 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    17521 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    17581 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    17641 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    17701 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    17761 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    17821 tttacggttc ctgtggttgg cacatacaaa tggacgaacg gataaacctt ttcacgccct
    17881 tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat
    17941 cctgtcaaac actgatagtt taaactccct cggtcacatg tgcatcctcg ctc
//
