LOCUS       sequence-360438-        9989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CD74-ROS1 fusion (C6;R34) harboring ROS1 G2032R mutation in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Lin JJ, Choudhury NJ, Yoda S, Zhu VW, Johnson TW, Sakhtemani R, 
            Dagogo-Jack I, Digumarthy SR, Lee C, Do A, Peterson J, 
            Prutisto-Chang K, Malik W, Hubbeling HG, Langenbucher A, Schoenfeld 
            AJ, Falcon CJ, Temel JS, Sequist LV, Yeap BY, Lennerz JK, Shaw AT, 
            Lawrence MS, Ou SI, Hata AN, Drilon A, Gainor JF
  TITLE     Spectrum of Mechanisms of Resistance to Crizotinib and Lorlatinib in
            ROS1 Fusion-Positive Lung Cancer.
  JOURNAL   Clin Cancer Res. 2021 May 15;27(10):2899-2909. doi: 
            10.1158/1078-0432.CCR-21-0032. Epub 2021 Mar 8.
  PUBMED    33685866
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/1078-0432.CCR-21-0032"; journalName: "Clin Cancer Res"; 
            date: "2021-05-15- 15"; volume: "27"; issue: "10"; pages: 
            "2899-2909"
COMMENT     Sequence Label: pLenti-CD74-ROS1 G2032R
FEATURES             Location/Qualifiers
     source          1..9989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     300..319
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        300..318
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    348..372
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2521..2545)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2577..2600
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2616..2642
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2616..2642
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2649..3215
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2665..3217
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2809..2826)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3036..3055
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3229..3828
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3229..3248)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3725..3745
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3845..4433
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3898..3918)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4316..4327)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4508..4741
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4860..4878)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4946..5050
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5051..5119,5120..5911)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5269..5288)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6082..6670
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6571..6590
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6824..6841
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7081..7102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7117..7122,7123..7140,7141..7147)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7155..7171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7160..7182
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7179..7195
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7179..7195
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7245..7264
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7456..8089
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8136..8261
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8758..8991
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9176..9220
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9517..9634
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        9686..9989
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cggcggccgc agatctctcg aggttgatct
      301 aatacgactc actataggga gacccaagct ggctagttaa gctatcaaca agtttgtaca
      361 aaaaagcagg ctccgcggcc gcccccttca ccatgcacag gaggagaagc aggagctgtc
      421 gggaagatca gaagccagtc atggatgacc agcgcgacct tatctccaac aatgagcaac
      481 tgcccatgct gggccggcgc cctggggccc cggagagcaa gtgcagccgc ggagccctgt
      541 acacaggctt ttccatcctg gtgactctgc tcctcgctgg ccaggccacc accgcctact
      601 tcctgtacca gcagcagggc cggctggaca aactgacagt cacctcccag aacctgcagc
      661 tggagaacct gcgcatgaag cttcccaagc ctcccaagcc tgtgagcaag atgcgcatgg
      721 ccaccccgct gctgatgcag gcgctgccca tgggagccct gccccagggg cccatgcaga
      781 atgccaccaa gtatggcaac atgacagagg accatgtgat gcacctgctc cagaatgctg
      841 accccctgaa ggtgtacccg ccactgaagg ggagcttccc ggagaacctg agacacctta
      901 agaacaccat ggagaccata gactggaagg tctttgagag ctggatgcac cattggctcc
      961 tgtttgaaat gagcaggcac tccttggagc aaaagcccac tgacgctcca ccgaaagatg
     1021 atttttggat accagaaaca agtttcatac ttactattat agttggaata tttctggttg
     1081 ttacaatccc actgaccttt gtctggcata gaagattaaa gaatcaaaaa agtgccaagg
     1141 aaggggtgac agtgcttata aacgaagaca aagagttggc tgagctgcga ggtctggcag
     1201 ccggagtagg cctggctaat gcctgctatg caatacatac tcttccaacc caagaggaga
     1261 ttgaaaatct tcctgccttc cctcgggaaa aactgactct gcgtctcttg ctgggaagtg
     1321 gagcctttgg agaagtgtat gaaggaacag cagtggacat cttaggagtt ggaagtggag
     1381 aaatcaaagt agcagtgaag actttgaaga agggttccac agaccaggag aagattgaat
     1441 tcctgaagga ggcacatctg atgagcaaat ttaatcatcc caacattctg aagcagcttg
     1501 gagtttgtct gctgaatgaa ccccaataca ttatcctgga actgatggag ggaagagacc
     1561 ttcttactta tttgcgtaaa gcccggatgg caacgtttta tggtccttta ctcaccttgg
     1621 ttgaccttgt agacctgtgt gtagatattt caaaaggctg tgtctacttg gaacggatgc
     1681 atttcattca cagggatctg gcagctagaa attgccttgt ttccgtgaaa gactatacca
     1741 gtccacggat agtgaagatt ggagactttg gactcgccag agacatctat aaaaatgatt
     1801 actatagaaa gagaggggaa ggcctgctcc cagttcggtg gatggctcca gaaagtttga
     1861 tggatggaat cttcactact caatctgatg tatggtcttt tggaattctg atttgggaga
     1921 ttttaactct tggtcatcag ccttatccag ctcattccaa ccttgatgtg ttaaactatg
     1981 tgcaaacagg agggagactg gagccaccaa gaaattgtcc tgatgatctg tggaatttaa
     2041 tgacccagtg ctgggctcaa gaacccgacc aaagacctac ttttcataga attcaggacc
     2101 aacttcagtt attcagaaat tttttcttaa atagcattta taagtccaga gatgaagcaa
     2161 acaacagtgg agtcataaat gaaagctttg aaggtgaaga tggcgatgtg atttgtttga
     2221 attcagatga cattatgcca gttgctttaa tggaaacgaa gaaccgagaa gggttaaact
     2281 atatggtact tgctacagaa tgtggccaag gtgaagaaaa gtctgagggt cctctaggct
     2341 cccaggaatc tgaatcttgt ggtctgagga aagaagagaa ggaaccacat gcagacaaag
     2401 atttctgcca agaaaaacaa gtggcttact gcccttctgg caagcctgaa ggcctgaact
     2461 atgcctgtct cactcacagt ggatatggag atgggtctga ttaaaagggt gggcgcgccg
     2521 acccagcttt cttgtacaaa gtggtttagc tagagggccc gcggttcgaa ctcgaggact
     2581 acaaggacga cgatgacaag tcggccgctg gaggataccc ctacgacgtg cccgactacg
     2641 cctagaattc cgcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     2701 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     2761 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     2821 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     2881 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     2941 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     3001 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     3061 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     3121 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     3181 gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat gaccgagtac
     3241 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     3301 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     3361 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     3421 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     3481 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     3541 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     3601 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     3661 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     3721 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     3781 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatg catcgatgga
     3841 tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     3901 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3961 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4021 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4081 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4141 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4201 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     4261 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     4321 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     4381 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgagat
     4441 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     4501 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     4561 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     4621 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     4681 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     4741 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     4801 gagagtgaga ggcccgggtt aattaaggaa agggctagat cattcttgaa gacgaaaggg
     4861 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     4921 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4981 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5041 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5101 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5161 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5221 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5281 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5341 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5401 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5461 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5521 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5581 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5641 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5701 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5761 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5821 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5881 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5941 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6001 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6061 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6121 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6181 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     6241 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6301 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6361 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6421 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6481 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6541 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6601 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6661 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6721 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     6781 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     6841 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     6901 atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     6961 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     7021 aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     7081 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     7141 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
     7201 tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7261 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
     7321 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
     7381 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
     7441 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
     7501 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
     7561 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     7621 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     7681 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     7741 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     7801 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     7861 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     7921 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7981 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8041 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     8101 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     8161 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     8221 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     8281 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     8341 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     8401 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     8461 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     8521 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     8581 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     8641 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     8701 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     8761 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     8821 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     8881 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     8941 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     9001 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     9061 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     9121 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     9181 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     9241 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     9301 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     9361 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     9421 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     9481 ccgaattaat tcacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     9541 ggggtacagt gcagggaaaa gaatagtaga cataatagca acagacatac aaactaaaga
     9601 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     9661 tccagtttgg actagtggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     9721 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     9781 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     9841 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     9901 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     9961 tctacgtatt agtcatcgct attaccatg
//
