LOCUS       sequence-360441-       15785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. 
            cerevisiae; contains an oriT for transfer via conjugation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15785)
  AUTHORS   Brumwell SL, Van Belois KD, Giguere DJ, Edgell DR, Karas BJ
  TITLE     Conjugation-Based Genome Engineering in Deinococcus radiodurans.
  JOURNAL   ACS Synth Biol. 2022 Mar 18;11(3):1068-1076. doi: 
            10.1021/acssynbio.1c00524. Epub 2022 Mar 7.
  PUBMED    35254818
REFERENCE   2  (bases 1 to 15785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00524"; journalName: "ACS Synth Biol"; date: 
            "2022-03-18- 18"; volume: "11"; issue: "3"; pages: "1068-1076"
COMMENT     Sequence Label: pDEINO5
FEATURES             Location/Qualifiers
     source          1..15785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      14..628
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      105..636
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      704..923
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             1014..1769
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    1772..2022
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     misc_feature    2184..2687
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        2688..2874
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             2875..3537
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     CDS             3727..4902
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             4902..5873
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    5946..6419
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    6679..7077
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(7095..7128)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     7196..7215
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(7295..7570)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGETIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
                     AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS"
     primer_bind     7408..7430
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7422..7439
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7423..7439
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7446..7465
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7446..7464
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7467..7523
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7566..7582)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7566..7582)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7579..7601)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7590..7606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7614..7620,7621..7638,7639..7644))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7659..7680
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(7900..8559)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8474..8493
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(8560..8662)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(13400..13933)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(14122..14127,14128..14144,14145..14150))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     oriT            15150..15259
                     /note="incP origin of transfer"
     CDS             15292..15663
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 ttaattaatc gagctggttg ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa
       61 cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccggccgc cggcgttgtg
      121 gatacctcgc ggaaaacttg gccctcactg acagatgagg ggcggacgtt gacacttgag
      181 gggccgactc acccggcgcg gcgttgacag atgaggggca ggctcgattt cggccggcga
      241 cgtggagctg gccagcctcg caaatcggcg aaaacgcctg attttacgcg agtttcccac
      301 agatgatgtg gacaagcctg gggataagtg ccctgcggta ttgacacttg aggggcgcga
      361 ctactgacag atgaggggcg cgatccttga cacttgaggg gcagagtgct gacagatgag
      421 gggcgcacct attgacattt gaggggctgt ccacaggcag aaaatccagc atttgcaagg
      481 gtttccgccc gtttttcggc caccgctaac ctgtctttta acctgctttt aaaccaatat
      541 ttataaacct tgtttttaac cagggctgcg ccctgtgcgc gtgaccgcgc acgccgaagg
      601 ggggtgcccc cccttctcga accctcccgg tcgagtgagc gaggaagcac cagggaacag
      661 cacttatata ttctgcttac acacgatgcc tgaaaaaact tcccttgggg ttatccactt
      721 atccacgggg atatttttat aattattttt tttatagttt ttagatcttc ttttttagag
      781 cgccttgtag gcctttatcc atgctggttc tagagaaggt gttgtgacaa attgcccttt
      841 cagtgtgaca aatcaccctc aaatgacagt cctgtctgtg acaaattgcc cttaaccctg
      901 tgacaaattg ccctcagaag aagctgtttt ttcacaaagt tatccctgct tattgactct
      961 tttttattta gtgtgacaat ctaaaaactt gtcacacttc acatggatct gtcatggcgg
     1021 aaacagcggt tatcaatcac aagaaacgta aaaatagccc gcgaatcgtc cagtcaaacg
     1081 acctcactga ggcggcatat agtctctccc gggatcaaaa acgtatgctg tatctgttcg
     1141 ttgaccagat cagaaaatct gatggcaccc tacaggaaca tgacggtatc tgcgagatcc
     1201 atgttgctaa atatgctgaa atattcggat tgacctctgc ggaagccagt aaggatatac
     1261 ggcaggcatt gaagagtttc gcggggaagg aagtggtttt ttatcgccct gaagaggatg
     1321 ccggcgatga aaaaggctat gaatcttttc cttggtttat caaacgtgcg cacagtccat
     1381 ccagagggct ttacagtgta catatcaacc catatctcat tcccttcttt atcgggttac
     1441 agaaccggtt tacgcagttt cggcttagtg aaacaaaaga aatcaccaat ccgtatgcca
     1501 tgcgtttata cgaatccctg tgtcagtatc gtaagccgga tggctcaggc atcgtctctc
     1561 tgaaaatcga ctggatcata gagcgttacc agctgcctca aagttaccag cgtatgcctg
     1621 acttccgccg ccgcttcctg caggtctgtg ttaatgagat caacagcaga actccaatgc
     1681 gcctctcata cattgagaaa aagaaaggcc gccagacgac tcatatcgta ttttccttcc
     1741 gcgatatcac ttccatgacg acaggatagt ctgagggtta tctgtcacag atttgagggt
     1801 ggttcgtcac atttgttctg acctactgag ggtaatttgt cacagttttg ctgtttcctt
     1861 cagcctgcat ggattttctc atactttttg aactgtaatt tttaaggaag ccaaatttga
     1921 gggcagtttg tcacagttga tttccttctc tttcccttcg tcatgtgacc tgatatcggg
     1981 ggttagttcg tcatcattga tgagggttga ttatcacagt ttattactct gaattggcta
     2041 tccgcgtgtg tacctctacc tggagttttt cccacggtgg atatttcttc ttgcgctgag
     2101 cgtaagagct atctgacaga acagttcttc tttgcttcct cgccagttcg ctcgctatgc
     2161 tcggttacac ggctgcggcg agcatcacgt gctataaaaa taattataat ttaaattttt
     2221 taatataaat atataaatta aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt
     2281 tttgttttcc gaagatgtaa aagactctag ggggatcgcc aacaaatact accttttacc
     2341 ttgctcttcc tgctctcagg tattaatgcc gaattgtttc atcttgtctg tgtagaagac
     2401 cacacacgaa aatcctgtga ttttacattt tacttatcgt taatcgaatg tatatctatt
     2461 taatctgctt ttcttgtcta ataaatatat atgtaaagta cgctttttgt tgaaattttt
     2521 taaacctttg tttatttttt tttcttcatt ccgtaactct tctaccttct ttatttactt
     2581 tctaaaatcc aaatacaaaa cataaaaata aataaacaca gagtaaattc ccaaattatt
     2641 ccatcattaa aagatacgag gcgcgtgtaa gttacaggca agcgatccta gtacactcta
     2701 tattttttta tgcctcggta atgattttca tttttttttt ccacctagcg gatgactctt
     2761 tttttttctt agcgattggc attatcacat aatgaattat acattatata aagtaatgtg
     2821 atttcttcga agaatatact aaaaaatgag caggcaagat aaacgaaggc aaagatgaca
     2881 gagcagaaag ccctagtaaa gcgtattaca aatgaaacca agattcagat tgcgatctct
     2941 ttaaagggtg gtcccctagc gatagagcac tcgatcttcc cagaaaaaga ggcagaagca
     3001 gtagcagaac aggccacaca atcgcaagtg attaacgtcc acacaggtat agggtttctg
     3061 gaccatatga tacatgctct ggccaagcat tccggctggt cgctaatcgt tgagtgcatt
     3121 ggtgacttac acatagacga ccatcacacc actgaagact gcgggattgc tctcggtcaa
     3181 gcttttaaag aggccctagg ggccgtgcgt ggagtaaaaa ggtttggatc aggatttgcg
     3241 cctttggatg aggcactttc cagagcggtg gtagatcttt cgaacaggcc gtacgcagtt
     3301 gtcgaacttg gtttgcaaag ggagaaagta ggagatctct cttgcgagat gatcccgcat
     3361 tttcttgaaa gctttgcaga ggctagcaga attaccctcc acgttgattg tctgcgaggc
     3421 aagaatgatc atcaccgtag tgagagtgcg ttcaaggctc ttgcggttgc cataagagaa
     3481 gccacctcgc ccaatggtac caacgatgtt ccctccacca aaggtgttct tatgtagttt
     3541 tacacaggag tctggacttg acgctagtga taataagtga ctgaggtatg tgctcttctt
     3601 atctcctttt gtagtgttgc tcttatttta aacaactttg cggttttttg atgactttgc
     3661 gattttgttg ttgctttgca gtaaattgca agatttaata aaaaaacgca aagcaatgat
     3721 taaaggatgt tcagaatgaa actcatggaa acacttaacc agtgcataaa cgctggtcat
     3781 gaaatgacga aggctatcgc cattgcacag tttaatgatg acagcccgga agcgaggaaa
     3841 ataacccggc gctggagaat aggtgaagca gcggatttag ttggggtttc ttctcaggct
     3901 atcagagatg ccgagaaagc agggcgacta ccgcacccgg atatggaaat tcgaggacgg
     3961 gttgagcaac gtgttggtta tacaattgaa caaattaatc atatgcgtga tgtgtttggt
     4021 acgcgattgc gacgtgctga agacgtattt ccaccggtga tcggggttgc tgcccataaa
     4081 ggtggcgttt acaaaacctc agtttctgtt catcttgctc aggatctggc tctgaagggg
     4141 ctacgtgttt tgctcgtgga aggtaacgac ccccagggaa cagcctcaat gtatcacgga
     4201 tgggtaccag atcttcatat tcatgcagaa gacactctcc tgcctttcta tcttggggaa
     4261 aaggacgatg tcacttatgc aataaagccc acttgctggc cggggcttga cattattcct
     4321 tcctgtctgg ctctgcaccg tattgaaact gagttaatgg gcaaatttga tgaaggtaaa
     4381 ctgcccaccg atccacacct gatgctccga ctggccattg aaactgttgc tcatgactat
     4441 gatgtcatag ttattgacag cgcgcctaac ctgggtatcg gcacgattaa tgtcgtatgt
     4501 gctgctgatg tgctgattgt tcccacgcct gctgagttgt ttgactacac ctccgcactg
     4561 cagtttttcg atatgcttcg tgatctgctc aagaacgttg atcttaaagg gttcgagcct
     4621 gatgtacgta ttttgcttac caaatacagc aatagcaatg gctctcagtc cccgtggatg
     4681 gaggagcaaa ttcgggatgc ctggggaagc atggttctaa aaaatgttgt acgtgaaacg
     4741 gatgaagttg gtaaaggtca gatccggatg agaactgttt ttgaacaggc cattgatcaa
     4801 cgctcttcaa ctggtgcctg gagaaatgct ctttctattt gggaacctgt ctgcaatgaa
     4861 attttcgatc gtctgattaa accacgctgg gagattagat aatgaagcgt gcgcctgtta
     4921 ttccaaaaca tacgctcaat actcaaccgg ttgaagatac ttcgttatcg acaccagctg
     4981 ccccgatggt ggattcgtta attgcgcgcg taggagtaat ggctcgcggt aatgccatta
     5041 ctttgcctgt atgtggtcgg gatgtgaagt ttactcttga agtgctccgg ggtgatagtg
     5101 ttgagaagac ctctcgggta tggtcaggta atgaacgtga ccaggagctg cttactgagg
     5161 acgcactgga tgatctcatc ccttcttttc tactgactgg tcaacagaca ccggcgttcg
     5221 gtcgaagagt atctggtgtc atagaaattg ccgatgggag tcgccgtcgt aaagctgctg
     5281 cacttaccga aagtgattat cgtgttctgg ttggcgagct ggatgatgag cagatggctg
     5341 cattatccag attgggtaac gattatcgcc caacaagtgc ttatgaacgt ggtcagcgtt
     5401 atgcaagccg attgcagaat gaatttgctg gaaatatttc tgcgctggct gatgcggaaa
     5461 atatttcacg taagattatt acccgctgta tcaacaccgc caaattgcct aaatcagttg
     5521 ttgctctttt ttctcacccc ggtgaactat ctgcccggtc aggtgatgca cttcaaaaag
     5581 cctttacaga taaagaggaa ttacttaagc agcaggcatc taaccttcat gagcagaaaa
     5641 aagctggggt gatatttgaa gctgaagaag ttatcactct tttaacttct gtgcttaaaa
     5701 cgtcatctgc atcaagaact agtttaagct cacgacatca gtttgctcct ggagcgacag
     5761 tattgtataa gggcgataaa atggtgctta acctggacag gtctcgtgtt ccaactgagt
     5821 gtatagagaa aattgaggcc attcttaagg aacttgaaaa gccagcaccc tgatgcgacc
     5881 tcgttttagt ctacgtttat ctgtctttac ttaatgtcct ttgttacagg ccagaaagca
     5941 taactggcct gaatattctc tctgggccca ctgttccact tgtatcgtcg gtctgataat
     6001 cagactggga ccacggtccc actcgtatcg tcggtctgat tattagtctg ggaccacggt
     6061 cccactcgta tcgtcggtct gattattagt ctgggaccac ggtcccactc gtatcgtcgg
     6121 tctgataatc agactgggac cacggtccca ctcgtatcgt cggtctgatt attagtctgg
     6181 gaccatggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg gtcccactcg
     6241 tatcgtcggt ctgattatta gtctggaacc acggtcccac tcgtatcgtc ggtctgatta
     6301 ttagtctggg accacggtcc cactcgtatc gtcggtctga ttattagtct gggaccacga
     6361 tcccactcgt gttgtcggtc tgattatcgg tctgggacca cggtcccact tgtattgtcg
     6421 atcagactat cagcgtgaga ctacgattcc atcaatgcct gtcaagggca agtattgaca
     6481 tgtcgtcgta acctgtagaa cggagtaacc tcggtgtgcg gttgtatgcc tgctgtggat
     6541 tgctgctgtg tcctgcttat ccacaacatt ttgcgcacgg ttatgtggac aaaatacctg
     6601 gttacccagg ccgtgccggc acgttaaccg ggctgcatcc gatgcaagtg tgtcgctgtc
     6661 gacgagctcg cgagctcgga catgaggttg ccccgtattc agtgtcgctg atttgtattg
     6721 tctgaagttg tttttacgtt aagttgatgc agatcaatta atacgatacc tgcgtcataa
     6781 ttgattattt gacgtggttt gatggcctcc acgcacgttg tgatatgtag atgataatca
     6841 ttatcacttt acgggtcctt tccggtgatc cgacaggtta cggggcggcg acctcgcggg
     6901 ttttcgctat ttatgaaaat tttccggttt aaggcgtttc cgttcttctt cgtcataact
     6961 taatgttttt atttaaaata ccctctgaaa agaaaggaaa cgacaggtgc tgaaagcgag
     7021 ctttttggcc tctgtcgttt cctttctctg tttttgtccg tggaatgaac aatggaagtc
     7081 cgagctcatc gctaataact tcgtatagca tacattatac gaagttatat tcgatgcggc
     7141 cgcaaggggt tcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     7201 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccaca cagatgcgta
     7261 aggagaaaat accgcatcag gcgccattcg ccattcagct gcgcaactgt tgggaagggc
     7321 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     7381 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     7441 aattgtaata cgactcacta tagggcgaat tcgagctcgg tacccgggga tcctctagag
     7501 tcgacctgca ggcatgcaag cttgagtatt ctatagtctc acctaaatag cttggcgtaa
     7561 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7621 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7681 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7741 tgaatcggcc aacgcgaacc ccttgcggcc gcccgggccg tcgaccaatt ctcatgtttg
     7801 acagcttatc atcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca
     7861 accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc
     7921 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
     7981 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     8041 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     8101 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa
     8161 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     8221 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
     8281 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
     8341 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     8401 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     8461 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     8521 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     8581 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     8641 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
     8701 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
     8761 ttattcgcga taagctcatg gagcggcgta accgtcgcac aggaaggaca gagaaagcgc
     8821 ggatctggga agtgacggac agaacggtca ggacctggat tggggaggcg gttgccgccg
     8881 ctgctgctga cggtgtgacg ttctctgttc cggtcacacc acatacgttc cgccattcct
     8941 atgcgatgca catgctgtat gccggtatac cgctgaaagt tctgcaaagc ctgatgggac
     9001 ataagtccat cagttcaacg gaagtctaca cgaaggtttt tgcgctggat gtggctgccc
     9061 ggcaccgggt gcagtttgcg atgccggagt ctgatgcggt tgcgatgctg aaacaattat
     9121 cctgagaata aatgccttgg cctttatatg gaaatgtgga actgagtgga tatgctgttt
     9181 ttgtctgtta aacagagaag ctggctgtta tccactgaga agcgaacgaa acagtcggga
     9241 aaatctccca ttatcgtaga gatccgcatt attaatctca ggagcctgtg tagcgtttat
     9301 aggaagtagt gttctgtcat gatgcctgca agcggtaacg aaaacgattt gaatatgcct
     9361 tcaggaacaa tagaaatctt cgtgcggtgt tacgttgaag tggagcggat tatgtcagca
     9421 atggacagaa caacctaatg aacacagaac catgatgtgg tctgtccttt tacagccagt
     9481 agtgctcgcc gcagtcgagc gacagggcga agcccgttta aaccatgatt acgccaagct
     9541 cgcgaggcct cgagatctat cgatgcatgc catggtaccc gggagctcga attctagaag
     9601 cttctgcaga cgcgtcgacg tcatatggat ccgattcgac ctgcaggcat gcaagctgta
     9661 cgcctcagct accattcttg cagccgcgtt cagggtcttg acgtgttctt tgtatctatc
     9721 agcaaactga gaacccaatt ctggatcatc cttgtaacgc caactctccg accattccga
     9781 aggaacgcct tgtagtcctc ctgagccgtt agggtatgtc tttagtccaa tcaaatggga
     9841 catttgcaga caggaacgca caagtgtctc acgtcgcttc tctatttcaa tatctaaaaa
     9901 ttcttcactc ttatcgttgc ttgacatatt aaaactgtct aactgatcca gttcacttat
     9961 atggaaagta tcactaaagt catggcttct cagaaattca atacttccca ttgatgggga
    10021 ccggttaacc aaatcgataa aaatttttct gtcatgtttc agataagttt ttggcgcgtt
    10081 gattttataa taaagccgcc attcttgtgc caacaacgcc acgaaaagag ccacagcagc
    10141 agcggccaca tcccattgaa aatctttcgc cagaccaacc gccaggagaa ccaagccgaa
    10201 atagttagcc cagcgaacta aaaaactatc tataaacgta ttaagaaacg ttcgggtcat
    10261 atttccgtga atcatatcag gacgtaaatt tgcacatgac gcatttccgg gggcttttat
    10321 ccctcggact tcttttcagg ccccttctcg gcctttctgt gaccctgttt tttcaggccg
    10381 cgaactgcgc ctggtcgggc ctgaccccca cacgctggcc ttcgtactca cgcagcctgc
    10441 ccagcaggtc ggccagcgcg gcattgacca cggcggcgag gttgcggggc ggcgttccgc
    10501 ctgcggtctg gtcgtgcttc acgtcgcgca gcacgcgcac cagcaccgcg cccacgtcgt
    10561 cggacacgtc ccggccagcg tcggcggcgc gggtgatgtt ccaaatgagt ttccgccaga
    10621 agcccagcga gtcggccccg tccccgaaag ctgccgctag cgcccgcgcc tgcctgtcca
    10681 cgatctcggc gcggtaggcc gggcgggttt cccggtgggc gtcggcaagc tcccacacga
    10741 cgttttcagc gagggagggc gcgggccgga cagtcatgtt atcggagggg gagggaagag
    10801 cggattttat cgcccaggtg ctcacaactt cgtatgtcac ggcaaccggg gcgctttttt
    10861 cgcgcagttc cccgcctgtc acatcctcac agggttcttg actctccggc acgctcttag
    10921 ggagagggtg gagcatgttg tagacggtgc gcccggcctt cgcgtcggcg ttcaggtcgc
    10981 gccagttccg gccccaatcg tcatgcatca ggcggacata tcccgccttc ccttccagca
    11041 cgcgcctcgg cttgagcgtc acggcccaca gcgtcccggt tgccacagac tcgccgcgca
    11101 ggtcgcccat gtgggcatca caggccacca gcccgacccg gcgcagatat tgaaggttct
    11161 cgtagaaggc ggacttcttc agacccacat gcaccatgag cagctcggcg ggcaggtgga
    11221 acacagcgcg ggtgacgtgt tccgcatagc cgcaggcgcg ggccacgtcc agcgccaccg
    11281 agaacagggc gcggaagatg cgccgggccg actcgcggca gggggcgtca tctaggcagg
    11341 cggtgagggt ctgcaccagt tcggcgcgtg tggcgatggt caacggcgcg ggcgcgggct
    11401 gggactgctg ggcgatggtg ggcacgggcg gcggcgagat ggaccgggcc gagctggaaa
    11461 tttcccgtgt gagcgcaggt ttaggcgcat ggttaccttc ctgcagtgaa atctgcgaaa
    11521 tcaggcccgc ttcgaggagt cggaccatga atggattgtg tctcaagaaa aaagcctccc
    11581 ctcttgtcag aggggagggt cccggtctac catgctctca gcgacgagat agctggggtt
    11641 tgtgaccctg cactctgaca aagccctcac cgaaaggtgg gggtttagtc atttggccta
    11701 gtaaagcaga acagaggcga tttgtcacgc aaaggcccgc cgcccgaggg gaaacaggcg
    11761 gcgggccaat ggctcggcgt tgttagctgg gggtcgcacc agggaagcac agaagccctt
    11821 ccctgagcag ggtttcaccg tgtggtgtcc cttcgtcctt gaaggcgaac tggtaggcca
    11881 ggccgtaatg ctcctgcacc atcttttgct catccaccgc gacaaagaaa cggcgggtgt
    11941 tcgccgggct gctgagggtc tgccactcgc cggacgcgcc caccgcatag gcgtctccgg
    12001 ctccacattc ctcggcctgc tgatactgat acttcgtcca cagcatcacc gccttatcgg
    12061 gcacctgcgc gaagctcacc agcgccccga accgcccggc gctcgtggtg aaggtggacg
    12121 actgcaccat gtcggacagt tccagcgtgg caggcgcggg cgtgttcgtc ggcgcgtaca
    12181 ccgtccccgc ctcgcacacg accagcgggg cggcccccgt gccgaactcg gcggcgctgc
    12241 cgtttgccag cgtgagcgcc gaagacgcca ccgcgcaggt acccatgggt gccgaccacc
    12301 agcaggtcca cgccgtgcgc cgcctcggtg agcacctcga ttggacggcc cctcagggtc
    12361 tgaaccctcc cctgcacgcc cgcctccccg atgagtgtct gcgccgtctg ctgcgcctgc
    12421 cgggcattct gctccagcat ctcccggacg tagtcgggat tcagtcccgc ccactgaacg
    12481 aagccgctga ccggcggctc ctcgatcaca gtcacgacct ccagctcggc gcctgccgcc
    12541 ttcccgagcg ttatggcctg cttcaaggcg cgctggctgg gctcacttcc gtcaaaggcc
    12601 accacgaatc gcatccgtcc tcctccctga ccagtcaggt tcagcaagca gggtaagcct
    12661 ctcccagcac tccggggctt cacacggcct tgggtctttt ctgcataccc atgagcctca
    12721 gtacccgaca gtttccggca gggcttgaaa aagttcggct gacacgcgag aactggacgc
    12781 gtctcccgat gtcctggcca agttgacccg ctgcttcacc gcgcacttcc cggagttccg
    12841 caagaactag gtcgagctgc tctccctcat ggtcctcgcc ctccttaggg gcaaggacgt
    12901 ccggcatgct aaactcgccg cgcgcttccc cggaagcgcg cacaccgcct ccgtcatccg
    12961 gcgggtggaa cgcttcttcg accgtcatcc tcttcggcca gctgatgtcg cccgggtcgt
    13021 tctgacgctc cttcccgccg cgcagccacg cgaatttatc cttgaccgga ccaactggaa
    13081 gtatgggcag acggacgtga acgtcttgtt gctggccgtc atttggcggg acgtcgccat
    13141 ccccctgctc tacgagttgc tgccccatgg gggcagcagc gacaccgaga ttcggcacac
    13201 cctgatggac gatgccctgt gcctgctgtc cgccgctgac atccgggtgc tgtatgccga
    13261 ccgcgaattc ccagcttgag taggacaaat ccgccgagct tcgacgagat tttcaggagc
    13321 taaggaagct aaagcggcgt tgtgacaatt taccgaacaa ctccgcggcc gggaagccga
    13381 tctcggcttg aacgaattgt taggtggcgg tacttgggtc gatatcaaag tgcatcactt
    13441 cttcccgtat gcccaacttt gtatagagag ccactgcggg atcgtcaccg taatctgctt
    13501 gcacgtagat cacataagca ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg
    13561 cggtggcaat gccctgcctc cggtgctctc cggagactgc gagatcatag atatagatct
    13621 cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc gagagcgcca acaaccgctt
    13681 cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg gagcaagttc ccgaggtaat
    13741 cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat
    13801 caagagcagc ccgcatggat ttgacttggt cagggccgag cctacatgtg cgaatgatgc
    13861 ccatacttga gccacctaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc
    13921 tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct
    13981 tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa
    14041 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag
    14101 cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa ctgggttcgt
    14161 gccttcatcc gtttccacgg tgtgcgtcgc gagggcctga gggccatgga gaccgagggc
    14221 cctggacatt gagaatgatt ctcaatatgg tgcagggagc ttcgggcctc ttgccgcgca
    14281 gcagagccag cgaggcgaag gagagtgtat ggaaaaaaag attactggtt acaccaccgt
    14341 ggacattagt cagtggcatc gtaaggagca tttcgaagcg tttcaaagcg tggcacagtg
    14401 cacgtacaac cagaccgttc aactcgacat caccgccttc ctgaagaccg tgaagaaaaa
    14461 caagcacaag ttttaccccg cctttattca catccttgcc cgcttgatga atgctcaccc
    14521 tgagctccgc atggccatga aagacgggga gctggtcatc tgggatagcg tgcacccgtg
    14581 ttacaccgtc ttccacgagc agaccgagac gttctcgtct ctctggtcgg agtatcacga
    14641 cgacttccgg cagttcctgc acatctacag ccaggacgtc gcgtgctacg gcgagaacct
    14701 cgcgtacttc ccgaagggct ttatcgaaaa tatgtttttt gtgtccgcca acccctgggt
    14761 gagcttcacc agcttcgacc tgaacgtcgc caacatggac aactttttcg ccccggtctt
    14821 cacgatggga aagtattaca cgcagggcga caaagtgctg atgcccctgg cgatccaggt
    14881 gcaccacgcg gtgtgcgacg ggttccacgt gggccgcatg ctgaacgaac tgcagcagta
    14941 ctgcgacgaa tggcagggcg gcgcgtgata ggcagaaaaa atccccccgg tggcaatccg
    15001 gggggttttt ttaaatcttc cgctgcataa ccctgcttcg gggtcattat agcgattttt
    15061 tcggtatatc catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac
    15121 tttccttggt gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg
    15181 agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct
    15241 gctctgcgag gctggccggc taccgccggc gtaacagatg agggcaagcg gatggctgat
    15301 gaaaccaagc caaccaggaa gggcagccca cctatcaagg tgtactgcct tccagacgaa
    15361 cgaagagcga ttgaggaaaa ggcggcggcg gccggcatga gcctgtcggc ctacctgctg
    15421 gccgtcggcc agggctacaa aatcacgggc gtcgtggact atgagcacgt ccgcgagctg
    15481 gcccgcatca atggcgacct gggccgcctg ggcggcctgc tgaaactctg gctcaccgac
    15541 gacccgcgca cggcgcggtt cggtgatgcc acgatcctcg ccctgctggc gaagatcgaa
    15601 gagaagcagg acgagcttgg caaggtcatg atgggcgtgg tccgcccgag ggcagagcca
    15661 tgactttttt agccgctaaa acggccgggg ggtgcgcgtg attgccaagc acgtccccat
    15721 gcgctccatc aagaagaggc acttcgagct gtaagtacat caccgacgag caaggcaaga
    15781 cgatc
//
