LOCUS       sequence-360443-       11845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for GFAP::Opto-vTrap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11845)
  AUTHORS   Won J, Pankratov Y, Jang MW, Kim S, Ju YH, Lee S, Lee SE, Kim A, 
            Park S, Lee CJ, Heo WD
  TITLE     Opto-vTrap, an optogenetic trap for reversible inhibition of 
            vesicular release, synaptic transmission, and behavior.
  JOURNAL   Neuron. 2022 Feb 2;110(3):423-435.e4. doi: 
            10.1016/j.neuron.2021.11.003. Epub 2021 Nov 30.
  PUBMED    34852235
REFERENCE   2  (bases 1 to 11845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2021.11.003"; journalName: "Neuron"; date: 
            "2022-02-2- 2"; volume: "110"; issue: "3"; pages: "423-435.e4"
COMMENT     Sequence Label: pLenti-GFAP-CRY2PHR-P2A-CIBN-mCherry-VAMP2
FEATURES             Location/Qualifiers
     source          1..11845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             4823..4879
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4880..5389
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     regulatory      5402..5411
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5408..6115
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5553..5571)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5821..5840
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6537..7125
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6590..6610)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7008..7019)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7128..7144)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7129..7145)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7650..7830
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7856..7873)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7862..8086
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8132..8560
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8219..8238)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8429..8450
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8569..8589)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8574..8903
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8754..8889
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8816..8835
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8951..8998
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9017..9391
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9521..9642
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9558..9577)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9612..9631
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9691..9707)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9691..9707)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9704..9726)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9715..9731
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9739..9745,9746..9763,9764..9769))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9784..9805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9922..9939)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10093..10681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10173..10192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10852..11643,11644..11712))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11713..11817)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aagatctaac atatcctggt gtggagtagg ggacgctgct
     2641 ctgacagagg ctcgggggcc tgagctggct ctgtgagctg gggaggaggc agacagccag
     2701 gccttgtctg caagcagacc tggcagcatt gggctggccg ccccccaggg cctcctcttc
     2761 atgcccagtg aatgactcac cttggcacag acacaatgtt cggggtgggc acagtgcctg
     2821 cttcccgccg caccccagcc cccctcaaat gccttccgag aagcccattg agcagggggc
     2881 ttgcattgca ccccagcctg acagcctggc atcttgggat aaaagcagca cagcccccta
     2941 ggggctgccc ttgctgtgtg gcgccaccgg cggtggagaa caaggctcta ttcagcctgt
     3001 gcccaggaaa ggggatcagg ggatgcccag gcatggacag tgggtggcag ggggggagag
     3061 gagggctgtc tgcttcccag aagtccaagg acacaaatgg gtgaggggag agctctcccc
     3121 atagctgggc tgcggcccaa ccccaccccc tcaggctatg ccagggggtg ttgccagggg
     3181 cacccgggca tcgccagtct agcccactcc ttcataaagc cctcgcatcc caggagcgag
     3241 cagagccaga gcaggttgga gaggagacgc atcacctccg ctgctcgcgg ggatccttcg
     3301 aaaccggtgc tagcccacca tgaagatgga caaaaagacc atcgtctggt ttcggagaga
     3361 tttgagaata gaagataatc ccgcgctcgc cgccgcggcc cacgagggtt ccgtcttccc
     3421 cgttttcatt tggtgtcctg aagaagaagg ccagttttat cccggaaggg cctctaggtg
     3481 gtggatgaag caaagtctgg cccatcttag ccagtcactg aaagcactgg gcagtgatct
     3541 taccctgatc aagacacaca ataccatctc tgccatcctc gactgcatca gggtgaccgg
     3601 cgcaacgaaa gtcgtgttta accacctgta cgatccagtt agtctggtgc gcgaccacac
     3661 tgtgaaggag aagctggtgg aacgggggat cagtgtgcag agctacaacg gggaccttct
     3721 gtacgagcca tgggagatct attgcgagaa agggaaaccg ttcacctcct tcaacagtta
     3781 ctggaagaaa tgtttggata tgtcaataga gtccgttatg ttgccccctc cctggagact
     3841 gatgccgatt actgctgctg cagaggccat ctgggcctgc tccatcgagg aactcggtct
     3901 ggaaaatgaa gcagaaaagc caagcaatgc acttctcact agagcctgga gccccggctg
     3961 gtctaatgcc gacaagctgc ttaacgagtt catcgaaaaa caactgattg actacgcgaa
     4021 gaactccaag aaagtggtag gtaactcaac tagcttgctc tctccatatc tccattttgg
     4081 cgagatttct gtccgccatg tatttcagtg cgctcggatg aaacagatta tctgggctcg
     4141 cgataaaaac agcgaaggcg aagaaagcgc cgatctgttc ctgcgaggga tcggacttcg
     4201 ggaatactcc cggtatatat gtttcaactt tccattcaca cacgagcaga gtctgttgtc
     4261 ccacctcagg ttcttcccct gggacgccga tgtcgacaaa ttcaaggcat ggagacaggg
     4321 aaggacaggg tacccactcg tggatgctgg catgagagag ctctgggcta caggctggat
     4381 gcacaaccgc atccgggtaa tcgtgtcctc atttgctgtc aagtttctgc tcctgccttg
     4441 gaaatgggga atgaagtact tttgggatac ccttctcgac gccgacttgg agtgtgacat
     4501 tctgggatgg caatatatta gcgggtcaat tcctgacggc catgagttgg acaggttgga
     4561 caatccggcc ttgcagggag ctaagtatga tcccgaagga gagtatattc gacagtggct
     4621 ccccgagctg gcccgacttc ctacggagtg gattcaccat ccttgggacg caccactgac
     4681 agtgctcaag gcaagcgggg tggagctggg caccaattac gctaagccta tagttgatat
     4741 agatacagca cgcgagctgc tggctaaagc gatctctcgc actcgggagg cgcagattat
     4801 gatcggtgct gccggcagcg gcgccacgaa cttctctctg ttaaagcaag caggagacgt
     4861 ggaagaaaac cccggtccta tgaatggagc tataggaggt gaccttttgc tcaattttcc
     4921 tgacatgtcg gtcctagagc gccaaagggc tcacctcaag tacctcaatc ccacctttga
     4981 ttctcctctc gccggcttct ttgccgattc ttcaatgatt accggcggcg agatggacag
     5041 ctatctttcg actgccggtt tgaatcttcc gatgatgtac ggtgagacga cggtggaagg
     5101 tgattcaaga ctctcaattt cgccggaaac gacgcttggg actggaaatt tcaaggcagc
     5161 gaagtttgat acagagacta aggattgtaa tgaggcggcg aagaagatga cgatgaacag
     5221 agatgaccta gtagaagaag gagaagaaga gaagtcgaaa ataacagagc aaaacaatgg
     5281 gagcacaaaa agcatcaaga agatgaaaca caaagccaag aaagaagaga acaatttctc
     5341 taatgattca tctaaagtga cgaaggaatt ggagaaaacg gattatattc atgtaccggt
     5401 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     5461 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     5521 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     5581 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     5641 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     5701 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     5761 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     5821 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     5881 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     5941 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     6001 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     6061 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagggcag
     6121 tggttccgga ctcagatctc gagctatgtc ggctaccgct gccaccgtcc cgcctgccgc
     6181 cccggccggc gagggtggcc cccctgcacc tcctccaaac cttactagta acaggagact
     6241 gcagcagacc caggcccagg tggatgaggt ggtggacatc atgagggtga atgtggacaa
     6301 ggtcctggag cgggaccaga agttgtcgga gctggatgac cgtgcagatg ccctccaggc
     6361 aggggcctcc cagtttgaaa caagtgcagc caagctcaag cgcaaatact ggtggaaaaa
     6421 cctcaagatg atgatcatct tgggagtgat ctgcgccatc atcctcatca tcatcatagt
     6481 ttacttcagc tcttaaggcg cgcccctgca gggaattcga tatcaagctt atcgataatc
     6541 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6601 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6661 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6721 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6781 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6841 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6901 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     6961 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7021 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     7081 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     7141 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     7201 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     7261 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7321 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     7381 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
     7441 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
     7501 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
     7561 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
     7621 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
     7681 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7741 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7801 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
     7861 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     7921 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     7981 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     8041 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     8101 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     8161 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     8221 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     8281 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     8341 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     8401 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     8461 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     8521 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     8581 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     8641 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     8701 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     8761 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8821 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     8881 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     8941 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     9001 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     9061 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     9121 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     9181 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     9241 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     9301 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     9361 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     9421 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     9481 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     9541 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9601 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     9661 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     9721 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9781 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9841 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     9901 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     9961 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    10021 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    10081 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    10141 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    10201 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10261 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    10321 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    10381 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10441 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10501 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    10561 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    10621 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    10681 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10741 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    10801 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    10861 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    10921 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    10981 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    11041 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    11101 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    11161 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    11221 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    11281 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    11341 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    11401 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    11461 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11521 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    11581 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    11641 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    11701 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    11761 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    11821 atttccccga aaagtgccac ctgac
//
