LOCUS       sequence-360471-       10899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmXRN1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Haas G, Braun JE, Igreja C, Tritschler F, Nishihara T, Izaurralde E
  TITLE     HPat provides a link between deadenylation and decapping in metazoa.
  JOURNAL   J Cell Biol. 2010 Apr 19. 189(2):289-302.
  PUBMED    20404111
REFERENCE   2  (bases 1 to 10899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2010 Apr 19. 189(2):289-302."
COMMENT     Sequence Label: pAc5.1B-EGFP-DmXRN1_A
FEATURES             Location/Qualifiers
     source          1..10899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2575..2596)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2836..2855)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3183..3204
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8150..8191
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8201..8218
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8241..8258)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8291..8425
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8315..8334)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8369..8388
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(9026..9614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9106..9125)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9785..10576,10577..10645))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10408..10427
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10646..10750)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10818..10836
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtaccatca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2581 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2641 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2701 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     2761 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2821 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2881 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2941 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     3001 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     3061 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     3121 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     3181 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     3241 tacaagccaa gcttgaattc tgcagatatc cagcacagtg gcggccgcat gggcgttccc
     3301 aagttctttc gctacatcag cgagcgatat ccgtgcctca gcgaattggc gcgggagcac
     3361 tgcatacccg agttcgacaa tctgtacttg gacatgaacg gcattgtcca caactgctcg
     3421 catccggacg acaacaacat acactttcac ctggaggagg agcagatatt ccaggaaatc
     3481 ttcaactacg tagacaagct gttctacctg atcaagccgc agcgcctctt cttcctttca
     3541 gttgacgggg tggctccacg ggccaagatg aaccagcagc gcagccggcg attccggacg
     3601 gcgcgcgagg cggagcagca ggaggccaag gcggcccagc gaggcgagtt gcgagagcac
     3661 gagcgcttcg acagcaactg cattacgccg ggcaccgaat tcatggtgcg tctgcaggag
     3721 ggactgcgcg cctttctcaa aacgaagatt tcgacggatc cgctgtggca gcgttgcacc
     3781 gtaatcctaa gtggccagga ggcgcccggc gagggcgagc acaagatcat ggactacatt
     3841 cggtacatga agacgcagcc ggactatgat cccaatacac gacactgcct ttacggcctg
     3901 gacgccgatc tgatcattct gggcctctgc acccacgagc tgcacttcgt ggtgctgcgc
     3961 gaggaggtca agttcgggcg caacgtcaag cgcacctcgg tggaggagac gcgcttcttc
     4021 ctgctgcacc tgggtctgct gcgcgagtac ctcgagctgg agttcgacgc cctgagaaca
     4081 gacgagcaca aattggacat tgcgcagctt atcgacgact gggtgctgat gggctttttg
     4141 gtcggcaacg acttcattcc ccacctgccg tgcctgcaca tttcatcaaa tgcactgccg
     4201 ctgctctacc gcacctacat cggcatttac ccgaccctcg gcggcaacat caacgagaac
     4261 ggcaagctca acctgcgccg cttgcagatt ttcatatcgg ccctcaccga ggtggagctg
     4321 gatcacttca aggagcacgc cgacgacttg aagtacatga acaacaaatc agaggcgttc
     4381 gacatggacg tcggcgagat caccgagtct caaaacctcg actccgatct cggagccctc
     4441 atcaacaaga gcatgctgct ctacgatgac gacagcgagg aggactgctc cgacgagaac
     4501 gcggttcttc tgaaggagtt ccagaactac aagcgaaact tctaccgcaa caagttcaag
     4561 cgtgatccga atgatgaact gatcgaggaa ctgtgccacc actacgtcaa tgcgctgcag
     4621 tgggtgctcg actactacta ccgtggcgtg cagtcctggg actggtacta tcccttccac
     4681 tacactccat tcatcagcga cctcaagaac atcgagcagg tggagatcgc cttccacatg
     4741 ggcacgccct ttctgccatt ccagcagctt ctggcggtac tgccggcggc cagtgcaaag
     4801 cttctccctg tcgcctacca cgacctcatg ctgctgccga ccagcccgct ggccgagttc
     4861 tatccactgg agttcgagag cgacctcaat ggcaagaaac acgattggga ggcggttgtg
     4921 ctgataccct tcatcgatga gggtcggctg ctggccgcta tgctgccatg cgaggctcag
     4981 ctgtcgctgg aggagcgcga acgcaaccga cacggtccca tgtatgtgta caaatacagt
     5041 acggtggcgc agggtcccat gccagcgtat ccaccgctga gggctcttcc cgtgctctac
     5101 tgcacggagg tggccaagtg gtcgcacgaa atcgcagtca atctgccgta cagcgtgtgc
     5161 atcgagctac caaatgcggc gagaaccgtt ttcttcccgg gatttcccac catgcagcat
     5221 ctgcccttcg atttcgagct gcgcaacgac cgcgtcaagg tgttcgaaca ggtcagtcgc
     5281 aaccagaaca ttgtgctaaa accacgcaag cgccagctgg aggacacact gacggcggtg
     5341 gcctcccagt acctcgggaa ggtgatccac gtcggctggc cgcatctcgt caaggcgata
     5401 gtggtgcgcg tggccacgcg agatcagcgc gtcgacagcg agggaatcac gctaaacgat
     5461 tcgcggcgtt tcgactcgga atgcaaggca ctgcaggaac actttatcaa tcgtatggga
     5521 atacagtttg ccaactatga tgtgctcgtc tatgtgcgca cctttgccgg caattcgacg
     5581 gagttccgcg acaagggggc attgatggtg cgcgactcgt ggagcagctc cgtcaccggc
     5641 tatccagccc agggcgtggt ggccgatctg actgtgtggg aaagaatgcg caagaatttc
     5701 ctcaacgtgg agcactactt ccccgttggc agcacgatct tcttgatcac ggatccgtat
     5761 tacggcagcg agggaactgt gcaggaccca cggctggcct acaccaacgg ccggattcaa
     5821 gtcagcatca tggtgcgacc ggaaccgaaa gtaaatgcgg cacgtcagct gcaggaagag
     5881 cgggaccggg actatctgag caccttccag gtctgcaact tgctccgaat cagtggccgc
     5941 accctgggac gcctaagtgg caccgtgtgg gtggtgctcg gtccgcggcg tcagaagatg
     6001 gaaaacgtga ccaagcacaa cattggcctg cagctcaagt atccgcggca gaacgaggag
     6061 cgtgccggat actgcttccg cacgaacaac cagtggtact actccagcct ggccgtggat
     6121 ctgatgcgta actattgcca gcgttaccca gacgttatcg acttctttgg cgactccaac
     6181 gatcgcgccg agttcgtatt cgaacaggac gtgttcccca atgcggttgg ccatcgccgc
     6241 gtcgaggaac tggccaactg ggtgcgccag cagccgcaca tgaaagtgga gcgcatctct
     6301 tgcggctcca agacggtctg ccgcgagacc atcgaactgc tgatagccgc cgtcgacgac
     6361 ctgcgctcac tgcccgtcaa gcacgttaaa ctgcaggtga agccgcactt gctcatcaag
     6421 cccaatgtca cactgccaga tgtctaccga tcaaagcgcc cggtgcgtct cttcgatcgc
     6481 gtcgtaatcg tacgcaccat ttacatggtg cccgtgggca ctaagggcac cgtgattggc
     6541 attcatcccg ttacggaccc caacccggtc cgcctggagt gcgtccatgc tgtcgacacg
     6601 ttctgcaagg tgctgttcga cagccccgtt ccgaactgca acaacatcca tggcatcgcc
     6661 gaagaccgag tttacaaggt gcccgaaatt gccctggtga tcatcaagac ggatgaggaa
     6721 ggaaagaagc agaacgactg cgaactgccg gtgagggatc ctcagccgaa ccaggcacag
     6781 gacgagccag tccgcgcgac cagcagtcgc tatgtgaccg ccgcagggtc cacctcggtc
     6841 ccgatcacga tgaagaccca aattagcgac gaattcgtca agacgcgtag cgatccgata
     6901 gcccgtacgg attcgtacaa gccatcgtcg gagccaaaac cggtgccggt gccggagcag
     6961 attaccaact ggcgggaacg cgtcagtacg ccaacaaaca aaccgcagcc tgcgccaaat
     7021 aattggcgca taaatcggtc ttcatcacgt caacagggcg gtagtatttt tgttgctccg
     7081 cccacaaaaa ctcctgatgc agctgcaagc actgcttcca ctgcattcac cgcagcctcg
     7141 tccgcaacgc tgactcctct ggatcagacc ttggcgctca tgtcggtttt gggcgtgggt
     7201 gaggaccaga gctcgccgcc actccaggag gcggtgcagc agcaacgtcc gccactactt
     7261 caacagcaaa gagctccgtt tcccggacaa atgcccaatc tacccaagcc accgctcttc
     7321 tggcagcaag aggcgcagaa gcaggaggcg ctgcagcagg aggcgcagca gcaggaggcg
     7381 cagaagaagc agcagcaggc gcatgcgcaa atggagcctg aacgtatcaa tagccagcat
     7441 ttctacagaa gcggccagac aggagcggcg ttgaatcagc cgccattggg tgctccatca
     7501 aaaaggcagt ggcatgagtg ggtgcacccg cgcatgcagc acgccaacgc attccacgct
     7561 ggcgtcaaca atggatatca gatgcgtccc aaaaagaata tagctgcgca gagcaccttt
     7621 aacaacaacg tgcacatgca cctccagcaa ccatattacc cgaatcagca gcagcagcag
     7681 cagcagcagc agccactcca gctgacggag atcaacaatg cgccaccgcg atactcaacg
     7741 atccaagatt ttgtgcccat acaggcgtac cgtcccaaga aattaaatcg cgtccaacca
     7801 gccggtcgtc aggacgtcga tgccaccaag aatcctagtc gttcgcctgt gttgcagcaa
     7861 ccgacgaacg agactataga cacaaaggcc agttctagtc ttccggtaca atcagcaggc
     7921 gaacaggtga ttggactgat gcaaacgctt gaaattaagc cggctgcgtc gcagtccgaa
     7981 tctgatgggg tctcaacagg cagtgcgaac gcgcccacag caacgacatc atcccaggcg
     8041 gtcaaccgaa ggaagcatcg agtgcctcgc atcggagcca agttcgactt ggaatacata
     8101 ctgcccgact ccccgcatcc aacttaatct agagggcccg cggttcgaag gtaagcctat
     8161 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg
     8221 agtttaaacc cgctgatcag cctcgactgt gccttctaag gcctgagctc gctgatcagc
     8281 ctcgatcgag gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     8341 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     8401 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     8461 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     8521 tgattatgat cagtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     8581 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     8641 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg
     8701 cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct
     8761 tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg
     8821 cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc
     8881 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
     8941 ccaggcaggt agatgacgac catcagggac agcttcaagg ccagcaaaag gccaggaacc
     9001 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9061 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9121 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9181 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9241 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9301 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9361 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9421 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     9481 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9541 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9601 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9661 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9721 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9781 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9841 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9901 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9961 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10021 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10081 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10141 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10201 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10261 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10321 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10381 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
    10441 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10501 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10561 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    10621 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    10681 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10741 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
    10801 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattaattc
    10861 tatattctaa aaacacaaat gatacttcta aaaaaaaat
//
