LOCUS       sequence-360485-       10235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK 
            I1171N/D1203N mutation in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Shiba-Ishii A, Johnson TW, Dagogo-Jack I, Mino-Kenudson M, Johnson 
            TR, Wei P, Weinrich SL, McTigue MA, Walcott MA, Nguyen-Phuong L, 
            Dionne K, Acker A, Kiedrowski LA, Do A, Peterson JL, Barth JL, Yeap 
            BY, Gainor JF, Lin JJ, Yoda S, Hata AN
  TITLE     Analysis of lorlatinib analogs reveals a roadmap for targeting 
            diverse compound resistance mutations in ALK-positive lung cancer.
  JOURNAL   Nat Cancer. 2022 Jun 20. pii: 10.1038/s43018-022-00399-6. doi: 
            10.1038/s43018-022-00399-6.
  PUBMED    35726063
REFERENCE   2  (bases 1 to 10235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cancer.
            2022 Jun 20. pii: 10.1038/s43018-022-00399-6. doi: 
            10.1038/s43018-022-00399-6."
COMMENT     Sequence Label: pLenti-EML4-ALK variant 3a I1171N/D1203N
FEATURES             Location/Qualifiers
     source          1..10235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..219
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     170..190
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     216..240
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     315..334
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        315..333
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    363..387
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2782..2806)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2838..2861
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2877..2903
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2877..2903
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2910..3476
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2926..3478
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3070..3087)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3297..3316
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3490..4089
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3490..3509)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3986..4006
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4106..4694
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4159..4179)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4577..4588)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4769..5002
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5121..5139)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5207..5311
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5312..5380,5381..6172)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5530..5549)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6343..6931
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6832..6851
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7085..7102
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7342..7363
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7378..7383,7384..7401,7402..7408)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7416..7432
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7421..7443
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7440..7456
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7440..7456
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7506..7525
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7717..8350
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8397..8522
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9019..9252
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9437..9481
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9778..9895
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        join(9947..10235,1..15)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
       61 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      121 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      181 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
      241 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggcg gccgcagatc
      301 tctcgaggtt gatctaatac gactcactat agggagaccc aagctggcta gttaagctat
      361 caacaagttt gtacaaaaaa gcaggctccg cggccgcccc cttcaccatg gacggtttcg
      421 ccggcagtct cgatgatagt atttctgctg caagtacttc tgatgttcaa gatcgcctgt
      481 cagctcttga gtcacgagtt cagcaacaag aagatgaaat cactgtgcta aaggcggctt
      541 tggctgatgt tttgaggcgt cttgcaatct ctgaagatca tgtggcctca gtgaaaaaat
      601 cagtctcaag taaaggccaa ccaagccctc gagcagttat tcccatgtcc tgtataacca
      661 atggaagtgg tgcaaacaga aaaccaagtc ataccagtgc tgtctcaatt gcaggaaaag
      721 aaactctttc atctgctgct aaaagtggta cagaaaaaaa gaaagaaaaa ccacaaggac
      781 agagagaaaa aaaagaggaa tctcattcta atgatcaaag tccacaaatt cgagcatcac
      841 cttctcccca gccctcttca caacctctcc aaatacacag acaaactcca gaaagcaaga
      901 atgctactcc caccaaaagc ataaaacgac catcaccagc tgaaaagtca cataattctt
      961 gggaaaattc agatgatagc cgtaataaat tgtcgaaaat accttcaaca cccaaattaa
     1021 taccaaaagt taccaaaact gcagacaagc ataaagatgt catcatcaac caagtgtacc
     1081 gccggaagca ccaggagctg caagccatgc agatggagct gcagagccct gagtacaagc
     1141 tgagcaagct ccgcacctcg accatcatga ccgactacaa ccccaactac tgctttgctg
     1201 gcaagacctc ctccatcagt gacctgaagg aggtgccgcg gaaaaacatc accctcattc
     1261 ggggtctggg ccatggcgcc tttggggagg tgtatgaagg ccaggtgtcc ggaatgccca
     1321 acgacccaag ccccctgcaa gtggctgtga agacgctgcc tgaagtgtgc tctgaacagg
     1381 acgaactgga tttcctcatg gaagccctga tcaacagcaa attcaaccac cagaacattg
     1441 ttcgctgcat tggggtgagc ctgcaatccc tgccccggtt catcctgctg gagctcatgg
     1501 cggggggaaa cctcaagtcc ttcctccgag agacccgccc tcgcccgagc cagccctcct
     1561 ccctggccat gctggacctt ctgcacgtgg ctcgggacat tgcctgtggc tgtcagtatt
     1621 tggaggaaaa ccacttcatc caccgagaca ttgctgccag aaactgcctc ttgacctgtc
     1681 caggccctgg aagagtggcc aagattggag acttcgggat ggcccgagac atctacaggg
     1741 cgagctacta tagaaaggga ggctgtgcca tgctgccagt taagtggatg cccccagagg
     1801 ccttcatgga aggaatattc acttctaaaa cagacacatg gtcctttgga gtgctgctat
     1861 gggaaatctt ttctcttgga tatatgccat accccagcaa aagcaaccag gaagttctgg
     1921 agtttgtcac cagtggaggc cggatggacc cacccaagaa ctgccctggg cctgtatacc
     1981 ggataatgac tcagtgctgg caacatcagc ctgaagacag gcccaacttt gccatcattt
     2041 tggagaggat tgaatactgc acccaggacc cggatgtaat caacaccgct ttgccgatag
     2101 aatatggtcc acttgtggaa gaggaagaga aagtgcctgt gaggcccaag gaccctgagg
     2161 gggttcctcc tctcctggtc tctcaacagg caaaacggga ggaggagcgc agcccagctg
     2221 ccccaccacc tctgcctacc acctcctctg gcaaggctgc aaagaaaccc acagctgcag
     2281 aggtctctgt tcgagtccct agagggccgg ccgtggaagg gggacacgtg aatatggcat
     2341 tctctcagtc caaccctcct tcggagttgc acaaggtcca cggatccaga aacaagccca
     2401 ccagcttgtg gaacccaacg tacggctcct ggtttacaga gaaacccacc aaaaagaata
     2461 atcctatagc aaagaaggag ccacacgaca ggggtaacct ggggctggag ggaagctgta
     2521 ctgtcccacc taacgttgca actgggagac ttccgggggc ctcactgctc ctagagccct
     2581 cttcgctgac tgccaatatg aaggaggtac ctctgttcag gctacgtcac ttcccttgtg
     2641 ggaatgtcaa ttacggctac cagcaacagg gcttgccctt agaagccgct actgcccctg
     2701 gagctggtca ttacgaggat accattctga aaagcaagaa tagcatgaac cagcctgggc
     2761 cctgaaaggg tgggcgcgcc gacccagctt tcttgtacaa agtggtttag ctagagggcc
     2821 cgcggttcga actcgaggac tacaaggacg acgatgacaa gtcggccgct ggaggatacc
     2881 cctacgacgt gcccgactac gcctagaatt ccgccccccc ccctaacgtt actggccgaa
     2941 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     3001 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     3061 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     3121 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     3181 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     3241 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     3301 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     3361 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     3421 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
     3481 gccacaacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     3541 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     3601 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     3661 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     3721 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     3781 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     3841 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     3901 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     3961 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     4021 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     4081 ggtgcctgat gcatcgatgg atcctaatca acctctggat tacaaaattt gtgaaagatt
     4141 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4201 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4261 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4321 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4381 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4441 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4501 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4561 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4621 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     4681 ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt acaaggcagc tgtagatctt
     4741 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     4801 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     4861 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     4921 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     4981 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     5041 cttgcaaaga aatgaatatc agagagtgag aggcccgggt taattaagga aagggctaga
     5101 tcattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     5161 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5221 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5281 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5341 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5401 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5461 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5521 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     5581 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5641 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5701 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5761 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5821 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     5881 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5941 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     6001 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6061 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6121 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6181 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6241 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6301 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6361 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6421 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6481 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6541 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6601 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6661 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6721 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6781 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6841 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6901 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6961 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7021 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7081 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     7141 cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct gactaatttt ttttatttat
     7201 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7261 ggaggcctag gcttttgcaa aaagctcccc gtggcacgac aggtttcccg actggaaagc
     7321 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     7381 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     7441 aggaaacagc tatgacatga ttacgaattt cacaaataaa gcattttttt cactgcattc
     7501 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
     7561 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
     7621 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
     7681 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
     7741 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     7801 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     7861 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     7921 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     7981 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     8041 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     8101 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     8161 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8221 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8281 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8341 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     8401 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     8461 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     8521 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     8581 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     8641 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     8701 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     8761 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     8821 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
     8881 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     8941 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     9001 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     9061 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     9121 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     9181 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     9241 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     9301 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     9361 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     9421 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     9481 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     9541 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     9601 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     9661 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     9721 gaacggatct cgacggtatc gccgaattaa ttcacaaatg gcagtattca tccacaattt
     9781 taaaagaaaa ggggggattg gggggtacag tgcagggaaa agaatagtag acataatagc
     9841 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     9901 ttattacagg gacagcagag atccagtttg gactagtgga gttccgcgtt acataactta
     9961 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
    10021 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
    10081 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
    10141 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
    10201 actttcctac ttggcagtac atctacgtat tagtc
//
