LOCUS       sequence-360488-       10235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK 
            L1196M/G1202R mutation in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Yoda S, Lin JJ, Lawrence MS, Burke BJ, Friboulet L, Langenbucher A, 
            Dardaei L, Prutisto-Chang K, Dagogo-Jack I, Timofeevski S, Hubbeling
            H, Gainor JF, Ferris LA, Riley AK, Kattermann KE, Timonina D, Heist 
            RS, Iafrate AJ, Benes CH, Lennerz JK, Mino-Kenudson M, Engelman JA, 
            Johnson TW, Hata AN, Shaw AT
  TITLE     Sequential ALK Inhibitors Can Select for Lorlatinib-Resistant 
            Compound ALK Mutations in ALK-Positive Lung Cancer.
  JOURNAL   Cancer Discov. 2018 Jun;8(6):714-729. doi: 
            10.1158/2159-8290.CD-17-1256. Epub 2018 Apr 12.
  PUBMED    29650534
REFERENCE   2  (bases 1 to 10235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-17-1256"; journalName: "Cancer Discov"; date: 
            "2018-06"; volume: "8"; issue: "6"; pages: "714-729"
COMMENT     Sequence Label: pLenti-EML4-ALK variant 3a L1196M/G1202R
FEATURES             Location/Qualifiers
     source          1..10235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        152..355
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     306..326
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     352..376
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     451..470
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        451..469
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    499..523
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2918..2942)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2974..2997
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3013..3039
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3013..3039
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    3046..3612
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3062..3614
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3206..3223)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3433..3452
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3626..4225
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3626..3645)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4122..4142
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4242..4830
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4295..4315)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4713..4724)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4905..5138
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5257..5275)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5343..5447
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5448..5516,5517..6308)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5666..5685)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6479..7067
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6968..6987
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7221..7238
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7478..7499
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7514..7519,7520..7537,7538..7544)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7552..7568
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7557..7579
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7576..7592
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7576..7592
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7642..7661
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7853..8486
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8533..8658
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9155..9388
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9573..9617
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9914..10031
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        join(10083..10235,1..151)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
       61 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      121 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      181 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      241 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      301 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
      361 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
      421 ccggcggccg cagatctctc gaggttgatc taatacgact cactataggg agacccaagc
      481 tggctagtta agctatcaac aagtttgtac aaaaaagcag gctccgcggc cgcccccttc
      541 accatggacg gtttcgccgg cagtctcgat gatagtattt ctgctgcaag tacttctgat
      601 gttcaagatc gcctgtcagc tcttgagtca cgagttcagc aacaagaaga tgaaatcact
      661 gtgctaaagg cggctttggc tgatgttttg aggcgtcttg caatctctga agatcatgtg
      721 gcctcagtga aaaaatcagt ctcaagtaaa ggccaaccaa gccctcgagc agttattccc
      781 atgtcctgta taaccaatgg aagtggtgca aacagaaaac caagtcatac cagtgctgtc
      841 tcaattgcag gaaaagaaac tctttcatct gctgctaaaa gtggtacaga aaaaaagaaa
      901 gaaaaaccac aaggacagag agaaaaaaaa gaggaatctc attctaatga tcaaagtcca
      961 caaattcgag catcaccttc tccccagccc tcttcacaac ctctccaaat acacagacaa
     1021 actccagaaa gcaagaatgc tactcccacc aaaagcataa aacgaccatc accagctgaa
     1081 aagtcacata attcttggga aaattcagat gatagccgta ataaattgtc gaaaatacct
     1141 tcaacaccca aattaatacc aaaagttacc aaaactgcag acaagcataa agatgtcatc
     1201 atcaaccaag tgtaccgccg gaagcaccag gagctgcaag ccatgcagat ggagctgcag
     1261 agccctgagt acaagctgag caagctccgc acctcgacca tcatgaccga ctacaacccc
     1321 aactactgct ttgctggcaa gacctcctcc atcagtgacc tgaaggaggt gccgcggaaa
     1381 aacatcaccc tcattcgggg tctgggccat ggcgcctttg gggaggtgta tgaaggccag
     1441 gtgtccggaa tgcccaacga cccaagcccc ctgcaagtgg ctgtgaagac gctgcctgaa
     1501 gtgtgctctg aacaggacga actggatttc ctcatggaag ccctgatcat cagcaaattc
     1561 aaccaccaga acattgttcg ctgcattggg gtgagcctgc aatccctgcc ccggttcatc
     1621 ctgatggagc tcatggcggg gagagacctc aagtccttcc tccgagagac ccgccctcgc
     1681 ccgagccagc cctcctccct ggccatgctg gaccttctgc acgtggctcg ggacattgcc
     1741 tgtggctgtc agtatttgga ggaaaaccac ttcatccacc gagacattgc tgccagaaac
     1801 tgcctcttga cctgtccagg ccctggaaga gtggccaaga ttggagactt cgggatggcc
     1861 cgagacatct acagggcgag ctactataga aagggaggct gtgccatgct gccagttaag
     1921 tggatgcccc cagaggcctt catggaagga atattcactt ctaaaacaga cacatggtcc
     1981 tttggagtgc tgctatggga aatcttttct cttggatata tgccataccc cagcaaaagc
     2041 aaccaggaag ttctggagtt tgtcaccagt ggaggccgga tggacccacc caagaactgc
     2101 cctgggcctg tataccggat aatgactcag tgctggcaac atcagcctga agacaggccc
     2161 aactttgcca tcattttgga gaggattgaa tactgcaccc aggacccgga tgtaatcaac
     2221 accgctttgc cgatagaata tggtccactt gtggaagagg aagagaaagt gcctgtgagg
     2281 cccaaggacc ctgagggggt tcctcctctc ctggtctctc aacaggcaaa acgggaggag
     2341 gagcgcagcc cagctgcccc accacctctg cctaccacct cctctggcaa ggctgcaaag
     2401 aaacccacag ctgcagaggt ctctgttcga gtccctagag ggccggccgt ggaaggggga
     2461 cacgtgaata tggcattctc tcagtccaac cctccttcgg agttgcacaa ggtccacgga
     2521 tccagaaaca agcccaccag cttgtggaac ccaacgtacg gctcctggtt tacagagaaa
     2581 cccaccaaaa agaataatcc tatagcaaag aaggagccac acgacagggg taacctgggg
     2641 ctggagggaa gctgtactgt cccacctaac gttgcaactg ggagacttcc gggggcctca
     2701 ctgctcctag agccctcttc gctgactgcc aatatgaagg aggtacctct gttcaggcta
     2761 cgtcacttcc cttgtgggaa tgtcaattac ggctaccagc aacagggctt gcccttagaa
     2821 gccgctactg cccctggagc tggtcattac gaggatacca ttctgaaaag caagaatagc
     2881 atgaaccagc ctgggccctg aaagggtggg cgcgccgacc cagctttctt gtacaaagtg
     2941 gtttagctag agggcccgcg gttcgaactc gaggactaca aggacgacga tgacaagtcg
     3001 gccgctggag gataccccta cgacgtgccc gactacgcct agaattccgc ccccccccct
     3061 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     3121 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     3181 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     3241 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     3301 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     3361 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     3421 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     3481 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     3541 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     3601 acacgatgat aatatggcca caaccatgac cgagtacaag cccacggtgc gcctcgccac
     3661 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     3721 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     3781 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     3841 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     3901 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     3961 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     4021 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     4081 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     4141 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     4201 catgacccgc aagcccggtg cctgatgcat cgatggatcc taatcaacct ctggattaca
     4261 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4321 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4381 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4441 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4501 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4561 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4621 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     4681 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     4741 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     4801 gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa tgacttacaa
     4861 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     4921 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     4981 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5041 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5101 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     5161 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc ccgggttaat
     5221 taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     5281 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     5341 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5401 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5461 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5521 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5581 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5641 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     5701 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5761 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5821 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5881 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5941 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6001 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6061 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6121 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6181 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6241 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6301 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6361 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6421 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6481 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6541 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6601 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6661 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6721 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6781 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6841 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6901 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6961 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7021 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7081 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7141 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7201 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7261 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact
     7321 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7381 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt
     7441 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     7501 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     7561 gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca aataaagcat
     7621 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7681 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
     7741 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
     7801 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
     7861 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     7921 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
     7981 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
     8041 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     8101 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
     8161 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
     8221 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
     8281 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     8341 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     8401 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     8461 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     8521 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     8581 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     8641 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     8701 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     8761 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     8821 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     8881 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     8941 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     9001 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     9061 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     9121 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     9181 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     9241 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     9301 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     9361 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     9421 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     9481 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     9541 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     9601 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     9661 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     9721 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     9781 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     9841 gatccattcg attagtgaac ggatctcgac ggtatcgccg aattaattca caaatggcag
     9901 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca gggaaaagaa
     9961 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
    10021 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggact agtggagttc
    10081 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
    10141 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
    10201 caatgggtgg agtatttacg gtaaactgcc cactt
//
