LOCUS       sequence-360489-       10235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK G1202R 
            mutation in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Yoda S, Lin JJ, Lawrence MS, Burke BJ, Friboulet L, Langenbucher A, 
            Dardaei L, Prutisto-Chang K, Dagogo-Jack I, Timofeevski S, Hubbeling
            H, Gainor JF, Ferris LA, Riley AK, Kattermann KE, Timonina D, Heist 
            RS, Iafrate AJ, Benes CH, Lennerz JK, Mino-Kenudson M, Engelman JA, 
            Johnson TW, Hata AN, Shaw AT
  TITLE     Sequential ALK Inhibitors Can Select for Lorlatinib-Resistant 
            Compound ALK Mutations in ALK-Positive Lung Cancer.
  JOURNAL   Cancer Discov. 2018 Jun;8(6):714-729. doi: 
            10.1158/2159-8290.CD-17-1256. Epub 2018 Apr 12.
  PUBMED    29650534
REFERENCE   2  (bases 1 to 10235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-17-1256"; journalName: "Cancer Discov"; date: 
            "2018-06"; volume: "8"; issue: "6"; pages: "714-729"
COMMENT     Sequence Label: pLenti-EML4-ALK variant 3a G1202R
FEATURES             Location/Qualifiers
     source          1..10235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     142..162
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     188..212
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     287..306
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        287..305
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    335..359
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2754..2778)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2810..2833
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2849..2875
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2849..2875
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2882..3448
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2898..3450
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3042..3059)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3269..3288
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3462..4061
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3462..3481)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3958..3978
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4078..4666
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4131..4151)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4549..4560)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4741..4974
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5093..5111)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5179..5283
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5284..5352,5353..6144)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5502..5521)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6315..6903
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6804..6823
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7057..7074
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7314..7335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7350..7355,7356..7373,7374..7380)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7388..7404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7393..7415
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7412..7428
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7412..7428
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7478..7497
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7689..8322
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8369..8494
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8991..9224
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9409..9453
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9750..9867
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        9919..10222
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(10223..10235,1..191)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
       61 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      121 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      181 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
      241 acctccatag aagacaccgg cggccgcaga tctctcgagg ttgatctaat acgactcact
      301 atagggagac ccaagctggc tagttaagct atcaacaagt ttgtacaaaa aagcaggctc
      361 cgcggccgcc cccttcacca tggacggttt cgccggcagt ctcgatgata gtatttctgc
      421 tgcaagtact tctgatgttc aagatcgcct gtcagctctt gagtcacgag ttcagcaaca
      481 agaagatgaa atcactgtgc taaaggcggc tttggctgat gttttgaggc gtcttgcaat
      541 ctctgaagat catgtggcct cagtgaaaaa atcagtctca agtaaaggcc aaccaagccc
      601 tcgagcagtt attcccatgt cctgtataac caatggaagt ggtgcaaaca gaaaaccaag
      661 tcataccagt gctgtctcaa ttgcaggaaa agaaactctt tcatctgctg ctaaaagtgg
      721 tacagaaaaa aagaaagaaa aaccacaagg acagagagaa aaaaaagagg aatctcattc
      781 taatgatcaa agtccacaaa ttcgagcatc accttctccc cagccctctt cacaacctct
      841 ccaaatacac agacaaactc cagaaagcaa gaatgctact cccaccaaaa gcataaaacg
      901 accatcacca gctgaaaagt cacataattc ttgggaaaat tcagatgata gccgtaataa
      961 attgtcgaaa ataccttcaa cacccaaatt aataccaaaa gttaccaaaa ctgcagacaa
     1021 gcataaagat gtcatcatca accaagtgta ccgccggaag caccaggagc tgcaagccat
     1081 gcagatggag ctgcagagcc ctgagtacaa gctgagcaag ctccgcacct cgaccatcat
     1141 gaccgactac aaccccaact actgctttgc tggcaagacc tcctccatca gtgacctgaa
     1201 ggaggtgccg cggaaaaaca tcaccctcat tcggggtctg ggccatggcg cctttgggga
     1261 ggtgtatgaa ggccaggtgt ccggaatgcc caacgaccca agccccctgc aagtggctgt
     1321 gaagacgctg cctgaagtgt gctctgaaca ggacgaactg gatttcctca tggaagccct
     1381 gatcatcagc aaattcaacc accagaacat tgttcgctgc attggggtga gcctgcaatc
     1441 cctgccccgg ttcatcctgc tggagctcat ggcggggaga gacctcaagt ccttcctccg
     1501 agagacccgc cctcgcccga gccagccctc ctccctggcc atgctggacc ttctgcacgt
     1561 ggctcgggac attgcctgtg gctgtcagta tttggaggaa aaccacttca tccaccgaga
     1621 cattgctgcc agaaactgcc tcttgacctg tccaggccct ggaagagtgg ccaagattgg
     1681 agacttcggg atggcccgag acatctacag ggcgagctac tatagaaagg gaggctgtgc
     1741 catgctgcca gttaagtgga tgcccccaga ggccttcatg gaaggaatat tcacttctaa
     1801 aacagacaca tggtcctttg gagtgctgct atgggaaatc ttttctcttg gatatatgcc
     1861 ataccccagc aaaagcaacc aggaagttct ggagtttgtc accagtggag gccggatgga
     1921 cccacccaag aactgccctg ggcctgtata ccggataatg actcagtgct ggcaacatca
     1981 gcctgaagac aggcccaact ttgccatcat tttggagagg attgaatact gcacccagga
     2041 cccggatgta atcaacaccg ctttgccgat agaatatggt ccacttgtgg aagaggaaga
     2101 gaaagtgcct gtgaggccca aggaccctga gggggttcct cctctcctgg tctctcaaca
     2161 ggcaaaacgg gaggaggagc gcagcccagc tgccccacca cctctgccta ccacctcctc
     2221 tggcaaggct gcaaagaaac ccacagctgc agaggtctct gttcgagtcc ctagagggcc
     2281 ggccgtggaa gggggacacg tgaatatggc attctctcag tccaaccctc cttcggagtt
     2341 gcacaaggtc cacggatcca gaaacaagcc caccagcttg tggaacccaa cgtacggctc
     2401 ctggtttaca gagaaaccca ccaaaaagaa taatcctata gcaaagaagg agccacacga
     2461 caggggtaac ctggggctgg agggaagctg tactgtccca cctaacgttg caactgggag
     2521 acttccgggg gcctcactgc tcctagagcc ctcttcgctg actgccaata tgaaggaggt
     2581 acctctgttc aggctacgtc acttcccttg tgggaatgtc aattacggct accagcaaca
     2641 gggcttgccc ttagaagccg ctactgcccc tggagctggt cattacgagg ataccattct
     2701 gaaaagcaag aatagcatga accagcctgg gccctgaaag ggtgggcgcg ccgacccagc
     2761 tttcttgtac aaagtggttt agctagaggg cccgcggttc gaactcgagg actacaagga
     2821 cgacgatgac aagtcggccg ctggaggata cccctacgac gtgcccgact acgcctagaa
     2881 ttccgccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     2941 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     3001 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     3061 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     3121 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     3181 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     3241 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     3301 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     3361 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     3421 ggttttcctt tgaaaaacac gatgataata tggccacaac catgaccgag tacaagccca
     3481 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     3541 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     3601 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     3661 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     3721 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     3781 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     3841 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     3901 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     3961 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     4021 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg atgcatcgat ggatcctaat
     4081 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     4141 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     4201 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     4261 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     4321 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     4381 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4441 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     4501 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4561 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4621 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga gatcctttaa
     4681 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     4741 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     4801 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4861 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     4921 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     4981 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     5041 agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg
     5101 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     5161 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5221 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5281 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5341 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5401 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5461 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5521 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5581 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5641 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5701 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5761 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5821 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5881 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5941 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6001 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6061 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6121 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6181 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6241 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6301 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6361 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6421 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6481 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6541 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6601 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6661 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6721 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6781 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6841 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6901 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6961 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     7021 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7081 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     7141 aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     7201 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     7261 ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     7321 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     7381 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat
     7441 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7501 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
     7561 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
     7621 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
     7681 ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat
     7741 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     7801 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     7861 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     7921 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     7981 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     8041 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     8101 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     8161 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8221 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8281 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     8341 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     8401 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     8461 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     8521 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     8581 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     8641 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     8701 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     8761 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     8821 cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat
     8881 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     8941 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     9001 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     9061 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     9121 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     9181 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     9241 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     9301 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     9361 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     9421 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     9481 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     9541 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     9601 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     9661 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccgaatt
     9721 aattcacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     9781 agtgcaggga aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     9841 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     9901 tggactagtg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     9961 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
    10021 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
    10081 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
    10141 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
    10201 attagtcatc gctattacca tggtgatgcg gtttt
//
