LOCUS       sequence-360496-       10235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express EML4-ALK fusion variant 3a (E6a;A20) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Yoda S, Lin JJ, Lawrence MS, Burke BJ, Friboulet L, Langenbucher A, 
            Dardaei L, Prutisto-Chang K, Dagogo-Jack I, Timofeevski S, Hubbeling
            H, Gainor JF, Ferris LA, Riley AK, Kattermann KE, Timonina D, Heist 
            RS, Iafrate AJ, Benes CH, Lennerz JK, Mino-Kenudson M, Engelman JA, 
            Johnson TW, Hata AN, Shaw AT
  TITLE     Sequential ALK Inhibitors Can Select for Lorlatinib-Resistant 
            Compound ALK Mutations in ALK-Positive Lung Cancer.
  JOURNAL   Cancer Discov. 2018 Jun;8(6):714-729. doi: 
            10.1158/2159-8290.CD-17-1256. Epub 2018 Apr 12.
  PUBMED    29650534
REFERENCE   2  (bases 1 to 10235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-17-1256"; journalName: "Cancer Discov"; date: 
            "2018-06"; volume: "8"; issue: "6"; pages: "714-729"
COMMENT     Sequence Label: pLenti-EML4-ALK variant 3a
FEATURES             Location/Qualifiers
     source          1..10235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     83..103
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     129..153
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     228..247
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        228..246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    276..300
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2695..2719)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2751..2774
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2790..2816
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2790..2816
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2823..3389
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2839..3391
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2983..3000)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3210..3229
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3403..4002
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3403..3422)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3899..3919
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4019..4607
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4072..4092)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4490..4501)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4682..4915
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5034..5052)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5120..5224
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5225..5293,5294..6085)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5443..5462)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6256..6844
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6745..6764
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6998..7015
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7255..7276
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7291..7296,7297..7314,7315..7321)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7329..7345
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7334..7356
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7353..7369
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7353..7369
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7419..7438
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7630..8263
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8310..8435
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8932..9165
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9350..9394
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9691..9808
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        9860..10163
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(10164..10235,1..132)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
       61 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      121 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      181 gacctccata gaagacaccg gcggccgcag atctctcgag gttgatctaa tacgactcac
      241 tatagggaga cccaagctgg ctagttaagc tatcaacaag tttgtacaaa aaagcaggct
      301 ccgcggccgc ccccttcacc atggacggtt tcgccggcag tctcgatgat agtatttctg
      361 ctgcaagtac ttctgatgtt caagatcgcc tgtcagctct tgagtcacga gttcagcaac
      421 aagaagatga aatcactgtg ctaaaggcgg ctttggctga tgttttgagg cgtcttgcaa
      481 tctctgaaga tcatgtggcc tcagtgaaaa aatcagtctc aagtaaaggc caaccaagcc
      541 ctcgagcagt tattcccatg tcctgtataa ccaatggaag tggtgcaaac agaaaaccaa
      601 gtcataccag tgctgtctca attgcaggaa aagaaactct ttcatctgct gctaaaagtg
      661 gtacagaaaa aaagaaagaa aaaccacaag gacagagaga aaaaaaagag gaatctcatt
      721 ctaatgatca aagtccacaa attcgagcat caccttctcc ccagccctct tcacaacctc
      781 tccaaataca cagacaaact ccagaaagca agaatgctac tcccaccaaa agcataaaac
      841 gaccatcacc agctgaaaag tcacataatt cttgggaaaa ttcagatgat agccgtaata
      901 aattgtcgaa aataccttca acacccaaat taataccaaa agttaccaaa actgcagaca
      961 agcataaaga tgtcatcatc aaccaagtgt accgccggaa gcaccaggag ctgcaagcca
     1021 tgcagatgga gctgcagagc cctgagtaca agctgagcaa gctccgcacc tcgaccatca
     1081 tgaccgacta caaccccaac tactgctttg ctggcaagac ctcctccatc agtgacctga
     1141 aggaggtgcc gcggaaaaac atcaccctca ttcggggtct gggccatggc gcctttgggg
     1201 aggtgtatga aggccaggtg tccggaatgc ccaacgaccc aagccccctg caagtggctg
     1261 tgaagacgct gcctgaagtg tgctctgaac aggacgaact ggatttcctc atggaagccc
     1321 tgatcatcag caaattcaac caccagaaca ttgttcgctg cattggggtg agcctgcaat
     1381 ccctgccccg gttcatcctg ctggagctca tggcgggggg agacctcaag tccttcctcc
     1441 gagagacccg ccctcgcccg agccagccct cctccctggc catgctggac cttctgcacg
     1501 tggctcggga cattgcctgt ggctgtcagt atttggagga aaaccacttc atccaccgag
     1561 acattgctgc cagaaactgc ctcttgacct gtccaggccc tggaagagtg gccaagattg
     1621 gagacttcgg gatggcccga gacatctaca gggcgagcta ctatagaaag ggaggctgtg
     1681 ccatgctgcc agttaagtgg atgcccccag aggccttcat ggaaggaata ttcacttcta
     1741 aaacagacac atggtccttt ggagtgctgc tatgggaaat cttttctctt ggatatatgc
     1801 cataccccag caaaagcaac caggaagttc tggagtttgt caccagtgga ggccggatgg
     1861 acccacccaa gaactgccct gggcctgtat accggataat gactcagtgc tggcaacatc
     1921 agcctgaaga caggcccaac tttgccatca ttttggagag gattgaatac tgcacccagg
     1981 acccggatgt aatcaacacc gctttgccga tagaatatgg tccacttgtg gaagaggaag
     2041 agaaagtgcc tgtgaggccc aaggaccctg agggggttcc tcctctcctg gtctctcaac
     2101 aggcaaaacg ggaggaggag cgcagcccag ctgccccacc acctctgcct accacctcct
     2161 ctggcaaggc tgcaaagaaa cccacagctg cagaggtctc tgttcgagtc cctagagggc
     2221 cggccgtgga agggggacac gtgaatatgg cattctctca gtccaaccct ccttcggagt
     2281 tgcacaaggt ccacggatcc agaaacaagc ccaccagctt gtggaaccca acgtacggct
     2341 cctggtttac agagaaaccc accaaaaaga ataatcctat agcaaagaag gagccacacg
     2401 acaggggtaa cctggggctg gagggaagct gtactgtccc acctaacgtt gcaactggga
     2461 gacttccggg ggcctcactg ctcctagagc cctcttcgct gactgccaat atgaaggagg
     2521 tacctctgtt caggctacgt cacttccctt gtgggaatgt caattacggc taccagcaac
     2581 agggcttgcc cttagaagcc gctactgccc ctggagctgg tcattacgag gataccattc
     2641 tgaaaagcaa gaatagcatg aaccagcctg ggccctgaaa gggtgggcgc gccgacccag
     2701 ctttcttgta caaagtggtt tagctagagg gcccgcggtt cgaactcgag gactacaagg
     2761 acgacgatga caagtcggcc gctggaggat acccctacga cgtgcccgac tacgcctaga
     2821 attccgcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     2881 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     2941 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     3001 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     3061 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     3121 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     3181 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     3241 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     3301 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     3361 tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatgaccga gtacaagccc
     3421 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     3481 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     3541 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     3601 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     3661 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     3721 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     3781 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     3841 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     3901 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     3961 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gatgcatcga tggatcctaa
     4021 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     4081 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     4141 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     4201 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     4261 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     4321 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     4381 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     4441 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     4501 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4561 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta
     4621 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     4681 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     4741 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     4801 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     4861 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     4921 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     4981 gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
     5041 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     5101 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5161 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5221 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5281 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5341 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5401 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5461 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5521 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5581 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5641 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5701 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5761 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5821 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5881 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5941 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6001 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6061 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6121 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6181 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6241 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6301 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6361 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6421 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6481 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6541 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6601 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6661 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6721 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6781 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6841 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6901 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6961 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7021 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     7081 caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7141 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     7201 cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     7261 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     7321 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     7381 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7441 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     7501 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     7561 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     7621 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     7681 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     7741 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     7801 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     7861 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     7921 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     7981 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     8041 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     8101 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     8161 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     8221 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     8281 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     8341 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     8401 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     8461 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     8521 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     8581 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     8641 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     8701 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     8761 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     8821 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     8881 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     8941 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     9001 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     9061 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     9121 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     9181 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     9241 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     9301 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     9361 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     9421 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     9481 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     9541 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     9601 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat
     9661 taattcacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     9721 cagtgcaggg aaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     9781 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     9841 ttggactagt ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     9901 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     9961 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
    10021 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
    10081 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
    10141 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
    10201 agcggtttga ctcacgggga tttccaagtc tccac
//
