LOCUS       sequence-360502-       11057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express EML4-ALK fusion variant 1 (E13;A20) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Yoda S, Lin JJ, Lawrence MS, Burke BJ, Friboulet L, Langenbucher A, 
            Dardaei L, Prutisto-Chang K, Dagogo-Jack I, Timofeevski S, Hubbeling
            H, Gainor JF, Ferris LA, Riley AK, Kattermann KE, Timonina D, Heist 
            RS, Iafrate AJ, Benes CH, Lennerz JK, Mino-Kenudson M, Engelman JA, 
            Johnson TW, Hata AN, Shaw AT
  TITLE     Sequential ALK Inhibitors Can Select for Lorlatinib-Resistant 
            Compound ALK Mutations in ALK-Positive Lung Cancer.
  JOURNAL   Cancer Discov. 2018 Jun;8(6):714-729. doi: 
            10.1158/2159-8290.CD-17-1256. Epub 2018 Apr 12.
  PUBMED    29650534
REFERENCE   2  (bases 1 to 11057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-17-1256"; journalName: "Cancer Discov"; date: 
            "2018-06"; volume: "8"; issue: "6"; pages: "714-729"
COMMENT     Sequence Label: pLenti-EML4-ALK variant 1
FEATURES             Location/Qualifiers
     source          1..11057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..211
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     162..182
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     208..232
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     307..326
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        307..325
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    355..379
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3596..3620)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3652..3675
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3691..3717
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3691..3717
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    3724..4290
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3740..4292
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3884..3901)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4111..4130
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4304..4903
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4304..4323)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4800..4820
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4920..5508
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4973..4993)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5391..5402)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5583..5816
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5935..5953)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6021..6125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6126..6194,6195..6986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6344..6363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7157..7745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7646..7665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7899..7916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8156..8177
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8192..8197,8198..8215,8216..8222)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8230..8246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8235..8257
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8254..8270
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8254..8270
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8320..8339
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8531..9164
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9211..9336
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9833..10066
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10251..10295
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10592..10709
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        join(10761..11057,1..7)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
       61 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      121 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      181 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
      241 acgccatcca cgctgttttg acctccatag aagacaccgg cggccgcaga tctctcgagg
      301 ttgatctaat acgactcact atagggagac ccaagctggc tagttaagct atcaacaagt
      361 ttgtacaaaa aagcaggctc cgcggccgcc cccttcacca tggacggttt cgccggcagt
      421 ctcgatgata gtatttctgc tgcaagtact tctgatgttc aagatcgcct gtcagctctt
      481 gagtcacgag ttcagcaaca agaagatgaa atcactgtgc taaaggcggc tttggctgat
      541 gttttgaggc gtcttgcaat ctctgaagat catgtggcct cagtgaaaaa atcagtctca
      601 agtaaaggcc aaccaagccc tcgagcagtt attcccatgt cctgtataac caatggaagt
      661 ggtgcaaaca gaaaaccaag tcataccagt gctgtctcaa ttgcaggaaa agaaactctt
      721 tcatctgctg ctaaaagtgg tacagaaaaa aagaaagaaa aaccacaagg acagagagaa
      781 aaaaaagagg aatctcattc taatgatcaa agtccacaaa ttcgagcatc accttctccc
      841 cagccctctt cacaacctct ccaaatacac agacaaactc cagaaagcaa gaatgctact
      901 cccaccaaaa gcataaaacg accatcacca gctgaaaagt cacataattc ttgggaaaat
      961 tcagatgata gccgtaataa attgtcgaaa ataccttcaa cacccaaatt aataccaaaa
     1021 gttaccaaaa ctgcagacaa gcataaagat gtcatcatca accaagaagg agaatatatt
     1081 aaaatgttta tgcgcggtcg gccaattacc atgttcattc cttccgatgt tgacaactat
     1141 gatgacatca gaacggaact gcctcctgag aagctcaaac tggagtgggc atatggttat
     1201 cgaggaaagg actgtagagc taatgtttac cttcttccga ccggggaaat agtttatttc
     1261 attgcatcag tagtagtact atttaattat gaggagagaa ctcagcgaca ctacctgggc
     1321 catacagact gtgtgaaatg ccttgctata catcctgaca aaattaggat tgcaactgga
     1381 cagatagctg gcgtggataa agatggaagg cctctacaac cccacgtcag agtgtgggat
     1441 tctgttactc tatccacact gcagattatt ggacttggca cttttgagcg tggagtagga
     1501 tgcctggatt tttcaaaagc agattcaggt gttcatttat gtgttattga tgactccaat
     1561 gagcatatgc ttactgtatg ggactggcag aagaaagcaa aaggagcaga aataaagaca
     1621 acaaatgaag ttgttttggc tgtggagttt cacccaacag atgcaaatac cataattaca
     1681 tgcggtaaat ctcatatttt cttctggacc tggagcggca attcactaac aagaaaacag
     1741 ggaatttttg ggaaatatga aaagccaaaa tttgtgcagt gtttagcatt cttggggaat
     1801 ggagatgttc ttactggaga ctcaggtgga gtcatgctta tatggagcaa aactactgta
     1861 gagcccacac ctgggaaagg acctaaagtg taccgccgga agcaccagga gctgcaagcc
     1921 atgcagatgg agctgcagag ccctgagtac aagctgagca agctccgcac ctcgaccatc
     1981 atgaccgact acaaccccaa ctactgcttt gctggcaaga cctcctccat cagtgacctg
     2041 aaggaggtgc cgcggaaaaa catcaccctc attcggggtc tgggccatgg cgcctttggg
     2101 gaggtgtatg aaggccaggt gtccggaatg cccaacgacc caagccccct gcaagtggct
     2161 gtgaagacgc tgcctgaagt gtgctctgaa caggacgaac tggatttcct catggaagcc
     2221 ctgatcatca gcaaattcaa ccaccagaac attgttcgct gcattggggt gagcctgcaa
     2281 tccctgcccc ggttcatcct gctggagctc atggcggggg gagacctcaa gtccttcctc
     2341 cgagagaccc gccctcgccc gagccagccc tcctccctgg ccatgctgga ccttctgcac
     2401 gtggctcggg acattgcctg tggctgtcag tatttggagg aaaaccactt catccaccga
     2461 gacattgctg ccagaaactg cctcttgacc tgtccaggcc ctggaagagt ggccaagatt
     2521 ggagacttcg ggatggcccg agacatctac agggcgagct actatagaaa gggaggctgt
     2581 gccatgctgc cagttaagtg gatgccccca gaggccttca tggaaggaat attcacttct
     2641 aaaacagaca catggtcctt tggagtgctg ctatgggaaa tcttttctct tggatatatg
     2701 ccatacccca gcaaaagcaa ccaggaagtt ctggagtttg tcaccagtgg aggccggatg
     2761 gacccaccca agaactgccc tgggcctgta taccggataa tgactcagtg ctggcaacat
     2821 cagcctgaag acaggcccaa ctttgccatc attttggaga ggattgaata ctgcacccag
     2881 gacccggatg taatcaacac cgctttgccg atagaatatg gtccacttgt ggaagaggaa
     2941 gagaaagtgc ctgtgaggcc caaggaccct gagggggttc ctcctctcct ggtctctcaa
     3001 caggcaaaac gggaggagga gcgcagccca gctgccccac cacctctgcc taccacctcc
     3061 tctggcaagg ctgcaaagaa acccacagct gcagaggtct ctgttcgagt ccctagaggg
     3121 ccggccgtgg aagggggaca cgtgaatatg gcattctctc agtccaaccc tccttcggag
     3181 ttgcacaagg tccacggatc cagaaacaag cccaccagct tgtggaaccc aacgtacggc
     3241 tcctggttta cagagaaacc caccaaaaag aataatccta tagcaaagaa ggagccacac
     3301 gacaggggta acctggggct ggagggaagc tgtactgtcc cacctaacgt tgcaactggg
     3361 agacttccgg gggcctcact gctcctagag ccctcttcgc tgactgccaa tatgaaggag
     3421 gtacctctgt tcaggctacg tcacttccct tgtgggaatg tcaattacgg ctaccagcaa
     3481 cagggcttgc ccttagaagc cgctactgcc cctggagctg gtcattacga ggataccatt
     3541 ctgaaaagca agaatagcat gaaccagcct gggccctgaa agggtgggcg cgccgaccca
     3601 gctttcttgt acaaagtggt ttagctagag ggcccgcggt tcgaactcga ggactacaag
     3661 gacgacgatg acaagtcggc cgctggagga tacccctacg acgtgcccga ctacgcctag
     3721 aattccgccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     3781 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     3841 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     3901 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     3961 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     4021 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     4081 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     4141 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     4201 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     4261 gtggttttcc tttgaaaaac acgatgataa tatggccaca accatgaccg agtacaagcc
     4321 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     4381 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     4441 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     4501 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     4561 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     4621 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     4681 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     4741 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     4801 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     4861 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgatgcatcg atggatccta
     4921 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     4981 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5041 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5101 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5161 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5221 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5281 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     5341 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5401 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5461 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gagatccttt
     5521 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5581 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     5641 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5701 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5761 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     5821 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     5881 tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga aagggcctcg
     5941 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     6001 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6061 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6361 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     7021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7981 gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8041 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     8101 ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     8161 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     8221 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga
     8281 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8341 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
     8401 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     8461 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     8521 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
     8581 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     8641 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     8701 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     8761 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     8821 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     8881 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     8941 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     9001 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     9061 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     9121 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     9181 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     9241 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     9301 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     9361 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     9421 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     9481 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     9541 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     9601 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     9661 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     9721 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     9781 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     9841 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     9901 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     9961 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
    10021 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
    10081 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
    10141 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
    10201 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
    10261 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
    10321 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
    10381 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
    10441 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
    10501 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa
    10561 ttaattcaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
    10621 acagtgcagg gaaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
    10681 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
    10741 tttggactag tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
    10801 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
    10861 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
    10921 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
    10981 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
    11041 gtattagtca tcgctat
//
