LOCUS       sequence-360503-        9989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CD74-ROS1 fusion (C6;R34) harboring ROS1 
            S1986F/G2032R/L2086F mutation in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Lin JJ, Choudhury NJ, Yoda S, Zhu VW, Johnson TW, Sakhtemani R, 
            Dagogo-Jack I, Digumarthy SR, Lee C, Do A, Peterson J, 
            Prutisto-Chang K, Malik W, Hubbeling HG, Langenbucher A, Schoenfeld 
            AJ, Falcon CJ, Temel JS, Sequist LV, Yeap BY, Lennerz JK, Shaw AT, 
            Lawrence MS, Ou SI, Hata AN, Drilon A, Gainor JF
  TITLE     Spectrum of Mechanisms of Resistance to Crizotinib and Lorlatinib in
            ROS1 Fusion-Positive Lung Cancer.
  JOURNAL   Clin Cancer Res. 2021 May 15;27(10):2899-2909. doi: 
            10.1158/1078-0432.CCR-21-0032. Epub 2021 Mar 8.
  PUBMED    33685866
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/1078-0432.CCR-21-0032"; journalName: "Clin Cancer Res"; 
            date: "2021-05-15- 15"; volume: "27"; issue: "10"; pages: 
            "2899-2909"
COMMENT     Sequence Label: pLenti-CD74-ROS1 S1986F/G2032R/L2086F
FEATURES             Location/Qualifiers
     source          1..9989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        11..214
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     165..185
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     211..235
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     310..329
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        310..328
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    358..382
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2531..2555)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2587..2610
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2626..2652
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2626..2652
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2659..3225
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2675..3227
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2819..2836)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3046..3065
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3239..3838
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3239..3258)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3735..3755
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3855..4443
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3908..3928)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4326..4337)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4518..4751
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4870..4888)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4956..5060
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5061..5129,5130..5921)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5279..5298)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6092..6680
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6581..6600
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6834..6851
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7091..7112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7127..7132,7133..7150,7151..7157)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7165..7181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7170..7192
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7189..7205
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7189..7205
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7255..7274
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7466..8099
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8146..8271
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8768..9001
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9186..9230
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9527..9644
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        join(9696..9989,1..10)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
       61 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      121 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      181 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
      241 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccgc agatctctcg
      301 aggttgatct aatacgactc actataggga gacccaagct ggctagttaa gctatcaaca
      361 agtttgtaca aaaaagcagg ctccgcggcc gcccccttca ccatgcacag gaggagaagc
      421 aggagctgtc gggaagatca gaagccagtc atggatgacc agcgcgacct tatctccaac
      481 aatgagcaac tgcccatgct gggccggcgc cctggggccc cggagagcaa gtgcagccgc
      541 ggagccctgt acacaggctt ttccatcctg gtgactctgc tcctcgctgg ccaggccacc
      601 accgcctact tcctgtacca gcagcagggc cggctggaca aactgacagt cacctcccag
      661 aacctgcagc tggagaacct gcgcatgaag cttcccaagc ctcccaagcc tgtgagcaag
      721 atgcgcatgg ccaccccgct gctgatgcag gcgctgccca tgggagccct gccccagggg
      781 cccatgcaga atgccaccaa gtatggcaac atgacagagg accatgtgat gcacctgctc
      841 cagaatgctg accccctgaa ggtgtacccg ccactgaagg ggagcttccc ggagaacctg
      901 agacacctta agaacaccat ggagaccata gactggaagg tctttgagag ctggatgcac
      961 cattggctcc tgtttgaaat gagcaggcac tccttggagc aaaagcccac tgacgctcca
     1021 ccgaaagatg atttttggat accagaaaca agtttcatac ttactattat agttggaata
     1081 tttctggttg ttacaatccc actgaccttt gtctggcata gaagattaaa gaatcaaaaa
     1141 agtgccaagg aaggggtgac agtgcttata aacgaagaca aagagttggc tgagctgcga
     1201 ggtctggcag ccggagtagg cctggctaat gcctgctatg caatacatac tcttccaacc
     1261 caagaggaga ttgaaaatct tcctgccttc cctcgggaaa aactgactct gcgtctcttg
     1321 ctgggaagtg gagcctttgg agaagtgtat gaaggaacag cagtggacat cttaggagtt
     1381 ggaagtggag aaatcaaagt agcagtgaag actttgaaga agggtttcac agaccaggag
     1441 aagattgaat tcctgaagga ggcacatctg atgagcaaat ttaatcatcc caacattctg
     1501 aagcagcttg gagtttgtct gctgaatgaa ccccaataca ttatcctgga actgatggag
     1561 ggaagagacc ttcttactta tttgcgtaaa gcccggatgg caacgtttta tggtccttta
     1621 ctcaccttgg ttgaccttgt agacctgtgt gtagatattt caaaaggctg tgtctacttg
     1681 gaacggatgc atttcattca cagggatctg gcagctagaa attgctttgt ttccgtgaaa
     1741 gactatacca gtccacggat agtgaagatt ggagactttg gactcgccag agacatctat
     1801 aaaaatgatt actatagaaa gagaggggaa ggcctgctcc cagttcggtg gatggctcca
     1861 gaaagtttga tggatggaat cttcactact caatctgatg tatggtcttt tggaattctg
     1921 atttgggaga ttttaactct tggtcatcag ccttatccag ctcattccaa ccttgatgtg
     1981 ttaaactatg tgcaaacagg agggagactg gagccaccaa gaaattgtcc tgatgatctg
     2041 tggaatttaa tgacccagtg ctgggctcaa gaacccgacc aaagacctac ttttcataga
     2101 attcaggacc aacttcagtt attcagaaat tttttcttaa atagcattta taagtccaga
     2161 gatgaagcaa acaacagtgg agtcataaat gaaagctttg aaggtgaaga tggcgatgtg
     2221 atttgtttga attcagatga cattatgcca gttgctttaa tggaaacgaa gaaccgagaa
     2281 gggttaaact atatggtact tgctacagaa tgtggccaag gtgaagaaaa gtctgagggt
     2341 cctctaggct cccaggaatc tgaatcttgt ggtctgagga aagaagagaa ggaaccacat
     2401 gcagacaaag atttctgcca agaaaaacaa gtggcttact gcccttctgg caagcctgaa
     2461 ggcctgaact atgcctgtct cactcacagt ggatatggag atgggtctga ttaaaagggt
     2521 gggcgcgccg acccagcttt cttgtacaaa gtggtttagc tagagggccc gcggttcgaa
     2581 ctcgaggact acaaggacga cgatgacaag tcggccgctg gaggataccc ctacgacgtg
     2641 cccgactacg cctagaattc cgcccccccc cctaacgtta ctggccgaag ccgcttggaa
     2701 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     2761 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     2821 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     2881 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     2941 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     3001 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     3061 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     3121 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     3181 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat
     3241 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     3301 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     3361 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     3421 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     3481 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     3541 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     3601 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     3661 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     3721 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     3781 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatg
     3841 catcgatgga tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     3901 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     3961 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4021 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     4081 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     4141 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4201 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     4261 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     4321 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     4381 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     4441 cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     4501 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     4561 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     4621 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4681 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4741 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     4801 atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat cattcttgaa
     4861 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     4921 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4981 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5041 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5101 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5161 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5221 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5281 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     5341 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5401 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5461 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5521 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5581 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5641 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5701 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5761 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5821 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5881 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5941 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6001 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6061 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6121 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6181 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6241 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6301 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6361 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6421 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6481 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6541 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6601 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6661 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6721 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6781 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6841 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     6901 cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga
     6961 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     7021 cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     7081 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     7141 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     7201 atgacatgat tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7261 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
     7321 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
     7381 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
     7441 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
     7501 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     7561 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     7621 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     7681 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     7741 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     7801 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     7861 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     7921 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7981 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8041 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8101 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     8161 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     8221 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     8281 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     8341 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     8401 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     8461 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     8521 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     8581 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
     8641 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     8701 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     8761 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     8821 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     8881 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     8941 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     9001 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     9061 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     9121 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     9181 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     9241 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     9301 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     9361 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     9421 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
     9481 gacggtatcg ccgaattaat tcacaaatgg cagtattcat ccacaatttt aaaagaaaag
     9541 gggggattgg ggggtacagt gcagggaaaa gaatagtaga cataatagca acagacatac
     9601 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     9661 acagcagaga tccagtttgg actagtggag ttccgcgtta cataacttac ggtaaatggc
     9721 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     9781 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     9841 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     9901 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     9961 tggcagtaca tctacgtatt agtcatcgc
//
