LOCUS       sequence-360504-       23762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  B6J 
            Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-H2B-sfGFP-T2A-tdTomato-WPRE-bpA 
            allele targeting vector, low copy number.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 23762)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     Miscellaneous gene targeting vector plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 23762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pB6-Hipp11-H2B-sfGFP-T2A-tdTomato-cre reporter...
FEATURES             Location/Qualifiers
     source          1..23762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        164..663
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             690..1262
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          1339..1404
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    1478..1702
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1764..1784)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1764..1782)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1819..1838)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1863..1911
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(2215..2232)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2215..2231)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2224..2246)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2440..2459)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2559..2581
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2619..2637)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2705..2809
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2810..2878,2879..3670)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3028..3047)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3835..4380
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     4281..4300
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4497..4514
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4664..4683
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4664..4682
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4915..4939
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    10718..10751
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        10815..11314
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     promoter        11326..11373
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11392..12195
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11455..11474)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12065..12084
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    12233..12457
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    12512..12545
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     enhancer        12581..12960
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12962..13239
                     /label=chicken beta-actin promoter
     intron          13240..14256
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     14180..14202
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     14264..14283
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(14316..14349)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    14482..14603
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    14715..14836
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    14948..15069
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(15092..15125)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             15147..15524
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     CDS             join(15543..15545,15546..16256)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             15543..16256
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(15573..15601)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     16196..16216
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             16278..16331
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             16347..17777
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(17835..17855)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(18253..18264)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    18396..18620
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(23695..23719)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggaagcaaaa agcctctcca cccaggcctg gaatgtttcc acccaatgtc gagcagtgtg
       61 gttttgcaag aggaagcaaa aagcctctcc acccaggcct ggaatgtttc cacccaatgt
      121 cgagcaaacc ccgcccagcg tcttgtcatt ggcgaattct accgggtagg ggaggcgctt
      181 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
      241 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
      301 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
      361 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
      421 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
      481 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
      541 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
      601 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
      661 tcgacctgca ggtcctcgcc atggatcctg atgatgttgt tgattcttct aaatcttttg
      721 tgatggaaaa cttttcttcg taccacggga ctaaacctgg ttatgtagat tccattcaaa
      781 aaggtataca aaagccaaaa tctggtacac aaggaaatta tgacgatgat tggaaagggt
      841 tttatagtac cgacaataaa tacgacgctg cgggatactc tgtagataat gaaaacccgc
      901 tctctggaaa agctggaggc gtggtcaaag tgacgtatcc aggactgacg aaggttctcg
      961 cactaaaagt ggataatgcc gaaactatta agaaagagtt aggtttaagt ctcactgaac
     1021 cgttgatgga gcaagtcgga acggaagagt ttatcaaaag gttcggtgat ggtgcttcgc
     1081 gtgtagtgct cagccttccc ttcgctgagg ggagttctag cgttgaatat attaataact
     1141 gggaacaggc gaaagcgtta agcgtagaac ttgagattaa ttttgaaacc cgtggaaaac
     1201 gtggccaaga tgcgatgtat gagtatatgg ctcaagcctg tgcaggaaat cgtgtcaggc
     1261 gatctctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
     1321 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     1381 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     1441 tggaatgcag atcctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     1501 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     1561 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     1621 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     1681 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgaggggg
     1741 ggcccggtac ccagcttttg ttccctttag tgagggttaa ttgcgcgcac gtttgcccgg
     1801 gagatggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     1861 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
     1921 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     1981 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     2041 tcgcagccaa cgtcggggcg gcaggccctg ccatagccac tggccccgtg ggttagggac
     2101 ggggtccccc atggggaatg gtttatggtt cgtgggggtt attattttgg gcgttgcgtg
     2161 gggtcaggtc cacgacccaa gcttggctgc aggtcgagct cgcgaaagct tggcactggc
     2221 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     2281 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     2341 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     2401 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     2461 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2521 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2581 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     2641 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     2701 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2761 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2821 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2881 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     2941 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3001 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     3061 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3121 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3181 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3241 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3301 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat
     3361 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3421 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3481 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3541 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     3601 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3661 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3721 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3781 ttaacgtgag ttttcgttcc actgagcgtc agacccctta ataagatgat cttcttgaga
     3841 tcgttttggt ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccgcct tgcagggcgg
     3901 tttttcgaag gttctctgag ctaccaactc tttgaaccga ggtaactggc ttggaggagc
     3961 gcagtcacca aaacttgtcc tttcagttta gccttaaccg gcgcatgact tcaagactaa
     4021 ctcctctaaa tcaattacca gtggctgctg ccagtggtgc ttttgcatgt ctttccgggt
     4081 tggactcaag acgatagtta ccggataagg cgcagcggtc ggactgaacg gggggttcgt
     4141 gcatacagtc cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga
     4201 gacaaacgcg gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag
     4261 cgcacgaggg agccgccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4321 caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa
     4381 aacggctttg ccgcggccct ctcacttccc tgttaagtat cttcctggca tcttccagga
     4441 aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg
     4501 agtcagtgag cgaggaagcg gaatatatcc tgtatcacat attctgctga cgcaccggtg
     4561 cagccttttt tctcctgcca catgaagcac ttcactgaca ccctcatcag tgccaacata
     4621 gtaagccagt atacactccg ctagcaacct ggcttatcga aattaatacg actcactata
     4681 gggagaccgg cagatcctaa ctataacggt cctaaggtag cgaaatcgat gcgacgctag
     4741 cggccgcgtt tgcttttgtt tcgtgttggt caccacggcc gagtttcctc gggaccccgg
     4801 gcagctgctt tacatcccaa agacctatct gctcggccgg cctcggaacg cgagcctgcc
     4861 cggaagatcc ccgggtaccg agctcgaatt catcgtcacc atcacctcga atcaacaagt
     4921 ttgtacaaaa aagcaggctt taaaggaacc aattcagtcg agcaaataga atttaaggcc
     4981 agcctggggc cttgtgaggg cctactgtga ccctccaaaa aataaaacaa gattagctgt
     5041 tgtgggaaag tagaaggaca aatgtgaggc aggagatgag agaggaatga ctggtcacat
     5101 gatgggagtc cagtgaacat gctggcaggt ctgtaaattg ttctcttatg ggtttatttg
     5161 tgatgataca tctagcttta ccagtgggtt gggatttttg tttttgtttt taactgatac
     5221 agacttatcc aatcaaaact gatagacaag gggactagaa tagttctgtg cttcaggact
     5281 ccatatgaaa ccctgcacta aatatcccaa aggctcttgc tttagagaaa ctccagaagg
     5341 aattttgttc atgagttagg ccctgtctca tcctttataa aattcattat aaaattatat
     5401 aatttcatta taaaataaac aagaacaaag gatccttaat ggtaagttct agtaaggtgt
     5461 acgataattg agttttcatg tatgtaattt taaaagaaaa atacaaggaa caagtggaat
     5521 cataggttct gaggtctctg aaacatctct tccccattac taaggtatat ttccagccat
     5581 gctcttccct cttcttagcc ctatttcagg gttgagaaac atgagaacac acacaggccc
     5641 attttacagg gtggcaaaga tggattgaag ttcttgggac aatgactact tgcccctttg
     5701 tgttctcttg tagtagctat aggtcagaac attagaatta cctctgccca agtaaacaaa
     5761 atgggaactc tttaccctgt tgaagatggc ttcacctggt aaagctaggc ttaagagcga
     5821 gtgctgcata gcccagtggt caagtgctcc aaagcatatg taagacccta ggcttaattc
     5881 ccagtgtcct gaaaagataa agcactggct tagtgttagg caaaggcact tgagtgtgct
     5941 gatctctgcc tcttctctgc agctttctcc ttccttcacc cctacctcag tgatccggaa
     6001 gatgtatgaa agcagagaga aaaccaagga ggaaatggca cctggaatgg tggttcctgg
     6061 tgatggtaaa gaggatactc agaagaccag cgaaggtact gctgcttacc tgtgtcagaa
     6121 cataggcatg agtgactgca gtgtcaggga gatgggttag tgagctcaaa cttgctcact
     6181 tgacattgtg gccagccggg gtaacgatgt gctttagctt atgattctta ccatctttca
     6241 agttgaataa caaactcatt tagcatattg atatagctca attctttttt taacgtattt
     6301 gtgtatttgc ctgtggtatg tctgcatcat gtgtatccag tgcccaagga ggccagaaga
     6361 gggcatcaga acctctagaa ttgtgagcca catgtgggtg ctgggaagag gtcctcttca
     6421 agagtatcca ggtagatagg gggatagatg cagggttgtt gttgtttctg ttacaggttg
     6481 tttgttttat ttgcaacagg atcttactgt gtagctgtgg ctaacctcaa actcacagag
     6541 atctactggt ctctgcctcc caagtcttag gattaaaggt gcctcaacta catgcctggc
     6601 aacttgtgct ttttgttttg taacactatg tcagaggttt ttgagttatt cctttatgca
     6661 ccgggaatgc agctcaattg tactaagtag tagaatactt gccagcatgt acaagaccat
     6721 gggttcatgt tcactcccca agaaaggaaa tgacaacatc aaaaataatt atttcagcaa
     6781 agaatttgaa gaccttggca tctatggact ggagagatag atcagggcac tgtttgctct
     6841 tccagaggac ctgggttcaa ttgccagcac ccacatgata gctcaccttt tgtgacttca
     6901 gccctaggtg acctagcacc ctcccctggc ctctgtgagc actgaactcg acaggcacac
     6961 agacatacat acaggcaaaa caccatccat atacatcata atataagaaa ataaggttat
     7021 aaataaaatg tgggcatcta ttcctagctc aaggcctaac tactctatga ccataactca
     7081 actccggggc ttatggagag taccagctgt gcatctgtct acatttaaag caaatagatg
     7141 actttccatg aaagcatcta acatggttaa gaggaaaaaa gagccggcat ggtggcacac
     7201 gcctttaatc ccagcacttg ggaagcatag gcaggtggat ttctgagttc gaggccagcc
     7261 tggtctacaa agtgagttcc aggacagcca gggctataca gagaaaccat gtcttgaaaa
     7321 accaaaaaaa aaaaaaagga aaaaagaatt gtcagaattt ttaaaagtaa gatttgctgg
     7381 gttgttggga attgaaataa attgataaag gatatcaaaa tgtcctgtaa atgaatcctt
     7441 acagagaacc tgctgtcatc caatcccata cccaatactg atcaagactc ttctacgaca
     7501 aatcctaagc tttcaacatt acagcggtct tcatgttcca ccccactctc ccagaccagc
     7561 cgttatacca aagaacaaga ttatcgacct aaaacagcag gaaggaaaac acctaccttg
     7621 gcatccccag tcccaggaac accttttctc cgccctaccc accaagttcc ccttgtccct
     7681 catgttccta ttgttcgacc tgcacaccag cttcacccag gattagtcca aaggttgata
     7741 gcccaagggg tacatccaca gcatcttcca agtttgctcc aagctggtga gtttctgact
     7801 gggtgtgggc attggttatt ggtcgtagta taaaaattgc tgccactaat taaatgttac
     7861 attatactaa gtattccaaa aagatcttac tagatttaaa ttcttctacc taagtagtga
     7921 gtccaatcca gtctcacttc cttagctcac aaaaagatta ggaaacctgc cagacagtgg
     7981 taatacacgc ctttaatccc tgcactcagg aagcagaggc aggtggatct ctttgagttc
     8041 aaggatagcc agggtacact aaggaaccct ggctgtggcg ttcatttaca attgtgtctg
     8101 tgtatgcata ttcatgtacc ctggtacatg tatggtggca agtgttcacc acatccacct
     8161 ccttgtaagt atccaaatca acaaaagtag taaactttga catttttagg aacatctaca
     8221 actcatgagt ttcttctttc tccccatcag gtgtgcttcc tcctgggata gacatggctc
     8281 ctttacaggg gctatctggc ccactcctgg gtcaaccctt gtaccctttg gtctctgctg
     8341 ctagtcaccc tctcctaaac cctcgtcctg ggacccctct gcatttggcg gtgatgcagc
     8401 agcagctaca gcgctcaggt aaggtttaga aataggccag gttagctgat cacactggaa
     8461 agttctgaga tttccaggag caatagttat cactgcccct ttgtttctta ggtgtcacta
     8521 gaaaaacagg tgaagtgaac tgacctgtgc agtctcaggg attattacta ctgcaaggac
     8581 agtcggagcc agccatgctc tagctctgtt tttcacttct tataagcctg agaatttttg
     8641 ctgagatgtg aacatgtcag ccttggtggg ctttcttacg ttgcattttc ttacgttgca
     8701 ttttgcactt taggaagcct ttggttaaat cctctggtag acttcccatc ccccaaagta
     8761 acaaaccatt caagcaaagt aggtcagaaa atattgtcaa tactgagaac aggacttggg
     8821 agtgagtatc caagggtggt cttagtagac ttgtggggtt ctgtgggtga ggtgtagagt
     8881 agagctactt tgctgcactg acactcttct ctccatagtt ctgcatcctc caggctctag
     8941 ttcccaggca gcagctatca gcgttcagac tcctcagaat gtacccagcc ggtcgggcat
     9001 gccccacatg cactcccagc tggagcatcg taccagccaa aggagcagct cccctgtggg
     9061 ccttgccaaa tggtttggct cagatgtgct acagcagcct ctgccctcca tgcccaccaa
     9121 agtcatcagt gtagatgaac tggaatatag acagtgaaga gggcaggctg gctcacccat
     9181 acctggacct gtggtgacac cctggtcatg actctcattc cctctttgta atgggctttt
     9241 acattggagc acactatgtg aagatgttta ggggatccac atacctacca tgatctacat
     9301 tatgacagaa ggctgttaaa tcgaatgaac ctacatggtt caaatacaag ggatacaaga
     9361 ttgtcagtcc tggaagtctt tcttttataa aatatgtgaa tgaagtgttg gtgtcttcta
     9421 gaggtgacac ctaagggttc tgaaaaaata aaatgtatag acccttatgt acagacctgt
     9481 gtataaactt ttgtacatac aaatagggta gctttttttg aacttataca tacagctgta
     9541 cataaagtaa ctatcagtta ggcttgtgtc aactgtttgg atttttttca cttgaatatt
     9601 tgggactttt tcttttggtt tattaaaagt tacatatgcc acgtgtgtga acgatatggc
     9661 tggtactgtg tttatttctt ccatgaacta agacagtcta aatgagttcc tttcacgttt
     9721 taattttacc ttaggacttc tggaatttct tctgcacata aagttctgat agcattagtt
     9781 taagctggac taaccctgaa agtagcttgt ggcaagtatc aaggaatcaa tattatactc
     9841 tacaaaatca aagtttacag agaagtcata tagtaatttt tctgaaattt actggcacaa
     9901 tgttaatcca gcctgactcc aactaattaa tggtcacatt aatttaagtc tttcccttgc
     9961 ctctgctgca ttagtttctc tcaaaattgt taacttacaa cttgaagtct ggtattataa
    10021 attgaatgta aagcattctg aaagatacta tactgattgc aggtttttca gtcaggttca
    10081 agctaatttg accagtcatt ggattaatta tggatctggg gccataaatg ctattttaat
    10141 tccactatag agattaaaat aagccattct ccatttcata atattctatt ggactttgac
    10201 tgcaggggcc tccaagtctt gacagtagat tataatcctt cagctgccca ctctactgga
    10261 ggaggacaaa ctggtcactt ttcagcaaaa cctggctgtg gatcagggca gtctggtact
    10321 tccaagctca ttagatgcca tcatgctctc actgcctcct cagcttcaag aggaatctgg
    10381 aaaaagcagt cccactggtc aggaaaggaa cactagtgca cttatcctgg gtgtctgctg
    10441 agccaacagt ggtagtaagg taagggcagg atgtgtcaaa ctgccaatag agaactactt
    10501 actcttcagg ctgaagctga tggaacaggt aacaaaggca aacactaatc atgatcagca
    10561 agatgaagca gaaagggaac aaggggatat taaatgtgta tagacacgct agagagatgg
    10621 ctcagcagtt aagagaacta gctggtcttt cagaggtcct gagatcaatg gcgcgccagt
    10681 cttaattaag gtatcgataa gcttgatatc gaattccgaa gttcctattc tctagaaagt
    10741 ataggaactt caggtctgaa gaggagttta cgtccagcca agctagcttg gctgcaggtc
    10801 gtcgaaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
    10861 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
    10921 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
    10981 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
    11041 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
    11101 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
    11161 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
    11221 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
    11281 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcctgttga caattaatca
    11341 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catgggatcg
    11401 gccattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
    11461 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
    11521 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
    11581 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
    11641 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
    11701 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
    11761 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
    11821 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
    11881 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
    11941 ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
    12001 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
    12061 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
    12121 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
    12181 gacgagttct tctgagggga tcaattctct agagctcgct gatcagcctc gactgtgcct
    12241 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
    12301 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
    12361 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
    12421 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
    12481 tggggctcga ctagagcttg cggaaccctt cgaagttcct attctctaga aagtatagga
    12541 acttcatcag tcaggtacat aatggatccg gcgcgccgtc gacattgatt attgactagt
    12601 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
    12661 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
    12721 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
    12781 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
    12841 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
    12901 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
    12961 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
    13021 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
    13081 gggcgcgcgc crggsggggs ggggsggggs grggggsggg gsggggcgag gcggagaggt
    13141 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
    13201 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
    13261 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
    13321 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
    13381 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
    13441 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
    13501 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
    13561 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
    13621 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
    13681 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
    13741 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
    13801 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggrg
    13861 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
    13921 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
    13981 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
    14041 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
    14101 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
    14161 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
    14221 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
    14281 gtgctgtctc atcattttgg caaagaattg ctagcataac ttcgtatagc atacattata
    14341 cgaagttata gccaccgcgg tggcggccgc tctagaatca taatcagcca taccacattt
    14401 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
    14461 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
    14521 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    14581 tccaaactca tcaatgtatc ttaactagaa tcataatcag ccataccaca tttgtagagg
    14641 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
    14701 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    14761 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    14821 tcatcaatgt atcttaacta gaatcataat cagccatacc acatttgtag aggttttact
    14881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    14941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    15001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    15061 tgtatcttaa ctagtggatc tcatgcctag gataacttcg tatagcatac attatacgaa
    15121 gttataggcg cgccggtacc gccaccatgc ctgaaccctc taagtctgct ccagccccta
    15181 aaaagggttc taagaaggct atcactaagg cgcagaagaa ggatggtaag aagcgtaagc
    15241 gcagccgcaa ggagagctat tctatctatg tgtacaaggt tctgaagcag gtccaccccg
    15301 acaccggcat ctcatccaag gccatgggga tcatgaactc cttcgtcaac gacatcttcg
    15361 agcgcatcgc gggcgaggct tctcgcctgg ctcactacaa taagcgctcg accatcacct
    15421 ccagggagat tcagacggct gtgcgcctgc tgctgcctgg ggagctggct aagcatgctg
    15481 tgtccgaggg cactaaggca gttaccaagt acactagctc taaggatcca ccggtcgcca
    15541 ccatgagcaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
    15601 gtgatgttaa tgggcacaaa ttttctgtcc gtggagaggg tgaaggtgat gctacaaacg
    15661 gaaaactcac ccttaaattt atttgcacta ctggaaaact acctgttccg tggccaacac
    15721 ttgtcactac tctgacctat ggtgttcaat gcttttcccg ttatccggat cacatgaaac
    15781 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt
    15841 tcaaagatga cgggacctac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg
    15901 ttaatcgtat cgagttaaag ggtattgatt ttaaagaaga tggaaacatt cttggacaca
    15961 aactcgagta caactttaac tcacacaatg tatacatcac ggcagacaaa caaaagaatg
    16021 gaatcaaagc taacttcaaa attcgccaca acgttgaaga tggttccgtt caactagcag
    16081 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
    16141 acctgtcgac acaatctgtc ctttcgaaag atcccaacga aaagcgtgac cacatggtcc
    16201 ttcttgagtt tgtaactgct gctgggatta cacatggcat ggatgagctc tacaaaggcg
    16261 gctccggcgg ccgctccgag ggccgcggca gcctgctgac atgcggcgac gtggaagaga
    16321 accctggccc tgctcctggc agcacaatgg tgagcaaggg cgaggaggtc atcaaagagt
    16381 tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg
    16441 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
    16501 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg
    16561 cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct
    16621 tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact
    16681 cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc
    16741 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt
    16801 acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg
    16861 gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg
    16921 gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg
    16981 tggaacagta cgagcgctcc gagggccgcc accacctgtt cctggggcat ggcaccggca
    17041 gcaccggcag cggcagctcc ggcaccgcct cctccgagga caacaacatg gccgtcatca
    17101 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
    17161 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
    17221 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
    17281 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
    17341 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
    17401 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
    17461 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
    17521 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
    17581 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
    17641 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
    17701 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg
    17761 acgagctgta caagtaaggc gcgccaacct ctggattaca aaatttgtga aagattgact
    17821 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
    17881 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
    17941 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
    18001 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
    18061 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
    18121 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
    18181 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
    18241 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
    18301 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
    18361 gcctccccgc actagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
    18421 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
    18481 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
    18541 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
    18601 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct cgactagagc
    18661 ttgcggaacc cttaattaat ttagacaccc acatggtggc tcatgaccat ctatctataa
    18721 atggatctga ttttcatgtc tggcagtgta cagaagctaa ctgaagaaag gtggaagacc
    18781 cacaagagtt caagataagc cctatatagt gaagttcaag gcaagctttt tctacctgaa
    18841 acttagtctc aaaaaaaaat gaatacgtaa acagtcttcc aggggataag aaccttacag
    18901 aaaaagcaga aatgcctggg gcactggatt accgatgtaa tcaaattcag tccttgaatt
    18961 gaacacagga ttgcctagag caaggccagc cagagattca tctcagaggg agaaaggtgt
    19021 ctttggagca attttgtggt aatctagtat gtatcacata agtttagacg catttgggac
    19081 tggaaagatg tgaacaaagc accctatggc tcacatctgt cattaactct agttccagtg
    19141 cacctgacac cgtcttctgg cctctgcagt gaccaagcac atgggtagta tgtagacata
    19201 tacataagca aaacacacat cattaaaaag tgacatttcc caaaggaagc tgaagaacca
    19261 gttcttgaga agatagtaga aatcagaagg ggaaatagta gacatacaga gggactgacc
    19321 aggttgtgtc acctttatag gctaggctaa tggatgatcg acactagcgc tctttgtgaa
    19381 ggacacacaa atgagacata gtttatagga ctaaacacac ttctaagcaa tttaatgaga
    19441 cttaagaccc tgtctctagc aaatactctg gatgatattc agctcaaggc tcttgtcaga
    19501 catgtttcca ttttcaaggt gagctaactg gccaaaactg ccaacaacct gtagtgaata
    19561 gagaagatga gaaaatctgg attctcaaat gacctaatga aagccactgg agcgccatat
    19621 ggtttctgtg aaaatgcctt ttcaatcatt aacctcttaa atgagtgtta gcatcctaac
    19681 taatgagtgg tgcagaatag tgggtctgct tagcttaact aaggccaaga aaacaaaaca
    19741 ggaaattcat tccatgtcat gagactcata ctacgaggtt cccttagacc tcaggagaaa
    19801 aagtctttgg ctgtaagaac acacctcagt ggatgtggta gactatgcct ttactctttt
    19861 tttttttttt tttttttttt gggttttttc gagacagggt ttctctgtgt agccttggct
    19921 gtcctggaac tcactttgtt gaccaggctg gcctcgaact cagagatccg cctgcctctg
    19981 cctctcaagt gctagaatta aaagtgtgcg ccaccactcc cagctatata gactatgcct
    20041 ttaatcagga cacttgggag gcagggggat ctcaaattca attccagaca gatgactgac
    20101 aaacacacac atacaccatt cgtcttttct ttttttcttt tttcttttga aaacaggttt
    20161 ctctatgcag ccctggcttt cctagaattc aatctataga cccagctggc ctcaaacaca
    20221 gaacctcttg cctctgcctc ctcaacacta tgactaaagg tgtgaaccac caccaccacc
    20281 acccacctgg ccaaagaatg aattgagtga ataagtacga gtgcaactct ccagcagcct
    20341 gaggcaagaa agcttaacag ttgaacctga gaccctactt cgtttgtgtt gtacatatct
    20401 tctcataatg aactaggcag cctagtctct ctaccaaata caccatgcac ttacacctcc
    20461 tcaaaccaac tgcaacagct acacacattt ccatggcttg cctgtaataa taaattcaca
    20521 aacttcctct aagtaacttt tagtggaaat ctaccaggtt atgcctattt gtgattgggt
    20581 gcacagatga ctagatgaga tgtaatttgg gaagtgacat gcaataggaa aaatgaatat
    20641 ataacctata aatagaaaac accaaggcaa gcgtggtgac tcacaccttt aattagcact
    20701 tgggaggcag aagtcgcatc atctcttagg tcaaggccaa tctagttcca gaccagcaag
    20761 gacaacagaa accttgcctt gatataaaca ttttaaaact ttaaaaatag agaagcccaa
    20821 actctgctca ttggtaatct acccttcctt tctttagaca ccttgagaag ctaggctaca
    20881 tagctttggg tccttacgta cccccactca ctcagcctgc agtcaatctc tgatagtgtt
    20941 tcctgccata agctacacta ctgttttgct ccaaacctta cttctagtac aaatccatgt
    21001 gaggtcttcg tttcaatttg ggggggaaga ggccagaaca cttgccgcag atcccaacaa
    21061 cgggcagcac attcccagct agtgtatctg atacaaactc tatcttcatt cagaaccatc
    21121 aagtctaagc cccaagagca aaggtaaatt catgtatgtt tggctggaga aagcatttca
    21181 agtcaaggtg agccttgaac tcctaacact cttgcctcta ccttccaaat gccaatatag
    21241 gtatttgtca caccagcttc tcaaatcctg ttgggctagc aactgggtgg gaggagtgta
    21301 gaggggtact aaaaaatgga ctcaaggcct cttcaaaacc actgaggcct tgtacaggat
    21361 ttgaacttcc ttctcttttc agcatcctga agtacaaagc taaagcaaag taaatttttg
    21421 cttggcctga ggacttccaa aaatctaggc tctcactgga gaagaaacct agctggaaat
    21481 tccacagttc tgataagcca attctttttt tttttaacca tttatttatt tataagtaag
    21541 tacactgtag ctgtcttcag acatcccaga tctcatgacg gatggctgtg agctaccttg
    21601 tggttgctgg gattttaact caggaccttc agaagagcag tcagtgctct taaccagtgc
    21661 tctccggccc agtggaaacc aattctttag tgtggggaca ggctttcagt catagcctat
    21721 cagtcaatgt cctagtgaga aaccagtcta tattttgagg cttgagaggc ctgattccaa
    21781 ggacagtttt aaacccggag aaaggaagag agctagctct agggcagcag acggagccct
    21841 gagggaaggg tagtgtgtgg taaggcccac agctttcctc agacctctga aggctctagc
    21901 tcatctcctt tgaagacaga agaagcaaag tctgttctcc tacaaagacg ttcctgtctt
    21961 tctcaaggcc tcctctatca tcactgcaga ccacattaaa ataatcttcc agccaggcgg
    22021 tggtggcaca cacctttaat cccagcactt gggaggcaga ggcaggccgg atctctgagt
    22081 ttgaggccag cctggtctac aaagtgagtt ccaggacagc cagagctata cagagaaacc
    22141 ctgtctcgaa aaaccaaaaa aaaaaaaaaa aaaaaaaaaa aaagattact gtatttgtga
    22201 gcctcaatct catcgtgtcg tacatggaac agtggcatta acttttaagg ttgggattgc
    22261 tggtattaag gggagggttt tggaagctgc ttagcacagt gtcagtcgta ggaaatattc
    22321 actgtatggt ggttcttgtc actcagcctg tttttgtttt tgttttgttt tttgtttttt
    22381 tcctgagaca gggtttctct gtagtgttta tatatatata tatatatata acttcagaca
    22441 gcaaaagagg gtatcagaaa gggctaatag acaacttgca cctcacccta taggtggaac
    22501 aacagtatga actaaccagt accccccgga gctcgtgtct ctagctggat atatagcaga
    22561 agatggccta gatggccatc agtggaaaga gaggcccatt ggtcttacaa actttatgtg
    22621 cctcagtaca agggaacacc agggccaaga aagcgagggg aggggggttg ggagactttc
    22681 agagtagcat tggaaatata aatgaagaaa atacctcctc atcacagcag tccgctcagc
    22741 tcagaatcca gagactattg cgctggagaa aaatctgcag ttaactttac ctattatacc
    22801 ttcctctctc caatgtcctg agaaggacca cttgaaagga cagaaagcca agcccaggtg
    22861 aaagcctatc attacagcac actaccaatg gacaaacgga cacaggtccc attctctgtc
    22921 ccatttccta tgaccaagca ggcgttgcct cattcacctc atcacacagc aaacctcaca
    22981 tgaataacga cacatggcca gcgggcccac cagcttcttt atacagttca atagaagtga
    23041 cagcaaagta gggaaagtgg tgttgctgag gagctaaagg tgcacagtgg taagcccagc
    23101 actgaggaga tggaggctga tgaaagggat tctaggccag cctcagctgc aagagttcaa
    23161 agccagcctg aaatacataa caccctatct ccggaaagaa aaaaattgtg gtgtttcagt
    23221 ttgataagtc tccatatcaa tccctgtatc ccaatccaga ggctgccaaa gacagagggg
    23281 ctgaggaagg tacttagtca taaggaacac tgctgtggcc agccagcaga tggcaagagg
    23341 acttcacttc ttcacaatct gaatgacgtc ttcatcttcc aatgtgtggt ctttacccac
    23401 tttctgagga ttatgtttca cagacagacc ccagaccaaa gcactgtatg gagaggaata
    23461 aagagtcagt ggctgtagca agcagctgct tccctaactg cccaggtgcc agcttatcaa
    23521 atttagcatc ctggatctta aaaggcacag aactacaaca gataaaagcc ctcttctaaa
    23581 tacatcagaa taaacaaaca gtcaaagcca cttgctaaag cagtgttgca cactgagtca
    23641 tgggatcgct gtcattcaac agccaattag tttaaaccaa ctggagatat ctagacccag
    23701 ctttcttgta caaagtggtt gattcgaggc tgctaacaaa tcgagcagtg tggttttcaa
    23761 ga
//
