LOCUS       sequence-360527-        9989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/G2032R 
            mutation in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Lin JJ, Choudhury NJ, Yoda S, Zhu VW, Johnson TW, Sakhtemani R, 
            Dagogo-Jack I, Digumarthy SR, Lee C, Do A, Peterson J, 
            Prutisto-Chang K, Malik W, Hubbeling HG, Langenbucher A, Schoenfeld 
            AJ, Falcon CJ, Temel JS, Sequist LV, Yeap BY, Lennerz JK, Shaw AT, 
            Lawrence MS, Ou SI, Hata AN, Drilon A, Gainor JF
  TITLE     Spectrum of Mechanisms of Resistance to Crizotinib and Lorlatinib in
            ROS1 Fusion-Positive Lung Cancer.
  JOURNAL   Clin Cancer Res. 2021 May 15;27(10):2899-2909. doi: 
            10.1158/1078-0432.CCR-21-0032. Epub 2021 Mar 8.
  PUBMED    33685866
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/1078-0432.CCR-21-0032"; journalName: "Clin Cancer Res"; 
            date: "2021-05-15- 15"; volume: "27"; issue: "10"; pages: 
            "2899-2909"
COMMENT     Sequence Label: pLenti-CD74-ROS1 S1986F/G2032R
FEATURES             Location/Qualifiers
     source          1..9989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        103..306
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     257..277
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     303..327
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     402..421
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        402..420
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    450..474
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2623..2647)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2679..2702
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2718..2744
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2718..2744
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2751..3317
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2767..3319
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2911..2928)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3138..3157
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3331..3930
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3331..3350)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3827..3847
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3947..4535
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4000..4020)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4418..4429)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4610..4843
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4962..4980)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5048..5152
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5153..5221,5222..6013)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5371..5390)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6184..6772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6673..6692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6926..6943
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7183..7204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7219..7224,7225..7242,7243..7249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7257..7273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7262..7284
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7281..7297
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7281..7297
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7347..7366
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7558..8191
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8238..8363
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8860..9093
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9278..9322
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9619..9736
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        join(9788..9989,1..102)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
       61 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      121 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      181 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      241 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      301 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      361 catagaagac accggcggcc gcagatctct cgaggttgat ctaatacgac tcactatagg
      421 gagacccaag ctggctagtt aagctatcaa caagtttgta caaaaaagca ggctccgcgg
      481 ccgccccctt caccatgcac aggaggagaa gcaggagctg tcgggaagat cagaagccag
      541 tcatggatga ccagcgcgac cttatctcca acaatgagca actgcccatg ctgggccggc
      601 gccctggggc cccggagagc aagtgcagcc gcggagccct gtacacaggc ttttccatcc
      661 tggtgactct gctcctcgct ggccaggcca ccaccgccta cttcctgtac cagcagcagg
      721 gccggctgga caaactgaca gtcacctccc agaacctgca gctggagaac ctgcgcatga
      781 agcttcccaa gcctcccaag cctgtgagca agatgcgcat ggccaccccg ctgctgatgc
      841 aggcgctgcc catgggagcc ctgccccagg ggcccatgca gaatgccacc aagtatggca
      901 acatgacaga ggaccatgtg atgcacctgc tccagaatgc tgaccccctg aaggtgtacc
      961 cgccactgaa ggggagcttc ccggagaacc tgagacacct taagaacacc atggagacca
     1021 tagactggaa ggtctttgag agctggatgc accattggct cctgtttgaa atgagcaggc
     1081 actccttgga gcaaaagccc actgacgctc caccgaaaga tgatttttgg ataccagaaa
     1141 caagtttcat acttactatt atagttggaa tatttctggt tgttacaatc ccactgacct
     1201 ttgtctggca tagaagatta aagaatcaaa aaagtgccaa ggaaggggtg acagtgctta
     1261 taaacgaaga caaagagttg gctgagctgc gaggtctggc agccggagta ggcctggcta
     1321 atgcctgcta tgcaatacat actcttccaa cccaagagga gattgaaaat cttcctgcct
     1381 tccctcggga aaaactgact ctgcgtctct tgctgggaag tggagccttt ggagaagtgt
     1441 atgaaggaac agcagtggac atcttaggag ttggaagtgg agaaatcaaa gtagcagtga
     1501 agactttgaa gaagggtttc acagaccagg agaagattga attcctgaag gaggcacatc
     1561 tgatgagcaa atttaatcat cccaacattc tgaagcagct tggagtttgt ctgctgaatg
     1621 aaccccaata cattatcctg gaactgatgg agggaagaga ccttcttact tatttgcgta
     1681 aagcccggat ggcaacgttt tatggtcctt tactcacctt ggttgacctt gtagacctgt
     1741 gtgtagatat ttcaaaaggc tgtgtctact tggaacggat gcatttcatt cacagggatc
     1801 tggcagctag aaattgcctt gtttccgtga aagactatac cagtccacgg atagtgaaga
     1861 ttggagactt tggactcgcc agagacatct ataaaaatga ttactataga aagagagggg
     1921 aaggcctgct cccagttcgg tggatggctc cagaaagttt gatggatgga atcttcacta
     1981 ctcaatctga tgtatggtct tttggaattc tgatttggga gattttaact cttggtcatc
     2041 agccttatcc agctcattcc aaccttgatg tgttaaacta tgtgcaaaca ggagggagac
     2101 tggagccacc aagaaattgt cctgatgatc tgtggaattt aatgacccag tgctgggctc
     2161 aagaacccga ccaaagacct acttttcata gaattcagga ccaacttcag ttattcagaa
     2221 attttttctt aaatagcatt tataagtcca gagatgaagc aaacaacagt ggagtcataa
     2281 atgaaagctt tgaaggtgaa gatggcgatg tgatttgttt gaattcagat gacattatgc
     2341 cagttgcttt aatggaaacg aagaaccgag aagggttaaa ctatatggta cttgctacag
     2401 aatgtggcca aggtgaagaa aagtctgagg gtcctctagg ctcccaggaa tctgaatctt
     2461 gtggtctgag gaaagaagag aaggaaccac atgcagacaa agatttctgc caagaaaaac
     2521 aagtggctta ctgcccttct ggcaagcctg aaggcctgaa ctatgcctgt ctcactcaca
     2581 gtggatatgg agatgggtct gattaaaagg gtgggcgcgc cgacccagct ttcttgtaca
     2641 aagtggttta gctagagggc ccgcggttcg aactcgagga ctacaaggac gacgatgaca
     2701 agtcggccgc tggaggatac ccctacgacg tgcccgacta cgcctagaat tccgcccccc
     2761 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     2821 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     2881 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     2941 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     3001 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     3061 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     3121 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     3181 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     3241 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     3301 gaaaaacacg atgataatat ggccacaacc atgaccgagt acaagcccac ggtgcgcctc
     3361 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     3421 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     3481 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     3541 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     3601 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     3661 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     3721 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     3781 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     3841 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     3901 tggtgcatga cccgcaagcc cggtgcctga tgcatcgatg gatcctaatc aacctctgga
     3961 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     4021 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     4081 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     4141 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     4201 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     4261 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     4321 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     4381 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     4441 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     4501 gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag accaatgact
     4561 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     4621 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     4681 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     4741 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     4801 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     4861 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggcccggg
     4921 ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga tacgcctatt
     4981 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     5041 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5101 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5161 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5221 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5281 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5341 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     5401 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5461 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5521 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5581 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5641 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5701 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5761 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5821 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5881 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5941 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6001 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6061 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6121 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6181 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6241 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6301 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6361 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6421 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6481 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6541 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6601 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6661 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6721 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6781 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6841 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6901 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     6961 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca agctcatggc
     7021 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7081 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc cgtggcacga
     7141 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     7201 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     7261 gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt tcacaaataa
     7321 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7381 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     7441 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     7501 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     7561 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     7621 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     7681 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     7741 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     7801 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     7861 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     7921 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     7981 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     8041 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8101 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8161 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     8221 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     8281 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     8341 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     8401 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     8461 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     8521 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     8581 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     8641 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     8701 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     8761 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     8821 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     8881 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     8941 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     9001 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     9061 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     9121 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     9181 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     9241 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     9301 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     9361 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     9421 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     9481 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     9541 agacagatcc attcgattag tgaacggatc tcgacggtat cgccgaatta attcacaaat
     9601 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggaa
     9661 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     9721 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggactagtgg
     9781 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     9841 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     9901 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     9961 atatgccaag tacgccccct attgacgtc
//
