LOCUS       sequence-360528-       11896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses bfp-hcoilin in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11896)
  AUTHORS   Laprade H, Querido E, Smith MJ, Guerit D, Crimmins H, Conomos D, 
            Pourret E, Chartrand P, Sfeir A
  TITLE     Single-Molecule Imaging of Telomerase RNA Reveals a 
            Recruitment-Retention Model for Telomere Elongation.
  JOURNAL   Mol Cell. 2020 Jul 2;79(1):115-126.e6. doi: 
            10.1016/j.molcel.2020.05.005. Epub 2020 Jun 3.
  PUBMED    32497497
REFERENCE   2  (bases 1 to 11896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.05.005"; journalName: "Mol Cell"; date: 
            "2020-07-2- 2"; volume: "79"; issue: "1"; pages: "115-126.e6"
COMMENT     Sequence Label: pHage2-EF1a-TagBFP-hCoilin
FEATURES             Location/Qualifiers
     source          1..11896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3456..4154
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    6812..7273
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6868..6885)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7095..7114
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7278..8303
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7278..8303
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    8338..8926
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8391..8411)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8809..8820)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9074..9254
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9301..9319)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9387..9491
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9492..9560,9561..10352)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9710..9729)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10523..11111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11265..11282
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11522..11543
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11558..11563,11564..11581,11582..11588)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11596..11612
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11601..11623
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11620..11636
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11620..11636
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11686..11705
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagcttcagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc ttaattaagt
     3421 ttaaacggat cccgccggcg gctagcgcta ccggtatgag cgagctgatt aaggagaaca
     3481 tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg
     3541 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
     3601 gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct
     3661 tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca
     3721 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
     3781 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca
     3841 acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc
     3901 ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc
     3961 atctgatcgc aaacatcaag accacatata gatccaagaa acccgctaag aacctcaaga
     4021 tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga
     4081 cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg
     4141 ggcacaagct taataagtac tcagatccac caagcaagat ggcagcttcc gagacggtta
     4201 ggctacggct tcaatttgat tacccgccgc cagctacccc gcactgtacg gccttctggc
     4261 ttctggtcga cttgaacaga tgccgagtcg tcacagatct cattagtctc atccgccagc
     4321 gcttcggctt cagttctggg gccttcctag gcctctacct ggaggggggg ctcttgcccc
     4381 ccgccgagag cgcgcgcctt gtgagagaca acgactgcct cagagttaaa ttagaagaga
     4441 gaggagttgc tgagaattct gtagtcatca gtaatggtga cattaattta tctcttagaa
     4501 aagcaaagaa gcgggcattt cagttagagg agggtgaaga aactgaacca gattgcaaat
     4561 attcaaagaa gcattggaag agtcgagaga acaataacaa taatgagaag gtcttggatc
     4621 tggaaccaaa agctgtcaca gatcagactg tcagcaaaaa aaacaagaga aaaaataaag
     4681 caacctgtgg cacagtgggt gatgataacg aagaggccaa aagaaaatca ccaaagaaaa
     4741 aggagaaatg tgaatataaa aaaaaggcta agaatcccaa gtctccgaaa gtacaggcag
     4801 tgaaagactg ggccaatcag agatgtagtt ctccaaaagg ttctgctaga aacagccttg
     4861 ttaaagccaa aaggaaaggt agtgtaagcg tttgctcaaa agagagtccc agttcctcct
     4921 cggagtctga gtcttgtgat gaatctatca gtgatggtcc cagcaaagtc actttggagg
     4981 ccagaaattc ctcagagaaa ttaccaactg agttatcaaa ggaagaaccc tctaccaaaa
     5041 atacaactgc agacaaactg gctataaaac ttggctttag ccttaccccc agcaagggca
     5101 agacctctgg aacaacatct tccagttcag actctagtgc agagtcagac gaccaatgct
     5161 tgatgtcatc gagcaccccg gagtgtgctg cgggtttctt aaagacagta ggcctttttg
     5221 caggaagagg tcgtccaggc ccagggctgt catcacagac tgcaggtgct gctggatgga
     5281 ggcgttctgg ctcaaatggt ggtggacagg ctcctggtgc ttctcccagt gtgtctctcc
     5341 ctgctagttt aggaagagga tggggtagag aagagaacct tttttcttgg aagggagcta
     5401 agggacgggg catgcggggg agaggtcgag gacgagggca tcctgtttcc tgtgttgtaa
     5461 atagaagcac tgacaaccag aggcaacagc aattaaatga cgtggtaaaa aattcatcta
     5521 ctattatcca gaatccagta gagacaccca agaaggacta tagtctgtta ccactgttag
     5581 cagctgcccc tcaagttgga gaaaagattg catttaagct tttggagcta acatccagtt
     5641 actctcctga tgtctctgac tacgaggaag gaagaatatt aagccacaat ccagagaccc
     5701 agcaagtaga tatagaaatt ctttcatcct tacctgcctt gagagaacct gggaaatttg
     5761 atttagttta tcacaatgaa aatggagctg aggtagtgga gtacgctgtg acacaggaga
     5821 gcaagatcac tgtattttgg aaagagttga ttgacccaag actgattatt gaatctccaa
     5881 gtaacacatc aagtacagaa cctgcctgag tatgacctct ccacctcata gtttatgaat
     5941 gtcttgtttg tgaaagtgac tataacccaa actttttttt tttttaaaga ggatttggaa
     6001 gttgtatgga tttttttgtt atcttcactt tactgcatag gaaacaatct acctcatcat
     6061 ttaaaatgac atgggtgtcg gttttgtaga tctttggttt ttttgtcagg tttaatttca
     6121 gttaacaaaa tgtaaaacat gacattccct gcagatattg ttgtatgcca gtatggtttc
     6181 ttctctttct ttaaatgttt ttggccatca agtagcagtc gtcagtagga gtttataata
     6241 ccaagaatgt gctgcgtatc ttgtctcaat aagttttaag taacatttaa aaatattaaa
     6301 gcatgttatt tgacctaatt ttttagcatt tgagttgttc cattaaatgg agcatcttgt
     6361 aaatttcaag tattttatac ttgcaattgt taagagttaa caggtagttg gatttgtcgc
     6421 agacaatgag ttaaggaatc ctttcacgtt tttcccaact ttaaaattaa ggattctcag
     6481 gaccctgtgt agagcagtga aaataagatg tgcgtatgtg tgtgtatgcc tggagaattg
     6541 gtgtttcact tcagtgagag gattggctgt gagcttcaga ccaggaaatg tgtcatcttg
     6601 ccaggcacct ggctgagtgt gctggagtga ggatcttgaa cagaaacttc cttttctgtt
     6661 attattcact acgaagctaa aatggccaaa tatataccgt gaaaattggt ttcatttaac
     6721 aaaagatcag atccctcctt cagctgtatc tagagctcga ggtcgaccaa ttccggttat
     6781 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     6841 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     6901 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     6961 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     7021 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     7081 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     7141 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     7201 agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     7261 aacacgatga taataccatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     7321 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     7381 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     7441 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     7501 cggaagtgct tgacattggg gaatttagcg agagcctgac ctattgcatc tcccgccgtg
     7561 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
     7621 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     7681 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     7741 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     7801 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     7861 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     7921 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     7981 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     8041 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
     8101 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     8161 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     8221 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     8281 ctcgtccgag ggcaaaggaa tagagtagat gccgaccggg atctatcgat agatcctaat
     8341 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     8401 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     8461 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     8521 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     8581 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     8641 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     8701 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     8761 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     8821 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     8881 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag
     8941 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     9001 ggaagggcta attcactccc aacgaagaca agatcacctg caggacaggc gcgccctgct
     9061 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     9121 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     9181 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9241 gaaaatctct agcacccggg cgattaagga aagggctaga tcattcttga agacgaaagg
     9301 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     9361 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     9421 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     9481 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     9541 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     9601 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     9661 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     9721 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     9781 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     9841 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     9901 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     9961 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    10021 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    10081 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    10141 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    10201 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    10261 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    10321 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    10381 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    10441 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    10501 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    10561 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    10621 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    10681 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10741 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    10801 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    10861 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    10921 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    10981 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    11041 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11101 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    11161 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    11221 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    11281 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    11341 aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    11401 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
    11461 aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    11521 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    11581 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga
    11641 ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    11701 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
    11761 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
    11821 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
    11881 ctacaaactt agtagt
//
