LOCUS       sequence-360529-        9989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CD74-ROS1 fusion (C6;R34) harboring ROS1 G2032R/L2086F 
            mutation in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Lin JJ, Choudhury NJ, Yoda S, Zhu VW, Johnson TW, Sakhtemani R, 
            Dagogo-Jack I, Digumarthy SR, Lee C, Do A, Peterson J, 
            Prutisto-Chang K, Malik W, Hubbeling HG, Langenbucher A, Schoenfeld 
            AJ, Falcon CJ, Temel JS, Sequist LV, Yeap BY, Lennerz JK, Shaw AT, 
            Lawrence MS, Ou SI, Hata AN, Drilon A, Gainor JF
  TITLE     Spectrum of Mechanisms of Resistance to Crizotinib and Lorlatinib in
            ROS1 Fusion-Positive Lung Cancer.
  JOURNAL   Clin Cancer Res. 2021 May 15;27(10):2899-2909. doi: 
            10.1158/1078-0432.CCR-21-0032. Epub 2021 Mar 8.
  PUBMED    33685866
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/1078-0432.CCR-21-0032"; journalName: "Clin Cancer Res"; 
            date: "2021-05-15- 15"; volume: "27"; issue: "10"; pages: 
            "2899-2909"
COMMENT     Sequence Label: pLenti-CD74-ROS1 G2032R/L2086F
FEATURES             Location/Qualifiers
     source          1..9989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        53..256
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     207..227
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     253..277
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     352..371
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        352..370
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    400..424
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2573..2597)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2629..2652
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2668..2694
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2668..2694
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2701..3267
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2717..3269
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2861..2878)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3088..3107
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3281..3880
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3281..3300)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3777..3797
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3897..4485
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3950..3970)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4368..4379)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4560..4793
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4912..4930)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4998..5102
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5103..5171,5172..5963)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5321..5340)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6134..6722
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6623..6642
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6876..6893
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7133..7154
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7169..7174,7175..7192,7193..7199)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7207..7223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7212..7234
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7231..7247
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7231..7247
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7297..7316
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7508..8141
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8188..8313
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8810..9043
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9228..9272
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9569..9686
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        join(9738..9989,1..52)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
       61 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      121 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      181 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      241 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
      301 ttttgacctc catagaagac accggcggcc gcagatctct cgaggttgat ctaatacgac
      361 tcactatagg gagacccaag ctggctagtt aagctatcaa caagtttgta caaaaaagca
      421 ggctccgcgg ccgccccctt caccatgcac aggaggagaa gcaggagctg tcgggaagat
      481 cagaagccag tcatggatga ccagcgcgac cttatctcca acaatgagca actgcccatg
      541 ctgggccggc gccctggggc cccggagagc aagtgcagcc gcggagccct gtacacaggc
      601 ttttccatcc tggtgactct gctcctcgct ggccaggcca ccaccgccta cttcctgtac
      661 cagcagcagg gccggctgga caaactgaca gtcacctccc agaacctgca gctggagaac
      721 ctgcgcatga agcttcccaa gcctcccaag cctgtgagca agatgcgcat ggccaccccg
      781 ctgctgatgc aggcgctgcc catgggagcc ctgccccagg ggcccatgca gaatgccacc
      841 aagtatggca acatgacaga ggaccatgtg atgcacctgc tccagaatgc tgaccccctg
      901 aaggtgtacc cgccactgaa ggggagcttc ccggagaacc tgagacacct taagaacacc
      961 atggagacca tagactggaa ggtctttgag agctggatgc accattggct cctgtttgaa
     1021 atgagcaggc actccttgga gcaaaagccc actgacgctc caccgaaaga tgatttttgg
     1081 ataccagaaa caagtttcat acttactatt atagttggaa tatttctggt tgttacaatc
     1141 ccactgacct ttgtctggca tagaagatta aagaatcaaa aaagtgccaa ggaaggggtg
     1201 acagtgctta taaacgaaga caaagagttg gctgagctgc gaggtctggc agccggagta
     1261 ggcctggcta atgcctgcta tgcaatacat actcttccaa cccaagagga gattgaaaat
     1321 cttcctgcct tccctcggga aaaactgact ctgcgtctct tgctgggaag tggagccttt
     1381 ggagaagtgt atgaaggaac agcagtggac atcttaggag ttggaagtgg agaaatcaaa
     1441 gtagcagtga agactttgaa gaagggttcc acagaccagg agaagattga attcctgaag
     1501 gaggcacatc tgatgagcaa atttaatcat cccaacattc tgaagcagct tggagtttgt
     1561 ctgctgaatg aaccccaata cattatcctg gaactgatgg agggaagaga ccttcttact
     1621 tatttgcgta aagcccggat ggcaacgttt tatggtcctt tactcacctt ggttgacctt
     1681 gtagacctgt gtgtagatat ttcaaaaggc tgtgtctact tggaacggat gcatttcatt
     1741 cacagggatc tggcagctag aaattgcttt gtttccgtga aagactatac cagtccacgg
     1801 atagtgaaga ttggagactt tggactcgcc agagacatct ataaaaatga ttactataga
     1861 aagagagggg aaggcctgct cccagttcgg tggatggctc cagaaagttt gatggatgga
     1921 atcttcacta ctcaatctga tgtatggtct tttggaattc tgatttggga gattttaact
     1981 cttggtcatc agccttatcc agctcattcc aaccttgatg tgttaaacta tgtgcaaaca
     2041 ggagggagac tggagccacc aagaaattgt cctgatgatc tgtggaattt aatgacccag
     2101 tgctgggctc aagaacccga ccaaagacct acttttcata gaattcagga ccaacttcag
     2161 ttattcagaa attttttctt aaatagcatt tataagtcca gagatgaagc aaacaacagt
     2221 ggagtcataa atgaaagctt tgaaggtgaa gatggcgatg tgatttgttt gaattcagat
     2281 gacattatgc cagttgcttt aatggaaacg aagaaccgag aagggttaaa ctatatggta
     2341 cttgctacag aatgtggcca aggtgaagaa aagtctgagg gtcctctagg ctcccaggaa
     2401 tctgaatctt gtggtctgag gaaagaagag aaggaaccac atgcagacaa agatttctgc
     2461 caagaaaaac aagtggctta ctgcccttct ggcaagcctg aaggcctgaa ctatgcctgt
     2521 ctcactcaca gtggatatgg agatgggtct gattaaaagg gtgggcgcgc cgacccagct
     2581 ttcttgtaca aagtggttta gctagagggc ccgcggttcg aactcgagga ctacaaggac
     2641 gacgatgaca agtcggccgc tggaggatac ccctacgacg tgcccgacta cgcctagaat
     2701 tccgcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     2761 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     2821 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     2881 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     2941 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     3001 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     3061 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     3121 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     3181 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     3241 gttttccttt gaaaaacacg atgataatat ggccacaacc atgaccgagt acaagcccac
     3301 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     3361 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     3421 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     3481 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     3541 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     3601 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     3661 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     3721 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     3781 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     3841 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga tgcatcgatg gatcctaatc
     3901 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     3961 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     4021 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4081 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4141 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     4201 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     4261 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     4321 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     4381 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     4441 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag
     4501 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     4561 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     4621 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     4681 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     4741 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     4801 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     4861 gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga
     4921 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     4981 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5041 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5101 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5161 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5221 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5281 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5341 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5401 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5461 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5521 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5581 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5641 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5701 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5761 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5821 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5881 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5941 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6001 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6061 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6121 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6181 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6241 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6301 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6361 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6421 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6481 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6541 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6601 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6661 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6721 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6781 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6841 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     6901 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca
     6961 agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7021 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     7081 cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     7141 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     7201 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt
     7261 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7321 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
     7381 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
     7441 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
     7501 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
     7561 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     7621 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     7681 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     7741 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     7801 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     7861 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     7921 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     7981 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8041 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8101 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     8161 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     8221 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     8281 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     8341 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     8401 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     8461 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     8521 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     8581 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     8641 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     8701 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     8761 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     8821 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     8881 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     8941 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     9001 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     9061 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     9121 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     9181 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     9241 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     9301 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     9361 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     9421 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     9481 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgccgaatta
     9541 attcacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     9601 gtgcagggaa aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     9661 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt
     9721 ggactagtgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     9781 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     9841 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     9901 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     9961 tggcattatg cccagtacat gaccttatg
//
