LOCUS       sequence-360530-        9989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/L2086F 
            mutation in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Lin JJ, Choudhury NJ, Yoda S, Zhu VW, Johnson TW, Sakhtemani R, 
            Dagogo-Jack I, Digumarthy SR, Lee C, Do A, Peterson J, 
            Prutisto-Chang K, Malik W, Hubbeling HG, Langenbucher A, Schoenfeld 
            AJ, Falcon CJ, Temel JS, Sequist LV, Yeap BY, Lennerz JK, Shaw AT, 
            Lawrence MS, Ou SI, Hata AN, Drilon A, Gainor JF
  TITLE     Spectrum of Mechanisms of Resistance to Crizotinib and Lorlatinib in
            ROS1 Fusion-Positive Lung Cancer.
  JOURNAL   Clin Cancer Res. 2021 May 15;27(10):2899-2909. doi: 
            10.1158/1078-0432.CCR-21-0032. Epub 2021 Mar 8.
  PUBMED    33685866
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/1078-0432.CCR-21-0032"; journalName: "Clin Cancer Res"; 
            date: "2021-05-15- 15"; volume: "27"; issue: "10"; pages: 
            "2899-2909"
COMMENT     Sequence Label: pLenti-CD74-ROS1 S1986F/L2086F
FEATURES             Location/Qualifiers
     source          1..9989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        91..294
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     245..265
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     291..315
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     390..409
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        390..408
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    438..462
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2611..2635)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2667..2690
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2706..2732
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2706..2732
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2739..3305
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2755..3307
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2899..2916)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3126..3145
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3319..3918
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3319..3338)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3815..3835
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3935..4523
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3988..4008)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4406..4417)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4598..4831
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4950..4968)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5036..5140
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5141..5209,5210..6001)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5359..5378)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6172..6760
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6661..6680
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6914..6931
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7171..7192
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7207..7212,7213..7230,7231..7237)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7245..7261
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7250..7272
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7269..7285
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7269..7285
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7335..7354
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7546..8179
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8226..8351
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8848..9081
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9266..9310
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9607..9724
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        join(9776..9989,1..90)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
       61 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      121 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      181 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      241 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      301 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      361 cggcggccgc agatctctcg aggttgatct aatacgactc actataggga gacccaagct
      421 ggctagttaa gctatcaaca agtttgtaca aaaaagcagg ctccgcggcc gcccccttca
      481 ccatgcacag gaggagaagc aggagctgtc gggaagatca gaagccagtc atggatgacc
      541 agcgcgacct tatctccaac aatgagcaac tgcccatgct gggccggcgc cctggggccc
      601 cggagagcaa gtgcagccgc ggagccctgt acacaggctt ttccatcctg gtgactctgc
      661 tcctcgctgg ccaggccacc accgcctact tcctgtacca gcagcagggc cggctggaca
      721 aactgacagt cacctcccag aacctgcagc tggagaacct gcgcatgaag cttcccaagc
      781 ctcccaagcc tgtgagcaag atgcgcatgg ccaccccgct gctgatgcag gcgctgccca
      841 tgggagccct gccccagggg cccatgcaga atgccaccaa gtatggcaac atgacagagg
      901 accatgtgat gcacctgctc cagaatgctg accccctgaa ggtgtacccg ccactgaagg
      961 ggagcttccc ggagaacctg agacacctta agaacaccat ggagaccata gactggaagg
     1021 tctttgagag ctggatgcac cattggctcc tgtttgaaat gagcaggcac tccttggagc
     1081 aaaagcccac tgacgctcca ccgaaagatg atttttggat accagaaaca agtttcatac
     1141 ttactattat agttggaata tttctggttg ttacaatccc actgaccttt gtctggcata
     1201 gaagattaaa gaatcaaaaa agtgccaagg aaggggtgac agtgcttata aacgaagaca
     1261 aagagttggc tgagctgcga ggtctggcag ccggagtagg cctggctaat gcctgctatg
     1321 caatacatac tcttccaacc caagaggaga ttgaaaatct tcctgccttc cctcgggaaa
     1381 aactgactct gcgtctcttg ctgggaagtg gagcctttgg agaagtgtat gaaggaacag
     1441 cagtggacat cttaggagtt ggaagtggag aaatcaaagt agcagtgaag actttgaaga
     1501 agggtttcac agaccaggag aagattgaat tcctgaagga ggcacatctg atgagcaaat
     1561 ttaatcatcc caacattctg aagcagcttg gagtttgtct gctgaatgaa ccccaataca
     1621 ttatcctgga actgatggag ggaggagacc ttcttactta tttgcgtaaa gcccggatgg
     1681 caacgtttta tggtccttta ctcaccttgg ttgaccttgt agacctgtgt gtagatattt
     1741 caaaaggctg tgtctacttg gaacggatgc atttcattca cagggatctg gcagctagaa
     1801 attgctttgt ttccgtgaaa gactatacca gtccacggat agtgaagatt ggagactttg
     1861 gactcgccag agacatctat aaaaatgatt actatagaaa gagaggggaa ggcctgctcc
     1921 cagttcggtg gatggctcca gaaagtttga tggatggaat cttcactact caatctgatg
     1981 tatggtcttt tggaattctg atttgggaga ttttaactct tggtcatcag ccttatccag
     2041 ctcattccaa ccttgatgtg ttaaactatg tgcaaacagg agggagactg gagccaccaa
     2101 gaaattgtcc tgatgatctg tggaatttaa tgacccagtg ctgggctcaa gaacccgacc
     2161 aaagacctac ttttcataga attcaggacc aacttcagtt attcagaaat tttttcttaa
     2221 atagcattta taagtccaga gatgaagcaa acaacagtgg agtcataaat gaaagctttg
     2281 aaggtgaaga tggcgatgtg atttgtttga attcagatga cattatgcca gttgctttaa
     2341 tggaaacgaa gaaccgagaa gggttaaact atatggtact tgctacagaa tgtggccaag
     2401 gtgaagaaaa gtctgagggt cctctaggct cccaggaatc tgaatcttgt ggtctgagga
     2461 aagaagagaa ggaaccacat gcagacaaag atttctgcca agaaaaacaa gtggcttact
     2521 gcccttctgg caagcctgaa ggcctgaact atgcctgtct cactcacagt ggatatggag
     2581 atgggtctga ttaaaagggt gggcgcgccg acccagcttt cttgtacaaa gtggtttagc
     2641 tagagggccc gcggttcgaa ctcgaggact acaaggacga cgatgacaag tcggccgctg
     2701 gaggataccc ctacgacgtg cccgactacg cctagaattc cgcccccccc cctaacgtta
     2761 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     2821 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     2881 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     2941 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     3001 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     3061 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     3121 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     3181 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     3241 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     3301 gataatatgg ccacaaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     3361 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     3421 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     3481 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     3541 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     3601 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     3661 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     3721 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     3781 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     3841 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     3901 cgcaagcccg gtgcctgatg catcgatgga tcctaatcaa cctctggatt acaaaatttg
     3961 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     4021 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     4081 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     4141 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     4201 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     4261 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     4321 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     4381 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     4441 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     4501 ctccctttgg gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct
     4561 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     4621 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     4681 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4741 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4801 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     4861 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa
     4921 agggctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     4981 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5041 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5101 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5161 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5221 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5281 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5341 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     5401 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5461 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5521 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5581 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5641 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     5701 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5761 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5821 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     5881 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5941 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6001 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6061 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6121 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6181 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6241 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6301 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6361 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6421 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6481 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6541 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6601 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6661 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6721 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6781 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     6841 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     6901 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     6961 gcctctcccc gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt
     7021 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7081 ggcttttttg gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga
     7141 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     7201 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     7261 atttcacaca ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc
     7321 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     7381 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
     7441 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
     7501 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc
     7561 actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     7621 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     7681 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     7741 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     7801 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     7861 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     7921 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     7981 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     8041 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     8101 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     8161 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     8221 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     8281 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     8341 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     8401 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     8461 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     8521 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     8581 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     8641 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct
     8701 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     8761 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     8821 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     8881 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     8941 tggtatagtg cagcagcaga acaatttgct gatggctatt gaggcgcaac agcatctgtt
     9001 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     9061 cctaaaggat caacagctcc tggggatttt gggttgctct ggaaaactca tttgcaccac
     9121 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     9181 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     9241 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     9301 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     9361 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     9421 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     9481 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     9541 tcgattagtg aacggatctc gacggtatcg ccgaattaat tcacaaatgg cagtattcat
     9601 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcagggaaaa gaatagtaga
     9661 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     9721 ttttcgggtt tattacaggg acagcagaga tccagtttgg actagtggag ttccgcgtta
     9781 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     9841 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     9901 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     9961 cgccccctat tgacgtcaat gacggtaaa
//
