LOCUS       sequence-360531-        9989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/L2000V 
            mutation in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Lin JJ, Choudhury NJ, Yoda S, Zhu VW, Johnson TW, Sakhtemani R, 
            Dagogo-Jack I, Digumarthy SR, Lee C, Do A, Peterson J, 
            Prutisto-Chang K, Malik W, Hubbeling HG, Langenbucher A, Schoenfeld 
            AJ, Falcon CJ, Temel JS, Sequist LV, Yeap BY, Lennerz JK, Shaw AT, 
            Lawrence MS, Ou SI, Hata AN, Drilon A, Gainor JF
  TITLE     Spectrum of Mechanisms of Resistance to Crizotinib and Lorlatinib in
            ROS1 Fusion-Positive Lung Cancer.
  JOURNAL   Clin Cancer Res. 2021 May 15;27(10):2899-2909. doi: 
            10.1158/1078-0432.CCR-21-0032. Epub 2021 Mar 8.
  PUBMED    33685866
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/1078-0432.CCR-21-0032"; journalName: "Clin Cancer Res"; 
            date: "2021-05-15- 15"; volume: "27"; issue: "10"; pages: 
            "2899-2909"
COMMENT     Sequence Label: pLenti-CD74-ROS1 S1986F/L2000V
FEATURES             Location/Qualifiers
     source          1..9989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        76..279
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     230..250
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     276..300
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     375..394
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        375..393
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    423..447
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2596..2620)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2652..2675
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2691..2717
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2691..2717
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2724..3290
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2740..3292
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2884..2901)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3111..3130
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3304..3903
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3304..3323)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3800..3820
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3920..4508
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3973..3993)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4391..4402)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4583..4816
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4935..4953)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5021..5125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5126..5194,5195..5986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5344..5363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6157..6745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6646..6665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6899..6916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7156..7177
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7192..7197,7198..7215,7216..7222)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7230..7246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7235..7257
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7254..7270
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7254..7270
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7320..7339
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7531..8164
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8211..8336
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8833..9066
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9251..9295
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9592..9709
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        join(9761..9989,1..75)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
       61 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      121 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      181 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      241 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
      301 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggcg gccgcagatc
      361 tctcgaggtt gatctaatac gactcactat agggagaccc aagctggcta gttaagctat
      421 caacaagttt gtacaaaaaa gcaggctccg cggccgcccc cttcaccatg cacaggagga
      481 gaagcaggag ctgtcgggaa gatcagaagc cagtcatgga tgaccagcgc gaccttatct
      541 ccaacaatga gcaactgccc atgctgggcc ggcgccctgg ggccccggag agcaagtgca
      601 gccgcggagc cctgtacaca ggcttttcca tcctggtgac tctgctcctc gctggccagg
      661 ccaccaccgc ctacttcctg taccagcagc agggccggct ggacaaactg acagtcacct
      721 cccagaacct gcagctggag aacctgcgca tgaagcttcc caagcctccc aagcctgtga
      781 gcaagatgcg catggccacc ccgctgctga tgcaggcgct gcccatggga gccctgcccc
      841 aggggcccat gcagaatgcc accaagtatg gcaacatgac agaggaccat gtgatgcacc
      901 tgctccagaa tgctgacccc ctgaaggtgt acccgccact gaaggggagc ttcccggaga
      961 acctgagaca ccttaagaac accatggaga ccatagactg gaaggtcttt gagagctgga
     1021 tgcaccattg gctcctgttt gaaatgagca ggcactcctt ggagcaaaag cccactgacg
     1081 ctccaccgaa agatgatttt tggataccag aaacaagttt catacttact attatagttg
     1141 gaatatttct ggttgttaca atcccactga cctttgtctg gcatagaaga ttaaagaatc
     1201 aaaaaagtgc caaggaaggg gtgacagtgc ttataaacga agacaaagag ttggctgagc
     1261 tgcgaggtct ggcagccgga gtaggcctgg ctaatgcctg ctatgcaata catactcttc
     1321 caacccaaga ggagattgaa aatcttcctg ccttccctcg ggaaaaactg actctgcgtc
     1381 tcttgctggg aagtggagcc tttggagaag tgtatgaagg aacagcagtg gacatcttag
     1441 gagttggaag tggagaaatc aaagtagcag tgaagacttt gaagaagggt ttcacagacc
     1501 aggagaagat tgaattcctg aaggaggcac atgtgatgag caaatttaat catcccaaca
     1561 ttctgaagca gcttggagtt tgtctgctga atgaacccca atacattatc ctggaactga
     1621 tggagggagg agaccttctt acttatttgc gtaaagcccg gatggcaacg ttttatggtc
     1681 ctttactcac cttggttgac cttgtagacc tgtgtgtaga tatttcaaaa ggctgtgtct
     1741 acttggaacg gatgcatttc attcacaggg atctggcagc tagaaattgc cttgtttccg
     1801 tgaaagacta taccagtcca cggatagtga agattggaga ctttggactc gccagagaca
     1861 tctataaaaa tgattactat agaaagagag gggaaggcct gctcccagtt cggtggatgg
     1921 ctccagaaag tttgatggat ggaatcttca ctactcaatc tgatgtatgg tcttttggaa
     1981 ttctgatttg ggagatttta actcttggtc atcagcctta tccagctcat tccaaccttg
     2041 atgtgttaaa ctatgtgcaa acaggaggga gactggagcc accaagaaat tgtcctgatg
     2101 atctgtggaa tttaatgacc cagtgctggg ctcaagaacc cgaccaaaga cctacttttc
     2161 atagaattca ggaccaactt cagttattca gaaatttttt cttaaatagc atttataagt
     2221 ccagagatga agcaaacaac agtggagtca taaatgaaag ctttgaaggt gaagatggcg
     2281 atgtgatttg tttgaattca gatgacatta tgccagttgc tttaatggaa acgaagaacc
     2341 gagaagggtt aaactatatg gtacttgcta cagaatgtgg ccaaggtgaa gaaaagtctg
     2401 agggtcctct aggctcccag gaatctgaat cttgtggtct gaggaaagaa gagaaggaac
     2461 cacatgcaga caaagatttc tgccaagaaa aacaagtggc ttactgccct tctggcaagc
     2521 ctgaaggcct gaactatgcc tgtctcactc acagtggata tggagatggg tctgattaaa
     2581 agggtgggcg cgccgaccca gctttcttgt acaaagtggt ttagctagag ggcccgcggt
     2641 tcgaactcga ggactacaag gacgacgatg acaagtcggc cgctggagga tacccctacg
     2701 acgtgcccga ctacgcctag aattccgccc ccccccctaa cgttactggc cgaagccgct
     2761 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     2821 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     2881 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     2941 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     3001 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     3061 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     3121 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     3181 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     3241 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca
     3301 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     3361 gtacgcaccc tcgccgcagc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     3421 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     3481 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     3541 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     3601 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     3661 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     3721 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     3781 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     3841 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     3901 tgatgcatcg atggatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3961 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4021 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4081 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4141 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     4201 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4261 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     4321 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4381 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4441 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4501 ctccccgcct gagatccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     4561 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     4621 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     4681 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     4741 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     4801 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     4861 aagaaatgaa tatcagagag tgagaggccc gggttaatta aggaaagggc tagatcattc
     4921 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     4981 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     5041 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     5101 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     5161 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     5221 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     5281 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     5341 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     5401 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5461 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5521 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5581 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5641 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5701 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5761 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5821 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5881 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5941 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6001 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6061 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6121 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6181 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6241 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6301 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6361 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6421 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6481 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6541 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6601 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6661 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6721 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6781 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6841 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6901 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     6961 ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag
     7021 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7081 ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag
     7141 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     7201 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     7261 cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg
     7321 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
     7381 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
     7441 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
     7501 ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac
     7561 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
     7621 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     7681 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     7741 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     7801 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
     7861 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     7921 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
     7981 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8041 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8101 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8161 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     8221 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     8281 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     8341 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     8401 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     8461 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     8521 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     8581 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     8641 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg
     8701 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     8761 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     8821 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     8881 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     8941 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     9001 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     9061 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     9121 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     9181 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     9241 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     9301 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     9361 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     9421 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     9481 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     9541 atctcgacgg tatcgccgaa ttaattcaca aatggcagta ttcatccaca attttaaaag
     9601 aaaagggggg attggggggt acagtgcagg gaaaagaata gtagacataa tagcaacaga
     9661 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     9721 cagggacagc agagatccag tttggactag tggagttccg cgttacataa cttacggtaa
     9781 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     9841 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     9901 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     9961 tcaatgacgg taaatggccc gcctggcat
//
