LOCUS       sequence-360533-        9989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CD74-ROS1 fusion (C6;R34) harboring ROS1 L2086F mutation in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Lin JJ, Choudhury NJ, Yoda S, Zhu VW, Johnson TW, Sakhtemani R, 
            Dagogo-Jack I, Digumarthy SR, Lee C, Do A, Peterson J, 
            Prutisto-Chang K, Malik W, Hubbeling HG, Langenbucher A, Schoenfeld 
            AJ, Falcon CJ, Temel JS, Sequist LV, Yeap BY, Lennerz JK, Shaw AT, 
            Lawrence MS, Ou SI, Hata AN, Drilon A, Gainor JF
  TITLE     Spectrum of Mechanisms of Resistance to Crizotinib and Lorlatinib in
            ROS1 Fusion-Positive Lung Cancer.
  JOURNAL   Clin Cancer Res. 2021 May 15;27(10):2899-2909. doi: 
            10.1158/1078-0432.CCR-21-0032. Epub 2021 Mar 8.
  PUBMED    33685866
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/1078-0432.CCR-21-0032"; journalName: "Clin Cancer Res"; 
            date: "2021-05-15- 15"; volume: "27"; issue: "10"; pages: 
            "2899-2909"
COMMENT     Sequence Label: pLenti-CD74-ROS1 L2086F
FEATURES             Location/Qualifiers
     source          1..9989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        142..345
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     296..316
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     342..366
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     441..460
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        441..459
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    489..513
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2662..2686)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2718..2741
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2757..2783
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2757..2783
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2790..3356
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2806..3358
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2950..2967)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3177..3196
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3370..3969
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3370..3389)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3866..3886
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3986..4574
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4039..4059)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4457..4468)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4649..4882
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5001..5019)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5087..5191
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5192..5260,5261..6052)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5410..5429)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6223..6811
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6712..6731
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6965..6982
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7222..7243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7258..7263,7264..7281,7282..7288)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7296..7312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7301..7323
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7320..7336
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7320..7336
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7386..7405
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7597..8230
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8277..8402
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8899..9132
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9317..9361
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9658..9775
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        join(9827..9989,1..141)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
       61 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      121 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      181 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      241 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      301 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
      361 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccggcggccg
      421 cagatctctc gaggttgatc taatacgact cactataggg agacccaagc tggctagtta
      481 agctatcaac aagtttgtac aaaaaagcag gctccgcggc cgcccccttc accatgcaca
      541 ggaggagaag caggagctgt cgggaagatc agaagccagt catggatgac cagcgcgacc
      601 ttatctccaa caatgagcaa ctgcccatgc tgggccggcg ccctggggcc ccggagagca
      661 agtgcagccg cggagccctg tacacaggct tttccatcct ggtgactctg ctcctcgctg
      721 gccaggccac caccgcctac ttcctgtacc agcagcaggg ccggctggac aaactgacag
      781 tcacctccca gaacctgcag ctggagaacc tgcgcatgaa gcttcccaag cctcccaagc
      841 ctgtgagcaa gatgcgcatg gccaccccgc tgctgatgca ggcgctgccc atgggagccc
      901 tgccccaggg gcccatgcag aatgccacca agtatggcaa catgacagag gaccatgtga
      961 tgcacctgct ccagaatgct gaccccctga aggtgtaccc gccactgaag gggagcttcc
     1021 cggagaacct gagacacctt aagaacacca tggagaccat agactggaag gtctttgaga
     1081 gctggatgca ccattggctc ctgtttgaaa tgagcaggca ctccttggag caaaagccca
     1141 ctgacgctcc accgaaagat gatttttgga taccagaaac aagtttcata cttactatta
     1201 tagttggaat atttctggtt gttacaatcc cactgacctt tgtctggcat agaagattaa
     1261 agaatcaaaa aagtgccaag gaaggggtga cagtgcttat aaacgaagac aaagagttgg
     1321 ctgagctgcg aggtctggca gccggagtag gcctggctaa tgcctgctat gcaatacata
     1381 ctcttccaac ccaagaggag attgaaaatc ttcctgcctt ccctcgggaa aaactgactc
     1441 tgcgtctctt gctgggaagt ggagcctttg gagaagtgta tgaaggaaca gcagtggaca
     1501 tcttaggagt tggaagtgga gaaatcaaag tagcagtgaa gactttgaag aagggttcca
     1561 cagaccagga gaagattgaa ttcctgaagg aggcacatct gatgagcaaa tttaatcatc
     1621 ccaacattct gaagcagctt ggagtttgtc tgctgaatga accccaatac attatcctgg
     1681 aactgatgga gggaggagac cttcttactt atttgcgtaa agcccggatg gcaacgtttt
     1741 atggtccttt actcaccttg gttgaccttg tagacctgtg tgtagatatt tcaaaaggct
     1801 gtgtctactt ggaacggatg catttcattc acagggatct ggcagctaga aattgctttg
     1861 tttccgtgaa agactatacc agtccacgga tagtgaagat tggagacttt ggactcgcca
     1921 gagacatcta taaaaatgat tactatagaa agagagggga aggcctgctc ccagttcggt
     1981 ggatggctcc agaaagtttg atggatggaa tcttcactac tcaatctgat gtatggtctt
     2041 ttggaattct gatttgggag attttaactc ttggtcatca gccttatcca gctcattcca
     2101 accttgatgt gttaaactat gtgcaaacag gagggagact ggagccacca agaaattgtc
     2161 ctgatgatct gtggaattta atgacccagt gctgggctca agaacccgac caaagaccta
     2221 cttttcatag aattcaggac caacttcagt tattcagaaa ttttttctta aatagcattt
     2281 ataagtccag agatgaagca aacaacagtg gagtcataaa tgaaagcttt gaaggtgaag
     2341 atggcgatgt gatttgtttg aattcagatg acattatgcc agttgcttta atggaaacga
     2401 agaaccgaga agggttaaac tatatggtac ttgctacaga atgtggccaa ggtgaagaaa
     2461 agtctgaggg tcctctaggc tcccaggaat ctgaatcttg tggtctgagg aaagaagaga
     2521 aggaaccaca tgcagacaaa gatttctgcc aagaaaaaca agtggcttac tgcccttctg
     2581 gcaagcctga aggcctgaac tatgcctgtc tcactcacag tggatatgga gatgggtctg
     2641 attaaaaggg tgggcgcgcc gacccagctt tcttgtacaa agtggtttag ctagagggcc
     2701 cgcggttcga actcgaggac tacaaggacg acgatgacaa gtcggccgct ggaggatacc
     2761 cctacgacgt gcccgactac gcctagaatt ccgccccccc ccctaacgtt actggccgaa
     2821 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     2881 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     2941 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     3001 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     3061 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     3121 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     3181 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     3241 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     3301 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
     3361 gccacaacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     3421 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     3481 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     3541 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     3601 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     3661 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     3721 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     3781 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     3841 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     3901 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     3961 ggtgcctgat gcatcgatgg atcctaatca acctctggat tacaaaattt gtgaaagatt
     4021 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4081 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4141 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4201 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4261 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4321 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4381 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4441 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4501 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     4561 ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt acaaggcagc tgtagatctt
     4621 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     4681 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     4741 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     4801 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     4861 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     4921 cttgcaaaga aatgaatatc agagagtgag aggcccgggt taattaagga aagggctaga
     4981 tcattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     5041 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5101 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5161 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5221 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5281 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5341 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5401 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     5461 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5521 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5581 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5641 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5701 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     5761 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5821 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5881 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5941 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6001 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6061 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6121 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6181 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6241 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6301 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6361 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6421 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6481 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6541 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6601 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6661 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6721 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6781 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6841 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6901 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     6961 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     7021 cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct gactaatttt ttttatttat
     7081 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7141 ggaggcctag gcttttgcaa aaagctcccc gtggcacgac aggtttcccg actggaaagc
     7201 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     7261 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     7321 aggaaacagc tatgacatga ttacgaattt cacaaataaa gcattttttt cactgcattc
     7381 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
     7441 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
     7501 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
     7561 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
     7621 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     7681 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     7741 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     7801 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     7861 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     7921 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     7981 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     8041 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8101 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8161 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8221 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     8281 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     8341 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     8401 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     8461 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     8521 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     8581 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     8641 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     8701 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
     8761 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     8821 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     8881 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     8941 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     9001 gcagcagcag aacaatttgc tgatggctat tgaggcgcaa cagcatctgt tgcaactcac
     9061 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     9121 tcaacagctc ctggggattt tgggttgctc tggaaaactc atttgcacca ctgctgtgcc
     9181 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     9241 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     9301 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     9361 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     9421 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     9481 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     9541 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     9601 gaacggatct cgacggtatc gccgaattaa ttcacaaatg gcagtattca tccacaattt
     9661 taaaagaaaa ggggggattg gggggtacag tgcagggaaa agaatagtag acataatagc
     9721 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     9781 ttattacagg gacagcagag atccagtttg gactagtgga gttccgcgtt acataactta
     9841 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     9901 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     9961 tacggtaaac tgcccacttg gcagtacat
//
