LOCUS       sequence-360538-        9164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral plasmid encoding pH-Countdown and puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Seidel ZP, Wang JCK, Riegler J, York AG, Ingaramo M
  TITLE     Relaxation Sensors
  JOURNAL   (2021), doi: 10.5281/zenodo.5810930
REFERENCE   2  (bases 1 to 9164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "(2021), 
            doi: 10.5281/zenodo.5810930"
COMMENT     Sequence Label: pCDH-EF1-pHCountdown
FEATURES             Location/Qualifiers
     source          1..9164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    1932..2156
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2166..2765)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2249..2269)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2746..2765
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2790..3289)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2838..2857)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3231..3254
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        3303..4481
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3534..4472
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4429..4449
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4500..4509
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5243..5831
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5296..5316)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5714..5725)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5905..6138
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6210..6331
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6247..6266)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6301..6320
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6371..6506
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6539..6555)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6539..6555)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6552..6574)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6563..6579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6587..6593,6594..6611,6612..6617))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6632..6653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6770..6787)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6941..7529)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7700..8491,8492..8560))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8323..8342
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8561..8665)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8733..8751
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8789..8811)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8911..8930
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9124..9146
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9138..9155
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9139..9155
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatctcga gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc
     1981 ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca
     2041 atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt
     2101 caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcgc
     2161 tcgagtcagg caccgggctt gcgggtcatg caccaggtgc gcggtccttc gggcacctcg
     2221 acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag
     2281 gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg gagcacgacg
     2341 gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc caggaaccac
     2401 gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg cgcggccagc
     2461 cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg
     2521 acgctctccg gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac ccacaccttg
     2581 ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac ccgctcgatg
     2641 tggcggtccg gatcgacggt gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg
     2701 gtgcgtacgg ccctggggac gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg
     2761 gtcatccatg gtaagcttgg gctgcaggtc gaaaggcccg gagatgagga agaggagaac
     2821 agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg ggcctccgga ggaccttcgg
     2881 gcgcccgccc cgcccctgag cccgcccctg agcccgcccc cggacccacc ccttcccagc
     2941 ctctgagccc agaaagcgaa ggagcaaagc tgctattggc cgctgcccca aaggcctacc
     3001 cgcttccatt gctcagcggt gctgtccatc tgcacgagac tagtgagacg tgctacttcc
     3061 atttgtcacg tcctgcacga cgcgagctgc ggggcggggg ggaacttcct gactagggga
     3121 ggagtagaag gtggcgcgaa ggggccacca aagaacggag ccggttggcg cctaccggtg
     3181 gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc gccaagtgcc cagcggggct
     3241 gctaaagcgc atgctccaga ctgccttggg aaaagcgcct cccctacccg gtagaagcta
     3301 gcggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgt
     3361 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     3421 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     3481 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     3541 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     3601 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     3661 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     3721 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     3781 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     3841 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     3901 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     3961 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     4021 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     4081 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     4141 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     4201 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     4261 attagttctc gaccttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     4321 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     4381 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     4441 cagtggttca aagttttttt cttccatttc aggtgtcgtg atctagagct agcgaattcg
     4501 ccgccatggt gagcaagggc gaggagaaca acatggccgt gattaaacca gacatgaaga
     4561 tcaagctgcg tatggaaggc tctgtaaatg gacaccgttt cagaattgaa ggagttggcc
     4621 ttgggaagcc tcttgaggga aaacagagta tggaccttaa agtcaaagaa ggcggacctc
     4681 tgcctttcgc ctatgacatc ttgacaatgg cgttctgtta cggtaacagg gtattcgcca
     4741 aatacccaga aaatatagta gactatttca agcagtcgtt tcctgagggc tactcttggg
     4801 aacgacaaat gatttacgaa gacgggggca tttgtgtcgc gacaaacgac ataaccctgg
     4861 atggtgactg tatgatctcc gaaattcgat ttaagggtgt gaactttcct gccaatggtc
     4921 cagttttcca gaagaggact gtgaaatggg agctatccca cgagaaattg tatgctcgtg
     4981 atggactgct gtactctgat ggtaactacg ctctgtcgct tgaaggaggt ggccattacc
     5041 gatgtgacaa taaaactact tataaagcta agaaggttgt ccagttgcca gactatcact
     5101 gggtgaccca cagtattgtg attaaaagcc acgacaaaga ttacagtaat gttaatctgc
     5161 atgagcatgc cgaagcacat tctgagctgc cgaggcaggc catggacgag ctgtacaagt
     5221 aaggatccgc ggccgcgtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     5281 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     5341 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     5401 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     5461 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     5521 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     5581 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     5641 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     5701 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     5761 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     5821 cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     5881 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc
     5941 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6001 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6061 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6121 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     6181 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     6241 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6301 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     6361 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6421 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6481 gtagtgagga ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt
     6541 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6601 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6661 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6721 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6781 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6841 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6901 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6961 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7021 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7081 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7141 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7201 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7261 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7321 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7381 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7441 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7501 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7561 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7621 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7681 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7741 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7801 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7861 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7921 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7981 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8041 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8101 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8161 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8221 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8281 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8341 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8401 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8461 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8521 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8581 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8641 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     8701 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     8761 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     8821 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     8881 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     8941 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     9001 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     9061 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     9121 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
