LOCUS       sequence-360552-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes glutathione S-transferase linked to Q11 self-assembling 
            peptide via a GS-linker sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Shanbhag BK, Liu C, Pradeep GC, Younas T, Hu KKY, Fulcher AJ, Struwe
            WB, Steer D, Dumsday G, Harper IS, Kukura P, Haritos VS, He L
  TITLE     Custom Design of Protein Particles as Multifunctional Biomaterials
  JOURNAL   Adv. Funct. Mater. 2022, 32, 2108039
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Adv. Funct.
            Mater. 2022, 32, 2108039"
COMMENT     Sequence Label: pET28a-GST-Q11
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             27..677
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="SPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKF
                     ELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYG
                     VSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLY
                     MDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
ORIGIN
        1 ccatgggcca tcatcatcat catcatagcc cgatcctggg ttactggaaa atcaagggcc
       61 tggtgcaacc gacccgcctg ctgctggaat acctggagga aaaatacgag gaacacctgt
      121 atgagcgtga cgaaggcgat aagtggcgta acaagaaatt cgagctgggt ctggaatttc
      181 cgaacctgcc gtactatatt gacggcgatg tgaaactgac ccagagcatg gcgatcattc
      241 gttacatcgc ggacaaacac aacatgctgg gtggctgccc gaaggagcgt gcggaaatta
      301 gcatgctgga gggcgcggtg ctggatattc gttacggtgt tagccgtatc gcgtatagca
      361 aagacttcga aaccctgaag gtggattttc tgagcaaact gccggagatg ctgaagatgt
      421 tcgaggaccg tctgtgccac aaaacctatc tgaacggtga ccacgttacc cacccggatt
      481 ttatgctgta cgacgcgctg gatgtggttc tgtatatgga cccgatgtgc ctggatgcgt
      541 tcccgaagct ggtttgcttt aagaaacgta tcgaggcgat tccgcaaatc gacaagtacc
      601 tgaaaagcag caagtatatt gcgtggccgc tgcaaggttg gcaagcgacc tttggtggcg
      661 gtgatcaccc gccgaagggt ggcggtggta gcggcggcgg cggcagccaa cagaaatttc
      721 agtttcagtt tgaacagcag taataactcg ag
//
