LOCUS       sequence-360553-        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes green flourescent protein linked to RNA binding peptide (on 
            N terminal end ) and Z domain fragment (variant 1) of Staphylococcal
            protein A (on C-terminal) via GS-linker .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Younas T, Vidallon MLP, Tabor RF, He L
  TITLE     Open-Closed Structure of Light-Responsive Protein LOV2 Regulates Its
            Molecular Interaction with a Binding Partner.
  JOURNAL   J Phys Chem Lett. 2020 Oct 15;11(20):8647-8653. doi: 
            10.1021/acs.jpclett.0c02252. Epub 2020 Sep 29.
  PUBMED    32945680
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jpclett.0c02252"; journalName: "J Phys Chem Lett"; 
            date: "2020-10-15- 15"; volume: "11"; issue: "20"; pages: 
            "8647-8653"
COMMENT     Sequence Label: pET28a-RBP-GFP(S45C)-ZdK1
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..38
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             51..71
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             144..848
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFI
                     CTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANF
                     KIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELY"
ORIGIN
        1 ccatggcaca tcaccatcat catcaccatc accaccacgg cggcggcagc gagaacctgt
       61 atttccaagg cagctacggt cgcaagaagc gtcgtcagcg tcgtcgtccg ccgcaaggtg
      121 gcggtagcgg cggtggcagc tgcaaaggtg aagagctgtt caccggtgtt gtgccgattc
      181 tggttgagct ggatggtgac gttaacggcc acaaattcag cgttcgtggc gagggtgaag
      241 gcgatgcgac caacggcaag ctgaccctga agttcatctg caccaccggt aaactgccgg
      301 ttccgtggcc gaccctggtg accaccctga cctatggtgt gcaatgcttc gcgcgttatc
      361 cggatcacat gaagcagcac gacttcttta agagcgcgat gccggaaggt tacgttcagg
      421 agcgtaccat cagcttcaag gatgacggca cctacaagac ccgtgcggaa gtgaagttcg
      481 agggtgatac cctggttaac cgtatcgagc tgaaaggcat cgatttcaaa gaggacggta
      541 acattctggg tcacaaactg gagtacaact tcaacagcca caacgtgtac attaccgcgg
      601 ataaacagaa gaacggtatc aaagcgaact ttaagatccg tcacaacgtt gaagatggca
      661 gcgtgcagct ggcggaccac taccaacaga acaccccgat cggcgacggt ccggttctgc
      721 tgccggacaa ccactacctg agcacccaga gcgttctgag caaagacccg aacgaaaagc
      781 gtgaccacat ggtgctgctg gagttcgtga ccgcggcggg tatcacccac ggcatggatg
      841 agctgtacgg tggtggcagc ggcggtggta gcgtggacaa caagttcaac aaggagaaaa
      901 cccgtgcggg tgcggagatt cacagcctgc cgaacctgaa cgtggagcaa aagtttgcgt
      961 tcatcgtgag cctgttcgat gacccgagcc aaagcgcgaa cctgctggcg gaagcgaaga
     1021 aactgaacga tgcgcaagcg ccgaagtaac tcgag
//
