LOCUS       sequence-360558-        4266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC40A1_STOP
FEATURES             Location/Qualifiers
     source          1..4266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1823..1922)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1939..1958)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1940..1958)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1963..1979)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1963..1979)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2081..2100)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2092..2901
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2159..2178)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3048..3636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3537..3556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3790..3807
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3966..3993)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4085..4171)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4220..4242
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4234..4251
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4235..4251
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgac cagggcaggc
      121 gaccacaata ggcagagagg atgctgtgga tccctggcag attacctgac atctgccaag
      181 tttctgctgt atctgggaca cagcctgtcc acctggggcg acagaatgtg gcacttcgcc
      241 gtgtccgtgt ttctggtgga gctgtacggc aactctctgc tgctgacagc cgtgtatgga
      301 ctggtggtgg caggatccgt gctggtgctg ggagcaatca tcggcgactg ggtggataag
      361 aatgccaggc tgaaggtggc ccagaccagc ctggtggtgc agaacgtgtc cgtgatcctg
      421 tgcggcatca tcctgatgat ggtgttcctg cacaagcacg agctgctgac catgtaccac
      481 ggctgggtgc tgacatcttg ttatatcctg atcatcacca tcgccaacat cgccaatctg
      541 gccagcaccg ccacagccat cacaatccag agggactgga tcgtggtggt ggcaggagag
      601 gatcgctcca agctggccaa catgaatgcc acaatccgga gaatcgatca gctgaccaat
      661 atcctggccc ctatggccgt gggccagatc atgacctttg gctctccagt gatcggctgc
      721 ggcttcatct ctggctggaa cctggtgagc atgtgcgtgg agtacgtgct gctgtggaag
      781 gtgtatcaga agacacccgc cctggcagtg aaggcaggcc tgaaggagga ggagaccgag
      841 ctgaagcagc tgaatctgca caaggacaca gagcccaagc ctctggaggg cacccacctg
      901 atgggcgtga aggattctaa catccacgag ctggagcacg agcaggagcc aacatgcgca
      961 agccagatgg cagagccctt ccggaccttt agagacggct gggtgtccta ctataatcag
     1021 cccgtgttcc tggcaggaat gggactggcc tttctgtaca tgacagtgct gggcttcgat
     1081 tgcatcacca caggctacgc ctatacccag ggcctgtctg gcagcatcct gtccatcctg
     1141 atgggcgcct ctgccatcac aggcatcatg ggcaccgtgg cctttacatg gctgaggcgc
     1201 aagtgtggcc tggtgagaac cggactgatc agcggactgg cacagctgtc ctgcctgatc
     1261 ctgtgcgtga tcagcgtgtt catgcctggc agcccactgg acctgtccgt gtctccattc
     1321 gaggatatca ggtctcgctt tatccagggc gagagcatca cccccacaaa gatccctgag
     1381 atcaccacag agatctacat gtccaacggc agcaattccg ccaacatcgt gccagagacc
     1441 agcccagagt ccgtgcctat catctctgtg agcctgctgt ttgcaggcgt gatcgcagca
     1501 aggatcggac tgtggagctt cgacctgacc gtgacacagc tgctgcagga gaatgtgatc
     1561 gagagcgagc gcggcatcat caacggcgtg cagaattcca tgaactacct gctggatctg
     1621 ctgcacttta tcatggtcat cctggcccca aaccccgagg cctttggcct gctggtgctg
     1681 atctccgtgt ctttcgtggc catgggccac atcatgtatt tccggtttgc ccagaataca
     1741 ctgggcaaca agctgttcgc atgtggacca gacgcaaagg aggtgagaaa ggagaatcag
     1801 gccaacacca gcgtggtgta agacccagct ttcttgtaca aagttggcat tataagaaag
     1861 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     1921 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     1981 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     2041 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     2101 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     2161 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     2221 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     2281 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     2341 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     2401 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     2461 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     2521 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     2581 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     2641 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     2701 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     2761 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     2821 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2881 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     2941 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     3001 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3061 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3121 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3181 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3241 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3301 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3361 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3421 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3481 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3541 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3601 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3661 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3721 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3781 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3841 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3901 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     3961 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     4021 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     4081 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     4141 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     4201 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     4261 gggccc
//
