LOCUS       sequence-360560-        4056 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC7A11_STOP
FEATURES             Location/Qualifiers
     source          1..4056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1613..1712)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1729..1748)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1730..1748)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1753..1769)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1753..1769)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1871..1890)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1882..2691
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1949..1968)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2838..3426
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3327..3346
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3580..3597
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3756..3783)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3875..3961)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4010..4032
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4024..4041
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4025..4041
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggt gcggaagccc
      121 gtggtgagca ccatctccaa gggcggctac ctgcagggca acgtgaatgg cagactgcct
      181 tccctgggca ataaggagcc ccctggccag gagaaggtgc agctgaagag gaaggtgacc
      241 ctgctgcgcg gcgtgtctat catcatcggc acaatcatcg gcgccggcat cttcatcagc
      301 ccaaagggcg tgctgcagaa caccggatcc gtgggcatgt ctctgaccat ctggacagtg
      361 tgcggcgtgc tgtccctgtt tggcgccctg tcttacgcag agctgggaac cacaatcaag
      421 aagagcggcg gccactacac ctatatcctg gaggtgttcg gcccactgcc cgcctttgtg
      481 agggtgtggg tggagctgct gatcatcagg ccagcagcaa cagccgtgat ctccctggcc
      541 ttcggccggt atatcctgga gccattcttt atccagtgcg agatccccga gctggccatc
      601 aagctgatca ccgccgtggg catcacagtg gtcatggtgc tgaattctat gagcgtgtcc
      661 tggtctgcca gaatccagat cttcctgacc ttttgtaagc tgacagccat cctgatcatc
      721 atcgtgcctg gcgtgatgca gctgatcaag ggccagaccc agaacttcaa ggacgccttt
      781 tccggccggg atagctccat cacaagactg ccactggcct tctactatgg catgtacgcc
      841 tatgccggct ggttctacct gaactttgtg accgaggagg tggagaatcc cgagaagaca
      901 atccctctgg ccatctgcat cagcatggcc atcgtgacca tcggctacgt gctgacaaac
      961 gtggcctact tcaccacaat caatgccgag gagctgctgc tgtctaacgc cgtggccgtg
     1021 accttcagcg agaggctgct gggcaatttt tccctggccg tgcctatctt cgtggccctg
     1081 agctgttttg gctccatgaa cggcggcgtg ttcgccgtga gcaggctgtt ttatgtggca
     1141 tccagggagg gacacctgcc agagatcctg tctatgatcc acgtgaggaa gcacacccct
     1201 ctgccagccg tgatcgtgct gcaccccctg acaatgatca tgctgttctc tggcgacctg
     1261 gatagcctgc tgaatttcct gagctttgcc cgctggctgt ttatcggact ggcagtggca
     1321 ggactgatct acctgcggta taagtgcccc gacatgcaca gacccttcaa ggtgcctctg
     1381 tttatcccag ccctgttctc ctttacctgt ctgttcatgg tggccctgtc tctgtacagc
     1441 gaccctttct ctacaggcat cggctttgtg atcaccctga caggcgtgcc tgcctactat
     1501 ctgttcatca tctgggataa gaagccacgg tggtttagaa tcatgagcga gaagatcacc
     1561 agaacactgc agatcatcct ggaggtggtg cccgaggagg ataagctgta agacccagct
     1621 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     1681 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag
     1741 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     1801 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     1861 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     1921 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     1981 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2041 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     2101 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     2161 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     2221 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     2281 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     2341 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     2401 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     2461 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     2521 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     2581 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2641 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     2701 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     2761 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     2821 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2881 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2941 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3001 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3061 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3121 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3181 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3241 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3301 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3361 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3421 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3481 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3541 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3601 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     3661 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
     3721 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     3781 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     3841 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     3901 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt
     3961 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
     4021 cgttgtaaaa cgacggccag tcttaagctc gggccc
//
