LOCUS       sequence-360561-        4641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-XPR1_STOP
FEATURES             Location/Qualifiers
     source          1..4641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2198..2297)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2314..2333)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2315..2333)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2338..2354)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2338..2354)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2456..2475)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2467..3276
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2534..2553)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3423..4011
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3912..3931
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4165..4182
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4341..4368)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4460..4546)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4595..4617
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4609..4626
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4610..4626
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgaa gttcgccgag
      121 cacctgtctg cccacatcac ccctgagtgg cggaagcagt atatccagta cgaggccttt
      181 aaggacatgc tgtacagcgc ccaggatcag gcaccatccg tggaggtgac cgacgaggat
      241 acagtgaaga gatatttcgc caagtttgag gagaagttct ttcagacctg cgagaaggag
      301 ctggccaaga tcaacacatt ctactccgag aagctggcag aggcacagcg gagatttgcc
      361 accctgcaga atgagctgca gagctccctg gacgcccaga aggagtctac aggcgtgacc
      421 acactgcggc agaggcgcaa gcccgtgttc cacctgagcc acgaggagag ggtgcagcac
      481 cgcaacatca aggatctgaa gctggccttc tccgagtttt acctgtctct gatcctgctg
      541 cagaattatc agaacctgaa tttcaccggc tttagaaaga tcctgaagaa gcacgacaag
      601 atcctggaga catccagggg agcagattgg agggtggcac acgtggaggt ggccccattc
      661 tatacctgca agaagatcaa ccagctgatc tctgagaccg aggccgtggt gacaaatgag
      721 ctggaggacg gcgataggca gaaggccatg aagaggctga gggtgccacc tctgggagca
      781 gcacagccag caccagcatg gaccacattt cgcgtgggac tgttctgtgg catctttatc
      841 gtgctgaaca tcaccctggt gctggccgcc gtgttcaagc tggagacaga caggagcatc
      901 tggccactga tcaggatcta ccgcggcggc tttctgctga tcgagttcct gtttctgctg
      961 ggcatcaaca cctatggctg gaggcaggcc ggcgtgaatc acgtgctgat cttcgagctg
     1021 aacccccgct ccaatctgtc tcaccagcac ctgttcgaga tcgccggctt tctgggcatc
     1081 ctgtggtgcc tgagcctgct ggcctgtttc tttgccccta tctccgtgat cccaacctac
     1141 gtgtaccccc tggccctgta cggcttcatg gtgttctttc tgatcaaccc aaccaagaca
     1201 ttctactata agagcaggtt ttggctgctg aagctgctgt tccgcgtgtt tacagccccc
     1261 ttccacaaag tgggcttcgc cgacttttgg ctggccgatc agctgaattc cctgtctgtg
     1321 atcctgatgg acctggagta tatgatctgc ttttactctc tggagctgaa gtgggatgag
     1381 agcaagggcc tgctgcccaa caatagcgag gagtccggca tctgtcacaa gtatacctac
     1441 ggcgtgcggg caatcgtgca gtgcatccca gcatggctga gattcatcca gtgtctgcgg
     1501 agataccggg acacaaagag agcctttcct cacctggtga acgccggcaa gtacagcacc
     1561 acattcttta tggtgacctt cgccgccctg tattccacac acaaggagag aggccactct
     1621 gacaccatgg tgttctttta tctgtggatc gtgttttaca tcatctctag ctgctataca
     1681 ctgatctggg acctgaagat ggattggggc ctgttcgata agaacgccgg cgagaatacc
     1741 tttctgcggg aggagatcgt gtatcctcag aaggcctact attactgtgc catcatcgag
     1801 gacgtgatcc tgagattcgc ctggaccatc cagatctcta tcacaagcac cacactgctg
     1861 cctcactccg gcgatatcat cgccaccgtg tttgccccac tggaggtgtt ccggcggttc
     1921 gtgtggaact tctttaggct ggagaatgag cacctgaaca attgtggcga gttccgggcc
     1981 gtgagagaca tcagcgtggc acccctgaac gccgacgatc agaccctgct ggagcagatg
     2041 atggatcagg acgatggcgt gcggaacaga cagaagaatc gctcctggaa gtacaatcag
     2101 agcatctccc tgcggagacc tcggctggcc tctcagagca aggccagaga cacaaaggtg
     2161 ctgatcgagg ataccgacga tgaggccaat acataagacc cagctttctt gtacaaagtt
     2221 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     2281 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     2341 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     2401 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     2461 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     2521 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     2581 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     2641 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     2701 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     2761 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2821 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2881 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2941 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     3001 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     3061 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     3121 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     3181 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     3241 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     3301 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     3361 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3421 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3481 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3541 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3601 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3661 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3721 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3781 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3841 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3901 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3961 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4021 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     4081 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     4141 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     4201 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     4261 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     4321 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     4381 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     4441 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     4501 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     4561 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     4621 gccagtctta agctcgggcc c
//
