LOCUS       sequence-360578-       11865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pHR-R(NFKB-BFP-Triplex-2X 
            crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11865)
  AUTHORS   Kempton HR, Love KS, Guo LY, Qi LS
  TITLE     Scalable biological signal recording in mammalian cells using Cas12a
            base editors.
  JOURNAL   Nat Chem Biol. 2022 May 30. pii: 10.1038/s41589-022-01034-2. doi: 
            10.1038/s41589-022-01034-2.
  PUBMED    35637351
REFERENCE   2  (bases 1 to 11865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 May 30. pii: 10.1038/s41589-022-01034-2. doi: 
            10.1038/s41589-022-01034-2."
COMMENT     Sequence Label: pSLQ11429
FEATURES             Location/Qualifiers
     source          1..11865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          468..533
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             663..683
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1108..1242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             1410..2043
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2090..2215
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2712..2945
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3130..3174
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3459..3576
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4120..4821)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(4890..4914)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        5063..5274
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5285..6307
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6317..6370
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6383..7129
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7164..7752
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7217..7237)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7635..7646)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7836..8069
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8094..8112)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8095..8112)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8402..8421)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8635..8654)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8754..8776
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8814..8832)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8900..9004
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9005..9073,9074..9865)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9223..9242)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10036..10624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10525..10544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10778..10795
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10865..10885)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10870..11199
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11050..11185
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat agtttccagt
       61 aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc
      121 ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc
      181 gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca tgattaaccg
      241 tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat
      301 tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga ttggctaccg
      361 tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct tacttctgtg
      421 gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat
      481 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa
      541 cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt
      601 gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc
      661 ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt
      721 ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa
      781 aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa
      841 gtaggcataa cagttataat cataacatac tgttttttct tactccacac aggcatagag
      901 tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag
      961 gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc
     1021 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     1081 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     1141 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     1201 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
     1261 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
     1321 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
     1381 tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca
     1441 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
     1501 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
     1561 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
     1621 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
     1681 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
     1741 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
     1801 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
     1861 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     1921 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     1981 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     2041 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     2101 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     2161 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     2221 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     2281 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     2341 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     2401 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     2461 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     2521 gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga
     2581 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     2641 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     2701 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     2761 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     2821 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     2881 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     2941 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     3001 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     3061 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     3121 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     3181 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     3241 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     3301 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     3361 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     3421 tctcgacggt atcgccaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     3481 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     3541 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     3601 gatccagttt ggatcgataa gcttgatatc gaatttttta actagactag tcggttagct
     3661 tgagttatcc agttgatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     3721 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     3781 taaccattat aagctgcaat aaacaactcg agaaacaaaa aaaaactcga tctacactta
     3841 gtagaaattt acccttgtta actgaatcga gtatctacac ttagtagaaa tttacccttg
     3901 ttaactgaat cgagtatcta cacttagtag aaattcaaag aaagaaagga tcctcgcaga
     3961 ccaataccag gatcttgcca tcctatggaa ctgcctcggt gagttttgct tttttttttt
     4021 taaagctagg gaaaggccaa aaagcaaaac ctgagaaaac aaaaggttgt tttctcagga
     4081 aaagaaaaac ctttacaacc ctactgacga atcacgcgtt taattaagct tgtgccccag
     4141 tttgctaggg aggtcgcagt atctggccac tgccacctcg tgctgctcga cgtaggtctc
     4201 gttgttggcc tccttgattc tttccagtct gtagtccaca tagtagacgc caggcatctt
     4261 gaggttctta gcgggtttct tggatctata tgtggtcttg atgtttgcga tcagatggct
     4321 cccgcccacg agcttcaggg ccatgtcgtt tctgccttcc aggccgccgt cagcaggata
     4381 cagagtctcg gtgaaggcct cccagccgag tgttttcttc tgcatcacag ggccgttgga
     4441 tgtgaagttc acccctctga tcttgacgtt gtagatgagg cagccgtcct ggaggctggt
     4501 gtcctgggta gcggtcagca cgcccccgtc ttcgtatgtg gtgactctct cccatgtgaa
     4561 gccctcaggg aaggactgct tgaagaagtc ggggatgccc tgggtgtggt tgatgaaggt
     4621 cttgctgccg tagaggaagc tagtagccag gatgtcgaag gcgaagggga gagggccgcc
     4681 ctcgaccacc ttgattctca tggtctgggt gccctcgtag ggcttgcctt cgccctcgga
     4741 tgtgcacttg aagtgatggt tgtccacggt gccctccatg tacagcttca tgtgcatgtt
     4801 ctccttaatc agctcgctca tgaattcagg ctggatcggt cccggtgtct tctatggagg
     4861 tcaaaacagc gtggatggcg tctccaggcg atctgacggt tcactaaacg agctctgctt
     4921 atataggcct cccaccgtac acgcctaggt acctcaagct gaattcggaa agtccccagc
     4981 ggaaagtccc cagcggaaag tccccagcgg aaagtcccca agcggaaagt ccccggatct
     5041 ggtctagagc gcatatgtcg acgggcagag cgcacatcgc ccacagtccc cgagaagttg
     5101 gggggagggg tcggcaattg atccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     5161 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     5221 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggaccgg
     5281 taccatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     5341 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     5401 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     5461 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     5521 cattggggag ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     5581 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     5641 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     5701 aggaatcggt caatacacta catggcgtga tttcatctgc gcgattgctg atccccatgt
     5761 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     5821 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     5881 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     5941 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     6001 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     6061 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     6121 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     6181 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     6241 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     6301 aaaggaaggc ggttccgagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa
     6361 tcctggccca ggcggagggt cgatgagccg gctggacaag agcaaagtga tcaacagcgc
     6421 cctggaactg ctgaacggcg tgggcatcga gggcctgacc acccggaagc tggcccagaa
     6481 actgggcgtg gaacagccca ccctgtactg gcacgtgaag aacaagcggg ccctgctgga
     6541 cgccctgccc atcgagatgc tggaccggca ccacacccac agctgccctc tggaaggcga
     6601 gagctggcag gacttcctgc ggaacaacgc caagagctac agatgcgccc tgctgagcca
     6661 ccgggacggc gccaaagtgc acctgggcac cagacccacc gagaagcagt acgagacact
     6721 ggaaaaccag ctggccttcc tgtgccagca gggcttcagc ctggaaaacg ccctgtacgc
     6781 cctgagcgcc gtgggccact tcaccctggg ctgcgtgctg gaagaacagg aacaccaggt
     6841 cgccaaagag gaaagagaga cacccaccac cgacagcatg ccccccctgc tgaagcaggc
     6901 catcgagctg ttcgacagac agggcgccga gcccgccttc ctgttcggcc tggaactgat
     6961 catctgcggc ctggagaagc agctgaagtg cgagagcggc ggacccaccg acgccctgga
     7021 cgacttcgac ctggacatgc tgcccgccga tgccctggat gattttgatc tggatatgct
     7081 ccctgctgac gctctcgatg acttcgatct cgatatgctg ccaggctaag tttaaacgca
     7141 agcttgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     7201 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     7261 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     7321 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     7381 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     7441 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7501 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     7561 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7621 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7681 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7741 ccgcctcccc gcatgatacc gtcgagtacc tttaagacca atgacttaca aggcagctgt
     7801 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7861 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7921 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7981 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8041 cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag
     8101 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
     8161 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
     8221 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
     8281 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
     8341 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     8401 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     8461 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
     8521 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
     8581 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
     8641 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     8701 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     8761 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     8821 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     8881 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     8941 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     9001 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     9061 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     9121 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     9181 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     9241 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     9301 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     9361 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     9421 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     9481 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     9541 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     9601 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     9661 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     9721 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     9781 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     9841 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     9901 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     9961 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    10021 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    10081 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    10141 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    10201 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    10261 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    10321 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    10381 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    10441 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    10501 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    10561 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    10621 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    10681 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    10741 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    10801 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    10861 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
    10921 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
    10981 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
    11041 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    11101 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
    11161 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
    11221 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
    11281 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
    11341 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
    11401 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
    11461 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta
    11521 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
    11581 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
    11641 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
    11701 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
    11761 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
    11821 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaa
//
