LOCUS       sequence-360579-       14846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pHR-TRE3G-ABE8e-hyperdLbCas12a-NLS-P2A-mCherry-EFS-Puro-WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14846)
  AUTHORS   Kempton HR, Love KS, Guo LY, Qi LS
  TITLE     Scalable biological signal recording in mammalian cells using Cas12a
            base editors.
  JOURNAL   Nat Chem Biol. 2022 May 30. pii: 10.1038/s41589-022-01034-2. doi: 
            10.1038/s41589-022-01034-2.
  PUBMED    35637351
REFERENCE   2  (bases 1 to 14846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 May 30. pii: 10.1038/s41589-022-01034-2. doi: 
            10.1038/s41589-022-01034-2."
COMMENT     Sequence Label: pSLQ11440
FEATURES             Location/Qualifiers
     source          1..14846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             107..340
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(365..383)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(366..383)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(673..692)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(906..925)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1025..1047
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1085..1103)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1171..1275
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1276..1344,1345..2136)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1494..1513)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2307..2895
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2796..2815
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3049..3066
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3136..3156)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3141..3470
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3321..3456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4604..4669
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4799..4819
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5244..5378
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5281..5300)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5335..5354
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5546..6179
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6226..6351
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6848..7081
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7266..7310
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7595..7712
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    7802..7820
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7838..7856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7874..7892
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7910..7928
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7946..7964
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7982..8000
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8018..8036
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     8081..8105
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             8224..8244
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8839..12519
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFRNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIRVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGRGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKRIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     CDS             12520..12567
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             12589..12645
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             12646..13356
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(12791..12809)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     13059..13078
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        13370..13581
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             13592..14191
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(13592..13611)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     14088..14108
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    14255..14843
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(14308..14328)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(14726..14737)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(join(14846,1..16))
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gataccgtcg acctcgaggg aattaattcg agctcggtac ctttaagacc aatgacttac
       61 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
      121 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
      181 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      241 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      301 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt
      361 gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt
      421 agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc
      481 agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct
      541 gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca
      601 tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
      661 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
      721 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
      781 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
      841 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact
      901 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
      961 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     1021 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     1081 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     1141 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     1201 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     1261 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     1321 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     1381 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     1441 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     1501 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     1561 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     1621 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     1681 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     1741 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     1801 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     1861 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     1921 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     1981 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     2041 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     2101 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     2161 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     2221 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     2281 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2341 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2401 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2461 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2521 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2581 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2641 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2701 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2761 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2821 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2881 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     2941 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3001 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3061 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3121 cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     3181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     3241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     3301 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3361 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     3421 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac
     3481 acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc
     3541 agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat
     3601 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
     3661 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
     3721 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
     3781 ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg
     3841 tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag
     3901 aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg
     3961 ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg
     4021 gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc
     4081 cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc
     4141 tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt
     4201 attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc
     4261 tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct
     4321 gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg
     4381 atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg
     4441 atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc
     4501 ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt
     4561 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
     4621 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
     4681 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
     4741 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
     4801 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
     4861 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
     4921 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag
     4981 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc
     5041 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt
     5101 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
     5161 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     5221 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     5281 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5341 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
     5401 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
     5461 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
     5521 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
     5581 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     5641 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     5701 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     5761 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     5821 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     5881 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     5941 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     6001 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6061 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6121 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6181 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     6241 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     6301 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     6361 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     6421 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     6481 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     6541 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     6601 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     6661 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
     6721 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     6781 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     6841 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     6901 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     6961 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     7021 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     7081 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     7141 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     7201 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     7261 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     7321 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     7381 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     7441 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     7501 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
     7561 gacggtatcg ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     7621 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     7681 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     7741 cagtttggat cgataagctt gatatcgaat tcgctgatat ctccacccca cagtggttta
     7801 ctccctatca gtgatagaga acgtatgaag agtttactcc ctatcagtga tagagaacgt
     7861 atgcagactt tactccctat cagtgataga gaacgtataa ggagtttact ccctatcagt
     7921 gatagagaac gtatgaccag tttactccct atcagtgata gagaacgtat ctacagttta
     7981 ctccctatca gtgatagaga acgtatatcc agtttactcc ctatcagtga tagagaacgt
     8041 ataagcttta ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta gtgaaccgtc
     8101 agatcgcctg gagcaattcc acaacacttt tgtcttatac caactttccg taccacttcc
     8161 taccctcgta aacgtacggc cgccaccatg aaacggacag ccgacggaag cgagttcgag
     8221 tcaccaaaga agaagcggaa agtctctgag gtggagtttt cccacgagta ctggatgaga
     8281 catgccctga ccctggccaa gagggcacgg gatgagaggg aggtgcctgt gggagccgtg
     8341 ctggtgctga acaatagagt gatcggcgag ggctggaaca gagccatcgg cctgcacgac
     8401 ccaacagccc atgccgaaat tatggccctg agacagggcg gcctggtcat gcagaactac
     8461 agactgattg acgccaccct gtacgtgaca ttcgagcctt gcgtgatgtg cgccggcgcc
     8521 atgatccact ctaggatcgg ccgcgtggtg tttggcgtga ggaactcaaa aagaggcgcc
     8581 gcaggctccc tgatgaacgt gctgaactac cccggcatga atcaccgcgt cgaaattacc
     8641 gagggaatcc tggcagatga atgtgccgcc ctgctgtgcg atttctatcg gatgcctaga
     8701 caggtgttca atgctcagaa gaaggcccag agctccatca actctggagg atctagcgga
     8761 ggatcctctg gcagcgagac accaggaaca agcgagtcag caacaccaga gagcagtggc
     8821 ggcagcagcg gcggcagcag caagctggag aagtttacaa actgctactc cctgtctaag
     8881 accctgaggt tcaaggccat ccctgtgggc aagacccagg agaacatcga caataagcgg
     8941 ctgctggtgg aggacgagaa gagagccgag gattataagg gcgtgaagaa gctgctggat
     9001 cgctactatc tgtcttttat caacgacgtg ctgcacagca tcaagctgaa gaatctgaac
     9061 aattacatca gcctgttccg gaagaaaacc agaaccgaga aggagaataa ggagctggag
     9121 aacctggaga tcaatctgcg gaaggagatc gccaaggcct tcaagggcaa cgagggctac
     9181 aagtccctgt ttaagaagga tatcatcgag acaatcctgc cagagttcct ggacgataag
     9241 gacgagatcg ccctggtgaa cagcttcaat ggctttacca cagccttcac cggcttcttt
     9301 cggaacagag agaatatgtt ttccgaggag gccaagagca catccatcgc cttcaggtgt
     9361 atcaacgaga atctgacccg ctacatctct aatatggaca tcttcgagaa ggtggacgcc
     9421 atctttgata agcacgaggt gcaggagatc aaggagaaga tcctgaacag cgactatgat
     9481 gtggaggatt tctttgaggg cgagttcttt aactttgtgc tgacacagga gggcatccgc
     9541 gtgtataacg ccatcatcgg cggcttcgtg accgagagcg gcgagaagat caagggcctg
     9601 aacgagtaca tcaacctgta taatcagaaa accaagcaga agctgcctaa gtttaagcca
     9661 ctgtataagc aggtgctgag cgatcgggag tctctgagct tctacggccg gggctataca
     9721 tccgatgagg aggtgctgga ggtgtttaga aacaccctga acaagaacag cgagatcttc
     9781 agctccatca agaagctgga gaagctgttc aagaattttg acgagtactc tagcgccggc
     9841 atctttgtga agaacggccc cgccatcagc acaatctcca agcgtatctt cggcgagtgg
     9901 aacgtgatcc gggacaagtg gaatgccgag tatgacgata tccacctgaa gaagaaggcc
     9961 gtggtgaccg agaagtacga ggacgatcgg agaaagtcct tcaagaagat cggctccttt
    10021 tctctggagc agctgcagga gtacgccgac gccgatctgt ctgtggtgga gaagctgaag
    10081 gagatcatca tccagaaggt ggatgagatc tacaaggtgt atggctcctc tgagaagctg
    10141 ttcgacgccg attttgtgct ggagaagagc ctgaagaaga acgacgccgt ggtggccatc
    10201 atgaaggacc tgctggattc tgtgaagagc ttcgagaatt acatcaaggc cttctttggc
    10261 gagggcaagg agacaaacag ggacgagtcc ttctatggcg attttgtgct ggcctacgac
    10321 atcctgctga aggtggacca catctacgat gccatccgca attatgtgac ccagaagccc
    10381 tactctaagg ataagttcaa gctgtatttt cagaaccctc agttcatggg cggctgggac
    10441 aaggataagg agacagacta tcgggccacc atcctgagat acggctccaa gtactatctg
    10501 gccatcatgg ataagaagta cgccaagtgc ctgcagaaga tcgacaagga cgatgtgaac
    10561 ggcaattacg agaagatcaa ctataagctg ctgcccggcc ctaataagat gctgccaaag
    10621 gtgttctttt ctaagaagtg gatggcctac tataacccca gcgaggacat ccagaagatc
    10681 tacaagaatg gcacattcaa gaagggcgat atgtttaacc tgaatgactg tcacaagctg
    10741 atcgacttct ttaaggatag catctcccgg tatccaaagt ggtccaatgc ctacgatttc
    10801 aacttttctg agacagagaa gtataaggac atcgccggct tttacagaga ggtggaggag
    10861 cagggctata aggtgagctt cgagtctgcc agcaagaagg aggtggataa gctggtggag
    10921 gagggcaagc tgtatatgtt ccagatctat aacaaggact tttccgataa gtctcacggc
    10981 acacccaatc tgcacaccat gtacttcaag ctgctgtttg acgagaacaa tcacggacag
    11041 atcaggctga gcggaggagc agagctgttc atgaggcgcg cctccctgaa gaaggaggag
    11101 ctggtggtgc acccagccaa ctcccctatc gccaacaaga atccagataa tcccaagaaa
    11161 accacaaccc tgtcctacga cgtgtataag gataagaggt tttctgagga ccagtacgag
    11221 ctgcacatcc caatcgccat caataagtgc cccaagaaca tcttcaagat caatacagag
    11281 gtgcgcgtgc tgctgaagca cgacgataac ccctatgtga tcggcatcgc caggggcgag
    11341 cgcaatctgc tgtatatcgt ggtggtggac ggcaagggca acatcgtgga gcagtattcc
    11401 ctgaacgaga tcatcaacaa cttcaacggc atcaggatca agacagatta ccactctctg
    11461 ctggacaaga aggagaagga gaggttcgag gcccgccaga actggacctc catcgagaat
    11521 atcaaggagc tgaaggccgg ctatatctct caggtggtgc acaagatctg cgagctggtg
    11581 gagaagtacg atgccgtgat cgccctggag gacctgaact ctggctttaa gaatagccgc
    11641 gtgaaggtgg agaagcaggt gtatcagaag ttcgagaaga tgctgatcga taagctgaac
    11701 tacatggtgg acaagaagtc taatccttgt gcaacaggcg gcgccctgaa gggctatcag
    11761 atcaccaata agttcgagag ctttaagtcc atgtctaccc agaacggctt catcttttac
    11821 atccctgcct ggctgacatc caagatcgat ccatctaccg gctttgtgaa cctgctgaaa
    11881 accaagtata ccagcatcgc cgattccaag aagttcatca gctcctttga caggatcatg
    11941 tacgtgcccg aggaggatct gttcgagttt gccctggact ataagaactt ctctcgcaca
    12001 gacgccgatt acatcaagaa gtggaagctg tactcctacg gcaaccggat cagaatcttc
    12061 cggaatccta agaagaacaa cgtgttcgac tgggaggagg tgtgcctgac cagcgcctat
    12121 aaggagctgt tcaacaagta cggcatcaat tatcagcagg gcgatatcag agccctgctg
    12181 tgcgagcagt ccgacaaggc cttctactct agctttatgg ccctgatgag cctgatgctg
    12241 cagatgcgga acagcatcac aggccgcacc gacgtggatt ttctgatcag ccctgtgaag
    12301 aactccgacg gcatcttcta cgatagccgg aactatgagg cccaggagaa tgccatcctg
    12361 ccaaagaacg ccgacgccaa tggcgcctat aacatcgcca gaaaggtgct gtgggccatc
    12421 ggccagttca agaaggccga ggacgagaag ctggataagg tgaagatcgc catctctaac
    12481 aaggagtggc tggagtacgc ccagaccagc gtgaagcaca aaaggccggc ggccacgaaa
    12541 aaggccggcc aggcaaaaaa gaaaaaggga tcccatatgg gaagcggagc tactaacttc
    12601 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctatggt gagcaagggc
    12661 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
    12721 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
    12781 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
    12841 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
    12901 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
    12961 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
    13021 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
    13081 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
    13141 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
    13201 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
    13261 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
    13321 cactccaccg gcggcatgga cgagctgtac aagtaagcgg ccgctcgacg ggcagagcgc
    13381 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgatc cggtgcctag
    13441 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
    13501 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
    13561 gggtttgccg ccagaacaca ggaccggtac catgaccgag tacaagccca cggtgcgcct
    13621 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta
    13681 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca
    13741 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
    13801 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
    13861 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
    13921 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
    13981 ttctccagac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
    14041 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
    14101 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
    14161 ctggtgcatg acccgcaagc ccggtgccta aacgcgtggc cgcgactcta gagtcgacct
    14221 gcaggcatgc aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
    14281 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
    14341 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
    14401 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
    14461 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
    14521 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
    14581 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
    14641 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
    14701 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
    14761 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
    14821 ctccctttgg gccgcctccc cgcatc
//
