LOCUS       sequence-360584-       12008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression cassettes for transcription factor induced by estradiol 
            (Lex-ER-B112) or progesterone (ZPM).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12008)
  AUTHORS   Feuer E, Zimran G, Shpilman M, Mosquna A
  TITLE     A Modified Yeast Two-Hybrid Platform Enables Dynamic Control of 
            Expression Intensities to Unmask Properties of Protein-Protein 
            Interactions.
  JOURNAL   ACS Synth Biol. 2022 Jul 27. doi: 10.1021/acssynbio.2c00192.
  PUBMED    35895499
REFERENCE   2  (bases 1 to 12008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2022 Jul 27. doi: 10.1021/acssynbio.2c00192."
COMMENT     Sequence Label: p20 Inducible TFs for G-integration: Lex-ER-B1...
FEATURES             Location/Qualifiers
     source          1..12008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     628..644
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     628..644
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(659..677)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(684..701)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(684..700)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(693..715)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1000..1019)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1132..1153
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1307..1326)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1426..1448
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1486..1504)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1572..1676
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1677..1745,1746..2537)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1895..1914)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2708..3296
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3197..3216
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    3584..3605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    3660..3676
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3665..3687
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3684..3700
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3684..3700
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        4419..4815
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             join(4831..5082,5083..5436)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     5374..5395
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             5455..6390
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5503..6393
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     terminator      6990..7177
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(7045..7069)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            7227..8346
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        7227..7570
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             7571..8143
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      8149..8346
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(8454..8472)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9251..9269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9272..9290
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 catatacaag cactaagaag aacaatacag aactctacac ggtattattg tgctacaagc
       61 tcgagtaaaa ccgagtgttt tgacgatact aacgttgtta agaaagtaac ttgttatcaa
      121 actcattacc aacttgtgat taattggtga ataatatgat aattgtcgaa attccattgt
      181 tggtaaagcc tataatatta tgtatacaga ttatactaga aattctctcg agaatataag
      241 aatccccaaa attgaatcgg tatttctaca tactaatatt accattactt ctcctttcgt
      301 tttatatgtt tcattcctat tacattatcg atctttgcat ttcagcttcc attatatttg
      361 atgtctgttt tatgtcccca cgttacaccg catgtgacag tatactagta acatgagtgc
      421 taccgaatag atgacatttt agactttcat tccaacaact tggttgacag aatgttacgt
      481 accctatatc taatctatat gaggcctgaa tctaactgaa aggtggaatt tcagtaattt
      541 atcaagcttt aataagtttg ggtagtttaa ctgtgcaaaa aggtatttac cttacatact
      601 gaatcttgtc tgtttggtag cggctgctct agaactagtg gatcgatccc caattcgccc
      661 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
      721 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      781 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      841 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
      901 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      961 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
     1021 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggt gggccatcgc
     1081 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     1141 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
     1201 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     1261 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     1321 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     1381 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     1441 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     1501 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     1561 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     1621 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     1681 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     1741 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     1801 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     1861 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     1921 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     1981 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     2041 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     2101 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     2161 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     2221 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     2281 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     2341 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     2401 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     2461 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     2521 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     2581 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     2641 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2701 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2761 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2821 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     2881 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2941 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3001 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3061 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3121 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3181 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3241 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3301 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3361 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3421 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3481 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     3541 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     3601 ctcattaggc accccaggct ttacacttta tgcttccgcg gctcgtatgt tgtgtggaat
     3661 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccactagtcc
     3721 gaggccgcca ggcgccttta tatcatataa ttaagacaca aaaggataaa acaaaggtgt
     3781 taactattct gcatactcac tatcgtaaac tgtcctgcaa atcgtgtaaa tatgtatttc
     3841 attttttttg cagtgaaaaa aggcatgtaa aataccgcat caagtaactc tactccgcct
     3901 gtggtttcaa gactaacggc ttgagacaaa atgggaagaa atgattgcag aaaagccata
     3961 tgtgtaatag caaaaagctg gatagtgctt accagatgtt taccttaatt tcttggtgaa
     4021 ttagagaagt acagaagttt tactattaat cccaccatag aaatttgtat aggaaagtag
     4081 tttattggag ttattggata tactgtgtaa actatttctt gaaattgtaa tcttaagatg
     4141 ctcttcttat tctattaaaa atagaaaatg attttcatat ttatttattt atttatattt
     4201 tggcattact cttcatcatt tttttccctc taagaagctt cctttctttt tataaggata
     4261 acaaaaccaa aaggaatatt gggtcagatg aatggacgcg aatgcaagac agaagtccaa
     4321 atcacgtcaa gacaaagaaa gaaagaaaga aaaactaaca cattaatgta gttttaaaat
     4381 ttcaaatccg aacaacagag catagggttt cgcaaacgca acttcttttc tttttttttc
     4441 ttttctctct cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa tgatggaaga
     4501 cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg
     4561 atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct
     4621 ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaaagt
     4681 ttgctgtctt gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc
     4741 attgttctcg ttccctttct tccttgtttc tttttctgca caatatttca agctatacca
     4801 agcatacaat caactccaag ctttgcaaag atgaaagcgt taacggccag gcaacaagag
     4861 gtgtttgatc tcatccgtga tcacatcagc cagacaggta tgccgccgac gcgtgcggaa
     4921 atcgcgcagc gtttggggtt ccgttcccca aacgcggctg aagaacatct gaaggcgctg
     4981 gcacgcaaag gcgttattga aattgtttcc ggcgcatcac gcgggattcg tctgttgcag
     5041 gaagaggaag aagggttgcc gctggtaggt cgtgtggctg ccggtgaacc acttctggcg
     5101 caacagcata ttgaaggtca ttatcaggtc gatccttcct tattcaagcc gaatgctgat
     5161 ttcctgctgc gcgtcagcgg gatgtcgatg aaagacatcg gcattatgga tggtgacttg
     5221 ctggcagtgc ataaaactca ggatgtacgt aacggtcagg tcgttgtcgc acgtattgat
     5281 gacgaggtta ccgttaagcg cctgaaaaaa cagggcaata aagtcgaact gttgccagaa
     5341 aatagcgagt ttaaaccaat tgtcgtagat cttcgtcagc agagcttcac cattgaaggg
     5401 ctggcggttg gggttattcg caacggcgac tggctgggtt ctaaggatat ctctgctgga
     5461 gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc taagaagaac
     5521 agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga tgctgagccc
     5581 cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc gatgatgggc
     5641 ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc gaagagggtg
     5701 ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg tgcctggtta
     5761 gagatcctga tgattggtct cgtctggcgc tccatggagc acccagtgaa gctactgttt
     5821 gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat ggtggagatc
     5881 ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca gggagaggag
     5941 tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt tctgtccagc
     6001 accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa gatcacagac
     6061 actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca ccagcggctg
     6121 gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg catggagcat
     6181 ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct ggagatgctg
     6241 gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga ggagacggac
     6301 caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa gtattacatc
     6361 acgggggagg cagagggttt ccctgccaca gtcgatatca tgcaggatct gccgggcaac
     6421 gataacagca ccgcgggcga atttccaggt attactttga gaatccaaga aactgatatg
     6481 ttgtataaag gtgatacttt gtatttggat tggttggaag atggtattgc tgaattggtt
     6541 ttcgatgctc caggttctgt taataaattg gatactgctg ttgcttcttt gggtgaagct
     6601 attggtgttt tggaacaaca atctgatttg atttgggaaa ctttgactgt taaagatgct
     6661 aaagttaatt tcgattctgg tttggaaaaa ttcgaagaag ctattccatc tgctgatgat
     6721 ttcgatccag ttgctgaaag aagatcctct ggtgaattta gagctgaaag acattctggt
     6781 ggtactgatt tgtgtttcaa gctttaaacg cgtttaaagc ttatcctcga gacaggcccc
     6841 ttttccaact agtagctttg gacttcttcg ccagaggttt ggtcaagtct ccaatcaagg
     6901 ttgtcggctt gtctaccttg ccagaaattt acgaaaagat ggaaaagggt caaatcgttg
     6961 gtagatacgt tgttgacact tctaaataag cgaatttctt atgatttatg atttttatta
     7021 ttaaataagt tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa
     7081 aacgaaaatt cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat
     7141 agcatgaggt cgctcttatt gaccacacct ctaccggcat gagatctgtt tagcttgcct
     7201 cgtccccgcc gggtcacccg gccagcgaca tggaggccca gaataccctc cttgacagtc
     7261 ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc
     7321 cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt
     7381 acggctcctc gctgcagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt
     7441 gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct
     7501 tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac
     7561 ataaacaacc atgggtacca ctcttgacga cacggcttac cggtaccgca ccagtgtccc
     7621 gggggacgcc gaggccatcg aggcactgga tgggtccttc accaccgaca ccgtcttccg
     7681 cgtcaccgcc accggggacg gcttcaccct gcgggaggtg ccggtggacc cgcccctgac
     7741 caaggtgttc cccgacgacg aatcggacga cgaatcggac gacggggagg acggcgaccc
     7801 ggactcccgg acgttcgtcg cgtacgggga cgacggcgac ctggcgggct tcgtggtcgt
     7861 ctcgtactcc ggctggaacc gccggctgac cgtcgaggac atcgaggtcg ccccggagca
     7921 ccgggggcac ggggtcgggc gcgcgttgat ggggctcgcg acggagttcg cccgcgagcg
     7981 gggcgccggg cacctctggc tggaggtcac caacgtcaac gcaccggcga tccacgcgta
     8041 ccggcggatg gggttcaccc tctgcggcct ggacaccgcc ctgtacgacg gcaccgcctc
     8101 ggacggcgag caggcgctct acatgagcat gccctgcccc taatcagtac tgacaataaa
     8161 aagattcttg ttttcaagaa cttgtcattt gtatagtttt tttatattgt agttgttcta
     8221 ttttaatcaa atgttagcgt gatttatatt ttttttcgcc tcgacatcat ctgcccagat
     8281 gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct
     8341 atactgctgt cgattcgata ctaacgccgc catccagtgt cgaaaacgag ctcgaattca
     8401 tcgatgatat cagatccact agtggcctat gcggccgcgg atctgccggt ctccctatag
     8461 tgagtcgtat taatttcgat aagccaggtt aacctgcatt aatgaatcgg ccaacgcgcg
     8521 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8581 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggccgc catccagtgt
     8641 cgaaaacgac ctcgaattca tcgagtggat tcggctttgg gtaccgggcc cggcaaccaa
     8701 acccatacat cgggattcct ataatacctt cgttggtctc cctaacatgt aggtggcgga
     8761 ggggagatat acaatagaac agataccaga caagacataa tgggctaaac aagactacac
     8821 caattacact gcctcattga tggtggtaca taacgaacta atactgtagc cctagacttg
     8881 atagccatca tcatatcgaa gtttcactac cctttttcca tttgccatct attgaagtaa
     8941 taataggcgc atgcaacttc ttttcttttt ttttcttttc tctctccccc gttgttgtct
     9001 caccatatcc gcaatgacaa aaaaatgatg gaagacacta aaggaaaaat ttaacgacaa
     9061 agacagcacc aacagatgtc gttgttccag agctgatgag gggtatctcg aagcacacga
     9121 aactttttcc ttccttcatt cacgcacact actctctaat gagcaacggt atacggcctt
     9181 ccttccagtt acttgaattt gaaataaaaa aaagtttgct gtcttgctat caagtataaa
     9241 tagacctgaa tccctatcag tgatagagat ctccctatca gtgatagaga ttcttccttg
     9301 tttctttttc tgcaggctag caataaaatg ggtacccgcc catatgcttg ccctgtcgag
     9361 tcctgcgatc gccgcttttc tcgctcggat gagcttaccc gccatatccg catccatacc
     9421 ggtcagaagc ccttccagtg tcgaatctgc atgcgtaact tcagtcgtag tgaccacctt
     9481 accacccaca tccgcaccca cacaggcgag aagccttttg cctgtgacat ttgtgggagg
     9541 aagtttgcca ggagtgatga acgcaagagg cataccaaaa tccatacagg tagatccggt
     9601 gacggtgctg gtttaaagtt caataaagtc agagttgtga gagcactgga tgctgttgct
     9661 ctcccacagc cattgggcgt tccaaatgaa agccaagccc taagccagag attcactttt
     9721 tcaccaggtc aagacataca gttgattcca ccactgatca acctgttaat gagcattgaa
     9781 ccagatgtga tctatgcagg acatgacaac acaaaacctg acacctccag ttctttgctg
     9841 acaagtctta atcaactagg cgagaggcaa cttctttcag tagtcaagtg gtctaaatca
     9901 ttgccaggtt ttcgaaactt acatattgat gaccagataa ctctcattca gtattcttgg
     9961 atgagcttaa tggtgtttgg tctaggatgg agatcctaca aacatgtcag tgggcagatg
    10021 ctgtattttg cacctgatct aatactaaat gaacagcgga tgaaagaatc atcattctat
    10081 tcattatgcc ttaccatgtg gcagatccca caggagtttg tcaagcttca agttagccaa
    10141 gaagagttcc tctgtatgaa agtattgtta cttcttaata caattccttt ggaagggcta
    10201 cgaagtcaaa cccagtttga ggagatgagg tcaagctaca ttagagagct catcaaggca
    10261 attggtttga ggcaaaaagg agttgtgtcg agctcacagc gtttctatca acttacaaaa
    10321 cttcttgata actcgcatga tcttgtcaaa cagcttcatc tgtactgctt gaatacattt
    10381 atccagtccc gggcactgag tgttgaattt ccagaaatga tgtctgaagt tattgctgca
    10441 caattaccca agatattggc agggatggtg aaaccccttc tctttcataa aaaggcggcc
    10501 gcaggtgacg gtgctggttt aattaacatg acggtcgacc atgatttcaa tagcgaagat
    10561 attttattcc ccatagaaag catgagtagt atacaatacg tggagaataa taacccaaat
    10621 aatattaaca acgatgttat cccgtattct ctagatatca aaaacactgt cttagatagt
    10681 gcggatctca atgacattca aaatcaagaa acttcactga atttggggct tcctccacta
    10741 tctttcgact ctccactgcc cgtaacggaa acgataccat ccactaccga taacagcttg
    10801 catttgaaag ctgatagcaa caaaaatcgc gatgcaagaa ctattgaaaa tgatagtgaa
    10861 attaagagta ctaataatgc tagtggctct ggggcaaatc aatacacaac tcttacttca
    10921 ccttatccta tgaacgacat tttgtacaac atgaacaatc cgttacaatc accgtcacct
    10981 tcatcggtac ctcaaaatcc gactataaat cctcccataa atacagcaag taacgaaact
    11041 aatttatcgc ctcaaacttc aaatggtaat gaaactctta tatctcctcg agcccaacaa
    11101 catacgtcca ttaaagataa tcgtctgtcc ttacctaatg gtgctaattc gaatcttttc
    11161 attgacacta acccaaacaa tttgaacgaa aaactaagaa atcaattgaa ctcagataca
    11221 aattcatatt ctaactccat ttctaattca aactccaatt ctacgggtaa tttaaattcc
    11281 agttatttta attcactgaa catagactcc atgctagatg attacgtttc tagtgatctc
    11341 ttattgaatg atgatgatga tgacactaat ttatcacgcc gaagatttag cgacgttata
    11401 acaaaccaag agctctaagc aaatagctaa attatatacg aattaatatt atgattaagt
    11461 gtttacgtga gtgcgatatt tttattacta tcttatacag ttgtatatac tctataaaat
    11521 gagttgtcta ttaattaacg cgatgaatgc tttctgggtt tacctctcca acaactctag
    11581 tttacttctc aatacattca attgtatttg atttgtcaat acttcatcat taatcaattc
    11641 tatagttttg tttttctcgt ttatttccaa atttaatgca tcaattttat tattcaattt
    11701 gtcgttgatt ttggttaatg attttatggt ttgatctctg gcattgattg tttgtgttag
    11761 tttttcatta ttgataatta aattatttaa gttagttatc aactcggtgt tttcaagttt
    11821 caagttttca atttctttag agtttattag atttgtcaaa gtttctgaat tgcttgattg
    11881 gtccggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
    11941 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tcccgtacca atggaaggtc
    12001 gggatgag
//
