LOCUS       sequence-36067-e        8010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Hockemeyer D, Wang H, Kiani S, Lai CS, Gao Q, Cassady JP, Cost GJ, 
            Zhang L, Santiago Y, Miller JC, Zeitler B, Cherone JM, Meng X, 
            Hinkley SJ, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
  TITLE     Genetic engineering of human pluripotent cells using TALE nucleases.
  JOURNAL   Nat Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927.
  PUBMED    21738127
REFERENCE   2  (bases 1 to 8010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927."
COMMENT     Sequence Label: PITX3-2A-eGFP-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..8010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1011..1067
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1068..1070,1071..1073,1074..1787)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1068..1070,1071..1073,1074..1784)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1113..1134)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1374..1393)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1721..1742
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1818..1851
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1912..2408
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1947..1970)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2341..2360
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2448..3047
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2448..2467)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2944..2964
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    3604..3637
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4443..4462)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4444..4462)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4469..4486)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4469..4485)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4478..4500)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4626..5054
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4713..4732)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4923..4944
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             5398..6192
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5452..5471)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6062..6081
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(6210..6278,6279..7070)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6428..6447)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7241..7829
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7730..7749
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7983..8000
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttttcctg
      301 caggcctcat ctcgtttatt gaccgcaggg ccccggctgg aagccccgtc ggggtggccc
      361 gcccttcagc cgctgggacc cggccccggg ccctgaccgc ctttctcccg tgcccgcagg
      421 tgtggttcaa gaaccggcgc gccaaatggc ggaagcgcga gcgcagccag caggccgagc
      481 tatgcaaagg cagcttcgcg gcgccgctcg gggggctggt gccgccctac gaggaggtgt
      541 accccggcta ctcgtacggc aactggccgc ccaaggctct tgccccgccg ctcgccgcca
      601 agacctttcc attcgccttc aactcggtca acgtggggcc tctggcttcg cagcccgtct
      661 tctcgccacc cagctccatc gccgcctcca tggtgccctc cgccgcggct gccccgggca
      721 ccgtgccagg gcctggggcc ctgcagggcc tgggcggggg cccccccggg ctggctccgg
      781 ccgccgtgtc ctccggggcc gtgtcctgcc cttatgcctc ggccgccgcc gccgccgcgg
      841 ctgccgcctc ttccccctac gtctatcggg acccgtgtaa ctcgagcctg gccagcctgc
      901 ggctcaaagc caaacagcac gcctccttca gctaccccgc tgtgcacggg ccgcccccgg
      961 cagccaacct tagtccgtgc cagtacgccg tggaaaggcc cgtagctagc gccactaact
     1021 tctccctgtt gaaacaagca ggggatgtcg aagagaatcc cgggccaatg gtgagcaagg
     1081 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1141 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1201 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     1261 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     1321 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     1381 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1441 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1501 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     1561 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     1621 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     1681 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1741 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagaa ttccgatcat
     1801 attcaataac ccttaatata acttcgtata atgtatgcta tacgaagtta ttaggtctga
     1861 agaggagttt acgtccagcc aagcttagga tctcgacctc gaaattctac cgggtagggg
     1921 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg gcacttggcg
     1981 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtagcg ccaaccggct
     2041 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     2101 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     2161 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     2221 caatagcagc tttgctcctt cgctttctgg gctcagcagc tgggaagggt gggtccgggg
     2281 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     2341 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     2401 gcctttcgac ctgcatccat ctagatctcg agcagctgaa gcttaccatg accgagtaca
     2461 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     2521 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     2581 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     2641 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     2701 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     2761 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     2821 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     2881 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     2941 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     3001 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
     3061 acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcgatgata tcagatcccc
     3121 gggatgcaga aattgatgat ctattaaaca ataaagatgt ccactaaaat ggaagttttt
     3181 cctgtcatac tttgttaaga agggtgagaa cagagtacct acattttgaa tggaaggatt
     3241 ggagctacgg gggtgggggt ggggtgggat tagataaatg cctgctcttt actgaaggct
     3301 ctttactatt gctttatgat aatgtttcat agttggatat cataatttaa acaagcaaaa
     3361 ccaaattaag ggccagctca ttcctcccac tcatgatcta tagatctata gatctctcgt
     3421 gggatcattg tttttctctt gattcccact ttgtggttct aagtactgtg gtttccaaat
     3481 gtgtcagttt catagcctga agaacgagat cagcagcctc tgttccacat acacttcatt
     3541 ctcagtattg ttttgccaag ttctaattcc atcagaagct ggtcgagatc cggaaccctt
     3601 aatataactt cgtataatgt atgctatacg aagttattag gtccctcgaa gaggttcact
     3661 aggcgcgccg cggccccgcc cgtagatcat ccccgagggc gggggcaacg attcacagcc
     3721 tccgcggact ggggtcattt tgactggctt gctcccgccc cagggtctga aaggggtgtt
     3781 tgggcagctg gggggcaccg gctcaggaga gggccttccc ctcccagccc tgaggggtgg
     3841 actaggccct acacacagac cgcgcccctg ggactaaagc caggaacagg gaccagctcc
     3901 ccgggggcca actcaccctt ggcccatccc gccttctcca ggcttcccct ccctcgtttt
     3961 caaagataaa tgaaataaac gtgcgcggac tgtcgaaggc gtgatgattc aaagttgcgc
     4021 ttgagtcctc ttcccttcat cctcccccac tgtctacagg cgagctggat gtacaggaac
     4081 gaagtgaggg tccaggcttc tagggactca gtcccactca ccaccccccc tactccaggt
     4141 cggggcagcc cgggattcct gcagcaaaga ggtgcgtgcg tgcgggggac gtgggaaacc
     4201 ggctacctgg ctggcatcgg ccacagccgg tgctgcggcg ccacctcgtg gtctcaggga
     4261 ggctagctgg tagatctggt ctccaacgct tccttgtagg tgtcagctct gctattcagg
     4321 gagcctatga ctttagataa agttactccg cctttctgca cctggccggc caaaagggcg
     4381 aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga gggcccaatt
     4441 cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg
     4501 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     4561 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     4621 aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4681 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     4741 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4801 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     4861 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4921 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     4981 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5041 atttaacgcg aattttaaca aaattcaggg cgcaagggct gctaaaggaa gcggaacacg
     5101 tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc
     5161 tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg
     5221 cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg
     5281 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg
     5341 atctgatggc gcaggggatc aagatctgat caagagacag gatgaggatc gtttcgcatg
     5401 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     5461 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     5521 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     5581 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     5641 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     5701 ctcctgtcat cccaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     5761 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     5821 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     5881 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     5941 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     6001 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     6061 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     6121 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     6181 gagttcttct gaattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6241 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6301 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6361 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6421 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6481 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6541 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6601 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6661 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6721 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6781 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6841 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6901 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6961 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7021 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7081 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7141 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7201 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7261 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7321 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7381 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7441 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7501 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7561 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7621 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7681 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7741 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7801 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7861 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7921 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7981 gcagcgagtc agtgagcgag gaagcggaag
//
