LOCUS       sequence-360681-        2776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of the Schizosaccharomyces pombe fission yeast 
            calmodulin, Cam1 with the T6C residue replacement to allow 
            labelling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Baker K, Geeves MA, Mulvihill DP
  TITLE     Acetylation stabilises calmodulin-regulated calcium signalling.
  JOURNAL   FEBS Lett. 2022 Jan 31. doi: 10.1002/1873-3468.14304.
  PUBMED    35100446
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2022 Jan 31. doi: 10.1002/1873-3468.14304."
COMMENT     Sequence Label: pJC20_cam1-T6C
FEATURES             Location/Qualifiers
     source          1..2776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     75..94
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        75..93
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             125..147
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(679..697)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      693..740
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(785..803)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        871..975
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(976..1044,1045..1836)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1194..1213)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2007..2595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2496..2515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2749..2766
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaggatct
       61 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaaa
      121 taattttgtt taactttaag aaggagatat acatatgact acccgtaacc tttgtgatga
      181 gcagattgca gaattccgtg aagccttttc actgtttgat cgtgatcagg atggaaatat
      241 cacgtccaat gaattgggtg tagttatgag atcgttaggt caatcgccta ctgccgccga
      301 attacaagat atgattaatg aggtcgatgc cgatggtaat ggcacaattg attttaccga
      361 atttttgact atgatggccc gaaaaatgaa ggataccgac aacgaagagg aagttcgcga
      421 agcctttaaa gtcttcgata aagatggaaa tggatacatt acagtcgagg agttgactca
      481 tgtgcttaca agtcttggtg aacgtttgtc tcaagaagaa gtagccgata tgatacgtga
      541 agccgacacc gatggcgatg gtgtaatcaa ctacgaagaa ttctctcgtg tcatttcttc
      601 caagtagaga tcccgggccc tagctaactg atccggctgc taacaaagcc cgaaaggaag
      661 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      721 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggataattc ttgaagacga
      781 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
      841 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      901 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      961 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     1021 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     1081 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     1141 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     1201 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     1261 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     1321 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     1381 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     1441 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     1501 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     1561 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     1621 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     1681 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     1741 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     1801 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1861 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1921 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1981 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2041 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2101 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2161 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2221 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2281 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2341 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2401 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2461 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2521 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2581 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     2641 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     2701 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     2761 agcgaggaag cggaag
//
