LOCUS       sequence-36070-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  TITLE     Koloteva-Levine et al
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTH339-SUP45(1-138)-GalBD
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..67
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      517..564
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      591..778
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(646..670)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(802..818)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(802..818)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(815..837)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    826..842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tatacgactc actatagggc gagccgccat catggaggag cagaagctga tctcagagga
       61 ggacctgcat atggccatgg agatggataa cgaggttgaa aaaaatattg agatctggaa
      121 ggtcaagaag ttggtccaat ctttagaaaa agctagaggt aatggtactt ctatgatttc
      181 cttagttatt cctcctaagg gtcaaattcc actgtaccaa aaaatgttaa cagatgaata
      241 tggtactgcc tcgaatatta aatctagggt taatcgtctt tccgttttat ctgctatcac
      301 ttccacccaa caaaagttga agctatataa tactttgccc aagaacggtt tagttttata
      361 ttgtggtgat atcatcactg aagatggtaa agaaaaaaag gtcacttttg acatcgaacc
      421 ttacaaacct atcaacacat ccttatattt gtgtgataac aaatttcata cagaagttct
      481 ttcggaattg cttcaataac tgcagcggcc gcataactag cataacccct tggggcctct
      541 aaacgggtct tgaggggttt tttgcgcgct tgcagccaag ctaattccgg gcgaatttct
      601 tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt
      661 aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg
      721 tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca
      781 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
      841 aattccacac aacatacgag ccggaagcat
//
