LOCUS       sequence-360703-        4818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC39A6_STOP
FEATURES             Location/Qualifiers
     source          1..4818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2375..2474)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2491..2510)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2492..2510)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2515..2531)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2515..2531)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2633..2652)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2644..3453
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2711..2730)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3600..4188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4089..4108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4342..4359
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4518..4545)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4637..4723)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4772..4794
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4786..4803
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4787..4803
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc caggaagctg
      121 tctgtgatcc tgatcctgac cttcgccctg agcgtgacaa atcctctgca cgagctgaag
      181 gccgccgcct ttccccagac cacagagaag atctccccta attgggagtc tggcatcaac
      241 gtggatctgg ccatcagcac ccggcagtac cacctgcagc agctgttcta cagatatggc
      301 gagaacaata gcctgtccgt ggagggcttt aggaagctgc tgcagaacat cggcatcgac
      361 aagatcaagc gcatccacat ccaccacgat cacgaccacc acagcgacca cgagcaccac
      421 tccgatcacg agcggcactc tgaccacgag caccacagcg agcatgaaca ccactccgat
      481 cacgaccacc actctcacca caatcacgcc gccagcggca agaacaagag aaaggccctg
      541 tgcccagatc acgactccga tagctccggc aaggatccca ggaattctca gggcaaggga
      601 gcacacaggc ctgagcacgc atccggccgg agaaatgtga aggactctgt gagcgcctcc
      661 gaggtgacct ctacagtgta caacaccgtg agcgagggca cacacttcct ggagaccatc
      721 gagacacctc ggccaggcaa gctgtttcca aaggacgtgt ctagctccac ccccccttcc
      781 gtgacatcta agagcagggt gtctcgcctg gccggcagaa agaccaatga gtccgtgtct
      841 gagcctcgga agggcttcat gtatagcaga aacacaaatg agaacccaca ggagtgtttt
      901 aacgccagca agctgctgac ctcccacggc atgggcatcc aggtgccact gaatgccaca
      961 gagttcaact acctgtgccc cgccatcatc aatcagatcg atgcccggag ctgtctgatc
     1021 cacacctccg agaagaaggc cgagatccca cccaagacat attccctgca gatcgcctgg
     1081 gtgggcggct tcatcgccat cagcatcatc tcctttctgt ctctgctggg cgtgatcctg
     1141 gtgcccctga tgaacagggt gttctttaag ttcctgctga gctttctggt ggccctggca
     1201 gtgggcaccc tgtccggcga cgccttcctg cacctgctgc ctcacagcca cgcatcccac
     1261 caccacagcc actcccacga ggagccagcc atggagatga agcgcggccc cctgttcagc
     1321 cacctgtcta gccagaatat cgaggagtct gcctactttg atagcacctg gaagggactg
     1381 acagccctgg gaggactgta tttcatgttt ctggtggagc acgtgctgac cctgatcaag
     1441 cagtttaagg acaagaagaa gaagaaccag aagaagcctg agaacgacga tgacgtggag
     1501 atcaagaagc agctgtccaa gtacgagtct cagctgagca caaacgagga gaaggtggat
     1561 accgatgaca ggacagaggg ctatctgcgc gccgattccc aggagccttc tcacttcgac
     1621 agccagcagc cagccgtgct ggaggaggag gaagtgatga tcgcccacgc ccacccccag
     1681 gaggtgtaca atgagtatgt gcctagaggc tgcaagaaca agtgtcacag ccactttcac
     1741 gataccctgg gccagtccga tgacctgatc caccaccacc acgactacca ccacatcctg
     1801 catcaccacc accaccagaa tcaccaccca cactctcaca gccagcggta ttccagagag
     1861 gagctgaagg atgcaggagt ggccacactg gcatggatgg tcatcatggg cgatggcctg
     1921 cacaacttct ctgacggact ggcaatcgga gcagccttta ccgagggact gtcctctggc
     1981 ctgtccacat ctgtggccgt gttctgccac gagctgccac acgagctggg cgactttgcc
     2041 gtgctgctga aggccggcat gaccgtgaag caggccgtgc tgtacaatgc cctgtctgcc
     2101 atgctggcct atctgggcat ggccaccggc atcttcatcg gccactacgc cgagaacgtg
     2161 agcatgtgga tcttcgccct gacagccggc ctgtttatgt atgtggccct ggtggacatg
     2221 gtgcccgaga tgctgcacaa tgacgcctcc gatcacggct gttctagatg gggctacttc
     2281 tttctgcaga acgccggcat gctgctgggc ttcggcatca tgctgctgat ctccatcttt
     2341 gagcacaaga tcgtgttccg catcaacttt taagacccag ctttcttgta caaagttggc
     2401 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     2461 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     2521 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     2581 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     2641 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     2701 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     2761 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     2821 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     2881 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     2941 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     3001 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     3061 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     3121 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     3181 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     3241 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     3301 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     3361 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     3421 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     3481 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     3541 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3601 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3661 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3721 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3781 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3841 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3901 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3961 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4021 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4081 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4141 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4201 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4261 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     4321 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     4381 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     4441 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     4501 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     4561 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4621 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4681 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4741 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4801 agtcttaagc tcgggccc
//
