LOCUS       sequence-360704-        4029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC39A14_STOP
FEATURES             Location/Qualifiers
     source          1..4029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1586..1685)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1702..1721)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1703..1721)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1726..1742)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1726..1742)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1844..1863)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1855..2664
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1922..1941)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2811..3399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3300..3319
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3553..3570
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3729..3756)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3848..3934)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3983..4005
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3997..4014
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3998..4014
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgaa gctgctgctg
      121 ctgcaccctg cctttcagag ctgtctgctg ctgaccctgc tgggactgtg gaggaccaca
      181 ccagaggcac acgcaagctc cctgggagca ccagccatca gcgccgcctc cttcctgcag
      241 gacctgatcc acagatacgg cgagggcgat agcctgaccc tgcagcagct gaaggccctg
      301 ctgaatcacc tggacgtggg agtgggaagg ggaaacgtga cacagcacgt gcagggccac
      361 agaaatctgt ccacctgctt ttctagcggc gatctgttta cagcccacaa cttctctgag
      421 cagagcagga tcggctcctc tgagctgcag gagttctgtc caaccatcct gcagcagctg
      481 gactccaggg cctgcacatc tgagaaccag gagaatgagg agaacgagca gaccgaggag
      541 ggccgcccat ctgccgtgga agtgtgggga tacggactgc tgtgcgtgac agtgatctcc
      601 ctgtgctctc tgctgggagc atccgtggtg ccctttatga agaagacctt ctacaagcgc
      661 ctgctgctgt acttcatcgc cctggccatc ggcacactgt attccaacgc cctgttccag
      721 ctgatccccg aggcctttgg cttcaatcct ctggaggact actacgtgag caagagcgcc
      781 gtggtgtttg gcggcttcta cctgttcttt ttcacagaga agatcctgaa gatcctgctg
      841 aagcagaaga acgagcacca ccacggccac agccactatg cctccgagtc tctgccctcc
      901 aagaaggatc aggaggaggg cgtgatggag aagctgcaga atggcgacct ggatcacatg
      961 atccctcagc actgtagctc cgagctggac ggcaaggccc caatggtgga tgagaaagtg
     1021 atcgtgggaa gcctgtccgt gcaggacctg caggcctctc agagcgcctg ctactggctg
     1081 aagggcgtgc ggtattccga tatcggcacc ctggcctgga tgatcacact gtctgacggc
     1141 ctgcacaact tcatcgatgg cctggccatc ggcgcctctt ttaccgtgag cgtgttccag
     1201 ggcatctcca catctgtggc catcctgtgc gaggagtttc ctcacgagct gggcgatttc
     1261 gtgatcctgc tgaacgccgg catgtctatc cagcaggccc tgtttttcaa ttttctgagc
     1321 gcctgctgtt gctacctggg actggccttc ggcatcctgg caggcagcca cttttccgcc
     1381 aattggatct tcgccctggc cggcggcatg tttctgtata tcagcctggc cgacatgttc
     1441 cccgagatga acgaggtgtg ccaggaggat gagcggaagg gctccatcct gatccctttt
     1501 atcatccaga atctgggcct gctgaccggc ttcacaatca tggtggtgct gaccatgtat
     1561 agcggccaga tccagatcgg ctaagaccca gctttcttgt acaaagttgg cattataaga
     1621 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     1681 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     1741 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     1801 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     1861 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     1921 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     1981 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     2041 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     2101 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     2161 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     2221 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     2281 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     2341 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     2401 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     2461 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     2521 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     2581 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     2641 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     2701 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     2761 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2821 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2881 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2941 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3001 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3061 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3121 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3181 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3241 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3301 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3361 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3421 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3481 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3541 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     3601 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     3661 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     3721 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     3781 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     3841 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     3901 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     3961 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     4021 ctcgggccc
//
