LOCUS       sequence-36071-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Soellu C, Pars K, Cornu TI, Thibodeau-Beganny S, Maeder ML, Joung 
            JK, Heilbronn R, Cathomen T
  TITLE     Autonomous zinc-finger nuclease pairs for targeted chromosomal 
            deletion.
  JOURNAL   Nucleic Acids Res. 2010 Dec 1. 38(22):8269-76.
  PUBMED    20716517
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2010 Dec 1. 38(22):8269-76."
COMMENT     Sequence Label: pMG10
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..55)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(71..658)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     CDS             complement(699..719)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(726..791)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(798..851)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gatgaccggt acgcgtagaa tcgagaccga ggagagggtt agggataggc ttaccttcga
       61 agggccctta aaagtttatc tcgccgttat taaatttccg tctcacttcc tctaaggtta
      121 atgtgccggc tttaatcatt tctccaccaa ttaaaagctc ttctacacta agaacagctc
      181 cattacaatt agtgatatga tttaatcgtg taagctgagc tttgtagttt cctttaaagt
      241 gaccactcac aaataaaaac ttaaattccg ttacagaaga tggatagact ttccaccatt
      301 cattagggtt gagatgtttg tttcgtgttt gattttcttc gacatatcgc tccatttcat
      361 ctgcttggcc aattggcaga ttataacctc cgctataagc tttagtatcc acgatcacac
      421 cgtaatcaat aggagatccg acagtataaa ttgctccgtc cggtttcctt gatccaccca
      481 aatgtttacc tctatatcca taaactttca taaaaaattc cattaccttc atttcaagaa
      541 ttctatcctg agtggaattt ctggcaattt caattaattc aatatattca tgaggcacat
      601 atttcaattt atgacgaagt tcagatttct tctcctccag ttcacttttg actagttggg
      661 atccaagctt actatctaga cggcaccccg tgaatgccca ccttcctctt cttcttgggg
      721 gccatcttgt catcgtcatc cttgtaatcg atgtcatgat ctttataatc accgtcatgg
      781 tctttgtagt ccatcctagg gccgggattc tcctccacgt caccgcatgt tagaagactt
      841 cctctgccct ctccgccgcc ggacctgaaa ttgatttcac cattgttgaa cttgcggcgc
      901 acctcttcca gggtcagggt accagccttg atcatctcgc c
//
