LOCUS       sequence-360719-       10528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA 
            pre-cloned. Selection =SplitHygromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10528)
  AUTHORS   Bean BDM, Whiteway M, Martin VJJ
  TITLE     The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and 
            Overexpression CRISPR-Cas9 Toolkit.
  JOURNAL   G3 (Bethesda). 2022 Jun 16. pii: 6609175. doi: 
            10.1093/g3journal/jkac154.
  PUBMED    35708612
REFERENCE   2  (bases 1 to 10528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Jun 16. pii: 6609175. doi: 
            10.1093/g3journal/jkac154."
COMMENT     Sequence Label: pBBK22 pCAS-Tyr-[gRNA: 5=ARS308] (SplitHygR, A...
FEATURES             Location/Qualifiers
     source          1..10528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(962..1009)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_RNA        1382..1457
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1591..1933
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      3479..3676
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(join(3827..4618,4619..4687))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4450..4469
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4688..4792)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5495..9598
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5495..9598
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9608..9628
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9644..9667
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      9671..9860
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(9917..10505)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9997..10016)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc taatttttca
       61 aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag cgctatttta
      121 ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag agcgctaatt
      181 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gagagcgcta
      241 ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc ccgagagcgc
      301 tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct ctataatgca
      361 gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc tactttggtg
      421 tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact gattactagc
      481 gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct ataccgatgt
      541 ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca ttggtcagaa
      601 aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa tgtttacatt
      661 ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt tgtctaaaga
      721 gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag
      781 cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag caaagagata
      841 cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagcccg ttacagtccg
      901 gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc aaaagcgctc
      961 tgaagttcct atactttcta gagaatagga acttcggaat aggaacttca aagcgtttcc
     1021 gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca ctgttcacgt
     1081 cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg gcatagtgcg
     1141 tgtttatgct taaatgcgta tatgtgttat gtagtatact ctttcttcaa caattaaata
     1201 ctctcggtag ccaagttggt ttaaggcgca agactgtaat ttatcactac gaaatcttga
     1261 gatcgggcgt tcgactcgcc cccgggagag atggccggca tggtcccagc ctcctcgctg
     1321 gcgccggctg ggcaacacct tcgggtggcg aatgggactt tcacttgtca aacagaatat
     1381 agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
     1441 tggcaccgag tcggtgcttt ttttattttt tgtcactatt gttatgtaaa atgccacctc
     1501 tgacagtatg gaacgcaaac ttctgtctag tggatatagg tctagagatc tgtttagctt
     1561 gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac
     1621 agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata
     1681 catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa
     1741 aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg
     1801 aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg
     1861 ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc
     1921 gaacataaac aacaatggga aagaaaccag aattgacagc tacgtctgtc gagaagtttc
     1981 tgatcgaaaa gttcgacagc gtgtccgacc tgatgcagct ctcggagggc gaagaatctc
     2041 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     2101 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     2161 cggaagtgct tgacattggg gaatttagcg agagcctgac ctattgcatc tcccgccgtg
     2221 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcaaccgg
     2281 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     2341 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     2401 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     2461 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     2521 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     2581 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     2641 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     2701 agcttgcagg atcgacgtcg ctgcctgcag gacagggtgt cacgttgcaa gacctgcctg
     2761 aaaccgaact gcccgctgtt ctgcaaccgg tcgcggaggc catggatgcg atcgctgcgg
     2821 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
     2881 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
     2941 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
     3001 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
     3061 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
     3121 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
     3181 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
     3241 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
     3301 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
     3361 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
     3421 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa taatcagtac
     3481 tgacaataaa aagattcttg ttttcaagaa cttgtcattt gtatagtttt tttatattgt
     3541 agttgttcta ttttaatcaa atgttagcgt gatttatatt ttttttcgcc tcgacatcat
     3601 ctgcccagat gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc gtatgtgaat
     3661 gctggtcgct atactgctgt cgattcgata ctaacgccgc catccagtgt cgaaaacgag
     3721 ctctcgagaa cccttaatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3781 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3841 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3901 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3961 taccgcggga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4021 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4081 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4141 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4201 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4261 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4321 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4381 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4441 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4501 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4561 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4621 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4681 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4741 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttcgt cgacagtcga
     4801 acaagaagca ggcaaagttt agagcactgc ccctccgcac tcaaaaaaga aaaaactagg
     4861 aggaaaataa aattctcaac cacacaaaca cataaacaca tacaaataca aatacaagct
     4921 tatttacttg acatcgcgcg atcttccact attcagcgcc gtccgccctc tctcgtgttt
     4981 tttgtttacg cgacaactat gcgaaatccg gagcaacggg caaccgtttg gggaaagacc
     5041 acacccacgc gcgatcgcca tggcaacgag gtcgcacacg ccccacaccc agacctccct
     5101 gcgagcgggc atgggtacaa tgtccccgtt gccacagaca ccacttcgta gcacagcgca
     5161 gagcgtagcg tgttgttgct gctgacaaaa gaaaattttt cttagcaaag caaaggaggg
     5221 gaagcacggg cagatagcac cgtaccatac ccttggaaac tcgaaatgaa cgaagcagga
     5281 aatgagagaa tgagagtttt gtaggtatat atagcggtag tgtttgcgcg ttaccatcat
     5341 cttctggatc tatctattgt tcttttcctc atcactttcc cctttttcgc tcttcttctt
     5401 gtcttttatt tctttctttt ttttaattgt tccctcgatt ggctatctac caaagaatcc
     5461 aaacttaata cacgtattta tttgtccaat taccatggat aagaaatact caataggctt
     5521 agatatcggc acaaatagcg tcggatgggc ggtgatcact gatgaatata aggttccgtc
     5581 taaaaagttc aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa atcttatagg
     5641 ggctctttta tttgacagtg gagagacagc ggaagcgact cgactcaaac ggacagctcg
     5701 tagaaggtat acacgtcgga agaatcgtat ttgttatcta caggagattt tttcaaatga
     5761 gatggcgaaa gtagatgata gtttctttca tcgacttgaa gagtcttttt tggtggaaga
     5821 agacaagaag catgaacgtc atcctatttt tggaaatata gtagatgaag ttgcttatca
     5881 tgagaaatat ccaactatct atcatctgcg aaaaaaattg gtagattcta ctgataaagc
     5941 ggatttgcgc ttaatctatt tggccttagc gcatatgatt aagtttcgtg gtcatttttt
     6001 gattgaggga gatttaaatc ctgataatag tgatgtggac aaactattta tccagttggt
     6061 acaaacctac aatcaattat ttgaagaaaa ccctattaac gcaagtggag tagatgctaa
     6121 agcgattctt tctgcacgat tgagtaaatc aagacgatta gaaaatctca ttgctcagct
     6181 ccccggtgag aagaaaaatg gcttatttgg gaatctcatt gctttgtcat tgggtttgac
     6241 ccctaatttt aaatcaaatt ttgatttggc agaagatgct aaattacagc tttcaaaaga
     6301 tacttacgat gatgatttag ataatttatt ggcgcaaatt ggagatcaat atgctgattt
     6361 gtttttggca gctaagaatt tatcagatgc tattttactt tcagatatcc taagagtaaa
     6421 tactgaaata actaaggctc ccctatcagc ttcaatgatt aaacgctacg atgaacatca
     6481 tcaagacttg actcttttaa aagctttagt tcgacaacaa cttccagaaa agtataaaga
     6541 aatctttttt gatcaatcaa aaaacggata tgcaggttat attgatgggg gagctagcca
     6601 agaagaattt tataaattta tcaaaccaat tttagaaaaa atggatggta ctgaggaatt
     6661 attggtgaaa ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg acaacggctc
     6721 tattccccat caaattcact tgggtgagct gcatgctatt ttgagaagac aagaagactt
     6781 ttatccattt ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt ttcgaattcc
     6841 ttattatgtt ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc
     6901 tgaagaaaca attaccccat ggaattttga agaagttgtc gataaaggtg cttcagctca
     6961 atcatttatt gaacgcatga caaactttga taaaaatctt ccaaatgaaa aagtactacc
     7021 aaaacatagt ttgctttatg agtattttac ggtttataac gaattgacaa aggtcaaata
     7081 tgttactgaa ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga aagccattgt
     7141 tgatttactc ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag aagattattt
     7201 caaaaaaata gaatgttttg atagtgttga aatttcagga gttgaagata gatttaatgc
     7261 ttcattaggt acctaccatg atttgctaaa aattattaaa gataaagatt ttttggataa
     7321 tgaagaaaat gaagatatct tagaggatat tgttttaaca ttgaccttat ttgaagatag
     7381 ggagatgatt gaggaaagac ttaaaacata tgctcacctc tttgatgata aggtgatgaa
     7441 acagcttaaa cgtcgccgtt atactggttg gggacgtttg tctcgaaaat tgattaatgg
     7501 tattagggat aagcaatctg gcaaaacaat attagatttt ttgaaatcag atggttttgc
     7561 caatcgcaat tttatgcagc tgatccatga tgatagtttg acatttaaag aagacattca
     7621 aaaagcacaa gtgtctggac aaggcgatag tttacatgaa catattgcaa atttagctgg
     7681 tagccctgct attaaaaaag gtattttaca gactgtaaaa gttgttgatg aattggtcaa
     7741 agtaatgggg cggcataagc cagaaaatat cgttattgaa atggcacgtg aaaatcagac
     7801 aactcaaaag ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa
     7861 agaattagga agtcagattc ttaaagagca tcctgttgaa aatactcaat tgcaaaatga
     7921 aaagctctat ctctattatc tccaaaatgg aagagacatg tatgtggacc aagaattaga
     7981 tattaatcgt ttaagtgatt atgatgtcga tcacattgtt ccacaaagtt tccttaaaga
     8041 cgattcaata gacaataagg tcttaacgcg ttctgataaa aatcgtggta aatcggataa
     8101 cgttccaagt gaagaagtag tcaaaaagat gaaaaactat tggagacaac ttctaaacgc
     8161 caagttaatc actcaacgta agtttgataa tttaacgaaa gctgaacgtg gaggtttgag
     8221 tgaacttgat aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa
     8281 gcatgtggca caaattttgg atagtcgcat gaatactaaa tacgatgaaa atgataaact
     8341 tattcgagag gttaaagtga ttaccttaaa atctaaatta gtttctgact tccgaaaaga
     8401 tttccaattc tataaagtac gtgagattaa caattaccat catgcccatg atgcgtatct
     8461 aaatgccgtc gttggaactg ctttgattaa gaaatatcca aaacttgaat cggagtttgt
     8521 ctatggtgat tataaagttt atgatgttcg taaaatgatt gctaagtctg agcaagaaat
     8581 aggcaaagca accgcaaaat atttctttta ctctaatatc atgaacttct tcaaaacaga
     8641 aattacactt gcaaatggag agattcgcaa acgccctcta atcgaaacta atggggaaac
     8701 tggagaaatt gtctgggata aagggcgaga ttttgccaca gtgcgcaaag tattgtccat
     8761 gccccaagtc aatattgtca agaaaacaga agtacagaca ggcggattct ccaaggagtc
     8821 aattttacca aaaagaaatt cggacaagct tattgctcgt aaaaaagact gggatccaaa
     8881 aaaatatggt ggttttgata gtccaacggt agcttattca gtcctagtgg ttgctaaggt
     8941 ggaaaaaggg aaatcgaaga agttaaaatc cgttaaagag ttactaggga tcacaattat
     9001 ggaaagaagt tcctttgaaa aaaatccgat tgacttttta gaagctaaag gatataagga
     9061 agttaaaaaa gacttaatca ttaaactacc taaatatagt ctttttgagt tagaaaacgg
     9121 tcgtaaacgg atgctggcta gtgccggaga attacaaaaa ggaaatgagc tggctctgcc
     9181 aagcaaatat gtgaattttt tatatttagc tagtcattat gaaaagttga agggtagtcc
     9241 agaagataac gaacaaaaac aattgtttgt ggagcagcat aagcattatt tagatgagat
     9301 tattgagcaa atcagtgaat tttctaagcg tgttatttta gcagatgcca atttagataa
     9361 agttcttagt gcatataaca aacatagaga caaaccaata cgtgaacaag cagaaaatat
     9421 tattcattta tttacgttga cgaatcttgg agctcccgct gcttttaaat attttgatac
     9481 aacaattgat cgtaaacgat atacgtctac aaaagaagtt ttagatgcca ctcttatcca
     9541 tcaatccatc actggtcttt atgaaacacg cattgatttg agtcagctag gaggtgacgg
     9601 tggagggcca aaaaagaaaa gaaaagttga agatgcttct gctcatcatc accatcacca
     9661 tcaccattaa atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc
     9721 ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc
     9781 ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga
     9841 aggttttggg acgctcgaag tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9901 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9961 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    10021 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    10081 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    10141 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    10201 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    10261 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    10321 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    10381 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    10441 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    10501 ctcaagaaga tcctttgatc ttttctac
//
