LOCUS       sequence-360733-        8324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LentiGuide-puro vector expressing the sgRNA necessary for 
            linearization of the donor cassette plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8324)
  AUTHORS   Serebrenik YV, Sansbury SE, Kumar SS, Henao-Mejia J, Shalem O
  TITLE     Efficient and flexible tagging of endogenous genes by 
            homology-independent intron targeting.
  JOURNAL   Genome Res. 2019 Aug;29(8):1322-1328. doi: 10.1101/gr.246413.118. 
            Epub 2019 Jun 25.
  PUBMED    31239279
REFERENCE   2  (bases 1 to 8324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.246413.118"; journalName: "Genome Res"; date: "2019-08";
            volume: "29"; issue: "8"; pages: "1322-1328"
COMMENT     Sequence Label: sgDonor_lentiGuide
FEATURES             Location/Qualifiers
     source          1..8324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      666..1254
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1155..1174
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1408..1425
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1542..1563
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1578..1583,1584..1601,1602..1608)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1616..1632
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1621..1643
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1640..1656
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1640..1656
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1675..1695
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1677..1695
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1723..1949
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1950..2130
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2177..2302
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2795..3028
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3213..3257
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        3436..3684
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3436..3456
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3607..3626
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        3706..3781
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    3837..3954
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4093..5271
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4324..5262
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5219..5239
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5284..5883
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5284..5303)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5780..5800
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5899..6487
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5952..5972)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6370..6381)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6559..6792
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6870..6991
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6907..6926)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6961..6980
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7031..7166
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7186..7205)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7187..7205)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7215..7232)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7215..7231)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7224..7246)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7373..7828
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7460..7479)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7670..7691
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7854..7958
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7959..8027,8028..8324,1..495)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8177..8196)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
       61 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
      121 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
      181 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
      241 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
      301 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
      361 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
      421 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
      481 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
      541 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
      601 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
      661 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
      721 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
      781 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
      841 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      901 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      961 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     1021 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     1081 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     1141 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     1201 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     1261 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     1321 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     1381 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     1441 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     1501 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     1561 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     1621 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     1681 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     1741 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     1801 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     1861 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     1921 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     1981 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     2041 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     2101 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     2161 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     2221 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     2281 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     2341 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     2401 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     2461 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     2521 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     2581 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     2641 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     2701 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     2761 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     2821 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     2881 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     2941 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     3001 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     3061 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     3121 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     3181 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     3241 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     3301 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     3361 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagaccacc tcccaacccc
     3421 gagggcgggc ttgtcgaggg cctatttccc atgattcctt catatttgca tatacgatac
     3481 aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa
     3541 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
     3601 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
     3661 atcttgtgga aaggacgaaa caccgaagag cgaatcgatt tcgtggtttt agagctagaa
     3721 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
     3781 cttttttaag cttggcgtaa ctagatcttg agacaaatgg cagtattcat ccacaatttt
     3841 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     3901 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     3961 tattacaggg acagcagaga tccactttgg cgccggctcg agggggcccg ggtgcaaaga
     4021 tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa
     4081 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     4141 gagaagttgg ggggaggggt cggcaattga tccggtgcct agagaaggtg gcgcggggta
     4201 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     4261 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     4321 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     4381 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
     4441 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
     4501 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     4561 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     4621 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
     4681 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     4741 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     4801 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     4861 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
     4921 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     4981 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     5041 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     5101 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     5161 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     5221 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg acgtacggcc
     5281 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     5341 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     5401 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     5461 gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg gaccacgccg
     5521 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     5581 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     5641 gagcccgcgt ggttcctggc caccgtcgga gtctcgcccg accaccaggg caagggtctg
     5701 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     5761 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     5821 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     5881 tgaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     5941 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6001 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6061 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6121 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6181 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6241 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     6301 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6361 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6421 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6481 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6541 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     6601 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6661 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6721 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6781 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     6841 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     6901 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6961 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     7021 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7081 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     7141 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     7201 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     7261 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     7321 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     7381 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     7441 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     7501 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7561 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7621 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7681 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7741 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7801 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     7861 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7921 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7981 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8041 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8101 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8161 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8221 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8281 aaaagcatct tacggatggc atgacagtaa gagaattatg cagt
//
